Restriction map for
pFN21AB8316
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccI AccII AccIII AclI AflII
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eco81I EcoNI
EcoRV EcoT22I FseI FspI HincII
HindII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1098 GGTACC G^GTACC
AflIII 728 ACACGT A^C[GA][CT]GT
AgeI 1101 ACCGGT A^CCGGT
AhdI 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 948 AGCGCT AGC^GCT
ApaI 1231 GGGCCC GGGCC^C
Asp718I 1098 GGTACC G^GTACC
AspEI 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1263 GAGCAC G[AT]GC[AT]^C
AspI 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 150 CCCGGG C^[CT]CG[GA]G
BamHI 688 GGATCC G^GATCC
BclI 374 TGATCA T^GATCA
BsiHKAI 1263 GAGCAC G[AT]GC[AT]^C
BsmBI 731 CGTCTC CGTCTC(1/5)
BsoBI 150 CCCGGG C^[CT]CG[GA]G
BsrDI 1183 CATTGC GCAATG(2/0)
BssSI 613 CTCGTG CACGAG(-5/-1)
BstAPI 1129 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1104 GGTGACC G^GT[ACGT]ACC
BstPI 1104 GGTGACC G^GT[ACGT]ACC
CfoI 949 GCGC GCG^C
DraIII 424 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 247 GACGGTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 148 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 948 AGCGCT AGC^GCT
EcoO65I 1104 GGTGACC G^GT[ACGT]ACC
EcoRI 990 GAATTC G^AATTC
FbaI 374 TGATCA T^GATCA
HaeII 948 AGCGCT [GA]GCGC^[CT]
HhaI 949 GCGC GCG^C
HinP1I 949 GCGC G^CGC
HindIII 221 AAGCTT A^AGCTT
KpnI 1098 GGTACC GGTAC^C
MspA1I 929 CCGCTG C[AC]G^C[GT]G
NheI 1222 GCTAGC G^CTAGC
PflFI 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1101 ACCGGT A^CCGGT
PleI 598 GACTC GAGTC(4/5)
PpuMI 511 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 859 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 944 CTGCAG CTGCA^G
SexAI 749 ACCAGGT A^CC[AT]GGT
SfcI 944 CTGCAG C^T[GA][CT]AG
SmaI 150 CCCGGG CCC^GGG
SmlI 174 CTTGAG C^T[CT][GA]AG
SphI 1073 GCATGC GCATG^C
Tth111I 748 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 150 CCCGGG C^CCGGG
XmaI 150 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 253 GACGTC GACGT^C
415 GACGTC
AcsI 45 AAATTC [GA]^AATT[CT]
990 GAATTC
ApoI 45 AAATTC [GA]^AATT[CT]
990 GAATTC
BanII 165 GAGCCC G[GA]GC[CT]^C
1231 GGGCCC
BfaI 1111 CTAG C^TAG
1223 CTAG
BsaI 106 GAGACC GGTCTC(1/5)
546 GGTCTC
BsaWI 921 TCCGGT [AT]^CCGG[AT]
1101 ACCGGT
BsiEI 776 CGGCCG CG[GA][CT]^CG
881 CGGCCG
DdeI 713 CTGAG C^T[ACGT]AG
983 CTCAG
DraII 511 AGGACCT [GA]G^G[ACGT]CC[CT]
1231 GGGCCCC
EagI 776 CGGCCG C^GGCCG
881 CGGCCG
EarI 624 GAAGAG CTCTTC(1/4)
629 GAAGAG
EclXI 776 CGGCCG C^GGCCG
881 CGGCCG
Eco52I 776 CGGCCG C^GGCCG
881 CGGCCG
EcoO109I 511 AGGACCT [GA]G^G[ACGT]CC[CT]
1231 GGGCCCC
EcoT14I 1043 CCATGG C^C[AT][AT]GG
1145 CCATGG
FauI 258 CCCGC CCCGC(4/6)
521 CCCGC
HgaI 495 GCGTC GACGC(5/10)
1024 GCGTC
Ksp632I 624 GAAGAG CTCTTC(1/4)
629 GAAGAG
MaeI 1111 CTAG C^TAG
1223 CTAG
NaeI 879 GCCGGC GCC^GGC
883 GCCGGC
NcoI 1043 CCATGG C^CATGG
1145 CCATGG
NgoMIV 879 GCCGGC G^CCGGC
883 GCCGGC
NspI 1073 GCATGC [GA]CATG^[CT]
1214 ACATGC
PflMI 204 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
341 CCATCTGGTGG
StyI 1043 CCATGG C^C[AT][AT]GG
1145 CCATGG
TaqI 49 TCGA T^CGA
445 TCGA
TfiI 402 GATTC G^A[AT]TC
841 GATTC
Tsp509I 46 AATT ^AATT
991 AATT
TthHB8I 49 TCGA T^CGA
445 TCGA
Van91I 204 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
341 CCATCTGGTGG
XcmI 298 CCACAGAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
792 CCAAATCAGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AvaII BanI BbvI BcnI
BmyI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr10I Cfr13I DpnII
DsaI EaeI EcoRII Fnu4HI FokI
HaeIII HapII Hin1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI XhoII