Restriction map for
pFN21AE0591
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbvCI BciVI BclI BfaI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MunI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcsI 661 AAATTC [GA]^AATT[CT]
AfaI 825 GTAC GT^AC
AlwNI 1217 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 927 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1187 AGCGCT AGC^GCT
ApoI 661 AAATTC [GA]^AATT[CT]
BbeI 223 GGCGCC GGCGC^C
BbrPI 692 CACGTG CAC^GTG
BcgI 366 CGACTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 95 AGATCT A^GATCT
BlpI 1227 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1227 GCTCAGC GC^T[ACGT]AGC
BsaI 583 GAGACC GGTCTC(1/5)
BspMI 506 ACCTGC ACCTGC(4/8)
BsrBI 796 GAGCGG CCGCTC(-3/-3)
BssHII 178 GCGCGC G^CGCGC
BstEII 1056 GGTCACC G^GT[ACGT]ACC
BstPI 1056 GGTCACC G^GT[ACGT]ACC
Bsu36I 927 CCTGAGG CC^T[ACGT]AGG
CelII 1227 GCTCAGC GC^T[ACGT]AGC
DrdI 487 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 256 CTCTTC CTCTTC(1/4)
Eco47III 1187 AGCGCT AGC^GCT
Eco81I 927 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1056 GGTCACC G^GT[ACGT]ACC
HinfI 358 GAGTC G^A[ACGT]TC
KasI 223 GGCGCC G^GCGCC
Ksp632I 256 CTCTTC CTCTTC(1/4)
KspI 121 CCGCGG CCGC^GG
MseI 998 TTAA T^TAA
MslI 57 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 199 GCCGGC GCC^GGC
NarI 223 GGCGCC GG^CGCC
NcoI 884 CCATGG C^CATGG
NdeI 49 CATATG CA^TATG
NgoMIV 199 GCCGGC G^CCGGC
NotI 309 GCGGCCGC GC^GGCCGC
PleI 358 GAGTC GAGTC(4/5)
PmaCI 692 CACGTG CAC^GTG
PmlI 692 CACGTG CAC^GTG
PvuII 757 CAGCTG CAG^CTG
RsaI 825 GTAC GT^AC
SacI 1210 GAGCTC GAGCT^C
SacII 121 CCGCGG CCGC^GG
SapI 255 GCTCTTC GCTCTTC(1/4)
SfoI 223 GGCGCC GGC^GCC
SmlI 820 CTCAAG C^T[CT][GA]AG
StuI 843 AGGCCT AGG^CCT
Tru9I 998 TTAA T^TAA
Tsp509I 662 AATT ^AATT
XcmI 1040 CCAAGAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
ApaI 145 GGGCCC GGGCC^C
282 GGGCCC
AspHI 1210 GAGCTC G[AT]GC[AT]^C
1225 GTGCTC
BalI 53 TGGCCA TGG^CCA
1037 TGGCCA
BanI 196 GGTGCC G^G[CT][GA]CC
223 GGCGCC
BbsI 613 GAAGAC GAAGAC(2/6)
778 GAAGAC
BglI 520 GCCAGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
871 GCCCACCTGGC
BpuAI 613 GAAGAC GAAGAC(2/6)
778 GAAGAC
BsaAI 692 CACGTG [CT]AC^GT[GA]
913 TACGTG
BseRI 793 GAGGAG GAGGAG(10/8)
1003 GAGGAG
BsiEI 201 CGGCCG CG[GA][CT]^CG
310 CGGCCG
BsiHKAI 1210 GAGCTC G[AT]GC[AT]^C
1225 GTGCTC
BsmAI 583 GAGAC GTCTC(1/5)
688 GAGAC
BsrI 1148 ACTGG ACTGG(1/-1)
521 CCAGT
BstAPI 504 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
840 GCAAGGCCTGC
BstYI 95 AGATCT [GA]^GATC[CT]
638 GGATCT
DsaI 121 CCGCGG C^C[GA][CT]GG
884 CCATGG
EagI 201 CGGCCG C^GGCCG
310 CGGCCG
EclXI 201 CGGCCG C^GGCCG
310 CGGCCG
Eco52I 201 CGGCCG C^GGCCG
310 CGGCCG
MflI 95 AGATCT [GA]^GATC[CT]
638 GGATCT
MluNI 53 TGGCCA TGG^CCA
1037 TGGCCA
MscI 53 TGGCCA TGG^CCA
1037 TGGCCA
PpuMI 294 GGGTCCC [GA]G^G[AT]CC[CT]
1031 GGGACCT
SmaI 279 CCCGGG CCC^GGG
291 CCCGGG
TaqI 59 TCGA T^CGA
1184 TCGA
Tsp45I 650 GTGAC ^GT[GC]AC
1057 GTCAC
TthHB8I 59 TCGA T^CGA
1184 TCGA
XhoII 95 AGATCT [GA]^GATC[CT]
638 GGATCT
XmaCI 279 CCCGGG C^CCGGG
291 CCCGGG
XmaI 279 CCCGGG C^CCGGG
291 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI