################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbvCI BciVI BclI BfaI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MunI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 661 AAATTC [GA]^AATT[CT] AfaI 825 GTAC GT^AC AlwNI 1217 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 927 CCTGAGG CC^T[ACGT]AGG Aor51HI 1187 AGCGCT AGC^GCT ApoI 661 AAATTC [GA]^AATT[CT] BbeI 223 GGCGCC GGCGC^C BbrPI 692 CACGTG CAC^GTG BcgI 366 CGACTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 95 AGATCT A^GATCT BlpI 1227 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1227 GCTCAGC GC^T[ACGT]AGC BsaI 583 GAGACC GGTCTC(1/5) BspMI 506 ACCTGC ACCTGC(4/8) BsrBI 796 GAGCGG CCGCTC(-3/-3) BssHII 178 GCGCGC G^CGCGC BstEII 1056 GGTCACC G^GT[ACGT]ACC BstPI 1056 GGTCACC G^GT[ACGT]ACC Bsu36I 927 CCTGAGG CC^T[ACGT]AGG CelII 1227 GCTCAGC GC^T[ACGT]AGC DrdI 487 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 256 CTCTTC CTCTTC(1/4) Eco47III 1187 AGCGCT AGC^GCT Eco81I 927 CCTGAGG CC^T[ACGT]AGG EcoO65I 1056 GGTCACC G^GT[ACGT]ACC HinfI 358 GAGTC G^A[ACGT]TC KasI 223 GGCGCC G^GCGCC Ksp632I 256 CTCTTC CTCTTC(1/4) KspI 121 CCGCGG CCGC^GG MseI 998 TTAA T^TAA MslI 57 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 199 GCCGGC GCC^GGC NarI 223 GGCGCC GG^CGCC NcoI 884 CCATGG C^CATGG NdeI 49 CATATG CA^TATG NgoMIV 199 GCCGGC G^CCGGC NotI 309 GCGGCCGC GC^GGCCGC PleI 358 GAGTC GAGTC(4/5) PmaCI 692 CACGTG CAC^GTG PmlI 692 CACGTG CAC^GTG PvuII 757 CAGCTG CAG^CTG RsaI 825 GTAC GT^AC SacI 1210 GAGCTC GAGCT^C SacII 121 CCGCGG CCGC^GG SapI 255 GCTCTTC GCTCTTC(1/4) SfoI 223 GGCGCC GGC^GCC SmlI 820 CTCAAG C^T[CT][GA]AG StuI 843 AGGCCT AGG^CCT Tru9I 998 TTAA T^TAA Tsp509I 662 AATT ^AATT XcmI 1040 CCAAGAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### ApaI 145 GGGCCC GGGCC^C 282 GGGCCC AspHI 1210 GAGCTC G[AT]GC[AT]^C 1225 GTGCTC BalI 53 TGGCCA TGG^CCA 1037 TGGCCA BanI 196 GGTGCC G^G[CT][GA]CC 223 GGCGCC BbsI 613 GAAGAC GAAGAC(2/6) 778 GAAGAC BglI 520 GCCAGTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 871 GCCCACCTGGC BpuAI 613 GAAGAC GAAGAC(2/6) 778 GAAGAC BsaAI 692 CACGTG [CT]AC^GT[GA] 913 TACGTG BseRI 793 GAGGAG GAGGAG(10/8) 1003 GAGGAG BsiEI 201 CGGCCG CG[GA][CT]^CG 310 CGGCCG BsiHKAI 1210 GAGCTC G[AT]GC[AT]^C 1225 GTGCTC BsmAI 583 GAGAC GTCTC(1/5) 688 GAGAC BsrI 1148 ACTGG ACTGG(1/-1) 521 CCAGT BstAPI 504 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 840 GCAAGGCCTGC BstYI 95 AGATCT [GA]^GATC[CT] 638 GGATCT DsaI 121 CCGCGG C^C[GA][CT]GG 884 CCATGG EagI 201 CGGCCG C^GGCCG 310 CGGCCG EclXI 201 CGGCCG C^GGCCG 310 CGGCCG Eco52I 201 CGGCCG C^GGCCG 310 CGGCCG MflI 95 AGATCT [GA]^GATC[CT] 638 GGATCT MluNI 53 TGGCCA TGG^CCA 1037 TGGCCA MscI 53 TGGCCA TGG^CCA 1037 TGGCCA PpuMI 294 GGGTCCC [GA]G^G[AT]CC[CT] 1031 GGGACCT SmaI 279 CCCGGG CCC^GGG 291 CCCGGG TaqI 59 TCGA T^CGA 1184 TCGA Tsp45I 650 GTGAC ^GT[GC]AC 1057 GTCAC TthHB8I 59 TCGA T^CGA 1184 TCGA XhoII 95 AGATCT [GA]^GATC[CT] 638 GGATCT XmaCI 279 CCCGGG C^CCGGG 291 CCCGGG XmaI 279 CCCGGG C^CCGGG 291 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AflIII AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI