Restriction map for
pFN21AE0293
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BciVI BclI BfaI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I Eco47III
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 568 GTCTAC GT^[AC][GT]AC
AcyI 295 GGCGCC G[GA]^CG[CT]C
AlwI 533 GGATC GGATC(4/5)
AlwNI 576 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 550 GGGCCC GGGCC^C
AspHI 706 GTGCTC G[AT]GC[AT]^C
AvaI 66 CCCGAG C^[CT]CG[GA]G
BalI 234 TGGCCA TGG^CCA
BbeI 295 GGCGCC GGCGC^C
BbvCI 824 CCTCAGC CCTCAGC(-5/-2)
BcgI 716 GCAGCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 295 GGCGCC G[GA]^CG[CT]C
BseRI 768 CTCCTC GAGGAG(10/8)
BsgI 389 GTGCAG GTGCAG(16/14)
BsiHKAI 706 GTGCTC G[AT]GC[AT]^C
BsmAI 901 GTCTC GTCTC(1/5)
BsmBI 900 CGTCTC CGTCTC(1/5)
BsoBI 66 CCCGAG C^[CT]CG[GA]G
BsrDI 223 GCAATG GCAATG(2/0)
BsrFI 540 GCCGGC [GA]^CCGG[CT]
BstEII 282 GGTAACC G^GT[ACGT]ACC
BstPI 282 GGTAACC G^GT[ACGT]ACC
Cfr10I 540 GCCGGC [GA]^CCGG[CT]
DpnII 534 GATC ^GATC
EagI 44 CGGCCG C^GGCCG
EarI 835 CTCTTC CTCTTC(1/4)
EclXI 44 CGGCCG C^GGCCG
Eco52I 44 CGGCCG C^GGCCG
EcoNI 918 CCTTATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 282 GGTAACC G^GT[ACGT]ACC
FauI 73 CCCGC CCCGC(4/6)
Hin1I 295 GGCGCC G[GA]^CG[CT]C
KasI 295 GGCGCC G^GCGCC
Ksp632I 835 CTCTTC CTCTTC(1/4)
MboI 534 GATC ^GATC
MluNI 234 TGGCCA TGG^CCA
MscI 234 TGGCCA TGG^CCA
MseI 190 TTAA T^TAA
MslI 132 CACGTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 540 GCCGGC GCC^GGC
NarI 295 GGCGCC GG^CGCC
NcoI 546 CCATGG C^CATGG
NdeII 534 GATC ^GATC
NgoMIV 540 GCCGGC G^CCGGC
PvuII 11 CAGCTG CAG^CTG
Sau3AI 534 GATC ^GATC
SfoI 295 GGCGCC GGC^GCC
TfiI 890 GATTC G^A[AT]TC
Tru9I 190 TTAA T^TAA
Tsp45I 436 GTGAC ^GT[GC]AC
Tsp509I 943 AATT ^AATT
TspRI 476 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 554 CCACCAACATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 59 CGCG CG^CG
537 CGCG
BanI 295 GGCGCC G^G[CT][GA]CC
374 GGTGCC
BcnI 47 CCGGG CC^[GC]GG
840 CCGGG
BlpI 381 GCTAAGC GC^T[ACGT]AGC
448 GCTGAGC
BpmI 454 CTGGAG CTGGAG(16/14)
754 CTGGAG
Bpu1102I 381 GCTAAGC GC^T[ACGT]AGC
448 GCTGAGC
BsiEI 44 CGGCCG CG[GA][CT]^CG
896 CGACCG
BsmFI 4 GGGAC GGGAC(10/14)
268 GTCCC
BstF5I 831 CATCC GGATG(2/0)
878 GGATG
BstUI 59 CGCG CG^CG
537 CGCG
BstXI 272 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
557 CCAACATCATGG
CelII 381 GCTAAGC GC^T[ACGT]AGC
448 GCTGAGC
FokI 831 CATCC GGATG(9/13)
878 GGATG
HaeII 295 GGCGCC [GA]GCGC^[CT]
750 GGCGCT
HphI 120 GGTGA GGTGA(8/7)
602 TCACC
MaeIII 283 GTAAC ^GT[ACGT]AC
436 GTGAC
MspA1I 11 CAGCTG C[AC]G^C[GT]G
20 CAGCGG
MvnI 59 CGCG CG^CG
537 CGCG
NciI 47 CCGGG CC^[GC]GG
840 CCGGG
PleI 441 GACTC GAGTC(4/5)
766 GACTC
PstI 8 CTGCAG CTGCA^G
714 CTGCAG
TaqI 112 TCGA T^CGA
764 TCGA
TthHB8I 112 TCGA T^CGA
764 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboII MnlI MspI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI StyI TseI