Restriction map for
pFN21AB7182
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstF5I BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI Eam1105I
EarI Eco47III Eco81I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MaeII MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NdeII NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AlwNI 422 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 10 GGGCCC GGGCC^C
AspI 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 223 CCCGGG C^[CT]CG[GA]G
AviII 113 TGCGCA TGC^GCA
BalI 471 TGGCCA TGG^CCA
BanI 238 GGCGCC G^G[CT][GA]CC
BbeI 238 GGCGCC GGCGC^C
BbsI 448 GAAGAC GAAGAC(2/6)
BpmI 300 CTGGAG CTGGAG(16/14)
BpuAI 448 GAAGAC GAAGAC(2/6)
BseRI 437 CTCCTC GAGGAG(10/8)
BsoBI 223 CCCGGG C^[CT]CG[GA]G
BspMI 77 ACCTGC ACCTGC(4/8)
BstEII 519 GGTGACC G^GT[ACGT]ACC
BstPI 519 GGTGACC G^GT[ACGT]ACC
DdeI 542 CTGAG C^T[ACGT]AG
DraIII 91 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 76 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 121 CCGCGG C^C[GA][CT]GG
EcoNI 378 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 519 GGTGACC G^GT[ACGT]ACC
FseI 534 GGCCGGCC GGCCGG^CC
FspI 113 TGCGCA TGC^GCA
KasI 238 GGCGCC G^GCGCC
KspI 121 CCGCGG CCGC^GG
MaeIII 520 GTGAC ^GT[ACGT]AC
MboII 448 GAAGA GAAGA(8/7)
MluNI 471 TGGCCA TGG^CCA
MscI 471 TGGCCA TGG^CCA
NarI 238 GGCGCC GG^CGCC
PflFI 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 90 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 181 GAGTC GAGTC(4/5)
PstI 169 CTGCAG CTGCA^G
SacII 121 CCGCGG CCGC^GG
SfaNI 415 GATGC GCATC(5/9)
SfcI 169 CTGCAG C^T[GA][CT]AG
SfoI 238 GGCGCC GGC^GCC
SmaI 223 CCCGGG CCC^GGG
TaqI 74 TCGA T^CGA
TfiI 407 GAATC G^A[AT]TC
Tsp45I 520 GTGAC ^GT[GC]AC
Tth111I 382 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 74 TCGA T^CGA
Van91I 90 CCACTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 223 CCCGGG C^CCGGG
XmaI 223 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcyI 238 GGCGCC G[GA]^CG[CT]C
364 GACGCC
BglI 20 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
199 GCCGGCGAGGC
BsaHI 238 GGCGCC G[GA]^CG[CT]C
364 GACGCC
BsmAI 356 GAGAC GTCTC(1/5)
399 GTCTC
BsrI 264 ACTGG ACTGG(1/-1)
516 ACTGG
BstXI 494 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
509 CCACTGAACTGG
Hin1I 238 GGCGCC G[GA]^CG[CT]C
364 GACGCC
HinfI 181 GAGTC G^A[ACGT]TC
407 GAATC
HphI 294 GGTGA GGTGA(8/7)
519 GGTGA
MspA1I 121 CCGCGG C[AC]G^C[GT]G
313 CAGCGG
NaeI 199 GCCGGC GCC^GGC
535 GCCGGC
NgoMIV 199 GCCGGC G^CCGGC
535 GCCGGC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiEI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DraII EaeI EagI
EclXI Eco52I EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI TspRI