Restriction map for
pFN21AE0590
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BciVI BfrI BglII
BlnI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
ClaI CpoI DraI DraIII DrdI
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 383 GAATTT [GA]^AATT[CT]
AflIII 1196 ACATGT A^C[GA][CT]GT
AhdI 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 67 GTGCAC G^TGCAC
AlwI 459 GATCC GGATC(4/5)
AlwNI 550 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1165 CCTGAGG CC^T[ACGT]AGG
ApaLI 67 GTGCAC G^TGCAC
ApoI 383 GAATTT [GA]^AATT[CT]
AspEI 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 583 CACGTG CAC^GTG
BbsI 265 GTCTTC GAAGAC(2/6)
BbvCI 123 CCTCAGC CCTCAGC(-5/-2)
BcgI 706 GCAGACAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 860 TGATCA T^GATCA
BfaI 1276 CTAG C^TAG
BlpI 144 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 144 GCTCAGC GC^T[ACGT]AGC
BpuAI 265 GTCTTC GAAGAC(2/6)
BspLU11I 1196 ACATGT A^CATGT
BspMI 1160 ACCTGC ACCTGC(4/8)
BsrBI 212 GAGCGG CCGCTC(-3/-3)
BsrFI 800 ACCGGC [GA]^CCGG[CT]
BsrI 424 ACTGG ACTGG(1/-1)
BssHII 137 GCGCGC G^CGCGC
Bsu36I 1165 CCTGAGG CC^T[ACGT]AGG
CelII 144 GCTCAGC GC^T[ACGT]AGC
Cfr10I 800 ACCGGC [GA]^CCGG[CT]
EagI 194 CGGCCG C^GGCCG
Eam1105I 1012 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 194 CGGCCG C^GGCCG
Eco52I 194 CGGCCG C^GGCCG
Eco81I 1165 CCTGAGG CC^T[ACGT]AGG
EcoNI 1161 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 816 CCAAGG C^C[AT][AT]GG
FbaI 860 TGATCA T^GATCA
MaeI 1276 CTAG C^TAG
MaeIII 40 GTGAC ^GT[ACGT]AC
MseI 968 TTAA T^TAA
MslI 605 CACATTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 583 CACGTG CAC^GTG
PmlI 583 CACGTG CAC^GTG
PpuMI 638 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 117 CTGCAG CTGCA^G
PvuII 1036 CAGCTG CAG^CTG
SacI 940 GAGCTC GAGCT^C
ScaI 734 AGTACT AGT^ACT
SfcI 117 CTGCAG C^T[GA][CT]AG
SspI 405 AATATT AAT^ATT
StuI 1256 AGGCCT AGG^CCT
StyI 816 CCAAGG C^C[AT][AT]GG
Tru9I 968 TTAA T^TAA
Tsp45I 40 GTGAC ^GT[GC]AC
Tsp509I 384 AATT ^AATT
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 526 GGGCCC GGGCC^C
975 GGGCCC
Asp700I 501 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1266 GAATGACTTC
AspHI 67 GTGCAC G[AT]GC[AT]^C
940 GAGCTC
BsaAI 583 CACGTG [CT]AC^GT[GA]
1216 TACGTG
BsaI 103 GGTCTC GGTCTC(1/5)
578 GGTCTC
BsaWI 656 ACCGGA [AT]^CCGG[AT]
1293 ACCGGA
BsgI 438 GTGCAG GTGCAG(16/14)
704 GTGCAG
BsiEI 194 CGGCCG CG[GA][CT]^CG
787 CGGTCG
BsiHKAI 67 GTGCAC G[AT]GC[AT]^C
940 GAGCTC
BsrDI 519 CATTGC GCAATG(2/0)
1065 CATTGC
KspI 84 CCGCGG CCGC^GG
244 CCGCGG
NspI 317 ACATGC [GA]CATG^[CT]
1196 ACATGT
SacII 84 CCGCGG CCGC^GG
244 CCGCGG
SmaI 635 CCCGGG CCC^GGG
1205 CCCGGG
TaqI 413 TCGA T^CGA
686 TCGA
TthHB8I 413 TCGA T^CGA
686 TCGA
XcmI 926 CCAAGCCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1211 CCAAGTACGTGATGG
XmaCI 635 CCCGGG C^CCGGG
1205 CCCGGG
XmaI 635 CCCGGG C^CCGGG
1205 CCCGGG
XmnI 501 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1266 GAATGACTTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI TfiI
TseI TspRI