Restriction map for
pFN21AA0002
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccIII AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1174 GTAGAC GT^[AC][GT]AC
AccII 1348 CGCG CG^CG
AciI 1347 CCGC CCGC(-3/-1)
AclI 1429 AACGTT AA^CGTT
AcyI 1108 GGCGCC G[GA]^CG[CT]C
AflIII 1285 ACATGT A^C[GA][CT]GT
AlwNI 1544 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1594 GGGCCC GGGCC^C
AspHI 326 GAGCTC G[AT]GC[AT]^C
AvaII 142 GGACC G^G[AT]CC
BalI 536 TGGCCA TGG^CCA
BanI 1108 GGCGCC G^G[CT][GA]CC
BbeI 1108 GGCGCC GGCGC^C
BbsI 965 GAAGAC GAAGAC(2/6)
BbvCI 1324 GCTGAGG CCTCAGC(-5/-2)
BpuAI 965 GAAGAC GAAGAC(2/6)
BsaHI 1108 GGCGCC G[GA]^CG[CT]C
BsiHKAI 326 GAGCTC G[AT]GC[AT]^C
BspHI 417 TCATGA T^CATGA
BspLU11I 1285 ACATGT A^CATGT
BstUI 1348 CGCG CG^CG
BstYI 957 GGATCT [GA]^GATC[CT]
EcoT14I 17 CCAAGG C^C[AT][AT]GG
FauI 1346 CCCGC CCCGC(4/6)
Hin1I 1108 GGCGCC G[GA]^CG[CT]C
HphI 685 GGTGA GGTGA(8/7)
KasI 1108 GGCGCC G^GCGCC
MflI 957 GGATCT [GA]^GATC[CT]
MluNI 536 TGGCCA TGG^CCA
MscI 536 TGGCCA TGG^CCA
MspA1I 1586 CAGCTG C[AC]G^C[GT]G
MvnI 1348 CGCG CG^CG
NarI 1108 GGCGCC GG^CGCC
PflMI 1585 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1429 AACGTT AA^CGTT
PvuII 1586 CAGCTG CAG^CTG
RcaI 417 TCATGA T^CATGA
SacI 326 GAGCTC GAGCT^C
ScaI 1125 AGTACT AGT^ACT
SfoI 1108 GGCGCC GGC^GCC
SmlI 43 CTCAAG C^T[CT][GA]AG
SphI 556 GCATGC GCATG^C
SspI 1511 AATATT AAT^ATT
StyI 17 CCAAGG C^C[AT][AT]GG
Van91I 1585 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1491 TCTAGA T^CTAGA
XhoII 957 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 617 AAATTC [GA]^AATT[CT]
798 GAATTT
AlwI 957 GGATC GGATC(4/5)
1563 GGATC
ApoI 617 AAATTC [GA]^AATT[CT]
798 GAATTT
BcnI 25 CCGGG CC^[GC]GG
1228 CCCGG
BpmI 323 CTGGAG CTGGAG(16/14)
1367 CTGGAG
BsaJI 17 CCAAGG C^C[ACGT][ACGT]GG
1503 CCTGGG
BsmAI 1282 GAGAC GTCTC(1/5)
478 GTCTC
BsmFI 1032 GGGAC GGGAC(10/14)
1456 GTCCC
EaeI 536 TGGCCA [CT]^GGCC[GA]
582 CGGCCA
HaeII 3 GGCGCT [GA]GCGC^[CT]
1108 GGCGCC
HindIII 101 AAGCTT A^AGCTT
541 AAGCTT
HinfI 423 GATTC G^A[ACGT]TC
577 GATTC
MslI 586 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
930 CATGTTAGTG
NciI 25 CCGGG CC^[GC]GG
1228 CCCGG
PstI 405 CTGCAG CTGCA^G
1541 CTGCAG
SapI 901 GCTCTTC GCTCTTC(1/4)
994 GCTCTTC
TaqI 462 TCGA T^CGA
1131 TCGA
TfiI 423 GATTC G^A[AT]TC
577 GATTC
TthHB8I 462 TCGA T^CGA
1131 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AfaI AluI Asp700I BanII BbvI
BfaI BmyI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI