Restriction map for
pFN21AB4605
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbsI
BcgI BclI BglI BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsiYI BslI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MaeII MamI MluI MroI MslI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 47 CGCG CG^CG
AcyI 84 GACGCC G[GA]^CG[CT]C
AflII 820 CTTAAG C^TTAAG
AflIII 384 ACATGT A^C[GA][CT]GT
Alw44I 1299 GTGCAC G^TGCAC
ApaLI 1299 GTGCAC G^TGCAC
AvaII 356 GGACC G^G[AT]CC
BalI 408 TGGCCA TGG^CCA
BamHI 1225 GGATCC G^GATCC
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
BciVI 845 GTATCC GTATCC(6/5)
BfrI 820 CTTAAG C^TTAAG
BlpI 676 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 676 GCTCAGC GC^T[ACGT]AGC
BsaHI 84 GACGCC G[GA]^CG[CT]C
BseRI 1382 GAGGAG GAGGAG(10/8)
BsmFI 355 GGGAC GGGAC(10/14)
BspHI 536 TCATGA T^CATGA
BspLU11I 384 ACATGT A^CATGT
BstUI 47 CGCG CG^CG
Cac8I 4 GCGGGC GC[ACGT]^[ACGT]GC
CelII 676 GCTCAGC GC^T[ACGT]AGC
DraIII 1035 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 4 GCGGG CCCGC(4/6)
Hin1I 84 GACGCC G[GA]^CG[CT]C
MfeI 137 CAATTG C^AATTG
MluNI 408 TGGCCA TGG^CCA
MscI 408 TGGCCA TGG^CCA
MspA1I 679 CAGCTG C[AC]G^C[GT]G
MunI 137 CAATTG C^AATTG
MvnI 47 CGCG CG^CG
NspI 384 ACATGT [GA]CATG^[CT]
PleI 24 GACTC GAGTC(4/5)
PvuII 679 CAGCTG CAG^CTG
RcaI 536 TCATGA T^CATGA
SacI 653 GAGCTC GAGCT^C
SapI 463 GCTCTTC GCTCTTC(1/4)
ScaI 1068 AGTACT AGT^ACT
SexAI 891 ACCTGGT A^CC[AT]GGT
SfcI 527 CTATAG C^T[GA][CT]AG
SmlI 820 CTTAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 725 AAATTC [GA]^AATT[CT]
1115 AAATTT
ApoI 725 AAATTC [GA]^AATT[CT]
1115 AAATTT
BanII 653 GAGCTC G[GA]GC[CT]^C
757 GAGCCC
BcnI 761 CCCGG CC^[GC]GG
1044 CCGGG
BglII 307 AGATCT A^GATCT
778 AGATCT
BsgI 443 GTGCAG GTGCAG(16/14)
1076 GTGCAG
BsmAI 743 GAGAC GTCTC(1/5)
1163 GAGAC
BstF5I 69 GGATG GGATG(2/0)
456 GGATG
Cfr13I 356 GGACC G^G[ACGT]CC
870 GGGCC
EaeI 292 CGGCCA [CT]^GGCC[GA]
408 TGGCCA
EcoT14I 65 CCTTGG C^C[AT][AT]GG
633 CCAAGG
FokI 69 GGATG GGATG(9/13)
456 GGATG
HapII 762 CCGG C^CGG
1044 CCGG
HgaI 84 GACGC GACGC(5/10)
48 GCGTC
HindIII 238 AAGCTT A^AGCTT
268 AAGCTT
HpaII 762 CCGG C^CGG
1044 CCGG
HphI 1215 GGTGA GGTGA(8/7)
59 TCACC
MspI 762 CCGG C^CGG
1044 CCGG
NciI 761 CCCGG CC^[GC]GG
1044 CCGGG
NlaIV 355 GGGACC GG[ACGT]^[ACGT]CC
1225 GGATCC
Sau96I 356 GGACC G^G[ACGT]CC
870 GGGCC
SfaNI 457 GATGC GCATC(5/9)
39 GCATC
SspI 226 AATATT AAT^ATT
691 AATATT
StyI 65 CCTTGG C^C[AT][AT]GG
633 CCAAGG
TfiI 487 GAATC G^A[AT]TC
558 GATTC
Tsp45I 22 GTGAC ^GT[GC]AC
1387 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BbvI BfaI BmyI BpmI BsaJI
BsiHKAI Bsp1286I BsrI BssKI BstNI
BstYI DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HincII HindII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI Tru9I TseI Tsp509I TspRI
XhoII