Restriction map for
pFN21AE0189
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MslI MspI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 340 GAATTT [GA]^AATT[CT]
AlwNI 602 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 340 GAATTT [GA]^AATT[CT]
AspI 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 247 GGACC G^G[AT]CC
BcgI 132 GCATGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 486 AGATCT A^GATCT
BpmI 69 CTCCAG CTGGAG(16/14)
BseRI 619 GAGGAG GAGGAG(10/8)
BsiYI 540 CCGTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 540 CCGTTTTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrDI 158 GCAATG GCAATG(2/0)
BstYI 486 AGATCT [GA]^GATC[CT]
Cac8I 198 GCCAGC GC[ACGT]^[ACGT]GC
DraI 343 TTTAAA TTT^AAA
EarI 571 CTCTTC CTCTTC(1/4)
Ksp632I 571 CTCTTC CTCTTC(1/4)
MaeII 348 ACGT A^CGT
MflI 486 AGATCT [GA]^GATC[CT]
MseI 344 TTAA T^TAA
MspA1I 602 CAGCTG C[AC]G^C[GT]G
PflFI 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 138 GACTC GAGTC(4/5)
PvuII 602 CAGCTG CAG^CTG
SmlI 378 CTCAAG C^T[CT][GA]AG
TaqI 78 TCGA T^CGA
Tru9I 344 TTAA T^TAA
TspRI 84 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 248 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 78 TCGA T^CGA
XcmI 114 CCAGAGCTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 486 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 27 GTAC GT^AC
371 GTAC
AlwI 205 GGATC GGATC(4/5)
524 GGATC
BsmAI 136 GAGAC GTCTC(1/5)
149 GAGAC
Cfr13I 247 GGACC G^G[ACGT]CC
628 GGGCC
HaeIII 538 GGCC GG^CC
629 GGCC
MaeIII 407 GTTAC ^GT[ACGT]AC
413 GTAAC
NlaIII 133 CATG CATG^
226 CATG
NlaIV 247 GGACCC GG[ACGT]^[ACGT]CC
627 GGGGCC
PstI 331 CTGCAG CTGCA^G
585 CTGCAG
RsaI 27 GTAC GT^AC
371 GTAC
Sau96I 247 GGACC G^G[ACGT]CC
628 GGGCC
SfaNI 235 GATGC GCATC(5/9)
361 GATGC
SfcI 331 CTGCAG C^T[GA][CT]AG
585 CTGCAG
TfiI 80 GATTC G^A[AT]TC
222 GAATC
Tsp509I 341 AATT ^AATT
355 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsrI DdeI DpnII
Fnu4HI HinfI ItaI MboI MboII
MnlI MwoI NdeII Sau3AI TseI