##################################
# NO CUT ENZYMES (  204 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeIII      HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TaqI        TfiI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwI          271  GGATC                GGATC(4/5)
AlwNI         475  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       142  AGCGCT               AGC^GCT
Asp700I       289  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI         411  GGATAC               GTATCC(6/5)
BglII         200  AGATCT               A^GATCT
BsmAI          20  GAGAC                GTCTC(1/5)
BsmBI          20  GAGACG               CGTCTC(1/5)
BstF5I        392  GGATG                GGATG(2/0)
BstYI         200  AGATCT               [GA]^GATC[CT]
Cac8I          45  GCGAGC               GC[ACGT]^[ACGT]GC
CfoI          143  GCGC                 GCG^C
DraII          63  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eco47III      142  AGCGCT               AGC^GCT
EcoO109I       63  AGGACCT              [GA]G^G[ACGT]CC[CT]
FauI           16  GCGGG                CCCGC(4/6)
FokI          392  GGATG                GGATG(9/13)
HaeII         142  AGCGCT               [GA]GCGC^[CT]
HapII          59  CCGG                 C^CGG
HhaI          143  GCGC                 GCG^C
HinP1I        143  GCGC                 G^CGC
HpaII          59  CCGG                 C^CGG
MflI          200  AGATCT               [GA]^GATC[CT]
MseI          338  TTAA                 T^TAA
MspI           59  CCGG                 C^CGG
NlaIV          52  GGAGCC               GG[ACGT]^[ACGT]CC
PpuMI          63  AGGACCT              [GA]G^G[AT]CC[CT]
SfaNI         247  GCATC                GCATC(5/9)
SspI          400  AATATT               AAT^ATT
Tru9I         338  TTAA                 T^TAA
XhoII         200  AGATCT               [GA]^GATC[CT]
XmnI          289  GAAACTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AvaII          64  GGACC                G^G[AT]CC
              430  GGACC               
BpmI          374  CTCCAG               CTGGAG(16/14)
               50  CTGGAG              
BsrI          113  CCAGT                ACTGG(1/-1)
              134  CCAGT               
Cfr13I         64  GGACC                G^G[ACGT]CC
              430  GGACC               
DdeI           98  CTAAG                C^T[ACGT]AG
              146  CTGAG               
MspA1I        158  CAGCTG               C[AC]G^C[GT]G
              425  CAGCTG              
MwoI           49  GCTGGAGCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              480  GCTGTGGGAGC         
NlaIII        457  CATG                 CATG^
              520  CATG                
PvuII         158  CAGCTG               CAG^CTG
              425  CAGCTG              
Sau96I         64  GGACC                G^G[ACGT]CC
              430  GGACC               


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AluI        BbvI        DpnII       Fnu4HI      
ItaI        MboI        MboII       MnlI        NdeII       
Sau3AI      TseI