Restriction map for
pFN21AB7308
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstUI BstXI CfoI Cfr10I CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoNI FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 1379 GGTACC G^GTACC
AciI 937 GCGG CCGC(-3/-1)
AclI 930 AACGTT AA^CGTT
AhdI 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 763 GATCC GGATC(4/5)
AlwNI 1102 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 426 ATTAAT AT^TAAT
AsnI 426 ATTAAT AT^TAAT
Asp718I 1379 GGTACC G^GTACC
AspEI 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2437 GAGCTC G[AT]GC[AT]^C
AvaI 2023 CTCGAG C^[CT]CG[GA]G
BanI 1379 GGTACC G^G[CT][GA]CC
BbsI 255 GAAGAC GAAGAC(2/6)
BglII 1785 AGATCT A^GATCT
BlpI 1059 GCTAAGC GC^T[ACGT]AGC
BpmI 1190 CTCCAG CTGGAG(16/14)
Bpu1102I 1059 GCTAAGC GC^T[ACGT]AGC
BpuAI 255 GAAGAC GAAGAC(2/6)
BsaBI 1341 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1310 CTGCAC GTGCAG(16/14)
BsiHKAI 2437 GAGCTC G[AT]GC[AT]^C
BsmFI 2048 GGGAC GGGAC(10/14)
BsoBI 2023 CTCGAG C^[CT]CG[GA]G
BspDI 1541 ATCGAT AT^CGAT
BspMI 324 GCAGGT ACCTGC(4/8)
Bst1107I 2039 GTATAC GTA^TAC
BstAPI 1155 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 381 GGTTACC G^GT[ACGT]ACC
BstPI 381 GGTTACC G^GT[ACGT]ACC
BstZ17I 2039 GTATAC GTA^TAC
Cac8I 2301 GCAAGC GC[ACGT]^[ACGT]GC
CelII 1059 GCTAAGC GC^T[ACGT]AGC
ClaI 1541 ATCGAT AT^CGAT
DraI 969 TTTAAA TTT^AAA
DraII 2048 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1383 CCATGG C^C[GA][CT]GG
Eam1105I 1889 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2048 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 381 GGTTACC G^GT[ACGT]ACC
EcoRV 601 GATATC GAT^ATC
EcoT22I 125 ATGCAT ATGCA^T
HgaI 1999 GACGC GACGC(5/10)
HindIII 467 AAGCTT A^AGCTT
KpnI 1379 GGTACC GGTAC^C
MamI 1341 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1280 CAATTG C^AATTG
MspA1I 1105 CAGCTG C[AC]G^C[GT]G
MunI 1280 CAATTG C^AATTG
NcoI 1383 CCATGG C^CATGG
NdeI 535 CATATG CA^TATG
NsiI 125 ATGCAT ATGCA^T
PaeR7I 2023 CTCGAG C^TCGAG
PpuMI 2048 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 426 ATTAAT AT^TAAT
Psp1406I 930 AACGTT AA^CGTT
PvuII 1105 CAGCTG CAG^CTG
SacI 2437 GAGCTC GAGCT^C
SapI 1319 GCTCTTC GCTCTTC(1/4)
ScaI 169 AGTACT AGT^ACT
SexAI 1464 ACCTGGT A^CC[AT]GGT
SfcI 417 CTATAG C^T[GA][CT]AG
SspI 1855 AATATT AAT^ATT
XcmI 386 CCAAAACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2023 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1897 GTCTAC GT^[AC][GT]AC
2039 GTATAC
AocI 1651 CCTAAGG CC^T[ACGT]AGG
1792 CCTCAGG
AvaII 283 GGACC G^G[AT]CC
2049 GGACC
BanII 1317 GGGCTC G[GA]GC[CT]^C
2437 GAGCTC
BciVI 2326 GGATAC GTATCC(6/5)
199 GTATCC
BclI 374 TGATCA T^GATCA
1845 TGATCA
BfaI 767 CTAG C^TAG
1150 CTAG
BsaI 2254 GAGACC GGTCTC(1/5)
1298 GGTCTC
BsaWI 2184 ACCGGA [AT]^CCGG[AT]
2330 ACCGGA
BseRI 979 GAGGAG GAGGAG(10/8)
1503 GAGGAG
BspHI 1677 TCATGA T^CATGA
2375 TCATGA
BspLU11I 1535 ACATGT A^CATGT
1774 ACATGT
BstYI 762 AGATCC [GA]^GATC[CT]
1785 AGATCT
Bsu36I 1651 CCTAAGG CC^T[ACGT]AGG
1792 CCTCAGG
EarI 1320 CTCTTC CTCTTC(1/4)
1002 GAAGAG
Eco81I 1651 CCTAAGG CC^T[ACGT]AGG
1792 CCTCAGG
EcoRI 24 GAATTC G^AATTC
441 GAATTC
EcoT14I 500 CCAAGG C^C[AT][AT]GG
1383 CCATGG
FbaI 374 TGATCA T^GATCA
1845 TGATCA
HaeIII 96 GGCC GG^CC
498 GGCC
HapII 2185 CCGG C^CGG
2331 CCGG
HpaII 2185 CCGG C^CGG
2331 CCGG
Ksp632I 1320 CTCTTC CTCTTC(1/4)
1002 GAAGAG
MaeI 767 CTAG C^TAG
1150 CTAG
MflI 762 AGATCC [GA]^GATC[CT]
1785 AGATCT
MspI 2185 CCGG C^CGG
2331 CCGG
NlaIV 1379 GGTACC GG[ACGT]^[ACGT]CC
2048 GGGACC
PflMI 203 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2168 CCATTAAATGG
PleI 625 GACTC GAGTC(4/5)
1452 GAGTC
RcaI 1677 TCATGA T^CATGA
2375 TCATGA
StyI 500 CCAAGG C^C[AT][AT]GG
1383 CCATGG
Van91I 203 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2168 CCATTAAATGG
XhoII 762 AGATCC [GA]^GATC[CT]
1785 AGATCT
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
Asp700I BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI