Restriction map for
pFN21AB0477
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaHI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HpaI KasI KpnI MaeI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 845 AAATTT [GA]^AATT[CT]
AflIII 665 ACGTGT A^C[GA][CT]GT
AgeI 677 ACCGGT A^CCGGT
ApaI 353 GGGCCC GGGCC^C
ApoI 845 AAATTT [GA]^AATT[CT]
AviII 190 TGCGCA TGC^GCA
BanI 777 GGTGCC G^G[CT][GA]CC
BglI 355 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1125 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI 181 GAGCGG CCGCTC(-3/-3)
BsrFI 677 ACCGGT [GA]^CCGG[CT]
BssHII 142 GCGCGC G^CGCGC
BssSI 1426 CTCGTG CACGAG(-5/-1)
BstEII 1254 GGTCACC G^GT[ACGT]ACC
BstPI 1254 GGTCACC G^GT[ACGT]ACC
Cfr10I 677 ACCGGT [GA]^CCGG[CT]
EcoO65I 1254 GGTCACC G^GT[ACGT]ACC
FspI 190 TGCGCA TGC^GCA
HgaI 7 GCGTC GACGC(5/10)
HincII 727 GTCAAC GT[CT]^[GA]AC
HindII 727 GTCAAC GT[CT]^[GA]AC
HindIII 745 AAGCTT A^AGCTT
HinfI 39 GACTC G^A[ACGT]TC
KspI 792 CCGCGG CCGC^GG
MamI 1125 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 11 CCATGG C^CATGG
NspI 193 GCATGC [GA]CATG^[CT]
PaeR7I 557 CTCGAG C^TCGAG
PinAI 677 ACCGGT A^CCGGT
PleI 39 GACTC GAGTC(4/5)
SacII 792 CCGCGG CCGC^GG
SbfI 492 CCTGCAGG CCTGCA^GG
SphI 193 GCATGC GCATG^C
Sse8387I 492 CCTGCAGG CCTGCA^GG
StuI 1220 AGGCCT AGG^CCT
XhoI 557 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 308 TCCGGA T^CCGGA
434 TCCGGA
BalI 672 TGGCCA TGG^CCA
1536 TGGCCA
BbvCI 166 CCTCAGC CCTCAGC(-5/-2)
285 GCTGAGG
BglII 876 AGATCT A^GATCT
1130 AGATCT
BseAI 308 TCCGGA T^CCGGA
434 TCCGGA
BseRI 478 GAGGAG GAGGAG(10/8)
1105 GAGGAG
BsgI 1272 CTGCAC GTGCAG(16/14)
385 GTGCAG
BsmAI 578 GAGAC GTCTC(1/5)
994 GAGAC
BspEI 308 TCCGGA T^CCGGA
434 TCCGGA
BstXI 369 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
663 CCACGTGTCTGG
DraIII 931 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
1342 CACACGGTG
DrdI 529 GACGTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
949 GACCTGCCAGTC
EarI 202 GAAGAG CTCTTC(1/4)
322 GAAGAG
Ksp632I 202 GAAGAG CTCTTC(1/4)
322 GAAGAG
MboII 202 GAAGA GAAGA(8/7)
322 GAAGA
MluNI 672 TGGCCA TGG^CCA
1536 TGGCCA
MroI 308 TCCGGA T^CCGGA
434 TCCGGA
MscI 672 TGGCCA TGG^CCA
1536 TGGCCA
PflMI 930 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1179 CCAAGAACTGG
SacI 223 GAGCTC GAGCT^C
1089 GAGCTC
SfaNI 1227 GATGC GCATC(5/9)
71 GCATC
SmaI 59 CCCGGG CCC^GGG
1016 CCCGGG
SmlI 475 CTTGAG C^T[CT][GA]AG
557 CTCGAG
Tsp509I 621 AATT ^AATT
846 AATT
Van91I 930 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1179 CCAAGAACTGG
XmaCI 59 CCCGGG C^CCGGG
1016 CCCGGG
XmaI 59 CCCGGG C^CCGGG
1016 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbrPI BbvI BcnI BmyI BpmI
BsaAI BsaJI BsaWI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII