Restriction map for
pFN21AB6456
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI DsaI
Eam1105I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HpaI
KasI KpnI KspI MaeI MamI
MluI MluNI MroI MscI MspA1I
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 205 GTCGAC GT^[AC][GT]AC
AciI 25 GCGG CCGC(-3/-1)
Aor51HI 326 AGCGCT AGC^GCT
BbsI 1030 GTCTTC GAAGAC(2/6)
BpuAI 1030 GTCTTC GAAGAC(2/6)
BsaJI 1037 CCTTGG C^C[ACGT][ACGT]GG
BsgI 343 GTGCAG GTGCAG(16/14)
BsiEI 26 CGGCCG CG[GA][CT]^CG
Bsp1407I 410 TGTACA T^GTACA
BspMI 345 GCAGGT ACCTGC(4/8)
BsrFI 119 GCCGGT [GA]^CCGG[CT]
BsrGI 410 TGTACA T^GTACA
BstNI 643 CCAGG CC^[AT]GG
CfoI 327 GCGC GCG^C
Cfr10I 119 GCCGGT [GA]^CCGG[CT]
Cfr13I 512 GGGCC G^G[ACGT]CC
EaeI 26 CGGCCG [CT]^GGCC[GA]
EagI 26 CGGCCG C^GGCCG
EclXI 26 CGGCCG C^GGCCG
Eco47III 326 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
EcoRII 643 CCAGG ^CC[AT]GG
EcoT14I 1037 CCTTGG C^C[AT][AT]GG
HaeII 326 AGCGCT [GA]GCGC^[CT]
HhaI 327 GCGC GCG^C
HinP1I 327 GCGC G^CGC
HincII 205 GTCGAC GT[CT]^[GA]AC
HindII 205 GTCGAC GT[CT]^[GA]AC
HindIII 238 AAGCTT A^AGCTT
HphI 1034 TCACC GGTGA(8/7)
MaeII 1028 ACGT A^CGT
MaeIII 695 GTGAC ^GT[ACGT]AC
MfeI 473 CAATTG C^AATTG
MunI 473 CAATTG C^AATTG
MvaI 643 CCAGG CC^[AT]GG
NlaIII 483 CATG CATG^
NlaIV 348 GGTTCC GG[ACGT]^[ACGT]CC
PleI 647 GACTC GAGTC(4/5)
SalI 205 GTCGAC G^TCGAC
SapI 91 GAAGAGC GCTCTTC(1/4)
Sau96I 512 GGGCC G^G[ACGT]CC
ScaI 257 AGTACT AGT^ACT
SfcI 575 CTGTAG C^T[GA][CT]AG
SspBI 410 TGTACA T^GTACA
StyI 1037 CCTTGG C^C[AT][AT]GG
TfiI 893 GATTC G^A[AT]TC
Tsp45I 695 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 239 AGCT AG^CT
867 AGCT
BcnI 9 CCCGG CC^[GC]GG
29 CCGGG
BsiYI 446 CCAAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
515 CCTTGCGATGG
BslI 446 CCAAGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
515 CCTTGCGATGG
BstF5I 42 GGATG GGATG(2/0)
1041 GGATG
Cac8I 358 GCTCGC GC[ACGT]^[ACGT]GC
703 GCTTGC
FokI 42 GGATG GGATG(9/13)
1041 GGATG
HinfI 647 GACTC G^A[ACGT]TC
893 GATTC
MslI 822 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
837 CATTTTGATG
NciI 9 CCCGG CC^[GC]GG
29 CCGGG
SfaNI 769 GATGC GCATC(5/9)
1042 GATGC
TaqI 206 TCGA T^CGA
389 TCGA
TthHB8I 206 TCGA T^CGA
389 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AfaI AlwI ApoI Asp700I
BbvI BsrI BssKI BstYI DdeI
DpnII EarI Fnu4HI HaeIII HapII
HpaII ItaI Ksp632I MboI MboII
MflI MnlI MseI MspI MwoI
NdeII RsaI Sau3AI ScrFI Tru9I
TseI Tsp509I TspRI XhoII XmnI