Restriction map for
pFN21AB5654
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# NO CUT ENZYMES ( 158 enzymes) #
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Acc65I AccIII AciI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MslI MspI MunI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 143 GACGTC GACGT^C
AccI 796 GTCTAC GT^[AC][GT]AC
AccII 761 CGCG CG^CG
AcyI 143 GACGTC G[GA]^CG[CT]C
AflIII 667 ACATGT A^C[GA][CT]GT
ApaI 437 GGGCCC GGGCC^C
BalI 725 TGGCCA TGG^CCA
BanII 437 GGGCCC G[GA]GC[CT]^C
BfaI 968 CTAG C^TAG
BsaHI 143 GACGTC G[GA]^CG[CT]C
BseRI 33 CTCCTC GAGGAG(10/8)
BsmBI 145 CGTCTC CGTCTC(1/5)
BspHI 551 TCATGA T^CATGA
BspLU11I 667 ACATGT A^CATGT
BsrDI 539 GCAATG GCAATG(2/0)
BstUI 761 CGCG CG^CG
BstXI 903 CCACAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 60 AGATCC [GA]^GATC[CT]
CfoI 760 GCGC GCG^C
DsaI 623 CCATGG C^C[GA][CT]GG
EaeI 725 TGGCCA [CT]^GGCC[GA]
EcoT14I 623 CCATGG C^C[AT][AT]GG
HhaI 760 GCGC GCG^C
Hin1I 143 GACGTC G[GA]^CG[CT]C
HinP1I 760 GCGC G^CGC
MaeI 968 CTAG C^TAG
MaeII 144 ACGT A^CGT
MaeIII 379 GTTAC ^GT[ACGT]AC
MflI 60 AGATCC [GA]^GATC[CT]
MluNI 725 TGGCCA TGG^CCA
MscI 725 TGGCCA TGG^CCA
MseI 863 TTAA T^TAA
MvnI 761 CGCG CG^CG
NcoI 623 CCATGG C^CATGG
NheI 967 GCTAGC G^CTAGC
NspI 667 ACATGT [GA]CATG^[CT]
PstI 38 CTGCAG CTGCA^G
RcaI 551 TCATGA T^CATGA
SexAI 497 ACCTGGT A^CC[AT]GGT
StyI 623 CCATGG C^C[AT][AT]GG
TaqI 593 TCGA T^CGA
Tru9I 863 TTAA T^TAA
Tsp509I 334 AATT ^AATT
TthHB8I 593 TCGA T^CGA
XhoII 60 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwNI 493 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
740 CAGTGTCTG
AspHI 19 GTGCTC G[AT]GC[AT]^C
30 GTGCTC
BbvI 536 GCTGC GCAGC(8/12)
661 GCTGC
BpmI 190 CTGGAG CTGGAG(16/14)
360 CTGGAG
BsiHKAI 19 GTGCTC G[AT]GC[AT]^C
30 GTGCTC
BsiYI 293 CCAGCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
492 CCAGAACCTGG
BslI 293 CCAGCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
492 CCAGAACCTGG
BsmI 206 GAATGC GAATGC(1/-1)
195 GCATTC
Cac8I 412 GCTTGC GC[ACGT]^[ACGT]GC
967 GCTAGC
DraII 436 GGGGCCC [GA]G^G[ACGT]CC[CT]
437 GGGCCCT
EcoO109I 436 GGGGCCC [GA]G^G[ACGT]CC[CT]
437 GGGCCCT
Fnu4HI 536 GCTGC GC^[ACGT]GC
661 GCTGC
HgaI 601 GACGC GACGC(5/10)
762 GCGTC
ItaI 536 GCTGC GC^[ACGT]GC
661 GCTGC
MspA1I 451 CAGCTG C[AC]G^C[GT]G
894 CAGCTG
PflMI 293 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
492 CCAGAACCTGG
PleI 595 GACTC GAGTC(4/5)
646 GACTC
PvuII 451 CAGCTG CAG^CTG
894 CAGCTG
SfcI 38 CTGCAG C^T[GA][CT]AG
783 CTACAG
TseI 536 GCTGC G^C[AT]GC
661 GCTGC
Van91I 293 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
492 CCAGAACCTGG
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI AlwI BmyI BsaJI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
FokI HaeIII HinfI HphI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI TspRI