Restriction map for
pFN21AE9553
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BglII BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1897 GTCGAC GT^[AC][GT]AC
AflII 493 CTTAAG C^TTAAG
AocI 352 CCTGAGG CC^T[ACGT]AGG
BamHI 341 GGATCC G^GATCC
BanII 1144 GGGCTC G[GA]GC[CT]^C
BclI 1353 TGATCA T^GATCA
BcnI 2067 CCCGG CC^[GC]GG
BfrI 493 CTTAAG C^TTAAG
BglI 1910 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1875 CTCCAG CTGGAG(16/14)
BseRI 355 GAGGAG GAGGAG(10/8)
BspLU11I 560 ACATGT A^CATGT
BstAPI 770 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1244 TTCGAA TT^CGAA
BstXI 1664 CCAAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
DraII 2009 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 888 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 811 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1209 TGGCCG [CT]^GGCC[GA]
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2009 AGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 1353 TGATCA T^GATCA
KspI 161 CCGCGG CCGC^GG
NciI 2067 CCCGG CC^[GC]GG
NcoI 1670 CCATGG C^CATGG
NheI 1813 GCTAGC G^CTAGC
NspV 1244 TTCGAA TT^CGAA
PflMI 911 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2009 AGGTCCC [GA]G^G[AT]CC[CT]
SacII 161 CCGCGG CCGC^GG
SalI 1897 GTCGAC G^TCGAC
ScaI 1563 AGTACT AGT^ACT
SexAI 1002 ACCAGGT A^CC[AT]GGT
SfuI 1244 TTCGAA TT^CGAA
SmlI 493 CTTAAG C^T[CT][GA]AG
SspI 618 AATATT AAT^ATT
Van91I 911 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 459 CCAAAGAGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccII 162 CGCG CG^CG
1937 CGCG
AflIII 25 ACGTGT A^C[GA][CT]GT
560 ACATGT
AseI 324 ATTAAT AT^TAAT
1449 ATTAAT
AsnI 324 ATTAAT AT^TAAT
1449 ATTAAT
Asp700I 259 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
608 GAACAGTTTC
AvaII 433 GGTCC G^G[AT]CC
2010 GGTCC
AvrII 822 CCTAGG C^CTAGG
1740 CCTAGG
BbsI 1255 GTCTTC GAAGAC(2/6)
1471 GTCTTC
BlnI 822 CCTAGG C^CTAGG
1740 CCTAGG
BmyI 52 GGGCAC G[AGT]GC[ACT]^C
1144 GGGCTC
BpuAI 1255 GTCTTC GAAGAC(2/6)
1471 GTCTTC
BsaI 693 GAGACC GGTCTC(1/5)
1347 GGTCTC
BsiYI 911 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2068 CCGGTTTCAGG
BslI 911 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2068 CCGGTTTCAGG
BsmFI 507 GGGAC GGGAC(10/14)
2011 GTCCC
Bsp1286I 52 GGGCAC G[AGT]GC[ACT]^C
1144 GGGCTC
BstUI 162 CGCG CG^CG
1937 CGCG
BstYI 341 GGATCC [GA]^GATC[CT]
1539 AGATCC
CfoI 544 GCGC GCG^C
1936 GCGC
DraI 568 TTTAAA TTT^AAA
1519 TTTAAA
DsaI 161 CCGCGG C^C[GA][CT]GG
1670 CCATGG
HapII 1212 CCGG C^CGG
2068 CCGG
HhaI 544 GCGC GCG^C
1936 GCGC
HinP1I 544 GCGC G^CGC
1936 GCGC
HincII 21 GTTGAC GT[CT]^[GA]AC
1897 GTCGAC
HindII 21 GTTGAC GT[CT]^[GA]AC
1897 GTCGAC
HpaII 1212 CCGG C^CGG
2068 CCGG
MflI 341 GGATCC [GA]^GATC[CT]
1539 AGATCC
MspA1I 161 CCGCGG C[AC]G^C[GT]G
1260 CAGCGG
MspI 1212 CCGG C^CGG
2068 CCGG
MvnI 162 CGCG CG^CG
1937 CGCG
NspI 560 ACATGT [GA]CATG^[CT]
1495 ACATGC
PleI 1880 GACTC GAGTC(4/5)
1253 GAGTC
PshBI 324 ATTAAT AT^TAAT
1449 ATTAAT
PstI 929 CTGCAG CTGCA^G
1712 CTGCAG
XhoII 341 GGATCC [GA]^GATC[CT]
1539 AGATCC
XmnI 259 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
608 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BsaJI BsmAI
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I