Restriction map for
pFN21AE9540
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BpmI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
Eam1105I Eco47III Eco81I EcoRI EcoT14I
FbaI FseI HincII HindII HindIII
HpaI KpnI MaeII MamI MboI
MfeI MflI MluI MroI MseI
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI Sau3AI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 705 GTAC GT^AC
AflIII 236 ACATGT A^C[GA][CT]GT
AvaII 771 GGACC G^G[AT]CC
AviII 635 TGCGCA TGC^GCA
BalI 404 TGGCCA TGG^CCA
BanI 678 GGCGCC G^G[CT][GA]CC
BbeI 678 GGCGCC GGCGC^C
BbsI 1114 GTCTTC GAAGAC(2/6)
BcgI 759 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1166 CTAG C^TAG
BlpI 891 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 891 GCTCAGC GC^T[ACGT]AGC
BpuAI 1114 GTCTTC GAAGAC(2/6)
BspLU11I 236 ACATGT A^CATGT
BsrDI 408 CATTGC GCAATG(2/0)
BsrFI 212 ACCGGC [GA]^CCGG[CT]
BstEII 915 GGTCACC G^GT[ACGT]ACC
BstF5I 243 CATCC GGATG(2/0)
BstPI 915 GGTCACC G^GT[ACGT]ACC
CelII 891 GCTCAGC GC^T[ACGT]AGC
Cfr10I 212 ACCGGC [GA]^CCGG[CT]
DraIII 318 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 658 CTCTTC CTCTTC(1/4)
EcoNI 462 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 915 GGTCACC G^GT[ACGT]ACC
EcoRV 1285 GATATC GAT^ATC
EcoT22I 220 ATGCAT ATGCA^T
FokI 243 CATCC GGATG(9/13)
FspI 635 TGCGCA TGC^GCA
HgaI 574 GACGC GACGC(5/10)
KasI 678 GGCGCC G^GCGCC
Ksp632I 658 CTCTTC CTCTTC(1/4)
KspI 1011 CCGCGG CCGC^GG
MaeI 1166 CTAG C^TAG
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
NarI 678 GGCGCC GG^CGCC
NsiI 220 ATGCAT ATGCA^T
PflMI 561 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 904 CAGCTG CAG^CTG
RsaI 705 GTAC GT^AC
SacII 1011 CCGCGG CCGC^GG
SapI 657 GCTCTTC GCTCTTC(1/4)
SexAI 911 ACCTGGT A^CC[AT]GGT
SfoI 678 GGCGCC GGC^GCC
SmlI 205 CTCAAG C^T[CT][GA]AG
Van91I 561 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 643 GTCTAC GT^[AC][GT]AC
922 GTCTAC
AcyI 574 GACGCC G[GA]^CG[CT]C
678 GGCGCC
Alw44I 436 GTGCAC G^TGCAC
805 GTGCAC
ApaLI 436 GTGCAC G^TGCAC
805 GTGCAC
BglI 70 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1089 GCCCGGCGGGC
BsaHI 574 GACGCC G[GA]^CG[CT]C
678 GGCGCC
BsiEI 527 CGGCCG CG[GA][CT]^CG
1188 CGGCCG
BsmFI 55 GGGAC GGGAC(10/14)
521 GTCCC
BspMI 266 ACCTGC ACCTGC(4/8)
1259 ACCTGC
BstAPI 278 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
722 GCAAGCTCTGC
BstXI 296 CCACCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
407 CCATTGCTGTGG
DsaI 1011 CCGCGG C^C[GA][CT]GG
1224 CCACGG
EagI 527 CGGCCG C^GGCCG
1188 CGGCCG
EclXI 527 CGGCCG C^GGCCG
1188 CGGCCG
Eco52I 527 CGGCCG C^GGCCG
1188 CGGCCG
Hin1I 574 GACGCC G[GA]^CG[CT]C
678 GGCGCC
HinfI 1138 GACTC G^A[ACGT]TC
1156 GAGTC
PleI 1138 GACTC GAGTC(4/5)
1156 GAGTC
SmaI 738 CCCGGG CCC^GGG
751 CCCGGG
SphI 287 GCATGC GCATG^C
632 GCATGC
StuI 614 AGGCCT AGG^CCT
1073 AGGCCT
TaqI 332 TCGA T^CGA
878 TCGA
TthHB8I 332 TCGA T^CGA
878 TCGA
XcmI 131 CCATGTTCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
293 CCACCACCCTTGTGG
XmaCI 738 CCCGGG C^CCGGG
751 CCCGGG
XmaI 738 CCCGGG C^CCGGG
751 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI BanII BbvI BcnI BmyI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrBI BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI