Restriction map for
pFN21AB8746
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MroI MseI
MslI MunI NarI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 420 GGCGTC G[GA]^CG[CT]C
AfaI 71 GTAC GT^AC
BalI 173 TGGCCA TGG^CCA
BanII 14 GAGCCC G[GA]GC[CT]^C
BpmI 298 CTGGAG CTGGAG(16/14)
BsaHI 420 GGCGTC G[GA]^CG[CT]C
BsmFI 449 GGGAC GGGAC(10/14)
EarI 488 CTCTTC CTCTTC(1/4)
EcoNI 321 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 456 GGCGCT [GA]GCGC^[CT]
Hin1I 420 GGCGTC G[GA]^CG[CT]C
HinfI 125 GACTC G^A[ACGT]TC
HphI 278 TCACC GGTGA(8/7)
Ksp632I 488 CTCTTC CTCTTC(1/4)
MaeII 452 ACGT A^CGT
MboII 489 TCTTC GAAGA(8/7)
MluNI 173 TGGCCA TGG^CCA
MscI 173 TGGCCA TGG^CCA
PleI 125 GACTC GAGTC(4/5)
RsaI 71 GTAC GT^AC
SapI 487 GCTCTTC GCTCTTC(1/4)
SfiI 364 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 239 TCGA T^CGA
TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 239 TCGA T^CGA
XcmI 515 CCACAGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 79 CGCG CG^CG
474 CGCG
AvaII 303 GGACC G^G[AT]CC
330 GGTCC
BglI 142 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
365 GCCCGCTGGGC
BmyI 14 GAGCCC G[AGT]GC[ACT]^C
196 GTGCCC
Bsp1286I 14 GAGCCC G[AGT]GC[ACT]^C
196 GTGCCC
BsrFI 147 GCCGGC [GA]^CCGG[CT]
374 GCCGGC
BstUI 79 CGCG CG^CG
474 CGCG
Cfr10I 147 GCCGGC [GA]^CCGG[CT]
374 GCCGGC
DraII 317 AGGCCCT [GA]G^G[ACGT]CC[CT]
521 AGGCCCT
EaeI 173 TGGCCA [CT]^GGCC[GA]
470 TGGCCG
EcoO109I 317 AGGCCCT [GA]G^G[ACGT]CC[CT]
521 AGGCCCT
HgaI 246 GACGC GACGC(5/10)
421 GCGTC
MaeIII 244 GTGAC ^GT[ACGT]AC
266 GTGAC
MvnI 79 CGCG CG^CG
474 CGCG
NaeI 147 GCCGGC GCC^GGC
374 GCCGGC
NcoI 92 CCATGG C^CATGG
360 CCATGG
NgoMIV 147 GCCGGC G^CCGGC
374 GCCGGC
SmaI 201 CCCGGG CCC^GGG
435 CCCGGG
Tsp45I 244 GTGAC ^GT[GC]AC
266 GTGAC
XmaCI 201 CCCGGG C^CCGGG
435 CCCGGG
XmaI 201 CCCGGG C^CCGGG
435 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsiYI BslI BsoBI BspMI
BssKI BstNI Cac8I CfoI Cfr13I
DsaI EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PstI
PvuII Sau96I ScrFI SfcI StyI
TseI