Restriction map for
pFN21AB8722
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AclI AcyI AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BbrPI BcgI BciVI BfaI
BlnI BlpI Bpu1102I BsaBI BsaHI
BsiWI BsmBI BsmI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MaeI MamI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccIII 974 TCCGGA T^CCGGA
AcsI 96 AAATTT [GA]^AATT[CT]
AflII 208 CTTAAG C^TTAAG
AhdI 250 GACGGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1551 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1264 CCTAAGG CC^T[ACGT]AGG
Aor51HI 739 AGCGCT AGC^GCT
ApoI 96 AAATTT [GA]^AATT[CT]
Asp700I 1041 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 250 GACGGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 628 GAGCTC G[AT]GC[AT]^C
AvaI 611 CCCGAG C^[CT]CG[GA]G
AviII 1335 TGCGCA TGC^GCA
BalI 1103 TGGCCA TGG^CCA
BanI 952 GGCACC G^G[CT][GA]CC
BbsI 1347 GAAGAC GAAGAC(2/6)
BbvCI 1095 CCTCAGC CCTCAGC(-5/-2)
BclI 263 TGATCA T^GATCA
BfrI 208 CTTAAG C^TTAAG
BglI 379 GCCTATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 81 AGATCT A^GATCT
BpuAI 1347 GAAGAC GAAGAC(2/6)
BsaAI 841 TACGTG [CT]AC^GT[GA]
BsaWI 974 TCCGGA [AT]^CCGG[AT]
BseAI 974 TCCGGA T^CCGGA
BsiEI 362 CGACCG CG[GA][CT]^CG
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
BsoBI 611 CCCGAG C^[CT]CG[GA]G
Bsp1407I 32 TGTACA T^GTACA
BspEI 974 TCCGGA T^CCGGA
BsrBI 1342 GAGCGG CCGCTC(-3/-3)
BsrDI 233 GCAATG GCAATG(2/0)
BsrFI 364 ACCGGC [GA]^CCGG[CT]
BsrGI 32 TGTACA T^GTACA
Bsu36I 1264 CCTAAGG CC^T[ACGT]AGG
Cfr10I 364 ACCGGC [GA]^CCGG[CT]
DraIII 343 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1103 TGGCCA [CT]^GGCC[GA]
Eam1105I 250 GACGGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 739 AGCGCT AGC^GCT
Eco81I 1264 CCTAAGG CC^T[ACGT]AGG
FbaI 263 TGATCA T^GATCA
FspI 1335 TGCGCA TGC^GCA
HaeII 739 AGCGCT [GA]GCGC^[CT]
KspI 1086 CCGCGG CCGC^GG
MaeIII 1244 GTGAC ^GT[ACGT]AC
MluNI 1103 TGGCCA TGG^CCA
MroI 974 TCCGGA T^CCGGA
MscI 1103 TGGCCA TGG^CCA
MseI 209 TTAA T^TAA
PleI 1027 GAGTC GAGTC(4/5)
PvuII 788 CAGCTG CAG^CTG
SacI 628 GAGCTC GAGCT^C
SacII 1086 CCGCGG CCGC^GG
SbfI 621 CCTGCAGG CCTGCA^GG
Sse8387I 621 CCTGCAGG CCTGCA^GG
SspBI 32 TGTACA T^GTACA
TaqI 797 TCGA T^CGA
TfiI 657 GATTC G^A[AT]TC
Tru9I 209 TTAA T^TAA
Tsp45I 1244 GTGAC ^GT[GC]AC
TthHB8I 797 TCGA T^CGA
XcmI 1226 CCATGGAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1041 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 148 GTCTAC GT^[AC][GT]AC
373 GTCTAC
AccII 759 CGCG CG^CG
1087 CGCG
ApaI 1114 GGGCCC GGGCC^C
1413 GGGCCC
BpmI 472 CTCCAG CTGGAG(16/14)
877 CTGGAG
BseRI 1270 GAGGAG GAGGAG(10/8)
1315 GAGGAG
BspMI 428 ACCTGC ACCTGC(4/8)
907 ACCTGC
BstUI 759 CGCG CG^CG
1087 CGCG
BstXI 482 CCACCAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
755 CCACCGCGATGG
Cac8I 1391 GCCAGC GC[ACGT]^[ACGT]GC
1470 GCGAGC
DdeI 1096 CTCAG C^T[ACGT]AG
1265 CTAAG
HinfI 657 GATTC G^A[ACGT]TC
1027 GAGTC
MspA1I 788 CAGCTG C[AC]G^C[GT]G
1086 CCGCGG
MvnI 759 CGCG CG^CG
1087 CGCG
NcoI 202 CCATGG C^CATGG
1226 CCATGG
PflMI 342 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGGGCGTGG
SapI 1383 GAAGAGC GCTCTTC(1/4)
1483 GAAGAGC
SmlI 208 CTTAAG C^T[CT][GA]AG
1357 CTCAAG
Van91I 342 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1431 CCAGGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI CfoI Cfr13I DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp509I
TspRI XhoII