Restriction map for
pFN21AB8621
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbvCI BcgI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 708 GGTACC G^GTACC
AflIII 1971 ACATGT A^C[GA][CT]GT
AlwI 760 GGATC GGATC(4/5)
AlwNI 1957 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1031 CCTCAGG CC^T[ACGT]AGG
AseI 2533 ATTAAT AT^TAAT
AsnI 2533 ATTAAT AT^TAAT
Asp700I 724 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 708 GGTACC G^GTACC
BclI 1562 TGATCA T^GATCA
BcnI 2472 CCGGG CC^[GC]GG
BseRI 1711 GAGGAG GAGGAG(10/8)
BsgI 879 GTGCAG GTGCAG(16/14)
BsiEI 2391 CGACCG CG[GA][CT]^CG
BsmFI 621 GGGAC GGGAC(10/14)
Bsp1407I 1349 TGTACA T^GTACA
BspLU11I 1971 ACATGT A^CATGT
BspMI 15 ACCTGC ACCTGC(4/8)
BsrGI 1349 TGTACA T^GTACA
BstXI 2046 CCAAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1031 CCTCAGG CC^T[ACGT]AGG
DraIII 1678 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1031 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1787 CCATGG C^C[AT][AT]GG
EcoT22I 947 ATGCAT ATGCA^T
FbaI 1562 TGATCA T^GATCA
HapII 2472 CCGG C^CGG
HincII 2252 GTCAAC GT[CT]^[GA]AC
HindII 2252 GTCAAC GT[CT]^[GA]AC
HpaII 2472 CCGG C^CGG
KpnI 708 GGTACC GGTAC^C
MaeII 1076 ACGT A^CGT
MspI 2472 CCGG C^CGG
NciI 2472 CCGGG CC^[GC]GG
NcoI 1787 CCATGG C^CATGG
NsiI 947 ATGCAT ATGCA^T
NspI 1971 ACATGT [GA]CATG^[CT]
PflMI 1677 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2533 ATTAAT AT^TAAT
SexAI 2354 ACCTGGT A^CC[AT]GGT
SspBI 1349 TGTACA T^GTACA
StuI 1383 AGGCCT AGG^CCT
StyI 1787 CCATGG C^C[AT][AT]GG
Van91I 1677 CCACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 724 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AvaII 299 GGTCC G^G[AT]CC
1110 GGACC
BanI 516 GGCACC G^G[CT][GA]CC
708 GGTACC
BciVI 448 GGATAC GTATCC(6/5)
2285 GTATCC
BfaI 217 CTAG C^TAG
1390 CTAG
BpmI 231 CTCCAG CTGGAG(16/14)
496 CTCCAG
BsmI 142 GAATGC GAATGC(1/-1)
2577 GCATTC
BsrDI 33 CATTGC GCAATG(2/0)
1257 CATTGC
Cac8I 1603 GCTGGC GC[ACGT]^[ACGT]GC
2363 GCTGGC
DsaI 1787 CCATGG C^C[GA][CT]GG
2394 CCGTGG
HindIII 329 AAGCTT A^AGCTT
464 AAGCTT
MaeI 217 CTAG C^TAG
1390 CTAG
NdeI 1127 CATATG CA^TATG
1234 CATATG
PstI 17 CTGCAG CTGCA^G
1587 CTGCAG
ScaI 831 AGTACT AGT^ACT
2480 AGTACT
SfaNI 677 GCATC GCATC(5/9)
1592 GCATC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbsI
BbvI BpuAI BsaI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I