Restriction map for
pFN21AB8617
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaII AviII
AvrII BbeI BbrPI BbvCI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 499 GTCTAC GT^[AC][GT]AC
AciI 1438 CCGC CCGC(-3/-1)
AfaI 1249 GTAC GT^AC
AflIII 963 ACATGT A^C[GA][CT]GT
AocI 631 CCTGAGG CC^T[ACGT]AGG
Aor51HI 293 AGCGCT AGC^GCT
AvaI 229 CCCGAG C^[CT]CG[GA]G
BamHI 1702 GGATCC G^GATCC
BanI 672 GGTGCC G^G[CT][GA]CC
BcgI 231 CGAGCAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1532 TGATCA T^GATCA
BglI 191 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 737 GTGCAG GTGCAG(16/14)
BsoBI 229 CCCGAG C^[CT]CG[GA]G
BspHI 1743 TCATGA T^CATGA
BspLU11I 963 ACATGT A^CATGT
BstXI 1590 CCATCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
Cfr13I 599 GGCCC G^G[ACGT]CC
DraI 1308 TTTAAA TTT^AAA
Eco47III 293 AGCGCT AGC^GCT
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
FbaI 1532 TGATCA T^GATCA
HaeII 293 AGCGCT [GA]GCGC^[CT]
HphI 593 TCACC GGTGA(8/7)
MaeII 1251 ACGT A^CGT
MslI 684 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 1179 GCTAGC G^CTAGC
NspI 963 ACATGT [GA]CATG^[CT]
RcaI 1743 TCATGA T^CATGA
RsaI 1249 GTAC GT^AC
SacI 1017 GAGCTC GAGCT^C
SapI 262 GAAGAGC GCTCTTC(1/4)
Sau96I 599 GGCCC G^G[ACGT]CC
SfaNI 127 GATGC GCATC(5/9)
StuI 1521 AGGCCT AGG^CCT
TaqI 800 TCGA T^CGA
Tsp45I 592 GTCAC ^GT[GC]AC
TthHB8I 800 TCGA T^CGA
XcmI 1070 CCATGTTAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 1703 GATCC GGATC(4/5)
1702 GGATC
BalI 198 TGGCCA TGG^CCA
731 TGGCCA
BanII 265 GAGCCC G[GA]GC[CT]^C
1017 GAGCTC
BglII 13 AGATCT A^GATCT
1371 AGATCT
BpmI 1334 CTCCAG CTGGAG(16/14)
1220 CTGGAG
BsmI 33 GAATGC GAATGC(1/-1)
486 GAATGC
BstF5I 253 CATCC GGATG(2/0)
1578 GGATG
CfoI 294 GCGC GCG^C
820 GCGC
EaeI 198 TGGCCA [CT]^GGCC[GA]
731 TGGCCA
FokI 253 CATCC GGATG(9/13)
1578 GGATG
HhaI 294 GCGC GCG^C
820 GCGC
HinP1I 294 GCGC G^CGC
820 GCGC
MaeIII 592 GTCAC ^GT[ACGT]AC
1798 GTTAC
MluNI 198 TGGCCA TGG^CCA
731 TGGCCA
MscI 198 TGGCCA TGG^CCA
731 TGGCCA
MspA1I 562 CAGCTG C[AC]G^C[GT]G
761 CAGCTG
PvuII 562 CAGCTG CAG^CTG
761 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI AlwNI ApoI Asp700I
AspHI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeI MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfcI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI