Restriction map for
pFN21AB5174
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BsaBI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MaeIII MamI
MfeI MluI MroI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AvaII 701 GGACC G^G[AT]CC
AviII 749 TGCGCA TGC^GCA
BbsI 343 GTCTTC GAAGAC(2/6)
BbvCI 1024 GCTGAGG CCTCAGC(-5/-2)
BpuAI 343 GTCTTC GAAGAC(2/6)
BsaAI 577 TACGTG [CT]AC^GT[GA]
BsaI 989 GAGACC GGTCTC(1/5)
BseRI 490 CTCCTC GAGGAG(10/8)
BsiEI 19 CGGCCG CG[GA][CT]^CG
BsmBI 432 CGTCTC CGTCTC(1/5)
BspMI 935 ACCTGC ACCTGC(4/8)
BstXI 383 CCATCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 243 GGATCT [GA]^GATC[CT]
EagI 19 CGGCCG C^GGCCG
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
FspI 749 TGCGCA TGC^GCA
MflI 243 GGATCT [GA]^GATC[CT]
NspI 784 GCATGT [GA]CATG^[CT]
SapI 582 GCTCTTC GCTCTTC(1/4)
SbfI 855 CCTGCAGG CCTGCA^GG
SgrAI 26 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 855 CCTGCAGG CCTGCA^GG
StuI 572 AGGCCT AGG^CCT
TfiI 159 GAATC G^A[AT]TC
Tsp509I 520 AATT ^AATT
XcmI 505 CCAGCGCTGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 243 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 655 GTAC GT^AC
850 GTAC
AluI 71 AGCT AG^CT
1075 AGCT
Alw44I 626 GTGCAC G^TGCAC
1148 GTGCAC
AlwI 865 GATCC GGATC(4/5)
243 GGATC
Aor51HI 507 AGCGCT AGC^GCT
1091 AGCGCT
ApaI 421 GGGCCC GGGCC^C
792 GGGCCC
ApaLI 626 GTGCAC G^TGCAC
1148 GTGCAC
BfaI 154 CTAG C^TAG
163 CTAG
BglI 678 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
775 GCCTTTGTGGC
BlpI 369 GCTGAGC GC^T[ACGT]AGC
756 GCTCAGC
BpmI 435 CTCCAG CTGGAG(16/14)
391 CTGGAG
Bpu1102I 369 GCTGAGC GC^T[ACGT]AGC
756 GCTCAGC
BsmAI 989 GAGAC GTCTC(1/5)
433 GTCTC
BsrBI 292 CCGCTC CCGCTC(-3/-3)
559 CCGCTC
BsrI 879 ACTGG ACTGG(1/-1)
437 CCAGT
BstAPI 352 GCACTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
641 GCATCTACTGC
BstF5I 276 CATCC GGATG(2/0)
609 CATCC
CelII 369 GCTGAGC GC^T[ACGT]AGC
756 GCTCAGC
EarI 583 CTCTTC CTCTTC(1/4)
799 CTCTTC
Eco47III 507 AGCGCT AGC^GCT
1091 AGCGCT
FokI 276 CATCC GGATG(9/13)
609 CATCC
Ksp632I 583 CTCTTC CTCTTC(1/4)
799 CTCTTC
KspI 75 CCGCGG CCGC^GG
467 CCGCGG
MaeI 154 CTAG C^TAG
163 CTAG
NcoI 407 CCATGG C^CATGG
884 CCATGG
PleI 1167 GACTC GAGTC(4/5)
4 GAGTC
PstI 856 CTGCAG CTGCA^G
1187 CTGCAG
RsaI 655 GTAC GT^AC
850 GTAC
SacII 75 CCGCGG CCGC^GG
467 CCGCGG
SfaNI 608 GCATC GCATC(5/9)
641 GCATC
SfcI 856 CTGCAG C^T[GA][CT]AG
1187 CTGCAG
TaqI 709 TCGA T^CGA
818 TCGA
TspRI 357 CACTG CA[GC]TG[ACGT][ACGT]^
549 CACTG
TthHB8I 709 TCGA T^CGA
818 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AspHI AvaI
BalI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV Sau3AI
Sau96I ScrFI SfoI SmaI StyI
TseI XmaCI XmaI