################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI AscI AspEI AvrII BalI BbrPI BbvCI BcgI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BspEI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DrdI Eam1105I Eco47III EcoO65I FseI HpaI MamI MfeI MluI MluNI MroI MscI MslI MunI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 2749 GGTACC G^GTACC AcsI 1882 GAATTC [GA]^AATT[CT] AflIII 1051 ACATGT A^C[GA][CT]GT Alw44I 1055 GTGCAC G^TGCAC AocI 748 CCTGAGG CC^T[ACGT]AGG ApaLI 1055 GTGCAC G^TGCAC ApoI 1882 GAATTC [GA]^AATT[CT] AseI 2584 ATTAAT AT^TAAT AsnI 2584 ATTAAT AT^TAAT Asp700I 3393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2749 GGTACC G^GTACC AviII 1130 TGCGCA TGC^GCA BbeI 829 GGCGCC GGCGC^C BciVI 2389 GTATCC GTATCC(6/5) BclI 3278 TGATCA T^GATCA BglII 1457 AGATCT A^GATCT BlpI 1597 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1597 GCTGAGC GC^T[ACGT]AGC BsiEI 2744 CGGCCG CG[GA][CT]^CG Bsp1407I 714 TGTACA T^GTACA BspHI 1076 TCATGA T^CATGA BspLU11I 1051 ACATGT A^CATGT BsrBI 1678 GAGCGG CCGCTC(-3/-3) BsrDI 677 GCAATG GCAATG(2/0) BsrGI 714 TGTACA T^GTACA Bsu36I 748 CCTGAGG CC^T[ACGT]AGG CelII 1597 GCTGAGC GC^T[ACGT]AGC EagI 2744 CGGCCG C^GGCCG EclXI 2744 CGGCCG C^GGCCG Eco52I 2744 CGGCCG C^GGCCG Eco81I 748 CCTGAGG CC^T[ACGT]AGG EcoRI 1882 GAATTC G^AATTC EcoRV 208 GATATC GAT^ATC EcoT22I 317 ATGCAT ATGCA^T FbaI 3278 TGATCA T^GATCA FspI 1130 TGCGCA TGC^GCA HincII 1852 GTCAAC GT[CT]^[GA]AC HindII 1852 GTCAAC GT[CT]^[GA]AC HindIII 3074 AAGCTT A^AGCTT KasI 829 GGCGCC G^GCGCC KpnI 2749 GGTACC GGTAC^C KspI 3210 CCGCGG CCGC^GG NaeI 41 GCCGGC GCC^GGC NarI 829 GGCGCC GG^CGCC NdeI 98 CATATG CA^TATG NgoMIV 41 GCCGGC G^CCGGC NsiI 317 ATGCAT ATGCA^T PaeR7I 3128 CTCGAG C^TCGAG PshBI 2584 ATTAAT AT^TAAT RcaI 1076 TCATGA T^CATGA SacI 2641 GAGCTC GAGCT^C SacII 3210 CCGCGG CCGC^GG SfoI 829 GGCGCC GGC^GCC SphI 315 GCATGC GCATG^C SspBI 714 TGTACA T^GTACA StuI 2444 AGGCCT AGG^CCT XhoI 3128 CTCGAG C^TCGAG XmnI 3393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 127 GTAGAC GT^[AC][GT]AC 562 GTAGAC AspI 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3451 GACAAAGTC BamHI 884 GGATCC G^GATCC 1824 GGATCC BspDI 625 ATCGAT AT^CGAT 3283 ATCGAT ClaI 625 ATCGAT AT^CGAT 3283 ATCGAT DraIII 1163 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2756 CACTTAGTG MaeII 2177 ACGT A^CGT 3350 ACGT NcoI 1757 CCATGG C^CATGG 2859 CCATGG NspI 315 GCATGC [GA]CATG^[CT] 1051 ACATGT PflFI 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3451 GACAAAGTC PflMI 2755 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2859 CCATGGTGTGG SexAI 1164 ACCAGGT A^CC[AT]GGT 2258 ACCTGGT SmaI 83 CCCGGG CCC^GGG 3299 CCCGGG Tth111I 2833 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 3451 GACAAAGTC Van91I 2755 CCACTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2859 CCATGGTGTGG XcmI 350 CCAGCTTCCGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 720 CCAGGAGTGCTATGG XmaCI 83 CCCGGG C^CCGGG 3299 CCCGGG XmaI 83 CCCGGG C^CCGGG 3299 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII