Restriction map for
pFN21AA1207
##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI AscI
Asp718I AspEI AviII AvrII BcgI
BlnI BsaBI BsiWI BspDI BspLU11I
BssHII BstAPI ClaI CpoI Eam1105I
Eco47III EcoRV FseI FspI HpaI
KpnI KspI MamI MluI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PvuI RsrII SacII SalI
SbfI SexAI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2642 GACGTC GACGT^C
AccIII 5772 TCCGGA T^CCGGA
AclI 1491 AACGTT AA^CGTT
AflIII 2111 ACACGT A^C[GA][CT]GT
Alw44I 4173 GTGCAC G^TGCAC
ApaLI 4173 GTGCAC G^TGCAC
BbvCI 1099 GCTGAGG CCTCAGC(-5/-2)
BglII 5506 AGATCT A^GATCT
BlpI 526 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 526 GCTCAGC GC^T[ACGT]AGC
BseAI 5772 TCCGGA T^CCGGA
BsiEI 2843 CGGCCG CG[GA][CT]^CG
BsmBI 996 GAGACG CGTCTC(1/5)
BspEI 5772 TCCGGA T^CCGGA
Bst1107I 167 GTATAC GTA^TAC
BstBI 5630 TTCGAA TT^CGAA
BstZ17I 167 GTATAC GTA^TAC
CelII 526 GCTCAGC GC^T[ACGT]AGC
DraI 3274 TTTAAA TTT^AAA
DraIII 3254 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3122 CCATGG C^C[GA][CT]GG
EagI 2843 CGGCCG C^GGCCG
EclXI 2843 CGGCCG C^GGCCG
Eco52I 2843 CGGCCG C^GGCCG
EcoRI 2369 GAATTC G^AATTC
MfeI 221 CAATTG C^AATTG
MroI 5772 TCCGGA T^CCGGA
MunI 221 CAATTG C^AATTG
NcoI 3122 CCATGG C^CATGG
NdeI 3485 CATATG CA^TATG
NspV 5630 TTCGAA TT^CGAA
PshAI 5713 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1491 AACGTT AA^CGTT
PstI 3797 CTGCAG CTGCA^G
SacI 4690 GAGCTC GAGCT^C
ScaI 4973 AGTACT AGT^ACT
SfiI 494 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5630 TTCGAA TT^CGAA
SphI 2312 GCATGC GCATG^C
SspI 1149 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 167 GTATAC GT^[AC][GT]AC
3406 GTCTAC
AccII 604 CGCG CG^CG
5878 CGCG
AlwNI 3219 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
5203 CAGGGGCTG
AocI 4379 CCTCAGG CC^T[ACGT]AGG
5193 CCTCAGG
AseI 237 ATTAAT AT^TAAT
2280 ATTAAT
AsnI 237 ATTAAT AT^TAAT
2280 ATTAAT
BamHI 60 GGATCC G^GATCC
1185 GGATCC
BbrPI 3552 CACGTG CAC^GTG
5221 CACGTG
BciVI 2030 GGATAC GTATCC(6/5)
2457 GTATCC
BglI 495 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3289 GCCCTTGGGGC
BsaAI 3552 CACGTG [CT]AC^GT[GA]
5221 CACGTG
BsaI 5013 GAGACC GGTCTC(1/5)
2183 GGTCTC
BsgI 5146 CTGCAC GTGCAG(16/14)
3260 GTGCAG
BsmI 2409 GAATGC GAATGC(1/-1)
4654 GAATGC
BspHI 884 TCATGA T^CATGA
4073 TCATGA
BsrFI 563 GCCGGC [GA]^CCGG[CT]
2841 GCCGGC
BssSI 2103 CACGAG CACGAG(-5/-1)
1180 CTCGTG
BstEII 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
BstPI 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
BstUI 604 CGCG CG^CG
5878 CGCG
Bsu36I 4379 CCTCAGG CC^T[ACGT]AGG
5193 CCTCAGG
Cfr10I 563 GCCGGC [GA]^CCGG[CT]
2841 GCCGGC
DrdI 472 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5440 GACATCTACGTC
Eco81I 4379 CCTCAGG CC^T[ACGT]AGG
5193 CCTCAGG
EcoNI 2701 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4210 CCTTATGCAGG
EcoO65I 277 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
EcoT22I 2411 ATGCAT ATGCA^T
2933 ATGCAT
HincII 745 GTCAAC GT[CT]^[GA]AC
2898 GTTGAC
HindII 745 GTCAAC GT[CT]^[GA]AC
2898 GTTGAC
HindIII 1657 AAGCTT A^AGCTT
2751 AAGCTT
MvnI 604 CGCG CG^CG
5878 CGCG
NaeI 563 GCCGGC GCC^GGC
2841 GCCGGC
NgoMIV 563 GCCGGC G^CCGGC
2841 GCCGGC
NsiI 2411 ATGCAT ATGCA^T
2933 ATGCAT
PmaCI 3552 CACGTG CAC^GTG
5221 CACGTG
PmlI 3552 CACGTG CAC^GTG
5221 CACGTG
PpuMI 266 AGGACCT [GA]G^G[AT]CC[CT]
4948 GGGTCCC
PshBI 237 ATTAAT AT^TAAT
2280 ATTAAT
RcaI 884 TCATGA T^CATGA
4073 TCATGA
SmaI 2824 CCCGGG CCC^GGG
4886 CCCGGG
StuI 649 AGGCCT AGG^CCT
3892 AGGCCT
XmaCI 2824 CCCGGG C^CCGGG
4886 CCCGGG
XmaI 2824 CCCGGG C^CCGGG
4886 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI ApaI ApoI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BclI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI