Restriction map for
pFN21AB5485
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BamHI BbrPI BbvCI BciVI
BfaI BfrI BlnI BseAI BsiEI
BsiHKAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HinfI
HpaI KpnI KspI MaeI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 555 GAATTT [GA]^AATT[CT]
AcyI 278 GGCGCC G[GA]^CG[CT]C
AlwNI 1267 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 555 GAATTT [GA]^AATT[CT]
Asp700I 153 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 418 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 662 TGGCCA TGG^CCA
BanI 278 GGCGCC G^G[CT][GA]CC
BbeI 278 GGCGCC GGCGC^C
BcgI 1226 GCATGACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 671 TGATCA T^GATCA
BglII 1247 AGATCT A^GATCT
BlpI 631 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 631 GCTGAGC GC^T[ACGT]AGC
BsaAI 1320 CACGTA [CT]AC^GT[GA]
BsaBI 1263 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 278 GGCGCC G[GA]^CG[CT]C
BsaI 367 GAGACC GGTCTC(1/5)
BsiWI 1322 CGTACG C^GTACG
BsmI 775 GCATTC GAATGC(1/-1)
BspMI 1080 GCAGGT ACCTGC(4/8)
BssHII 6 GCGCGC G^CGCGC
CelII 631 GCTGAGC GC^T[ACGT]AGC
DraIII 1398 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 864 GAAGAG CTCTTC(1/4)
FbaI 671 TGATCA T^GATCA
HaeII 278 GGCGCC [GA]GCGC^[CT]
HgaI 289 GACGC GACGC(5/10)
Hin1I 278 GGCGCC G[GA]^CG[CT]C
KasI 278 GGCGCC G^GCGCC
Ksp632I 864 GAAGAG CTCTTC(1/4)
MamI 1263 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
MseI 1100 TTAA T^TAA
NarI 278 GGCGCC GG^CGCC
PflFI 418 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1495 CTGCAG CTGCA^G
SapI 864 GAAGAGC GCTCTTC(1/4)
SexAI 404 ACCTGGT A^CC[AT]GGT
SfcI 1495 CTGCAG C^T[GA][CT]AG
SfiI 243 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 278 GGCGCC GGC^GCC
SmlI 835 CTCAAG C^T[CT][GA]AG
TaqI 335 TCGA T^CGA
Tru9I 1100 TTAA T^TAA
Tth111I 418 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 335 TCGA T^CGA
XmnI 153 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 7 CGCG CG^CG
802 CGCG
AlwI 1107 GATCC GGATC(4/5)
1269 GATCC
ApaI 25 GGGCCC GGGCC^C
588 GGGCCC
BbsI 100 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BpmI 1387 CTCCAG CTGGAG(16/14)
182 CTGGAG
BpuAI 100 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BsaWI 344 ACCGGA [AT]^CCGG[AT]
437 TCCGGT
BseRI 1243 GAGGAG GAGGAG(10/8)
1291 GAGGAG
BsgI 979 GTGCAG GTGCAG(16/14)
1216 GTGCAG
BsrDI 744 CATTGC GCAATG(2/0)
1167 CATTGC
BstUI 7 CGCG CG^CG
802 CGCG
DraII 25 GGGCCCC [GA]G^G[ACGT]CC[CT]
1382 AGGCCCT
EcoNI 669 CCTGATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1374 CCTGTCCCAGG
EcoO109I 25 GGGCCCC [GA]G^G[ACGT]CC[CT]
1382 AGGCCCT
HincII 703 GTCAAC GT[CT]^[GA]AC
1408 GTCAAC
HindII 703 GTCAAC GT[CT]^[GA]AC
1408 GTCAAC
MaeII 611 ACGT A^CGT
1321 ACGT
MspA1I 262 CAGCTG C[AC]G^C[GT]G
1337 CAGCTG
MvnI 7 CGCG CG^CG
802 CGCG
PvuII 262 CAGCTG CAG^CTG
1337 CAGCTG
SmaI 248 CCCGGG CCC^GGG
1022 CCCGGG
XmaCI 248 CCCGGG C^CCGGG
1022 CCCGGG
XmaI 248 CCCGGG C^CCGGG
1022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TseI Tsp45I Tsp509I TspRI XcmI
XhoII