Restriction map for
pFN21AA1352
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspLU11I
BsrBI BssHII BssSI BstAPI BstBI
BstEII BstPI CelII CfoI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 224 GGTACC G^GTACC
AccII 2011 CGCG CG^CG
AflIII 188 ACACGT A^C[GA][CT]GT
AseI 1053 ATTAAT AT^TAAT
AsnI 1053 ATTAAT AT^TAAT
Asp700I 1313 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 224 GGTACC G^GTACC
AspHI 2602 GAGCAC G[AT]GC[AT]^C
AspI 1261 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3234 TGGCCA TGG^CCA
BcnI 1368 CCGGG CC^[GC]GG
BmyI 2602 GAGCAC G[AGT]GC[ACT]^C
BsaAI 2864 TACGTA [CT]AC^GT[GA]
BsaBI 625 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2546 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2602 GAGCAC G[AT]GC[AT]^C
Bsp1286I 2602 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 2300 TGTACA T^GTACA
BsrFI 1281 GCCGGT [GA]^CCGG[CT]
BsrGI 2300 TGTACA T^GTACA
Bst1107I 708 GTATAC GTA^TAC
BstUI 2011 CGCG CG^CG
BstZ17I 708 GTATAC GTA^TAC
Cfr10I 1281 GCCGGT [GA]^CCGG[CT]
DrdI 2902 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2273 CCATGG C^C[GA][CT]GG
EcoRI 1987 GAATTC G^AATTC
EcoT22I 98 ATGCAT ATGCA^T
FauI 552 CCCGC CCCGC(4/6)
HindIII 2456 AAGCTT A^AGCTT
KpnI 224 GGTACC GGTAC^C
MamI 625 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3234 TGGCCA TGG^CCA
MscI 3234 TGGCCA TGG^CCA
MvnI 2011 CGCG CG^CG
NciI 1368 CCGGG CC^[GC]GG
NcoI 2273 CCATGG C^CATGG
NsiI 98 ATGCAT ATGCA^T
PflFI 1261 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2664 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1053 ATTAAT AT^TAAT
PstI 2788 CTGCAG CTGCA^G
PvuII 2105 CAGCTG CAG^CTG
SexAI 3192 ACCTGGT A^CC[AT]GGT
SnaBI 2864 TACGTA TAC^GTA
SphI 3429 GCATGC GCATG^C
SspBI 2300 TGTACA T^GTACA
SspI 856 AATATT AAT^ATT
Tth111I 1261 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1313 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 595 GTAGAC GT^[AC][GT]AC
708 GTATAC
AcyI 170 GACGCC G[GA]^CG[CT]C
602 GGCGTC
AlwNI 1762 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2663 CAGGTCCTG
AocI 781 CCTCAGG CC^T[ACGT]AGG
1691 CCTTAGG
BciVI 72 GGATAC GTATCC(6/5)
1995 GTATCC
BsaHI 170 GACGCC G[GA]^CG[CT]C
602 GGCGTC
BsaI 889 GAGACC GGTCTC(1/5)
1666 GAGACC
BsgI 268 CTGCAC GTGCAG(16/14)
1088 CTGCAC
BsmBI 2013 CGTCTC CGTCTC(1/5)
949 GAGACG
BsmFI 183 GGGAC GGGAC(10/14)
2342 GGGAC
BspHI 750 TCATGA T^CATGA
3452 TCATGA
BsrDI 2803 CATTGC GCAATG(2/0)
2913 CATTGC
BstXI 2258 CCAACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2810 CCATCTATGTGG
BstYI 454 GGATCT [GA]^GATC[CT]
2025 GGATCT
Bsu36I 781 CCTCAGG CC^T[ACGT]AGG
1691 CCTTAGG
EaeI 2077 TGGCCG [CT]^GGCC[GA]
3234 TGGCCA
Eco81I 781 CCTCAGG CC^T[ACGT]AGG
1691 CCTTAGG
Hin1I 170 GACGCC G[GA]^CG[CT]C
602 GGCGTC
MaeII 190 ACGT A^CGT
2865 ACGT
MflI 454 GGATCT [GA]^GATC[CT]
2025 GGATCT
MspA1I 1864 CCGCTG C[AC]G^C[GT]G
2105 CAGCTG
PflMI 1303 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3226 CCATCCAATGG
PshAI 598 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3151 GACTGCTGTC
RcaI 750 TCATGA T^CATGA
3452 TCATGA
TaqI 3312 TCGA T^CGA
3375 TCGA
TthHB8I 3312 TCGA T^CGA
3375 TCGA
Van91I 1303 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3226 CCATCCAATGG
XcmI 1790 CCACTATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3222 CCAGCCATCCAATGG
XhoII 454 GGATCT [GA]^GATC[CT]
2025 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmI BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI