Restriction map for
pFN21AA0966
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiWI BsoBI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrFI BsrGI Bst1107I BstEII BstPI
BstZ17I CelII Cfr10I CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I KasI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NotI NsiI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1132 GTAGAC GT^[AC][GT]AC
AflIII 2549 ACATGT A^C[GA][CT]GT
ApaI 1862 GGGCCC GGGCC^C
AseI 3328 ATTAAT AT^TAAT
AsnI 3328 ATTAAT AT^TAAT
Asp700I 501 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2687 CCTCAGC CCTCAGC(-5/-2)
BcnI 1464 CCCGG CC^[GC]GG
BsaAI 1948 TACGTG [CT]AC^GT[GA]
BsiEI 591 CGGTCG CG[GA][CT]^CG
BsmBI 1907 GAGACG CGTCTC(1/5)
BspDI 1016 ATCGAT AT^CGAT
BspLU11I 2549 ACATGT A^CATGT
BssSI 977 CACGAG CACGAG(-5/-1)
BstAPI 1179 GCAAGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1016 ATCGAT AT^CGAT
DrdI 238 GACCATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3028 CGGCCA [CT]^GGCC[GA]
EcoNI 33 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2584 GAATTC G^AATTC
FauI 94 CCCGC CCCGC(4/6)
HaeII 2691 AGCGCC [GA]GCGC^[CT]
HapII 1465 CCGG C^CGG
HincII 2437 GTTAAC GT[CT]^[GA]AC
HindII 2437 GTTAAC GT[CT]^[GA]AC
HindIII 1754 AAGCTT A^AGCTT
HpaI 2437 GTTAAC GTT^AAC
HpaII 1465 CCGG C^CGG
KspI 1033 CCGCGG CCGC^GG
MspI 1465 CCGG C^CGG
NciI 1464 CCCGG CC^[GC]GG
NheI 2622 GCTAGC G^CTAGC
NruI 1415 TCGCGA TCG^CGA
PflFI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3328 ATTAAT AT^TAAT
SacII 1033 CCGCGG CCGC^GG
SspI 2386 AATATT AAT^ATT
Tth111I 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2004 TCTAGA T^CTAGA
XmnI 501 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 668 GTAC GT^AC
2836 GTAC
AocI 295 CCTCAGG CC^T[ACGT]AGG
787 CCTCAGG
BglI 68 GCCGCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
597 GCCCCTCTGGC
BseRI 477 GAGGAG GAGGAG(10/8)
887 GAGGAG
BsrDI 2120 CATTGC GCAATG(2/0)
2175 CATTGC
BssHII 48 GCGCGC G^CGCGC
50 GCGCGC
BstBI 1084 TTCGAA TT^CGAA
3221 TTCGAA
Bsu36I 295 CCTCAGG CC^T[ACGT]AGG
787 CCTCAGG
DraII 1862 GGGCCCT [GA]G^G[ACGT]CC[CT]
2031 AGGCCCT
Eco81I 295 CCTCAGG CC^T[ACGT]AGG
787 CCTCAGG
EcoO109I 1862 GGGCCCT [GA]G^G[ACGT]CC[CT]
2031 AGGCCCT
NspV 1084 TTCGAA TT^CGAA
3221 TTCGAA
PstI 34 CTGCAG CTGCA^G
2188 CTGCAG
PvuII 1438 CAGCTG CAG^CTG
1556 CAGCTG
RsaI 668 GTAC GT^AC
2836 GTAC
SacI 4 GAGCTC GAGCT^C
313 GAGCTC
SapI 2250 GAAGAGC GCTCTTC(1/4)
6 GCTCTTC
SfuI 1084 TTCGAA TT^CGAA
3221 TTCGAA
SphI 2072 GCATGC GCATG^C
3134 GCATGC
XcmI 1412 CCATCGCGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2198 CCAAGCAAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII