Restriction map for
pFN21ASDB0002
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Aor51HI AseI AsnI Asp700I
AspEI AvrII BbeI BbrPI BfrI
BlnI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 317 GGTACC G^GTACC
AcsI 2698 GAATTT [GA]^AATT[CT]
AcyI 1952 GACGCC G[GA]^CG[CT]C
AflIII 2201 ACATGT A^C[GA][CT]GT
Alw44I 3582 GTGCAC G^TGCAC
ApaLI 3582 GTGCAC G^TGCAC
ApoI 2698 GAATTT [GA]^AATT[CT]
AscI 123 GGCGCGCC GG^CGCGCC
Asp718I 317 GGTACC G^GTACC
AspI 1614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2592 GGATCC G^GATCC
BbsI 1611 GAAGAC GAAGAC(2/6)
BciVI 2455 GGATAC GTATCC(6/5)
BclI 2709 TGATCA T^GATCA
BglII 1545 AGATCT A^GATCT
BlpI 1383 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1383 GCTAAGC GC^T[ACGT]AGC
BpuAI 1611 GAAGAC GAAGAC(2/6)
BsaAI 3001 TACGTG [CT]AC^GT[GA]
BsaHI 1952 GACGCC G[GA]^CG[CT]C
BsaI 751 GAGACC GGTCTC(1/5)
BsiEI 605 CGGCCG CG[GA][CT]^CG
BsmI 475 GCATTC GAATGC(1/-1)
BspLU11I 2201 ACATGT A^CATGT
CelII 1383 GCTAAGC GC^T[ACGT]AGC
EagI 605 CGGCCG C^GGCCG
EclXI 605 CGGCCG C^GGCCG
Eco52I 605 CGGCCG C^GGCCG
FbaI 2709 TGATCA T^GATCA
Hin1I 1952 GACGCC G[GA]^CG[CT]C
KpnI 317 GGTACC GGTAC^C
KspI 1368 CCGCGG CCGC^GG
NheI 2917 GCTAGC G^CTAGC
PaeR7I 130 CTCGAG C^TCGAG
PflFI 1614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 1368 CCGCGG CCGC^GG
ScaI 134 AGTACT AGT^ACT
SexAI 245 ACCTGGT A^CC[AT]GGT
SfiI 765 GGCCATGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 71 GCATGC GCATG^C
SspI 2692 AATATT AAT^ATT
Tsp509I 2699 AATT ^AATT
Tth111I 1614 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 130 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 2257 CCTGAGG CC^T[ACGT]AGG
3574 CCTCAGG
AviII 38 TGCGCA TGC^GCA
56 TGCGCA
BbvCI 3110 CCTCAGC CCTCAGC(-5/-2)
3450 GCTGAGG
BsaBI 537 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
624 GATGAACATC
BspMI 53 ACCTGC ACCTGC(4/8)
714 GCAGGT
BssHII 96 GCGCGC G^CGCGC
124 GCGCGC
Bsu36I 2257 CCTGAGG CC^T[ACGT]AGG
3574 CCTCAGG
DraIII 244 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3320 CACCCAGTG
Eco81I 2257 CCTGAGG CC^T[ACGT]AGG
3574 CCTCAGG
EcoNI 423 CCTTGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2179 CCTTGCACAGG
FspI 38 TGCGCA TGC^GCA
56 TGCGCA
MamI 537 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
624 GATGAACATC
NcoI 1197 CCATGG C^CATGG
3089 CCATGG
PflMI 1678 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1962 CCAGAGAGTGG
StuI 2929 AGGCCT AGG^CCT
3454 AGGCCT
TfiI 1342 GATTC G^A[AT]TC
2416 GATTC
Van91I 1678 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1962 CCAGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI