Restriction map for
pFN21ASDA1225
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# NO CUT ENZYMES ( 115 enzymes) #
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AatII AccII AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AspEI AspI AvaI AviII
AvrII BalI BbeI BbrPI BcgI
BcnI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI KspI MluI
MluNI MroI MscI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 33 GGTACC G^GTACC
AccI 47 GTCTAC GT^[AC][GT]AC
AclI 246 AACGTT AA^CGTT
Asp718I 33 GGTACC G^GTACC
BamHI 3861 GGATCC G^GATCC
BciVI 2436 GTATCC GTATCC(6/5)
BglI 3663 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2562 AGATCT A^GATCT
BsiEI 3186 CGACCG CG[GA][CT]^CG
BsmI 3540 GCATTC GAATGC(1/-1)
BspHI 2166 TCATGA T^CATGA
BspMI 2772 GCAGGT ACCTGC(4/8)
BssSI 3578 CTCGTG CACGAG(-5/-1)
BstAPI 256 GCATCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 2877 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 2967 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3808 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2877 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 583 GAATTC G^AATTC
EcoRV 1445 GATATC GAT^ATC
EcoT22I 3413 ATGCAT ATGCA^T
HpaI 1765 GTTAAC GTT^AAC
KpnI 33 GGTACC GGTAC^C
MaeII 247 ACGT A^CGT
NsiI 3413 ATGCAT ATGCA^T
Psp1406I 246 AACGTT AA^CGTT
RcaI 2166 TCATGA T^CATGA
SpeI 3499 ACTAGT A^CTAGT
StuI 3670 AGGCCT AGG^CCT
XbaI 3733 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 969 CTTAAG C^TTAAG
1471 CTTAAG
AflIII 751 ACATGT A^C[GA][CT]GT
1274 ACATGT
AlwNI 1721 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3248 CAGCCTCTG
AocI 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
BanI 33 GGTACC G^G[CT][GA]CC
1416 GGCACC
BanII 1370 GGGCTC G[GA]GC[CT]^C
3287 GGGCTC
BbvCI 2674 CCTCAGC CCTCAGC(-5/-2)
3019 CCTCAGC
BclI 987 TGATCA T^GATCA
2760 TGATCA
BfrI 969 CTTAAG C^TTAAG
1471 CTTAAG
BsaBI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATTCAAATC
BsgI 2900 CTGCAC GTGCAG(16/14)
3383 CTGCAC
Bsp1407I 1503 TGTACA T^GTACA
1671 TGTACA
BspLU11I 751 ACATGT A^CATGT
1274 ACATGT
BsrGI 1503 TGTACA T^GTACA
1671 TGTACA
Bsu36I 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
DraI 1437 TTTAAA TTT^AAA
2133 TTTAAA
Eco81I 3673 CCTCAGG CC^T[ACGT]AGG
3706 CCTCAGG
EcoT14I 140 CCTTGG C^C[AT][AT]GG
1291 CCAAGG
FbaI 987 TGATCA T^GATCA
2760 TGATCA
HindIII 935 AAGCTT A^AGCTT
2379 AAGCTT
MamI 160 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2839 GATTCAAATC
NspI 751 ACATGT [GA]CATG^[CT]
1274 ACATGT
SspBI 1503 TGTACA T^GTACA
1671 TGTACA
StyI 140 CCTTGG C^C[AT][AT]GG
1291 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PshBI PstI PvuII RsaI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI