Restriction map for
pFN21ASDA0821
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# NO CUT ENZYMES ( 63 enzymes) #
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AccIII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AviII
AvrII BciVI BfrI BlnI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRV EcoT22I FspI
HindIII HpaI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SfuI SgrAI SnaBI SspBI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 3535 GGTACC G^GTACC
AcsI 3443 GAATTC [GA]^AATT[CT]
AocI 4204 CCTGAGG CC^T[ACGT]AGG
Aor51HI 935 AGCGCT AGC^GCT
ApoI 3443 GAATTC [GA]^AATT[CT]
Asp718I 3535 GGTACC G^GTACC
BalI 2624 TGGCCA TGG^CCA
BcgI 1197 CGACCCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3848 TGATCA T^GATCA
BglII 2546 AGATCT A^GATCT
BlpI 2716 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2716 GCTGAGC GC^T[ACGT]AGC
BsaBI 3846 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 2159 GCAATG GCAATG(2/0)
Bsu36I 4204 CCTGAGG CC^T[ACGT]AGG
CelII 2716 GCTGAGC GC^T[ACGT]AGC
DrdI 456 GACACACGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4094 CGGCCG C^GGCCG
EclXI 4094 CGGCCG C^GGCCG
Eco47III 935 AGCGCT AGC^GCT
Eco52I 4094 CGGCCG C^GGCCG
Eco81I 4204 CCTGAGG CC^T[ACGT]AGG
EcoRI 3443 GAATTC G^AATTC
FbaI 3848 TGATCA T^GATCA
FseI 1469 GGCCGGCC GGCCGG^CC
KpnI 3535 GGTACC GGTAC^C
MamI 3846 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2624 TGGCCA TGG^CCA
MscI 2624 TGGCCA TGG^CCA
MseI 3137 TTAA T^TAA
NcoI 3539 CCATGG C^CATGG
NruI 1128 TCGCGA TCG^CGA
NspI 1487 GCATGC [GA]CATG^[CT]
PaeR7I 1520 CTCGAG C^TCGAG
PflMI 300 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 337 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1429 GAGCTC GAGCT^C
SalI 2448 GTCGAC G^TCGAC
SbfI 4308 CCTGCAGG CCTGCA^GG
ScaI 2891 AGTACT AGT^ACT
SfiI 2254 GGCCCGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2853 ACTAGT A^CTAGT
SphI 1487 GCATGC GCATG^C
Sse8387I 4308 CCTGCAGG CCTGCA^GG
Tru9I 3137 TTAA T^TAA
Van91I 300 CCAGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1520 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 6 enzymes) #
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Asp700I 405 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2385 GAACCACTTC
BamHI 536 GGATCC G^GATCC
3382 GGATCC
BsiEI 2670 CGACCG CG[GA][CT]^CG
4094 CGGCCG
DraIII 297 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1324 CACCCAGTG
SapI 1431 GCTCTTC GCTCTTC(1/4)
2724 GCTCTTC
XmnI 405 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2385 GAACCACTTC
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# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AspI
AvaI AvaII BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XcmI XhoII
XmaCI XmaI