Restriction map for
pFN21ASDA0142
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BalI BamHI BbeI BbrPI
BbsI BcgI BclI BfrI BglII
BpuAI BsaBI BsiWI Bsp1407I BspDI
BspHI BspMI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 91 GACGTC GACGT^C
AflIII 911 ACATGT A^C[GA][CT]GT
AvaI 724 CTCGAG C^[CT]CG[GA]G
AvrII 1009 CCTAGG C^CTAGG
BbvCI 1454 CCTCAGC CCTCAGC(-5/-2)
BciVI 1773 GGATAC GTATCC(6/5)
BglI 149 GCCGGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1009 CCTAGG C^CTAGG
BlpI 490 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 490 GCTCAGC GC^T[ACGT]AGC
BsgI 1626 CTGCAC GTGCAG(16/14)
BsiEI 147 CGGCCG CG[GA][CT]^CG
BsmBI 89 GAGACG CGTCTC(1/5)
BsmI 469 GAATGC GAATGC(1/-1)
BsoBI 724 CTCGAG C^[CT]CG[GA]G
BspLU11I 911 ACATGT A^CATGT
BsrBI 1126 GAGCGG CCGCTC(-3/-3)
BsrDI 365 CATTGC GCAATG(2/0)
BsrFI 154 ACCGGC [GA]^CCGG[CT]
BssSI 655 CTCGTG CACGAG(-5/-1)
BstAPI 1155 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 490 GCTCAGC GC^T[ACGT]AGC
Cfr10I 154 ACCGGC [GA]^CCGG[CT]
CpoI 151 CGGACCG CG^G[AT]CCG
DraI 516 TTTAAA TTT^AAA
EagI 147 CGGCCG C^GGCCG
EclXI 147 CGGCCG C^GGCCG
Eco52I 147 CGGCCG C^GGCCG
EcoNI 238 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 962 GATATC GAT^ATC
HaeII 1633 AGCGCC [GA]GCGC^[CT]
NcoI 776 CCATGG C^CATGG
NspI 911 ACATGT [GA]CATG^[CT]
PaeR7I 724 CTCGAG C^TCGAG
RsrII 151 CGGACCG CG^G[AT]CCG
SacI 721 GAGCTC GAGCT^C
SapI 231 GAAGAGC GCTCTTC(1/4)
TaqI 725 TCGA T^CGA
TthHB8I 725 TCGA T^CGA
XhoI 724 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccIII 818 TCCGGA T^CCGGA
863 TCCGGA
AcyI 91 GACGTC G[GA]^CG[CT]C
1314 GACGCC
AspHI 721 GAGCTC G[AT]GC[AT]^C
1177 GAGCAC
BanI 634 GGTGCC G^G[CT][GA]CC
1354 GGCACC
BcnI 1266 CCCGG CC^[GC]GG
1565 CCCGG
BsaAI 175 TACGTG [CT]AC^GT[GA]
552 CACGTA
BsaHI 91 GACGTC G[GA]^CG[CT]C
1314 GACGCC
BsaI 625 GAGACC GGTCTC(1/5)
1927 GAGACC
BsaWI 818 TCCGGA [AT]^CCGG[AT]
863 TCCGGA
BseAI 818 TCCGGA T^CCGGA
863 TCCGGA
BseRI 604 GAGGAG GAGGAG(10/8)
1552 GAGGAG
BsiHKAI 721 GAGCTC G[AT]GC[AT]^C
1177 GAGCAC
BspEI 818 TCCGGA T^CCGGA
863 TCCGGA
DraII 920 AGGTCCT [GA]G^G[ACGT]CC[CT]
1380 GGGACCC
EaeI 147 CGGCCG [CT]^GGCC[GA]
361 CGGCCA
EcoO109I 920 AGGTCCT [GA]G^G[ACGT]CC[CT]
1380 GGGACCC
EcoT14I 776 CCATGG C^C[AT][AT]GG
1009 CCTAGG
EcoT22I 1097 ATGCAT ATGCA^T
1784 ATGCAT
HgaI 1314 GACGC GACGC(5/10)
1561 GCGTC
Hin1I 91 GACGTC G[GA]^CG[CT]C
1314 GACGCC
MroI 818 TCCGGA T^CCGGA
863 TCCGGA
NciI 1266 CCCGG CC^[GC]GG
1565 CCCGG
NsiI 1097 ATGCAT ATGCA^T
1784 ATGCAT
PflMI 1370 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1406 CCAAGCCCTGG
PleI 500 GAGTC GAGTC(4/5)
1764 GAGTC
PpuMI 920 AGGTCCT [GA]G^G[AT]CC[CT]
1380 GGGACCC
PvuII 1457 CAGCTG CAG^CTG
1577 CAGCTG
SfaNI 525 GATGC GCATC(5/9)
1594 GATGC
SspI 298 AATATT AAT^ATT
320 AATATT
StyI 776 CCATGG C^C[AT][AT]GG
1009 CCTAGG
Van91I 1370 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1406 CCAAGCCCTGG
XbaI 420 TCTAGA T^CTAGA
1842 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII