Restriction map for
pFN21AA0092
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BglI
BglII BlnI BmyI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MluI MluNI
MroI MscI MslI MspI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
TspRI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 9 GGCGTC G[GA]^CG[CT]C
AfaI 319 GTAC GT^AC
AflII 217 CTTAAG C^TTAAG
AflIII 540 ACATGT A^C[GA][CT]GT
AlwI 567 GGATC GGATC(4/5)
AseI 988 ATTAAT AT^TAAT
AsnI 988 ATTAAT AT^TAAT
AvaII 1295 GGACC G^G[AT]CC
BfrI 217 CTTAAG C^TTAAG
BlpI 55 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
BsaHI 9 GGCGTC G[GA]^CG[CT]C
BsaI 1326 GAGACC GGTCTC(1/5)
BsaJI 827 CCGTGG C^C[ACGT][ACGT]GG
BsiYI 822 CCATGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 822 CCATGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1294 GGGAC GGGAC(10/14)
BsmI 90 GCATTC GAATGC(1/-1)
BspLU11I 540 ACATGT A^CATGT
BsrDI 887 GCAATG GCAATG(2/0)
BssSI 935 CACGAG CACGAG(-5/-1)
BstBI 1175 TTCGAA TT^CGAA
BstYI 567 GGATCT [GA]^GATC[CT]
CelII 55 GCTGAGC GC^T[ACGT]AGC
DraII 608 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 935 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 827 CCGTGG C^C[GA][CT]GG
EcoO109I 608 AGGCCCT [GA]G^G[ACGT]CC[CT]
HaeIII 609 GGCC GG^CC
Hin1I 9 GGCGTC G[GA]^CG[CT]C
HindIII 878 AAGCTT A^AGCTT
HphI 1053 TCACC GGTGA(8/7)
MaeIII 964 GTAAC ^GT[ACGT]AC
MflI 567 GGATCT [GA]^GATC[CT]
NdeI 104 CATATG CA^TATG
NlaIV 1294 GGGACC GG[ACGT]^[ACGT]CC
NspV 1175 TTCGAA TT^CGAA
PflMI 822 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 483 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 988 ATTAAT AT^TAAT
PstI 404 CTGCAG CTGCA^G
RsaI 319 GTAC GT^AC
ScaI 318 AGTACT AGT^ACT
SfaNI 855 GCATC GCATC(5/9)
SfcI 404 CTGCAG C^T[GA][CT]AG
SfuI 1175 TTCGAA TT^CGAA
Van91I 822 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 567 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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BclI 531 TGATCA T^GATCA
1050 TGATCA
BpmI 1195 CTGGAG CTGGAG(16/14)
1331 CTGGAG
BseRI 975 CTCCTC GAGGAG(10/8)
994 GAGGAG
BsmAI 1326 GAGAC GTCTC(1/5)
16 GTCTC
BsrI 321 ACTGG ACTGG(1/-1)
706 ACTGG
BssKI 1077 CCAGG ^CC[ACGT]GG
1330 CCTGG
BstNI 1077 CCAGG CC^[AT]GG
1330 CCTGG
Cfr13I 609 GGCCC G^G[ACGT]CC
1295 GGACC
EcoRI 348 GAATTC G^AATTC
1386 GAATTC
EcoRII 1077 CCAGG ^CC[AT]GG
1330 CCTGG
FbaI 531 TGATCA T^GATCA
1050 TGATCA
MfeI 302 CAATTG C^AATTG
436 CAATTG
MspA1I 692 CAGCTG C[AC]G^C[GT]G
1192 CAGCTG
MunI 302 CAATTG C^AATTG
436 CAATTG
MvaI 1077 CCAGG CC^[AT]GG
1330 CCTGG
PvuII 692 CAGCTG CAG^CTG
1192 CAGCTG
Sau96I 609 GGCCC G^G[ACGT]CC
1295 GGACC
ScrFI 1077 CCAGG CC^[ACGT]GG
1330 CCTGG
SmlI 217 CTTAAG C^T[CT][GA]AG
253 CTTGAG
SphI 465 GCATGC GCATG^C
677 GCATGC
XbaI 292 TCTAGA T^CTAGA
711 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BstF5I Cac8I DdeI DpnII
Fnu4HI FokI HgaI HinfI ItaI
MaeI MboI MboII MnlI MseI
MwoI NdeII NlaIII NspI PleI
Sau3AI TaqI TfiI Tru9I TseI
Tsp509I TthHB8I