Restriction map for
pFN21ASDF0380
(Circular) (Six-base) MAPSORT of: pFN21ASDF0380.seq Check: 4517 from: 1 to: 7045
pFN21ASDF0380 7045 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2819 2819
Size: 7045
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4855 278
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4388 1937
Size: 2451 4594
Acc65I G'GTAC_C
Cuts at: 4366 4366
Size: 7045
AclI AA'CG_TT
Cuts at: 1563 5172 5545 1563
Size: 3609 373 3063
Fragments arranged by size:
3609 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 3139 3522 5108 6492 6760 1632
Size: 422 1085 383 1586 1384 268 1917
Fragments arranged by size:
1917 1586 1384 1085 422 383 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6848
AflIII A'CryG_T
Cuts at: 1203 5944 6921 1203
Size: 4741 977 1327
Fragments arranged by size:
4741 1327 977
AgeI A'CCGG_T
Cuts at: 5876 5876
Size: 7045
AhdI GACnn_n'nnGTC
Cuts at: 5774 5774
Size: 7045
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7045
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7045
AlwNI CAG_nnn'CTG
Cuts at: 4171 4788 6360 4171
Size: 617 1572 4856
Fragments arranged by size:
4856 1572 617
ApaI G_GGCC'C
Cuts at: 2160 2290 2160
Size: 130 6915
ApaLI G'TGCA_C
Cuts at: 5102 6258 5102
Size: 1156 5889
ApoI r'AATT_y
Cuts at: 1493 3437 4354 4580 4732 6841 1493
Size: 1944 917 226 152 2109 1697
Fragments arranged by size:
2109 1944 1697 917 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5599 160
Size: 872 4567 1606
Fragments arranged by size:
4567 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
AvaI C'yCGr_G
Cuts at: 1870 1943 2393 3161 3876 4370 1870
Size: 73 450 768 715 494 4545
Fragments arranged by size:
4545 768 715 494 450 73
BamHI G'GATC_C
Cuts at: 3694 4375 4742 3694
Size: 681 367 5997
Fragments arranged by size:
5997 681 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2305 2746 3760 3999 4366
Size: 325 217 627 518 441 1014 239 367
Cuts at: 4366 5821 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1014 627 518 441 367 325
239 217
BanII G_rGCy'C
Cuts at: 1391 2160 2290 2889 3270 3877 4364 1391
Size: 769 130 599 381 607 487 4072
Fragments arranged by size:
4072 769 607 599 487 381 130
BbeI G_GCGC'C
Cuts at: 4003 4003
Size: 7045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6074
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5236 5270 1903
Size: 34 3299 34 3678
Fragments arranged by size:
3678 3299 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2101 2231 4937 6147 2101
Size: 130 2706 1210 2999
Fragments arranged by size:
2999 2706 1210 130
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7045
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5656 136
Size: 107 122 71 631 605 3984 1525
Fragments arranged by size:
3984 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2539 2968 7040 2539
Size: 429 4072 2544
Fragments arranged by size:
4072 2544 429
BlpI GC'TnA_GC
Cuts at: 3702 3977 4462 3702
Size: 275 485 6285
Fragments arranged by size:
6285 485 275
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2290 3040 3772 3805 5106 6262 1790
Size: 370 130 750 732 33 1301 1156 2573
Fragments arranged by size:
2573 1301 1156 750 732 370 130 33
BmgBI CAC'GTC
Cuts at: 1318 2104 1318
Size: 786 6259
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2154 2176 2501
Size: 416 53 83 399 24 737 22 325
Cuts at: 2501 2733 3759 3792 4221 4776 5734 442
Size: 232 1026 33 429 555 958 1753
Fragments arranged by size:
1753 1026 958 737 555 429 416 399
325 232 83 53 33 24 22
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7045
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 3066 3449 3454 3746 4088 4226 5705
Size: 806 1021 383 5 292 342 138 1479
Cuts at: 5705 1239
Size: 2579
Fragments arranged by size:
2579 1479 1021 806 383 342 292 138
5
Bpu10I CC'TnA_GC
Cuts at: 3491 3491
Size: 7045
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3791 5170 6035 6333 3791
Size: 1379 865 298 4503
Fragments arranged by size:
4503 1379 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2337 3042 3608 5708 882
Size: 617 838 705 566 2100 2219
Fragments arranged by size:
2219 2100 838 705 617 566
BsaAI yAC'GTr
Cuts at: 493 1204 3733 4342 4836 493
Size: 711 2529 609 494 2702
Fragments arranged by size:
2702 2529 711 609 494
BsaBI GATnn'nnATC
Cuts at: 2549 2571 7039 2549
Size: 22 4468 2555
Fragments arranged by size:
4468 2555 22
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3119 4000 4852 5234
Size: 53 83 186 936 1586 881 852 382
Cuts at: 5234 5869 275
Size: 635 1451
Fragments arranged by size:
1586 1451 936 881 852 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4329 5478 5876 6150 6297 6708 4329
Size: 1149 398 274 147 411 4666
Fragments arranged by size:
4666 1149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6820
Fragments arranged by size:
6820 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2907 3446 3517 3520 3944 4259 1186
Size: 1075 646 539 71 3 424 315 3972
Fragments arranged by size:
3972 1075 646 539 424 315 71 3
BseYI C'CCAG_C
Cuts at: 2582 3215 3282 3423 3972 6248 6744 2582
Size: 633 67 141 549 2276 496 2883
Fragments arranged by size:
2883 2276 633 549 496 141 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2724 3434 1163
Size: 573 988 710 4774
Fragments arranged by size:
4774 988 710 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4495 5256 6284 1540
Size: 275 186 2494 761 1028 2301
Fragments arranged by size:
2494 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3062 3732 3870 5106 5191 6262 3062
Size: 670 138 1236 85 1071 3845
Fragments arranged by size:
3845 1236 1071 670 138 85
BsmI GAATG_Cn'
Cuts at: 2833 4556 4649 2833
Size: 1723 93 5229
Fragments arranged by size:
5229 1723 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2290 2889 3040 3062 3270 3732
Size: 399 370 130 599 151 22 208 462
Cuts at: 3732 3772 3805 3870 3877 4364 5106 5191 6262
Size: 40 33 65 7 487 742 85 1071
Cuts at: 6262 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 599 487 462 399 370
208 151 130 85 65 40 33 22
7
BspEI T'CCGG_A
Cuts at: 6708 6708
Size: 7045
BspHI T'CATG_A
Cuts at: 4934 6664 4934
Size: 1730 5315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2712 2819 3795 3927 4095 4400 844
Size: 1868 107 976 132 168 305 3489
Fragments arranged by size:
3489 1868 976 305 168 132 107
BsrBI CCG'CTC
Cuts at: 1394 1823 4932 1394
Size: 429 3109 3507
Fragments arranged by size:
3507 3109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5540 5714 65
Size: 1183 4292 174 1396
Fragments arranged by size:
4292 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5689 5876 1700
Size: 110 3879 187 2869
Fragments arranged by size:
3879 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3030 96
Size: 2934 4111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7045
BssSI C'ACGA_G
Cuts at: 5105 6117 5105
Size: 1012 6033
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3767 794
Size: 2973 4072
BstBI TT'CG_AA
Cuts at: 2007 5849 2007
Size: 3842 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7045
BstXI CCAn_nnnn'nTGG
Cuts at: 3290 3772 3290
Size: 482 6563
BstYI r'GATC_y
Cuts at: 1972 2539 2968 3622 3694 3888 4375 4742 5127
Size: 567 429 654 72 194 487 367 385
Cuts at: 5127 5144 6596 6682 6694 7040 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 654 567 487 429 385 367
346 194 86 72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2195 2792 2195
Size: 597 6448
BtgI C'CryG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 4561
Fragments arranged by size:
4561 1932 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6306
Fragments arranged by size:
6306 683 56
BtsI GCAGTG_nn'
Cuts at: 2256 2874 2966 4267 4561 5355 5375 2256
Size: 618 92 1301 294 794 20 3926
Fragments arranged by size:
3926 1301 794 618 294 92 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7010
DraI TTT'AAA
Cuts at: 4349 4349
Size: 7045
DraIII CAC_nnn'GTG
Cuts at: 3767 3800 3767
Size: 33 7012
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2773 4262 6052 809
Size: 507 1457 1489 1790 1802
Fragments arranged by size:
1802 1790 1489 1457 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3183 3387 4287
Size: 54 1425 50 275 18 1353 204 900
Cuts at: 4287 4492 5381 7010 8
Size: 205 889 1629 43
Fragments arranged by size:
1629 1425 1353 900 889 275 205 204
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4492 1537
Size: 275 2680 4090
Fragments arranged by size:
4090 2680 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2778 2901 2925 3158 3275 3523 3538 3544
Size: 1425 123 24 233 117 248 15 6
Cuts at: 3544 3571 3624 4975 1353
Size: 27 53 1351 3423
Fragments arranged by size:
3423 1425 1351 248 233 123 117 53
27 24 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5632 6006 6152 387
Size: 1064 138 4043 374 146 1280
Fragments arranged by size:
4043 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2054 3066 3139 3449 3454 3522
Size: 393 413 9 1012 73 310 5 68
Cuts at: 3522 3746 4088 4226 5108 5705 6492 6760 1239
Size: 224 342 138 882 597 787 268 1524
Fragments arranged by size:
1524 1012 882 787 597 413 393 342
310 268 224 138 73 68 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 3113 4175 2276
Size: 837 1062 5146
Fragments arranged by size:
5146 1062 837
EcoO109I rG'GnC_Cy
Cuts at: 2156 2286 2287 4084 2156
Size: 130 1 1797 5117
Fragments arranged by size:
5117 1797 130 1
EcoRI G'AATT_C
Cuts at: 4354 4732 4354
Size: 378 6667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2807 2059
Size: 748 6297
FspI TGC'GCA
Cuts at: 5551 5551
Size: 7045
HaeII r_GCGC'y
Cuts at: 1678 4003 6192 1678
Size: 2325 2189 2531
Fragments arranged by size:
2531 2325 2189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2960 2992 3434 3466 3607
Size: 32 135 32 1388 32 442 32 141
Cuts at: 3607 3639 5686 5718 5760 5792 1373
Size: 32 2047 32 42 32 2626
Fragments arranged by size:
2626 2047 1388 442 141 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4389 4635 669
Size: 988 281 2451 246 3079
Fragments arranged by size:
3079 2451 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7045
HpaI GTT'AAC
Cuts at: 4635 4635
Size: 7045
KasI G'GCGC_C
Cuts at: 3999 3999
Size: 7045
KpnI G_GTAC'C
Cuts at: 4370 4370
Size: 7045
MfeI C'AATT_G
Cuts at: 4644 4644
Size: 7045
MluI A'CGCG_T
Cuts at: 6921 6921
Size: 7045
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3342 6159 6343 1305
Size: 2037 2817 184 2007
Fragments arranged by size:
2817 2037 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3185 3389 10
Size: 54 1425 343 1353 204 3666
Fragments arranged by size:
3666 1425 1353 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2987 3002 5003 5362 5521 518
Size: 648 1821 15 2001 359 159 2042
Fragments arranged by size:
2042 2001 1821 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 3427 3976 4461 5138 6286 6531 1606
Size: 1821 549 485 677 1148 245 2120
Fragments arranged by size:
2120 1821 1148 677 549 485 245
NarI GG'CG_CC
Cuts at: 4000 4000
Size: 7045
NcoI C'CATG_G
Cuts at: 513 1065 2997 513
Size: 552 1932 4561
Fragments arranged by size:
4561 1932 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7045
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7045
NotI GC'GGCC_GC
Cuts at: 4492 4492
Size: 7045
NspI r_CATG'y
Cuts at: 1132 1229 4403 5948 1132
Size: 97 3174 1545 2229
Fragments arranged by size:
3174 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3124 1348
Size: 1776 5269
PciI A'CATG_T
Cuts at: 5944 5944
Size: 7045
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2513 1108
Size: 1405 5640
PfoI T'CCnGG_A
Cuts at: 2139 3043 3840 2139
Size: 904 797 5344
Fragments arranged by size:
5344 904 797
PmeI GTTT'AAAC
Cuts at: 4349 4349
Size: 7045
PmlI CAC'GTG
Cuts at: 3733 3733
Size: 7045
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5114 5146 6627 6659 5114
Size: 32 1481 32 5500
Fragments arranged by size:
5500 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4084 4084
Size: 7045
PshAI GACnn'nnGTC
Cuts at: 1613 2604 1613
Size: 991 6054
PsiI TTA'TAA
Cuts at: 4615 4615
Size: 7045
PspOMI G'GGCC_C
Cuts at: 2156 2286 2156
Size: 130 6915
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 7013
PstI C_TGCA'G
Cuts at: 830 3137 4092 4397 830
Size: 2307 955 305 3478
Fragments arranged by size:
3478 2307 955 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7045
PvuII CAG'CTG
Cuts at: 3427 3976 3427
Size: 549 6496
SalI G'TCGA_C
Cuts at: 1936 4387 1936
Size: 2451 4594
SapI GCTCTTCn'nnn_
Cuts at: 2901 3275 2901
Size: 374 6671
SbfI CC_TGCA'GG
Cuts at: 4397 4397
Size: 7045
ScaI AGT'ACT
Cuts at: 5293 7005 5293
Size: 1712 5333
SfcI C'TryA_G
Cuts at: 826 1045 2496 2602 3067 3133 3598 4088 4393
Size: 219 1451 106 465 66 465 490 305
Cuts at: 4393 5528 6209 6400 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1451 1135 681 490 465 465 305
219 191 106 66
SfoI GGC'GCC
Cuts at: 4001 4001
Size: 7045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7045
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7045
SmaI CCC'GGG
Cuts at: 1872 4372 1872
Size: 2500 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2393 3806 5149 6050 6312 820
Size: 197 926 450 1413 1343 901 262 1553
Fragments arranged by size:
1553 1413 1343 926 901 450 262 197
SnaBI TAC'GTA
Cuts at: 493 4342 4836 493
Size: 3849 494 2702
Fragments arranged by size:
3849 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7045
SphI G_CATG'C
Cuts at: 1132 4403 1132
Size: 3271 3774
SspI AAT'ATT
Cuts at: 5 52 4969 5
Size: 47 4917 2081
Fragments arranged by size:
4917 2081 47
StuI AGG'CCT
Cuts at: 1517 2385 3419 3456 1517
Size: 868 1034 37 5106
Fragments arranged by size:
5106 1034 868 37
StyI C'CwwG_G
Cuts at: 513 1065 2205 2997 4484 513
Size: 552 1140 792 1487 3074
Fragments arranged by size:
3074 1487 1140 792 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3778 3811 5072 5089 5242 5427 5872
Size: 1255 2198 33 1261 17 153 185 445
Cuts at: 5872 325
Size: 1498
Fragments arranged by size:
2198 1498 1261 1255 445 185 153 33
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 5291 7003
Size: 274 80 33 51 1443 1053 2261 1712
Cuts at: 7003 96
Size: 138
Fragments arranged by size:
2261 1712 1443 1053 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2945 2977 7005 7037 2945
Size: 32 4028 32 2953
Fragments arranged by size:
4028 2953 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2935 1316
Size: 1619 5426
XbaI T'CTAG_A
Cuts at: 4381 4381
Size: 7045
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4063 4184 4063
Size: 121 6924
XhoI C'TCGA_G
Cuts at: 1943 2393 1943
Size: 450 6595
XmaI C'CCGG_G
Cuts at: 1870 4370 1870
Size: 2500 4545
XmnI GAAnn'nnTTC
Cuts at: 2055 5174 2055
Size: 3119 3926
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4853 276
Size: 53 83 186 936 3319 2468
Fragments arranged by size:
3319 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI