Restriction map for
pFN21ASDF0261
(Circular) (Six-base) MAPSORT of: pFN21ASDF0261.seq Check: 6023 from: 1 to: 9520
pFN21ASDF0261 9520 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3004 3619 6285 6706 3004
Size: 615 2666 421 5818
Fragments arranged by size:
5818 2666 615 421
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5706 7330 278
Size: 53 83 186 936 4170 1624 2468
Fragments arranged by size:
4170 2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5811 6863 1937
Size: 3874 1052 4594
Fragments arranged by size:
4594 3874 1052
Acc65I G'GTAC_C
Cuts at: 6841 6841
Size: 9520
AclI AA'CG_TT
Cuts at: 1563 7647 8020 1563
Size: 6084 373 3063
Fragments arranged by size:
6084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2526 3612 4049 4301 5157 6026 6779 7583
Size: 894 1086 437 252 856 869 753 804
Cuts at: 7583 8967 9235 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1086 894 869 856 804 753
437 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9323
AflIII A'CryG_T
Cuts at: 1203 6263 8419 9396 1203
Size: 5060 2156 977 1327
Fragments arranged by size:
5060 2156 1327 977
AgeI A'CCGG_T
Cuts at: 8351 8351
Size: 9520
AhdI GACnn_n'nnGTC
Cuts at: 8249 8249
Size: 9520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5531 1184
Size: 4347 5173
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 9488
AlwNI CAG_nnn'CTG
Cuts at: 3686 5140 5222 5365 5863 6757 7263 8835 3686
Size: 1454 82 143 498 894 506 1572 4371
Fragments arranged by size:
4371 1572 1454 894 506 498 143 82
ApaLI G'TGCA_C
Cuts at: 6747 7577 8733 6747
Size: 830 1156 7534
Fragments arranged by size:
7534 1156 830
ApoI r'AATT_y
Cuts at: 1493 2078 2474 5558 6829 7055 7207 9316 1493
Size: 585 396 3084 1271 226 152 2109 1697
Fragments arranged by size:
3084 2109 1697 1271 585 396 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8074 160
Size: 872 7042 1606
Fragments arranged by size:
7042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9520
AvaI C'yCGr_G
Cuts at: 1870 1943 3320 4617 5387 6315 6591 6845 1870
Size: 73 1377 1297 770 928 276 254 4545
Fragments arranged by size:
4545 1377 1297 928 770 276 254 73
AvrII C'CTAG_G
Cuts at: 4312 4312
Size: 9520
BamHI G'GATC_C
Cuts at: 3482 4182 6850 7217 3482
Size: 700 2668 367 5785
Fragments arranged by size:
5785 2668 700 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3016 3127 3293 3812 6446
Size: 325 217 627 1229 111 166 519 2634
Cuts at: 6446 6841 8296 618
Size: 395 1455 1842
Fragments arranged by size:
2634 1842 1455 1229 627 519 395 325
217 166 111
BanII G_rGCy'C
Cuts at: 1391 2286 2394 2685 2901 3387 3425 4857 5171
Size: 895 108 291 216 486 38 1432 314
Cuts at: 5171 6839 1391
Size: 1668 4072
Fragments arranged by size:
4072 1668 1432 895 486 314 291 216
108 38
BbeI G_GCGC'C
Cuts at: 6450 6450
Size: 9520
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 5541 928
Size: 971 3642 4907
Fragments arranged by size:
4907 3642 971
BbvCI CC'TCA_GC
Cuts at: 2031 2307 3345 3491 4941 2031
Size: 276 1038 146 1450 6610
Fragments arranged by size:
6610 1450 1038 276 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7711 7745 1903
Size: 34 5774 34 3678
Fragments arranged by size:
5774 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7412 8622 7412
Size: 1210 8310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 3922 5044
Size: 107 122 71 631 605 686 1564 1122
Cuts at: 5044 5136 6050 8131 136
Size: 92 914 2081 1525
Fragments arranged by size:
2081 1564 1525 1122 914 686 631 605
122 107 92 71
BglII A'GATC_T
Cuts at: 9515 9515
Size: 9520
BlpI GC'TnA_GC
Cuts at: 6937 6937
Size: 9520
Bme1580I G_kGCm'C
Cuts at: 1790 3697 6751 7581 8737 1790
Size: 1907 3054 830 1156 2573
Fragments arranged by size:
3054 2573 1907 1156 830
BmgBI CAC'GTC
Cuts at: 1318 6628 1318
Size: 5310 4210
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2341 2913 3428
Size: 416 53 83 399 24 924 572 515
Cuts at: 3428 3576 3629 7251 8209 442
Size: 148 53 3622 958 1753
Fragments arranged by size:
3622 1753 958 924 572 515 416 399
148 83 53 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2979 2554
Size: 425 9095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 3663 3855 4841 5039 5146 5387 5449
Size: 1493 931 192 986 198 107 241 62
Cuts at: 5449 5927 5978 6086 6563 6635 6686 8180 1239
Size: 478 51 108 477 72 51 1494 2579
Fragments arranged by size:
2579 1494 1493 986 931 478 477 241
198 192 108 107 72 62 51 51
Bpu10I CC'TnA_GC
Cuts at: 2031 2307 2964 3345 3491 4425 4941 6134 2031
Size: 276 657 381 146 934 516 1193 5417
Fragments arranged by size:
5417 1193 934 657 516 381 276 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4026 4504 5924 7645 8510 8808 4026
Size: 478 1420 1721 865 298 4738
Fragments arranged by size:
4738 1721 1420 865 478 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2125 2591 4060 4105 4894 5431 8183
Size: 617 626 466 1469 45 789 537 2752
Cuts at: 8183 882
Size: 2219
Fragments arranged by size:
2752 2219 1469 789 626 617 537 466
45
BsaAI yAC'GTr
Cuts at: 493 1204 3674 6817 7311 493
Size: 711 2470 3143 494 2702
Fragments arranged by size:
3143 2702 2470 711 494
BsaBI GATnn'nnATC
Cuts at: 9514 9514
Size: 9520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5027 5703 6447 7327
Size: 53 83 186 936 3494 676 744 880
Cuts at: 7327 7709 8344 275
Size: 382 635 1451
Fragments arranged by size:
3494 1451 936 880 744 676 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2323 4849 7953 8351 8625 8772 9183 2323
Size: 2526 3104 398 274 147 411 2660
Fragments arranged by size:
3104 2660 2526 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2365 2395 3769 3799 4117
Size: 30 165 30 962 30 1374 30 318
Cuts at: 4117 4147 5012 5042 5360 5390 5976 6006 1178
Size: 30 865 30 318 30 586 30 4692
Fragments arranged by size:
4692 1374 962 865 586 318 318 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2046 2048 2122 2480 2675 2729 3217 3230
Size: 860 2 74 358 195 54 488 13
Cuts at: 3230 3261 3452 3543 3567 4194 4231 4290 4618
Size: 31 191 91 24 627 37 59 328
Cuts at: 4618 5287 5419 6016 6047 6134 6349 6542 1186
Size: 669 132 597 31 87 215 193 4164
Fragments arranged by size:
4164 860 669 627 597 488 358 328
215 195 193 191 132 91 87 74
59 54 37 31 31 24 13 2
BseYI C'CCAG_C
Cuts at: 2040 3181 3796 4070 4082 5003 5301 8723 9219
Size: 1141 615 274 12 921 298 3422 496
Cuts at: 9219 2040
Size: 2341
Fragments arranged by size:
3422 2341 1141 921 615 496 298 274
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3654 4997 6263 6731 1163
Size: 573 1918 1343 1266 468 3952
Fragments arranged by size:
3952 1918 1343 1266 573 468
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6970 7731 8759 1540
Size: 275 186 4969 761 1028 2301
Fragments arranged by size:
4969 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 3398 3679 4616 4857 5667 6751 7581 7666
Size: 1004 281 937 241 810 1084 830 85
Cuts at: 7666 8737 2394
Size: 1071 3177
Fragments arranged by size:
3177 1084 1071 1004 937 830 810 281
241 85
BsmI GAATG_Cn'
Cuts at: 7031 7124 7031
Size: 93 9427
BsmBI CGTCTCn'nnnn_
Cuts at: 2234 5694 2234
Size: 3460 6060
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2286 2394 2685 2901 3387 3398 3425
Size: 399 496 108 291 216 486 11 27
Cuts at: 3425 3679 3697 4616 4857 5171 5667 6751 6839
Size: 254 18 919 241 314 496 1084 88
Cuts at: 6839 7581 7666 8737 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1084 1071 919 742 496 496 486
399 314 291 254 241 216 108 88
85 27 18 11
BspEI T'CCGG_A
Cuts at: 2323 4849 9183 2323
Size: 2526 4334 2660
Fragments arranged by size:
4334 2660 2526
BspHI T'CATG_A
Cuts at: 3306 7409 9139 3306
Size: 4103 1730 3687
Fragments arranged by size:
4103 3687 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 3004 3619 3943 4669 5451 5598 5823
Size: 1959 201 615 324 726 782 147 225
Cuts at: 5823 6285 6706 6875 844
Size: 462 421 169 3489
Fragments arranged by size:
3489 1959 782 726 615 462 421 324
225 201 169 147
BsrBI CCG'CTC
Cuts at: 1394 1823 3248 6172 7407 1394
Size: 429 1425 2924 1235 3507
Fragments arranged by size:
3507 2924 1425 1235 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 8015 8189 65
Size: 1183 3332 3435 174 1396
Fragments arranged by size:
3435 3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5130 8164 8351 1700
Size: 110 3320 3034 187 2869
Fragments arranged by size:
3320 3034 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9520
BssSI C'ACGA_G
Cuts at: 4260 7580 8592 4260
Size: 3320 1012 5188
Fragments arranged by size:
5188 3320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5140 794
Size: 4346 5174
BstBI TT'CG_AA
Cuts at: 2007 8324 2007
Size: 6317 3203
BstEII G'GTnAC_C
Cuts at: 1184 4968 1184
Size: 3784 5736
BstXI CCAn_nnnn'nTGG
Cuts at: 2394 2449 3165 2394
Size: 55 716 8749
Fragments arranged by size:
8749 716 55
BstYI r'GATC_y
Cuts at: 1972 2507 3482 4182 4524 5346 6501 6850 7217
Size: 535 975 700 342 822 1155 349 367
Cuts at: 7217 7602 7619 9071 9157 9169 9515 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1155 975 822 700 535 385
367 349 346 342 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2094 2658 3261 4902 5163 2094
Size: 564 603 1641 261 6451
Fragments arranged by size:
6451 1641 603 564 261
BtgI C'CryG_G
Cuts at: 513 1065 2022 4167 4236 5193 5402 5457 5493
Size: 552 957 2145 69 957 209 55 36
Cuts at: 5493 513
Size: 4540
Fragments arranged by size:
4540 2145 957 957 552 209 69 55
36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8781
Fragments arranged by size:
8781 683 56
BtsI GCAGTG_nn'
Cuts at: 3195 5083 6658 7036 7830 7850 3195
Size: 1888 1575 378 794 20 4865
Fragments arranged by size:
4865 1888 1575 794 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4563 4598 529
Size: 35 3999 35 5451
Fragments arranged by size:
5451 3999 35 35
DraI TTT'AAA
Cuts at: 6824 6824
Size: 9520
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3439 4074 4665 4679 8527 809
Size: 507 2123 635 591 14 3848 1802
Fragments arranged by size:
3848 2123 1802 635 591 507 14
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2480 4252 4374
Size: 54 1425 50 275 18 650 1772 122
Cuts at: 4374 4722 6513 6967 7856 9485 8
Size: 348 1791 454 889 1629 43
Fragments arranged by size:
1791 1772 1629 1425 889 650 454 348
275 122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6967 1537
Size: 275 5155 4090
Fragments arranged by size:
5155 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 2523 2545 2577 2640 2988 2996 3018
Size: 1104 66 22 32 63 348 8 22
Cuts at: 3018 3190 3207 4831 5176 5681 5727 6002 6290
Size: 172 17 1624 345 505 46 275 288
Cuts at: 6290 6470 7450 1353
Size: 180 980 3423
Fragments arranged by size:
3423 1624 1104 980 505 348 345 288
275 180 172 66 63 46 32 22
22 17 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3050 5792 8107 8481 8627 387
Size: 1064 138 1461 2742 2315 374 146 1280
Fragments arranged by size:
2742 2315 1461 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 4855 2392
Size: 2463 7057
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2526 2732 3612 3663 3855 4049 4301
Size: 393 894 206 880 51 192 194 252
Cuts at: 4301 4841 5039 5146 5157 5387 5449 5927 5978
Size: 540 198 107 11 230 62 478 51
Cuts at: 5978 6026 6086 6563 6635 6686 6779 7583 8180
Size: 48 60 477 72 51 93 804 597
Cuts at: 8180 8967 9235 1239
Size: 787 268 1524
Fragments arranged by size:
1524 894 880 804 787 597 540 478
477 393 268 252 230 206 198 194
192 107 93 72 62 60 51 51
51 48 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3646 4944 5021 5339 5480 3646
Size: 1298 77 318 141 7686
Fragments arranged by size:
7686 1298 318 141 77
EcoO109I rG'GnC_Cy
Cuts at: 2155 3630 3792 3924 4862 4906 5137 5210 5419
Size: 1475 162 132 938 44 231 73 209
Cuts at: 5419 2155
Size: 6256
Fragments arranged by size:
6256 1475 938 231 209 162 132 73
44
EcoRI G'AATT_C
Cuts at: 2078 6829 7207 2078
Size: 4751 378 4391
Fragments arranged by size:
4751 4391 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4364 4680 4364
Size: 316 9204
FspI TGC'GCA
Cuts at: 5144 5501 5594 8026 5144
Size: 357 93 2432 6638
Fragments arranged by size:
6638 2432 357 93
HaeII r_GCGC'y
Cuts at: 1678 5712 5932 6450 8667 1678
Size: 4034 220 518 2217 2531
Fragments arranged by size:
4034 2531 2217 518 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 2365 2397 2404
Size: 32 135 32 572 32 189 32 7
Cuts at: 2404 2436 3338 3370 3701 3733 3769 3801 4924
Size: 32 902 32 331 32 36 32 1123
Cuts at: 4924 4956 5012 5044 5139 5171 5360 5392 6625
Size: 32 56 32 95 32 189 32 1233
Cuts at: 6625 6657 8161 8193 8235 8267 1373
Size: 32 1504 32 42 32 2626
Fragments arranged by size:
2626 1504 1233 1123 902 572 331 189
189 135 95 56 42 36 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 7
HincII GTy'rAC
Cuts at: 669 1657 1938 6864 7110 669
Size: 988 281 4926 246 3079
Fragments arranged by size:
4926 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6491 748
Size: 5743 3777
HpaI GTT'AAC
Cuts at: 7110 7110
Size: 9520
KasI G'GCGC_C
Cuts at: 6446 6446
Size: 9520
KpnI G_GTAC'C
Cuts at: 6845 6845
Size: 9520
MfeI C'AATT_G
Cuts at: 7119 7119
Size: 9520
MluI A'CGCG_T
Cuts at: 6263 9396 6263
Size: 3133 6387
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5647 5679 5989 6442 6513 6756 8634 8818
Size: 4342 32 310 453 71 243 1878 184
Cuts at: 8818 1305
Size: 2007
Fragments arranged by size:
4342 2007 1878 453 310 243 184 71
32
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2482 4254 4376 6515 10
Size: 54 1425 343 650 1772 122 2139 3015
Fragments arranged by size:
3015 2139 1772 1425 650 343 122 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4260 5609 7478 7837 7996 518
Size: 648 3094 1349 1869 359 159 2042
Fragments arranged by size:
3094 2042 1869 1349 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3138 4370 5034 5305 5362 5404 5449 5469
Size: 1532 1232 664 271 57 42 45 20
Cuts at: 5469 6073 6796 6936 7613 8761 9006 1606
Size: 604 723 140 677 1148 245 2120
Fragments arranged by size:
2120 1532 1232 1148 723 677 664 604
271 245 140 57 45 42 20
NaeI GCC'GGC
Cuts at: 5132 5132
Size: 9520
NarI GG'CG_CC
Cuts at: 6447 6447
Size: 9520
NcoI C'CATG_G
Cuts at: 513 1065 2022 4167 5457 513
Size: 552 957 2145 1290 4576
Fragments arranged by size:
4576 2145 1290 957 552
NdeI CA'TA_TG
Cuts at: 387 4629 387
Size: 4242 5278
NgoMIV G'CCGG_C
Cuts at: 5130 5130
Size: 9520
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9520
NotI GC'GGCC_GC
Cuts at: 6967 6967
Size: 9520
NspI r_CATG'y
Cuts at: 1132 1229 4331 6878 8423 1132
Size: 97 3102 2547 1545 2229
Fragments arranged by size:
3102 2547 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4095 4961 6239 1348
Size: 2747 866 1278 4629
Fragments arranged by size:
4629 2747 1278 866
PciI A'CATG_T
Cuts at: 8419 8419
Size: 9520
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 4842 6064 1108
Size: 1602 2132 1222 4564
Fragments arranged by size:
4564 2132 1602 1222
PfoI T'CCnGG_A
Cuts at: 5206 5886 6078 5206
Size: 680 192 8648
Fragments arranged by size:
8648 680 192
PmeI GTTT'AAAC
Cuts at: 6824 6824
Size: 9520
PmlI CAC'GTG
Cuts at: 3674 3674
Size: 9520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2172 2204 7589 7621 9102 9134 2172
Size: 32 5385 32 1481 32 2558
Fragments arranged by size:
5385 2558 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2155 4862 5210 2155
Size: 2707 348 6465
Fragments arranged by size:
6465 2707 348
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9520
PsiI TTA'TAA
Cuts at: 7090 7090
Size: 9520
PstI C_TGCA'G
Cuts at: 830 2275 2596 2670 3015 5448 6872 830
Size: 1445 321 74 345 2433 1424 3478
Fragments arranged by size:
3478 2433 1445 1424 345 321 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9520
PvuII CAG'CTG
Cuts at: 5034 5305 6073 6796 5034
Size: 271 768 723 7758
Fragments arranged by size:
7758 768 723 271
SacI G_AGCT'C
Cuts at: 2394 4857 2394
Size: 2463 7057
SacII CC_GC'GG
Cuts at: 5405 5405
Size: 9520
SalI G'TCGA_C
Cuts at: 1936 6862 1936
Size: 4926 4594
SanDI GG'GwC_CC
Cuts at: 4862 5210 4862
Size: 348 9172
SapI GCTCTTCn'nnn_
Cuts at: 2577 2640 3190 5176 5681 2577
Size: 63 550 1986 505 6416
Fragments arranged by size:
6416 1986 550 505 63
SbfI CC_TGCA'GG
Cuts at: 6872 6872
Size: 9520
ScaI AGT'ACT
Cuts at: 7768 9480 7768
Size: 1712 7808
SexAI A'CCwGG_T
Cuts at: 3876 3876
Size: 9520
SfcI C'TryA_G
Cuts at: 826 1045 2271 2592 2666 3011 3943 3988 4528
Size: 219 1226 321 74 345 932 45 540
Cuts at: 4528 5444 6791 6868 8003 8684 8875 826
Size: 916 1347 77 1135 681 191 1471
Fragments arranged by size:
1471 1347 1226 1135 932 916 681 540
345 321 219 191 77 74 45
SfoI GGC'GCC
Cuts at: 6448 6448
Size: 9520
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9520
SmaI CCC'GGG
Cuts at: 1872 3322 5389 6847 1872
Size: 1450 2067 1458 4545
Fragments arranged by size:
4545 2067 1458 1450
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4041 4519 5939 7624 8525 8787
Size: 197 926 2098 478 1420 1685 901 262
Cuts at: 8787 820
Size: 1553
Fragments arranged by size:
2098 1685 1553 1420 926 901 478 262
197
SnaBI TAC'GTA
Cuts at: 493 6817 7311 493
Size: 6324 494 2702
Fragments arranged by size:
6324 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9520
SphI G_CATG'C
Cuts at: 1132 4331 6878 1132
Size: 3199 2547 3774
Fragments arranged by size:
3774 3199 2547
SspI AAT'ATT
Cuts at: 5 52 6165 7444 5
Size: 47 6113 1279 2081
Fragments arranged by size:
6113 2081 1279 47
StuI AGG'CCT
Cuts at: 1517 2312 4534 5582 1517
Size: 795 2222 1048 5455
Fragments arranged by size:
5455 2222 1048 795
StyI C'CwwG_G
Cuts at: 513 1065 2022 2127 2259 3514 3557 3788 4167
Size: 552 957 105 132 1255 43 231 379
Cuts at: 4167 4312 5457 6219 6510 6959 513
Size: 145 1145 762 291 449 3074
Fragments arranged by size:
3074 1255 1145 957 762 552 449 379
291 231 145 132 105 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 7547 7564 7717 7902 8347 325
Size: 1255 2661 3306 17 153 185 445 1498
Fragments arranged by size:
3306 2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 7766 9478 96
Size: 274 80 33 51 1443 5789 1712 138
Fragments arranged by size:
5789 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4117 4149 5073 5105 9480 9512 4117
Size: 32 924 32 4375 32 4125
Fragments arranged by size:
4375 4125 924 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3334 5777 5918 1316
Size: 2018 2443 141 4918
Fragments arranged by size:
4918 2443 2018 141
XbaI T'CTAG_A
Cuts at: 2422 4060 6856 2422
Size: 1638 2796 5086
Fragments arranged by size:
5086 2796 1638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2166 3415 6793 2166
Size: 1249 3378 4893
Fragments arranged by size:
4893 3378 1249
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9520
XmaI C'CCGG_G
Cuts at: 1870 3320 5387 6845 1870
Size: 1450 2067 1458 4545
Fragments arranged by size:
4545 2067 1458 1450
XmnI GAAnn'nnTTC
Cuts at: 6492 7649 6492
Size: 1157 8363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5704 7328 276
Size: 53 83 186 936 4170 1624 2468
Fragments arranged by size:
4170 2468 1624 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI
PsrI RsrII SfiI SrfI SwaI