Restriction map for
pFN21ASDF0258
(Circular) (Six-base) MAPSORT of: pFN21ASDF0258.seq Check: 5974 from: 1 to: 7033
pFN21ASDF0258 7033 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3260 4843 278
Size: 53 83 186 936 1724 1583 2468
Fragments arranged by size:
2468 1724 1583 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4376 1937
Size: 2439 4594
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2966 3188 3362 3445 3992 4106 5096 6480
Size: 1334 222 174 83 547 114 990 1384
Cuts at: 6480 6748 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1334 990 547 268 222 174
114 83
AfeI AGC'GCT
Cuts at: 4109 4109
Size: 7033
AflII C'TTAA_G
Cuts at: 820 1017 2546 820
Size: 197 1529 5307
Fragments arranged by size:
5307 1529 197
AflIII A'CryG_T
Cuts at: 1203 5932 6909 1203
Size: 4729 977 1327
Fragments arranged by size:
4729 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3676 1184
Size: 2492 4541
AlwNI CAG_nnn'CTG
Cuts at: 2041 2152 2767 3074 4123 4776 6348 2041
Size: 111 615 307 1049 653 1572 2726
Fragments arranged by size:
2726 1572 1049 653 615 307 111
ApaI G_GGCC'C
Cuts at: 2220 3444 3751 2220
Size: 1224 307 5502
Fragments arranged by size:
5502 1224 307
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 2246 4342 4568 4720 6829 1493
Size: 753 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 753 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 2212 3430 3533 3684 3731 4358 1870
Size: 73 269 1218 103 151 47 627 4545
Fragments arranged by size:
4545 1218 627 269 151 103 73 47
BamHI G'GATC_C
Cuts at: 3465 4363 4730 3465
Size: 898 367 5768
Fragments arranged by size:
5768 898 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3333 4354 5809 618
Size: 325 217 627 1546 1021 1455 1842
Fragments arranged by size:
1842 1546 1455 1021 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2220 2301 2336 2555 2627 3176 3444
Size: 673 156 81 35 219 72 549 268
Cuts at: 3444 3560 3684 3732 3751 4149 4259 4352 1391
Size: 116 124 48 19 398 110 93 4072
Fragments arranged by size:
4072 673 549 398 268 219 156 124
116 110 93 81 72 48 35 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3239 4328 928
Size: 971 1340 1089 3633
Fragments arranged by size:
3633 1340 1089 971
BbvCI CC'TCA_GC
Cuts at: 2099 2985 4244 2099
Size: 886 1259 4888
Fragments arranged by size:
4888 1259 886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3857 3891 5224 5258 1903
Size: 34 1920 34 1333 34 3678
Fragments arranged by size:
3678 1920 1333 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2812 4925 6135 2812
Size: 2113 1210 3710
Fragments arranged by size:
3710 2113 1210
BclI T'GATC_A
Cuts at: 1548 3130 1548
Size: 1582 5451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5644 136
Size: 107 122 71 631 605 3972 1525
Fragments arranged by size:
3972 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7028 7028
Size: 7033
BlpI GC'TnA_GC
Cuts at: 2075 4450 2075
Size: 2375 4658
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2844 3444 3751 5094 6250 1790
Size: 430 624 600 307 1343 1156 2573
Fragments arranged by size:
2573 1343 1156 624 600 430 307
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3005 4764 5722
Size: 416 53 83 399 24 1588 1759 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
1759 1753 1588 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2327 1056
Size: 1271 5762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 3574 2677
Size: 897 6136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3544 3814 3929 4100 5693 1239
Size: 824 1481 270 115 171 1593 2579
Fragments arranged by size:
2579 1593 1481 824 270 171 115
Bpu10I CC'TnA_GC
Cuts at: 2099 2319 2985 3678 4244 2099
Size: 220 666 693 566 4888
Fragments arranged by size:
4888 693 666 566 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5158 6023 6321 5158
Size: 865 298 5870
Fragments arranged by size:
5870 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2182 3116 3599 5696 882
Size: 617 683 934 483 2097 2219
Fragments arranged by size:
2219 2097 934 683 617 483
BsaAI yAC'GTr
Cuts at: 493 1204 2839 3595 4330 4824 493
Size: 711 1635 756 735 494 2702
Fragments arranged by size:
2702 1635 756 735 711 494
BsaBI GATnn'nnATC
Cuts at: 7027 7027
Size: 7033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3257 4840 5222 5857
Size: 53 83 186 936 1724 1583 382 635
Cuts at: 5857 275
Size: 1451
Fragments arranged by size:
1724 1583 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3571 5466 5864 6138 6285 6696 3571
Size: 1895 398 274 147 411 3908
Fragments arranged by size:
3908 1895 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2765 2795 2982
Size: 30 165 30 1032 30 300 30 187
Cuts at: 2982 3012 3755 3785 1178
Size: 30 743 30 4426
Fragments arranged by size:
4426 1032 743 300 187 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2621 2624 2669 2951 3284 3287 3740 3760
Size: 1435 3 45 282 333 3 453 20
Cuts at: 3760 3884 4076 4267 1186
Size: 124 192 191 3952
Fragments arranged by size:
3952 1435 453 333 282 192 191 124
45 20 3 3
BseYI C'CCAG_C
Cuts at: 2219 2300 3629 6236 6732 2219
Size: 81 1329 2607 496 2520
Fragments arranged by size:
2607 2520 1329 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3566 1163
Size: 573 1830 4630
Fragments arranged by size:
4630 1830 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2355 4483 5244 6272 1540
Size: 275 186 354 2128 761 1028 2301
Fragments arranged by size:
2301 2128 1028 761 354 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2555 5094 5179 6250 2064
Size: 491 2539 85 1071 2847
Fragments arranged by size:
2847 2539 1071 491 85
BsmI GAATG_Cn'
Cuts at: 2687 4544 4637 2687
Size: 1857 93 5083
Fragments arranged by size:
5083 1857 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3917 3917
Size: 7033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2220 2301 2336 2555 2627 2844
Size: 399 274 156 81 35 219 72 217
Cuts at: 2844 3176 3444 3560 3684 3732 3751 4149 4259
Size: 332 268 116 124 48 19 398 110
Cuts at: 4259 4352 5094 5179 6250 1391
Size: 93 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 332 274 268
219 217 156 124 116 110 93 85
81 72 48 35 19
BspEI T'CCGG_A
Cuts at: 3571 6696 3571
Size: 3125 3908
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 2831 3258 3390 3796 4388 844
Size: 1523 464 427 132 406 592 3489
Fragments arranged by size:
3489 1523 592 464 427 406 132
BsrBI CCG'CTC
Cuts at: 1394 1823 2371 4920 1394
Size: 429 548 2549 3507
Fragments arranged by size:
3507 2549 548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2580 5528 5702 65
Size: 1183 1332 2948 174 1396
Fragments arranged by size:
2948 1396 1332 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2853 3477 5677 5864 1700
Size: 110 1043 624 2200 187 2869
Fragments arranged by size:
2869 2200 1043 624 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 2090 2867 5093 6105 2090
Size: 777 2226 1012 3018
Fragments arranged by size:
3018 2226 1012 777
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2152 2872 794
Size: 1358 720 4955
Fragments arranged by size:
4955 1358 720
BstBI TT'CG_AA
Cuts at: 2007 5837 2007
Size: 3830 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2671 2227
Size: 444 6589
BstYI r'GATC_y
Cuts at: 1972 3005 3465 3656 4363 4730 5115 5132 6584
Size: 1033 460 191 707 367 385 17 1452
Cuts at: 6584 6670 6682 7028 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1033 707 460 385 367 346
191 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2391 2391
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 2022 2130 4026 513
Size: 552 957 108 1896 3520
Fragments arranged by size:
3520 1896 957 552 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3388 487
Size: 683 56 2162 4132
Fragments arranged by size:
4132 2162 683 56
BtsI GCAGTG_nn'
Cuts at: 2732 2912 3784 4549 5343 5363 2732
Size: 180 872 765 794 20 4402
Fragments arranged by size:
4402 872 794 765 180 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DraIII CAC_nnn'GTG
Cuts at: 2674 2674
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3806 6040 809
Size: 507 2490 2234 1802
Fragments arranged by size:
2490 2234 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2352 3112
Size: 54 1425 50 275 18 297 225 760
Cuts at: 3112 3240 3489 4029 4480 5369 6998 8
Size: 128 249 540 451 889 1629 43
Fragments arranged by size:
1629 1425 889 760 540 451 297 275
249 225 128 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2352 4480 1537
Size: 275 540 2128 4090
Fragments arranged by size:
4090 2128 540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2598 2615 3143 3956 4244 4963 1353
Size: 1047 198 17 528 813 288 719 3423
Fragments arranged by size:
3423 1047 813 719 528 288 198 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2062 2553 2062
Size: 491 6542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2966 3188 3362 3445 3544 3814
Size: 393 431 903 222 174 83 99 270
Cuts at: 3814 3929 3992 4100 4106 5096 5693 6480 6748
Size: 115 63 108 6 990 597 787 268
Cuts at: 6748 1239
Size: 1524
Fragments arranged by size:
1524 990 903 787 597 431 393 270
268 222 174 115 108 99 83 63
6
EcoO109I rG'GnC_Cy
Cuts at: 2216 2410 2423 2998 3441 3747 2216
Size: 194 13 575 443 306 5502
Fragments arranged by size:
5502 575 443 306 194 13
EcoRI G'AATT_C
Cuts at: 4342 4720 4342
Size: 378 6655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2429 4126 2429
Size: 1697 5336
FspI TGC'GCA
Cuts at: 3473 5539 3473
Size: 2066 4967
HaeII r_GCGC'y
Cuts at: 1678 3581 3739 4069 4111 6180 1678
Size: 1903 158 330 42 2069 2531
Fragments arranged by size:
2531 2069 1903 330 158 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2982 2997 3014
Size: 32 135 32 623 32 755 15 17
Cuts at: 3014 3029 3115 3147 5674 5706 5748 5780 1373
Size: 15 86 32 2527 32 42 32 2626
Fragments arranged by size:
2626 2527 755 623 135 86 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 4228 4377 4623 669
Size: 988 281 2290 149 246 3079
Fragments arranged by size:
3079 2290 988 281 246 149
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3009 4299 6147 6331 1305
Size: 1704 1290 1848 184 2007
Fragments arranged by size:
2007 1848 1704 1290 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3491 4031 10
Size: 54 1425 343 1659 540 3012
Fragments arranged by size:
3012 1659 1425 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2837 4991 5350 5509 518
Size: 648 1671 2154 359 159 2042
Fragments arranged by size:
2154 2042 1671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2740 4449 5126 6274 6519 1606
Size: 444 690 1709 677 1148 245 2120
Fragments arranged by size:
2120 1709 1148 690 677 444 245
NaeI GCC'GGC
Cuts at: 2855 2855
Size: 7033
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5524
Fragments arranged by size:
5524 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NgoMIV G'CCGG_C
Cuts at: 2853 2853
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 2323 1052
Size: 1271 5762
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 2281 4391 5936 1132
Size: 97 1052 2110 1545 2229
Fragments arranged by size:
2229 2110 1545 1052 97
PasI CC'CwG_GG
Cuts at: 1348 2884 4141 1348
Size: 1536 1257 4240
Fragments arranged by size:
4240 1536 1257
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2041 3796 4308 1108
Size: 933 1755 512 3833
Fragments arranged by size:
3833 1755 933 512
PfoI T'CCnGG_A
Cuts at: 2082 2082
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2423 2998 2423
Size: 575 6458
PshAI GACnn'nnGTC
Cuts at: 1613 3522 1613
Size: 1909 5124
PsiI TTA'TAA
Cuts at: 4603 4603
Size: 7033
PspOMI G'GGCC_C
Cuts at: 2216 3440 3747 2216
Size: 1224 307 5502
Fragments arranged by size:
5502 1224 307
PstI C_TGCA'G
Cuts at: 830 2148 2157 2468 2828 2919 3255 3779 4385
Size: 1318 9 311 360 91 336 524 606
Cuts at: 4385 830
Size: 3478
Fragments arranged by size:
3478 1318 606 524 360 336 311 91
9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 2050 2740 2050
Size: 690 6343
SacI G_AGCT'C
Cuts at: 2064 2555 2064
Size: 491 6542
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SanDI GG'GwC_CC
Cuts at: 2423 2423
Size: 7033
SapI GCTCTTCn'nnn_
Cuts at: 2615 3143 2615
Size: 528 6505
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SexAI A'CCwGG_T
Cuts at: 2670 2670
Size: 7033
SfcI C'TryA_G
Cuts at: 826 1045 2144 2153 2464 2824 2915 3251 3775
Size: 219 1099 9 311 360 91 336 524
Cuts at: 3775 4292 4381 5516 6197 6388 826
Size: 517 89 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 681 524 517 360 336
311 219 191 91 89 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 3432 4360 1872
Size: 1560 928 4545
Fragments arranged by size:
4545 1560 928
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 5137 6038 6300 820
Size: 197 926 603 2591 901 262 1553
Fragments arranged by size:
2591 1553 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 4330 4824 493
Size: 3837 494 2702
Fragments arranged by size:
3837 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 4957 5
Size: 47 4905 2081
Fragments arranged by size:
4905 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 5890
StyI C'CwwG_G
Cuts at: 513 1065 2022 2784 2844 3109 4279 4302 4472
Size: 552 957 762 60 265 1170 23 170
Cuts at: 4472 513
Size: 3074
Fragments arranged by size:
3074 1170 957 762 552 265 170 60
23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2043 2897 3074 3080 3465 5060 5077
Size: 1255 463 854 177 6 385 1595 17
Cuts at: 5077 5230 5415 5860 325
Size: 153 185 445 1498
Fragments arranged by size:
1595 1498 1255 854 463 445 385 185
177 153 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 5279 6991
Size: 274 80 33 51 1443 465 2837 1712
Cuts at: 6991 96
Size: 138
Fragments arranged by size:
2837 1712 1443 465 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3317 3349 3755 3787 4031 4063 6993 7025 3317
Size: 32 406 32 244 32 2930 32 3325
Fragments arranged by size:
3325 2930 406 244 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 3430 4358 1870
Size: 1560 928 4545
Fragments arranged by size:
4545 1560 928
XmnI GAAnn'nnTTC
Cuts at: 3981 5162 3981
Size: 1181 5852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3258 4841 276
Size: 53 83 186 936 1724 1583 2468
Fragments arranged by size:
2468 1724 1583 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI XcmI