Restriction map for
pFN21ASDF0201
(Circular) (Six-base) MAPSORT of: pFN21ASDF0201.seq Check: 5549 from: 1 to: 6838
pFN21ASDF0201 6838 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3666 3869 3666
Size: 203 6635
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2853 3879 4181 1937
Size: 916 1026 302 4594
Fragments arranged by size:
4594 1026 916 302
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2267 3488 4901 6285 6553 1632
Size: 635 1221 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 1221 635 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 3918 5737 6714 1203
Size: 2715 1819 977 1327
Fragments arranged by size:
2715 1819 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 5567 5567
Size: 6838
AleI CACnn'nnGTG
Cuts at: 1166 3758 3917 1166
Size: 2592 159 4087
Fragments arranged by size:
4087 2592 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2706 1184
Size: 1522 5316
AlwNI CAG_nnn'CTG
Cuts at: 2212 2825 3170 3848 4581 6153 2212
Size: 613 345 678 733 1572 2897
Fragments arranged by size:
2897 1572 733 678 613 345
ApaI G_GGCC'C
Cuts at: 2370 2519 2571 2598 2616 2677 2764 2834 2903
Size: 149 52 27 18 61 87 70 69
Cuts at: 2903 2939 3008 3080 3144 3271 3297 3450 3495
Size: 36 69 72 64 127 26 153 45
Cuts at: 3495 3537 3546 3581 3600 2370
Size: 42 9 35 19 5608
Fragments arranged by size:
5608 153 149 127 87 72 70 69
69 64 61 52 45 42 36 35
27 26 19 18 9
ApaLI G'TGCA_C
Cuts at: 3899 4895 6051 3899
Size: 996 1156 4686
Fragments arranged by size:
4686 1156 996
ApoI r'AATT_y
Cuts at: 1493 3800 4147 4373 4525 6634 1493
Size: 2307 347 226 152 2109 1697
Fragments arranged by size:
2307 2109 1697 347 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2276 2283 2512 2660 2931 3593 3601
Size: 73 333 7 229 148 271 662 8
Cuts at: 3601 3784 4163 1870
Size: 183 379 4545
Fragments arranged by size:
4545 662 379 333 271 229 183 148
73 8 7
BamHI G'GATC_C
Cuts at: 4168 4535 4168
Size: 367 6471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2549 2560 2690 3614 3680
Size: 325 217 627 762 11 130 924 66
Cuts at: 3680 3911 4159 5614 618
Size: 231 248 1455 1842
Fragments arranged by size:
1842 1455 924 762 627 325 248 231
217 130 66 11
BanII G_rGCy'C
Cuts at: 1391 2259 2370 2412 2519 2571 2598 2616 2677
Size: 868 111 42 107 52 27 18 61
Cuts at: 2677 2764 2834 2903 2939 3008 3080 3144 3271
Size: 87 70 69 36 69 72 64 127
Cuts at: 3271 3297 3352 3378 3450 3495 3537 3546 3581
Size: 26 55 26 72 45 42 9 35
Cuts at: 3581 3600 4157 1391
Size: 19 557 4072
Fragments arranged by size:
4072 868 557 127 111 107 87 72
72 70 69 69 64 61 55 52
45 42 42 36 35 27 26 26
19 18 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2040 928
Size: 971 141 5726
Fragments arranged by size:
5726 971 141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3713 3747 5029 5063 1903
Size: 34 1776 34 1282 34 3678
Fragments arranged by size:
3678 1776 1282 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4730 5940 4730
Size: 1210 5628
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2123 2304 2434
Size: 107 122 71 631 605 451 181 130
Cuts at: 2434 2514 2523 2706 2961 3148 3462 3541 3595
Size: 80 9 183 255 187 314 79 54
Cuts at: 3595 3623 3746 3831 3870 5449 136
Size: 28 123 85 39 1579 1525
Fragments arranged by size:
1579 1525 631 605 451 314 255 187
183 181 130 123 122 107 85 80
79 71 54 39 28 9
BglII A'GATC_T
Cuts at: 6833 6833
Size: 6838
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2370 2519 2554 2571 2598 2616 2677 2695
Size: 580 149 35 17 27 18 61 18
Cuts at: 2695 2764 2834 2903 2939 3008 3080 3144 3271
Size: 69 70 69 36 69 72 64 127
Cuts at: 3271 3297 3450 3495 3537 3546 3581 3600 3619
Size: 26 153 45 42 9 35 19 19
Cuts at: 3619 3903 3914 4899 6055 1790
Size: 284 11 985 1156 2573
Fragments arranged by size:
2573 1156 985 580 284 153 149 127
72 70 69 69 69 64 61 45
42 36 35 35 27 26 19 19
18 18 17 11 9
BmgBI CAC'GTC
Cuts at: 1318 3137 3904 1318
Size: 1819 767 4252
Fragments arranged by size:
4252 1819 767
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2135 3300 4569
Size: 416 53 83 399 24 718 1165 1269
Cuts at: 4569 5527 442
Size: 958 1753
Fragments arranged by size:
1753 1269 1165 958 718 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2814 2868 3192 3348 3407 3519 4001 5498
Size: 1575 54 324 156 59 112 482 1497
Cuts at: 5498 1239
Size: 2579
Fragments arranged by size:
2579 1575 1497 482 324 156 112 59
54
Bpu10I CC'TnA_GC
Cuts at: 2728 2728
Size: 6838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 4963 5828 6126 2618
Size: 2345 865 298 3330
Fragments arranged by size:
3330 2345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5501 882
Size: 617 4002 2219
Fragments arranged by size:
4002 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4135 4629 493
Size: 711 2931 494 2702
Fragments arranged by size:
2931 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 3876 4645 5027
Size: 53 83 186 936 566 1777 769 382
Cuts at: 5027 5662 275
Size: 635 1451
Fragments arranged by size:
1777 1451 936 769 635 566 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3807 5271 5669 5943 6090 6501 3807
Size: 1464 398 274 147 411 4144
Fragments arranged by size:
4144 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3565 3595 4082 4112 1178
Size: 30 165 30 2162 30 487 30 3904
Fragments arranged by size:
3904 2162 487 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4090 1186
Size: 2904 3934
BseYI C'CCAG_C
Cuts at: 2026 2080 2363 3007 3011 3227 3530 3688 3718
Size: 54 283 644 4 216 303 158 30
Cuts at: 3718 3841 6041 6537 2026
Size: 123 2200 496 2327
Fragments arranged by size:
2327 2200 644 496 303 283 216 158
123 54 30 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4067 1163
Size: 573 2331 3934
Fragments arranged by size:
3934 2331 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3765 3903 4017 4899 4984 6055 3765
Size: 138 114 882 85 1071 4548
Fragments arranged by size:
4548 1071 882 138 114 85
BsmI GAATG_Cn'
Cuts at: 3195 4349 4442 3195
Size: 1154 93 5591
Fragments arranged by size:
5591 1154 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2370 2412 2519 2554 2571 2598
Size: 399 469 111 42 107 35 17 27
Cuts at: 2598 2616 2677 2695 2764 2834 2903 2939 3008
Size: 18 61 18 69 70 69 36 69
Cuts at: 3008 3080 3144 3271 3297 3352 3378 3450 3495
Size: 72 64 127 26 55 26 72 45
Cuts at: 3495 3537 3546 3581 3600 3619 3765 3903 3914
Size: 42 9 35 19 19 146 138 11
Cuts at: 3914 4017 4157 4899 4984 6055 1391
Size: 103 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 146 140 138
127 111 107 103 85 72 72 70
69 69 69 64 61 55 45 42
42 36 35 35 27 26 26 19
19 18 18 17 11 9
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3666 3869 4193 844
Size: 2822 203 324 3489
Fragments arranged by size:
3489 2822 324 203
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5333 5507 65
Size: 1183 4085 174 1396
Fragments arranged by size:
4085 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2117 3745 3949 5482 5669 1700
Size: 110 307 1628 204 1533 187 2869
Fragments arranged by size:
2869 1628 1533 307 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 3933 96
Size: 3837 3001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6838
BstBI TT'CG_AA
Cuts at: 2007 5642 2007
Size: 3635 3203
BstEII G'GTnAC_C
Cuts at: 1184 3203 1184
Size: 2019 4819
BstXI CCAn_nnnn'nTGG
Cuts at: 4001 4001
Size: 6838
BstYI r'GATC_y
Cuts at: 1972 4168 4535 4920 4937 6389 6475 6487 6833
Size: 2196 367 385 17 1452 86 12 346
Cuts at: 6833 1972
Size: 1977
Fragments arranged by size:
2196 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2521 3153 3395 3406 2521
Size: 632 242 11 5953
Fragments arranged by size:
5953 632 242 11
BtgI C'CryG_G
Cuts at: 513 1065 2093 2344 3271 3537 4083 513
Size: 552 1028 251 927 266 546 3268
Fragments arranged by size:
3268 1028 927 552 546 266 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3628 487
Size: 683 56 2402 3697
Fragments arranged by size:
3697 2402 683 56
BtsI GCAGTG_nn'
Cuts at: 3753 4354 5148 5168 3753
Size: 601 794 20 5423
Fragments arranged by size:
5423 794 601 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2044 3426 5845 809
Size: 507 728 1382 2419 1802
Fragments arranged by size:
2419 1802 1382 728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3823 3951 4285
Size: 54 1425 50 275 18 1993 128 334
Cuts at: 4285 5174 6803 8
Size: 889 1629 43
Fragments arranged by size:
1993 1629 1425 889 334 275 128 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4768 1353
Size: 3415 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5425 5799 5945 387
Size: 1064 138 3836 374 146 1280
Fragments arranged by size:
3836 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2267 2814 2868 3192 3348 3407 3488
Size: 393 635 547 54 324 156 59 81
Cuts at: 3488 3519 4001 4901 5498 6285 6553 1239
Size: 31 482 900 597 787 268 1524
Fragments arranged by size:
1524 900 787 635 597 547 482 393
324 268 156 81 59 54 31
EcoNI CCTnn'n_nnAGG
Cuts at: 3992 3992
Size: 6838
EcoO109I rG'GnC_Cy
Cuts at: 2171 2288 2367 2479 2489 2498 2516 2567 2568
Size: 117 79 112 10 9 18 51 1
Cuts at: 2568 2594 2595 2613 2673 2674 2760 2761 2804
Size: 26 1 18 60 1 86 1 43
Cuts at: 2804 2840 2899 2928 2935 2936 2954 2987 3004
Size: 36 59 29 7 1 18 33 17
Cuts at: 3004 3016 3076 3095 3141 3167 3176 3212 3241
Size: 12 60 19 46 26 9 36 29
Cuts at: 3241 3267 3401 3447 3491 3492 3542 3543 3553
Size: 26 134 46 44 1 50 1 10
Cuts at: 3553 3578 3587 3597 3605 3624 2171
Size: 25 9 10 8 19 5385
Fragments arranged by size:
5385 134 117 112 86 79 60 60
59 51 50 46 46 44 43 36
36 33 29 29 26 26 26 25
19 19 18 18 18 17 12 10
10 10 9 9 9 8 7 1
1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 4147 4525 4147
Size: 378 6460
FspI TGC'GCA
Cuts at: 5344 5344
Size: 6838
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3983 4015 4104 4136 5479
Size: 32 135 32 2411 32 89 32 1343
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2411 1343 135 89 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4038 5952 6136 1305
Size: 2733 1914 184 2007
Fragments arranged by size:
2733 2007 1914 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3825 10
Size: 54 1425 343 1993 3023
Fragments arranged by size:
3023 1993 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3758 3794 3917 4796 5155 5314 518
Size: 648 2592 36 123 879 359 159 2042
Fragments arranged by size:
2592 2042 879 648 359 159 123 36
MspA1I CmG'CkG
Cuts at: 1606 2095 2230 2723 3011 3434 3831 4021 4254
Size: 489 135 493 288 423 397 190 233
Cuts at: 4254 4931 6079 6324 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 493 489 423 397 288
245 233 190 135
NaeI GCC'GGC
Cuts at: 3951 3951
Size: 6838
NcoI C'CATG_G
Cuts at: 513 1065 2344 3271 513
Size: 552 1279 927 4080
Fragments arranged by size:
4080 1279 927 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6838
NgoMIV G'CCGG_C
Cuts at: 3949 3949
Size: 6838
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 3793 4196 5741 1132
Size: 97 2564 403 1545 2229
Fragments arranged by size:
2564 2229 1545 403 97
PasI CC'CwG_GG
Cuts at: 1348 2267 2405 2599 2678 2808 2870 2942 3146
Size: 919 138 194 79 130 62 72 204
Cuts at: 3146 3352 3380 3548 3620 1348
Size: 206 28 168 72 4566
Fragments arranged by size:
4566 919 206 204 194 168 138 130
79 72 72 62 28
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2212 3713 3848 3921 1108
Size: 1104 1501 135 73 4025
Fragments arranged by size:
4025 1501 1104 135 73
PfoI T'CCnGG_A
Cuts at: 2381 2791 2845 2381
Size: 410 54 6374
Fragments arranged by size:
6374 410 54
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2171 2288 2489 2498 2840 2928 3016 3095 3176
Size: 117 201 9 342 88 88 79 81
Cuts at: 3176 3241 3401 3553 3605 2171
Size: 65 160 152 52 5404
Fragments arranged by size:
5404 342 201 160 152 117 88 88
81 79 65 52 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspOMI G'GGCC_C
Cuts at: 2366 2515 2567 2594 2612 2673 2760 2830 2899
Size: 149 52 27 18 61 87 70 69
Cuts at: 2899 2935 3004 3076 3140 3267 3293 3446 3491
Size: 36 69 72 64 127 26 153 45
Cuts at: 3491 3533 3542 3577 3596 2366
Size: 42 9 35 19 5608
Fragments arranged by size:
5608 153 149 127 87 72 70 69
69 64 61 52 45 42 36 35
27 26 19 18 9
PstI C_TGCA'G
Cuts at: 830 3121 4190 830
Size: 2291 1069 3478
Fragments arranged by size:
3478 2291 1069
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2723 3011 3434 4021 2723
Size: 288 423 587 5540
Fragments arranged by size:
5540 587 423 288
SacII CC_GC'GG
Cuts at: 2096 2096
Size: 6838
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SanDI GG'GwC_CC
Cuts at: 2288 2489 2928 3016 3095 3241 3401 3553 3605
Size: 201 439 88 79 146 160 152 52
Cuts at: 3605 2288
Size: 5521
Fragments arranged by size:
5521 439 201 160 152 146 88 79
52
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SexAI A'CCwGG_T
Cuts at: 2545 2737 4035 2545
Size: 192 1298 5348
Fragments arranged by size:
5348 1298 192
SfcI C'TryA_G
Cuts at: 826 1045 3117 4186 5321 6002 6193 826
Size: 219 2072 1069 1135 681 191 1471
Fragments arranged by size:
2072 1471 1135 1069 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2514 2706 3148 3462 3541 3595 2514
Size: 192 442 314 79 54 5757
Fragments arranged by size:
5757 442 314 192 79 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 2278 2514 2662 2933 3595 3603 4165 1872
Size: 406 236 148 271 662 8 562 4545
Fragments arranged by size:
4545 662 562 406 271 236 148 8
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 4942 5843 6105 820
Size: 197 926 690 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 690 262 197
SnaBI TAC'GTA
Cuts at: 493 4135 4629 493
Size: 3642 494 2702
Fragments arranged by size:
3642 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 3793 4196 1132
Size: 2661 403 3774
Fragments arranged by size:
3774 2661 403
SrfI GCCC'GGGC
Cuts at: 2278 2662 2278
Size: 384 6454
SspI AAT'ATT
Cuts at: 5 52 4762 5
Size: 47 4710 2081
Fragments arranged by size:
4710 2081 47
StuI AGG'CCT
Cuts at: 1517 2631 3456 3483 1517
Size: 1114 825 27 4872
Fragments arranged by size:
4872 1114 825 27
StyI C'CwwG_G
Cuts at: 513 1065 2344 2554 2991 3271 3297 3451 4277
Size: 552 1279 210 437 280 26 154 826
Cuts at: 4277 513
Size: 3074
Fragments arranged by size:
3074 1279 826 552 437 280 210 154
26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3913 4033 4865 4882 5035 5220 5665
Size: 1255 2333 120 832 17 153 185 445
Cuts at: 5665 325
Size: 1498
Fragments arranged by size:
2333 1498 1255 832 445 185 153 120
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 3933 3972
Size: 274 80 33 51 1443 171 1785 39
Cuts at: 3972 4089 5084 6796 96
Size: 117 995 1712 138
Fragments arranged by size:
1785 1712 1443 995 274 171 138 117
80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3324 3356 3565 3597 6798 6830 3324
Size: 32 209 32 3201 32 3332
Fragments arranged by size:
3332 3201 209 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4037 1316
Size: 2721 4117
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2351 2606 3215 3278 3431 3670 3922 4001 2351
Size: 255 609 63 153 239 252 79 5188
Fragments arranged by size:
5188 609 255 252 239 153 79 63
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 2276 2512 2660 2931 3593 3601 4163 1870
Size: 406 236 148 271 662 8 562 4545
Fragments arranged by size:
4545 662 562 406 271 236 148 8
XmnI GAAnn'nnTTC
Cuts at: 4967 4967
Size: 6838
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SapI SfoI SwaI