Restriction map for
pFN21ASDF0166
(Circular) (Six-base) MAPSORT of: pFN21ASDF0166.seq Check: 6256 from: 1 to: 6046
pFN21ASDF0166 6046 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 3301 2284
Size: 1017 5029
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3389 1937
Size: 1452 4594
Acc65I G'GTAC_C
Cuts at: 3367 3367
Size: 6046
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2721 2913 4109 5493 5761 1632
Size: 1089 192 1196 1384 268 1917
Fragments arranged by size:
1917 1384 1196 1089 268 192
AflII C'TTAA_G
Cuts at: 820 1017 2521 820
Size: 197 1504 4345
Fragments arranged by size:
4345 1504 197
AflIII A'CryG_T
Cuts at: 1203 4945 5922 1203
Size: 3742 977 1327
Fragments arranged by size:
3742 1327 977
AgeI A'CCGG_T
Cuts at: 4877 4877
Size: 6046
AhdI GACnn_n'nnGTC
Cuts at: 4775 4775
Size: 6046
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 3789 5361 3789
Size: 1572 4474
ApaI G_GGCC'C
Cuts at: 2156 2156
Size: 6046
ApaLI G'TGCA_C
Cuts at: 4103 5259 4103
Size: 1156 4890
ApoI r'AATT_y
Cuts at: 1493 2219 2435 2813 2884 3336 3355 3581 3733
Size: 726 216 378 71 452 19 226 152
Cuts at: 3733 5842 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 726 452 378 226 216 152
71 19
AseI AT'TA_AT
Cuts at: 160 1032 2292 4600 160
Size: 872 1260 2308 1606
Fragments arranged by size:
2308 1606 1260 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2148 3371 1870
Size: 73 205 1223 4545
Fragments arranged by size:
4545 1223 205 73
BamHI G'GATC_C
Cuts at: 2554 3376 3743 2554
Size: 822 367 4857
Fragments arranged by size:
4857 822 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3367 4822 618
Size: 325 217 627 1580 1455 1842
Fragments arranged by size:
1842 1580 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2156 3365 1391
Size: 765 1209 4072
Fragments arranged by size:
4072 1209 765
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2304 928
Size: 971 405 4670
Fragments arranged by size:
4670 971 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3938 5148 3938
Size: 1210 4836
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2156 4107 5263 1790
Size: 366 1951 1156 2573
Fragments arranged by size:
2573 1951 1156 366
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3777 4735 442
Size: 416 53 83 399 24 2360 958 1753
Fragments arranged by size:
2360 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2633 1056
Size: 1577 4469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4706 1239
Size: 3467 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4709 882
Size: 617 3210 2219
Fragments arranged by size:
3210 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3343 3837 493
Size: 711 2139 494 2702
Fragments arranged by size:
2702 2139 711 494
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2986 3853 4235 4870
Size: 53 83 186 936 1453 867 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1453 1451 936 867 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4479 4877 5151 5298 5709 4479
Size: 398 274 147 411 4816
Fragments arranged by size:
4816 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3201 1186
Size: 2015 4031
BseYI C'CCAG_C
Cuts at: 2163 5249 5745 2163
Size: 3086 496 2464
Fragments arranged by size:
3086 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 3496 4257 5285 1540
Size: 275 186 135 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186 135
BsiHKAI G_wGCw'C
Cuts at: 4107 4192 5263 4107
Size: 85 1071 4890
Fragments arranged by size:
4890 1071 85
BsmI GAATG_Cn'
Cuts at: 3557 3650 3557
Size: 93 5953
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 3365 4107 4192 5263 1391
Size: 399 366 1209 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 399 366 85
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 2478 3935 5665 2478
Size: 1457 1730 2859
Fragments arranged by size:
2859 1730 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 3301 3401 844
Size: 1440 1017 100 3489
Fragments arranged by size:
3489 1440 1017 100
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4541 4715 65
Size: 1183 3293 174 1396
Fragments arranged by size:
3293 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4690 4877 1700
Size: 110 2880 187 2869
Fragments arranged by size:
2880 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 3060 4106 5118 3060
Size: 1046 1012 3988
Fragments arranged by size:
3988 1046 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2173 3074 3281 794
Size: 1379 901 207 3559
Fragments arranged by size:
3559 1379 901 207
BstBI TT'CG_AA
Cuts at: 2007 4850 2007
Size: 2843 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 2239 2554 3376 3743 4128 4145 5597 5683
Size: 267 315 822 367 385 17 1452 86
Cuts at: 5683 5695 6041 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 822 385 367 346 315 267
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2059 2548 513
Size: 552 957 37 489 4011
Fragments arranged by size:
4011 957 552 489 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 2946 2977 3219 3268 3350 2946
Size: 31 242 49 82 5642
Fragments arranged by size:
5642 242 82 49 31
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3493 4382
Size: 54 1425 50 275 18 303 1360 889
Cuts at: 4382 6011 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1360 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2133 3493 1537
Size: 275 321 1360 4090
Fragments arranged by size:
4090 1360 321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 2656 2661 2697 3976 1353
Size: 833 470 5 36 1279 3423
Fragments arranged by size:
3423 1279 833 470 36 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4633 5007 5153 387
Size: 1064 138 3044 374 146 1280
Fragments arranged by size:
3044 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2721 2913 4109 4706 5493 5761 1239
Size: 393 1089 192 1196 597 787 268 1524
Fragments arranged by size:
1524 1196 1089 787 597 393 268 192
EcoO109I rG'GnC_Cy
Cuts at: 2032 2152 2032
Size: 120 5926
EcoRI G'AATT_C
Cuts at: 2435 2813 3355 3733 2435
Size: 378 542 378 4748
Fragments arranged by size:
4748 542 378 378
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2583 2615 4687
Size: 32 135 32 974 32 5 32 2072
Cuts at: 4687 4719 4761 4793 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2072 974 135 42 32 32 32
32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2200 2968 3390 3636 669
Size: 988 281 262 768 422 246 3079
Fragments arranged by size:
3079 988 768 422 281 262 246
HindIII A'AGCT_T
Cuts at: 748 3018 3048 3243 748
Size: 2270 30 195 3551
Fragments arranged by size:
3551 2270 195 30
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 3371 3371
Size: 6046
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2962 3161 5160 5344 1305
Size: 1657 199 1999 184 2007
Fragments arranged by size:
2007 1999 1657 199 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4224
Fragments arranged by size:
4224 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2994 3147 4004 4363 4522 518
Size: 648 1828 153 857 359 159 2042
Fragments arranged by size:
2042 1828 857 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 3462 4139 5287 5532 1606
Size: 1856 677 1148 245 2120
Fragments arranged by size:
2120 1856 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2022 2548 513
Size: 552 957 526 4011
Fragments arranged by size:
4011 957 552 526
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 2629 1052
Size: 1577 4469
NotI GC'GGCC_GC
Cuts at: 3493 3493
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3404 4949 1132
Size: 97 2175 1545 2229
Fragments arranged by size:
2229 2175 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6046
PciI A'CATG_T
Cuts at: 4945 4945
Size: 6046
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PfoI T'CCnGG_A
Cuts at: 2258 2800 2258
Size: 542 5504
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2032 2032
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3616 3616
Size: 6046
PspOMI G'GGCC_C
Cuts at: 2152 2152
Size: 6046
PstI C_TGCA'G
Cuts at: 830 3398 830
Size: 2568 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 3388 1936
Size: 1452 4594
SapI GCTCTTCn'nnn_
Cuts at: 2656 2697 2656
Size: 41 6005
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2719 3394 4529 5210 5401 826
Size: 219 1674 675 1135 681 191 1471
Fragments arranged by size:
1674 1471 1135 681 675 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 2150 3373 1872
Size: 278 1223 4545
Fragments arranged by size:
4545 1223 278
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2521 4150 5051 5313 820
Size: 197 926 578 1629 901 262 1553
Fragments arranged by size:
1629 1553 926 901 578 262 197
SnaBI TAC'GTA
Cuts at: 493 3343 3837 493
Size: 2850 494 2702
Fragments arranged by size:
2850 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 2871 1517
Size: 1354 4692
StyI C'CwwG_G
Cuts at: 513 1065 2022 2548 2784 3485 513
Size: 552 957 526 236 701 3074
Fragments arranged by size:
3074 957 701 552 526 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4073 4090 4243 4428 4873 325
Size: 1255 2493 17 153 185 445 1498
Fragments arranged by size:
2493 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4292 6004 96
Size: 274 80 33 51 1443 2315 1712 138
Fragments arranged by size:
2315 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2976 3008 6006 6038 2976
Size: 32 2998 32 2984
Fragments arranged by size:
2998 2984 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6046
XbaI T'CTAG_A
Cuts at: 2821 3382 2821
Size: 561 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 2148 3371 1870
Size: 278 1223 4545
Fragments arranged by size:
4545 1223 278
XmnI GAAnn'nnTTC
Cuts at: 2422 2706 2817 4175 2422
Size: 284 111 1358 4293
Fragments arranged by size:
4293 1358 284 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI