Restriction map for
pFN21ASDF0128
(Circular) (Six-base) MAPSORT of: pFN21ASDF0128.seq Check: 1085 from: 1 to: 9286
pFN21ASDF0128 9286 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2202 2335 4384 2202
Size: 133 2049 7104
Fragments arranged by size:
7104 2049 133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6393 7096 278
Size: 53 83 186 936 4857 703 2468
Fragments arranged by size:
4857 2468 936 703 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5166 6629 1937
Size: 3229 1463 4594
Fragments arranged by size:
4594 3229 1463
Acc65I G'GTAC_C
Cuts at: 2179 2690 2833 3131 6607 2179
Size: 511 143 298 3476 4858
Fragments arranged by size:
4858 3476 511 298 143
AclI AA'CG_TT
Cuts at: 1563 7413 7786 1563
Size: 5850 373 3063
Fragments arranged by size:
5850 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2820 3138 3420 3839 5441 5840 5961 7349
Size: 1188 318 282 419 1602 399 121 1388
Cuts at: 7349 8733 9001 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1602 1388 1384 1188 419 399 318
282 268 121
AfeI AGC'GCT
Cuts at: 4507 4649 4507
Size: 142 9144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9089
AflIII A'CryG_T
Cuts at: 1203 5191 8185 9162 1203
Size: 3988 2994 977 1327
Fragments arranged by size:
3988 2994 1327 977
AgeI A'CCGG_T
Cuts at: 8117 8117
Size: 9286
AhdI GACnn_n'nnGTC
Cuts at: 4462 8015 4462
Size: 3553 5733
AleI CACnn'nnGTG
Cuts at: 1166 2386 2464 5159 1166
Size: 1220 78 2695 5293
Fragments arranged by size:
5293 2695 1220 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2214 5795 1184
Size: 1030 3581 4675
Fragments arranged by size:
4675 3581 1030
AlwNI CAG_nnn'CTG
Cuts at: 2514 2548 2798 2987 3041 3350 3778 3889 4038
Size: 34 250 189 54 309 428 111 149
Cuts at: 4038 4378 4603 4706 5287 5644 6133 7029 8601
Size: 340 225 103 581 357 489 896 1572
Cuts at: 8601 2514
Size: 3199
Fragments arranged by size:
3199 1572 896 581 489 428 357 340
309 250 225 189 149 111 103 54
34
ApaI G_GGCC'C
Cuts at: 4418 4636 4756 4795 4957 4968 5084 5728 5939
Size: 218 120 39 162 11 116 644 211
Cuts at: 5939 4418
Size: 7765
Fragments arranged by size:
7765 644 218 211 162 120 116 39
11
ApaLI G'TGCA_C
Cuts at: 2582 6057 7343 8499 2582
Size: 3475 1286 1156 3369
Fragments arranged by size:
3475 3369 1286 1156
ApoI r'AATT_y
Cuts at: 1493 6399 6595 6821 6973 9082 1493
Size: 4906 196 226 152 2109 1697
Fragments arranged by size:
4906 2109 1697 226 196 152
AseI AT'TA_AT
Cuts at: 160 1032 7840 160
Size: 872 6808 1606
Fragments arranged by size:
6808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9286
AvaI C'yCGr_G
Cuts at: 1870 1943 2913 3714 4411 5409 5625 6276 6549
Size: 73 970 801 697 998 216 651 273
Cuts at: 6549 6611 1870
Size: 62 4545
Fragments arranged by size:
4545 998 970 801 697 651 273 216
73 62
BamHI G'GATC_C
Cuts at: 4875 6616 6983 4875
Size: 1741 367 7178
Fragments arranged by size:
7178 1741 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 2350 2690 2833 3131
Size: 325 217 627 392 171 340 143 298
Cuts at: 3131 3811 4772 4937 5240 5384 6607 8062 618
Size: 680 961 165 303 144 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 961 680 627 392 340
325 303 298 217 171 165 144 143
BanII G_rGCy'C
Cuts at: 1391 2565 2789 3030 3193 3227 3366 3667 3705
Size: 1174 224 241 163 34 139 301 38
Cuts at: 3705 4100 4418 4636 4756 4764 4795 4907 4957
Size: 395 318 218 120 8 31 112 50
Cuts at: 4957 4968 5084 5600 5632 5685 5709 5728 5939
Size: 11 116 516 32 53 24 19 211
Cuts at: 5939 6165 6556 6605 1391
Size: 226 391 49 4072
Fragments arranged by size:
4072 1174 516 395 391 318 301 241
226 224 218 211 163 139 120 116
112 53 50 49 38 34 32 31
24 19 11 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 8315
BbvCI CC'TCA_GC
Cuts at: 2356 2988 3390 3945 4509 4988 2356
Size: 632 402 555 564 479 6654
Fragments arranged by size:
6654 632 564 555 479 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5862 5896 7477 7511 1903
Size: 34 3925 34 1581 34 3678
Fragments arranged by size:
3925 3678 1581 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2085 7178 8388 2085
Size: 5093 1210 2983
Fragments arranged by size:
5093 2983 1210
BclI T'GATC_A
Cuts at: 1548 3586 1548
Size: 2038 7248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2940 3489 4911
Size: 107 122 71 631 605 1268 549 1422
Cuts at: 4911 5732 5792 6287 7897 136
Size: 821 60 495 1610 1525
Fragments arranged by size:
1610 1525 1422 1268 821 631 605 549
495 122 107 71 60
BglII A'GATC_T
Cuts at: 5815 9281 5815
Size: 3466 5820
BlpI GC'TnA_GC
Cuts at: 2559 6703 2559
Size: 4144 5142
Bme1580I G_kGCm'C
Cuts at: 1790 2227 2355 2433 2586 2715 3217 3692 4418
Size: 437 128 78 153 129 502 475 726
Cuts at: 4418 4636 4756 4795 4957 4968 5084 5278 5387
Size: 218 120 39 162 11 116 194 109
Cuts at: 5387 5410 5728 5939 6061 6250 7347 8503 1790
Size: 23 318 211 122 189 1097 1156 2573
Fragments arranged by size:
2573 1156 1097 726 502 475 437 318
218 211 194 189 162 153 129 128
122 120 116 109 78 39 23 11
BmgBI CAC'GTC
Cuts at: 1318 5175 1318
Size: 3857 5429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3788 4026 4048
Size: 416 53 83 399 24 2371 238 22
Cuts at: 4048 4758 4951 7017 7975 442
Size: 710 193 2066 958 1753
Fragments arranged by size:
2371 2066 1753 958 710 416 399 238
193 83 53 24 22
BmtI G_CTAG'C
Cuts at: 1056 2300 1056
Size: 1244 8042
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6513 6564 6513
Size: 51 9235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2634 2803 2874 3464 3542 3810 4145 4292
Size: 1395 169 71 590 78 268 335 147
Cuts at: 4292 4373 4574 4625 5079 5162 5579 5696 6453
Size: 81 201 51 454 83 417 117 757
Cuts at: 6453 7946 1239
Size: 1493 2579
Fragments arranged by size:
2579 1493 1395 757 590 454 417 335
268 201 169 147 117 83 81 78
71 51
Bpu10I CC'TnA_GC
Cuts at: 2031 2356 2988 3334 3390 3758 3945 4509 4988
Size: 325 632 346 56 368 187 564 479
Cuts at: 4988 5085 5361 5703 2031
Size: 97 276 342 5614
Fragments arranged by size:
5614 632 564 479 368 346 342 325
276 187 97 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3865 5353 5572 5915 6089 7411 8276 8574
Size: 1496 1488 219 343 174 1322 865 298
Cuts at: 8574 2369
Size: 3081
Fragments arranged by size:
3081 1496 1488 1322 865 343 298 219
174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5769 5841 7949 882
Size: 617 4270 72 2108 2219
Fragments arranged by size:
4270 2219 2108 617 72
BsaAI yAC'GTr
Cuts at: 493 1204 4351 5194 5323 5539 6583 7077 493
Size: 711 3147 843 129 216 1044 494 2702
Fragments arranged by size:
3147 2702 1044 843 711 494 216 129
BsaBI GATnn'nnATC
Cuts at: 9280 9280
Size: 9286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5369 6390 7093 7475
Size: 53 83 186 936 3836 1021 703 382
Cuts at: 7475 8110 275
Size: 635 1451
Fragments arranged by size:
3836 1451 1021 936 703 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2826 2871 2957 3056 5433 7719 8117 8391 8538
Size: 45 86 99 2377 2286 398 274 147
Cuts at: 8538 8949 2826
Size: 411 3163
Fragments arranged by size:
3163 2377 2286 411 398 274 147 99
86 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3983 4013 5733 5763 1178
Size: 30 165 30 2580 30 1720 30 4701
Fragments arranged by size:
4701 2580 1720 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2693 3105 3108 3111 3114 3117 3216 3713
Size: 1507 412 3 3 3 3 99 497
Cuts at: 3713 4004 4133 4166 4439 4571 4885 4910 5693
Size: 291 129 33 273 132 314 25 783
Cuts at: 5693 5903 6383 6386 1186
Size: 210 480 3 4086
Fragments arranged by size:
4086 1507 783 497 480 412 314 291
273 210 132 129 99 33 25 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2660 2686 3031 4093 4259 4285 4706 4816 4982
Size: 26 345 1062 166 26 421 110 166
Cuts at: 4982 5006 5200 5277 5696 6126 6464 8489 8985
Size: 24 194 77 419 430 338 2025 496
Cuts at: 8985 2660
Size: 2961
Fragments arranged by size:
2961 2025 1062 496 430 421 419 345
338 194 166 166 110 77 26 26
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2312 2435 3551 4331 4373 4719
Size: 573 336 240 123 1116 780 42 346
Cuts at: 4719 5559 6191 1163
Size: 840 632 4258
Fragments arranged by size:
4258 1116 840 780 632 573 346 336
240 123 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6736 7497 8525 1540
Size: 275 186 4735 761 1028 2301
Fragments arranged by size:
4735 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2060 2565 2586 2932 3291 3366 3667 5180 5685
Size: 505 21 346 359 75 301 1513 505
Cuts at: 5685 5709 6061 6165 7347 7432 8503 2060
Size: 24 352 104 1182 85 1071 2843
Fragments arranged by size:
2843 1513 1182 1071 505 505 359 352
346 301 104 85 75 24 21
BsmI GAATG_Cn'
Cuts at: 3308 4170 4567 6797 6890 3308
Size: 862 397 2230 93 5704
Fragments arranged by size:
5704 2230 862 397 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3586 3586
Size: 9286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2227 2355 2433 2565 2586 2715
Size: 399 270 167 128 78 132 21 129
Cuts at: 2715 2789 2932 3030 3193 3217 3227 3291 3366
Size: 74 143 98 163 24 10 64 75
Cuts at: 3366 3667 3692 3705 4100 4418 4636 4756 4764
Size: 301 25 13 395 318 218 120 8
Cuts at: 4764 4795 4907 4957 4968 5084 5180 5278 5387
Size: 31 112 50 11 116 96 98 109
Cuts at: 5387 5410 5600 5632 5685 5709 5728 5939 6061
Size: 23 190 32 53 24 19 211 122
Cuts at: 6061 6165 6250 6556 6605 7347 7432 8503 1391
Size: 104 85 306 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 395 318 306 301
270 218 211 190 167 163 143 132
129 128 122 120 116 112 109 104
98 98 96 85 85 78 75 74
64 53 50 49 32 31 25 24
24 23 21 19 13 11 10 8
BspEI T'CCGG_A
Cuts at: 2826 3056 8949 2826
Size: 230 5893 3163
Fragments arranged by size:
5893 3163 230
BspHI T'CATG_A
Cuts at: 3904 7175 8905 3904
Size: 3271 1730 4285
Fragments arranged by size:
4285 3271 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2335 2522 3170 4353 4384 4600 5628
Size: 1358 133 187 648 1183 31 216 1028
Cuts at: 5628 5787 6641 844
Size: 159 854 3489
Fragments arranged by size:
3489 1358 1183 1028 854 648 216 187
159 133 31
BsrBI CCG'CTC
Cuts at: 1394 1823 2266 6235 7173 1394
Size: 429 443 3969 938 3507
Fragments arranged by size:
3969 3507 938 443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5038 7781 7955 65
Size: 1183 3790 2743 174 1396
Fragments arranged by size:
3790 2743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2490 4769 6311 7930 8117 1700
Size: 110 680 2279 1542 1619 187 2869
Fragments arranged by size:
2869 2279 1619 1542 680 187 110
BsrGI T'GTAC_A
Cuts at: 96 5740 96
Size: 5644 3642
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9286
BssSI C'ACGA_G
Cuts at: 6060 7346 8358 6060
Size: 1286 1012 6988
Fragments arranged by size:
6988 1286 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 2350 4186 4378 4399 4442 4937 794
Size: 1526 30 1836 192 21 43 495 5143
Fragments arranged by size:
5143 1836 1526 495 192 43 30 21
BstBI TT'CG_AA
Cuts at: 2007 8090 2007
Size: 6083 3203
BstEII G'GTnAC_C
Cuts at: 1184 5146 1184
Size: 3962 5324
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2234 2107
Size: 127 9159
BstYI r'GATC_y
Cuts at: 1972 3059 3868 4195 4875 5815 6382 6519 6616
Size: 1087 809 327 680 940 567 137 97
Cuts at: 6616 6983 7368 7385 8837 8923 8935 9281 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1087 940 809 680 567 385
367 346 327 137 97 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2676 2736 3071 3696 5687 2676
Size: 60 335 625 1991 6275
Fragments arranged by size:
6275 1991 625 335 60
BtgI C'CryG_G
Cuts at: 513 1065 2022 4907 5021 5183 5506 5601 5767
Size: 552 957 2885 114 162 323 95 166
Cuts at: 5767 5787 6186 6285 513
Size: 20 399 99 3514
Fragments arranged by size:
3514 2885 957 552 399 323 166 162
114 99 95 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8547
Fragments arranged by size:
8547 683 56
BtsI GCAGTG_nn'
Cuts at: 2621 3752 3818 4004 4164 4175 4211 4379 4409
Size: 1131 66 186 160 11 36 168 30
Cuts at: 4409 4951 6802 7596 7616 2621
Size: 542 1851 794 20 4291
Fragments arranged by size:
4291 1851 1131 794 542 186 168 160
66 36 30 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9251
DraI TTT'AAA
Cuts at: 6590 6590
Size: 9286
DraIII CAC_nnn'GTG
Cuts at: 3284 3284
Size: 9286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8293 809
Size: 507 6977 1802
Fragments arranged by size:
6977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2261 2492 3978
Size: 54 1425 50 275 18 431 231 1486
Cuts at: 3978 5024 5325 5784 5869 6288 6507 6733 7622
Size: 1046 301 459 85 419 219 226 889
Cuts at: 7622 9251 8
Size: 1629 43
Fragments arranged by size:
1629 1486 1425 1046 889 459 431 419
301 275 231 226 219 85 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6733 1537
Size: 275 4921 4090
Fragments arranged by size:
4921 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 3414 3555 3741 4143 5070 5906 6256
Size: 896 1165 141 186 402 927 836 350
Cuts at: 6256 6433 7216 1353
Size: 177 783 3423
Fragments arranged by size:
3423 1165 927 896 836 783 402 350
186 177 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7873 8247 8393 387
Size: 1064 138 6284 374 146 1280
Fragments arranged by size:
6284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2563 3364 3665 5683 5707 6163 2563
Size: 801 301 2018 24 456 5686
Fragments arranged by size:
5686 2018 801 456 301 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2634 2803 2820 2874 3138 3420 3464
Size: 393 1002 169 17 54 264 282 44
Cuts at: 3464 3542 3810 3839 4145 4292 4373 4574 4625
Size: 78 268 29 306 147 81 201 51
Cuts at: 4625 5079 5162 5441 5579 5696 5840 5961 6453
Size: 454 83 279 138 117 144 121 492
Cuts at: 6453 7349 7946 8733 9001 1239
Size: 896 597 787 268 1524
Fragments arranged by size:
1524 1002 896 787 597 492 454 393
306 282 279 268 268 264 201 169
147 144 138 121 117 83 81 78
54 51 44 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2727 3314 3806 4529 4892 2727
Size: 587 492 723 363 7121
Fragments arranged by size:
7121 723 587 492 363
EcoO109I rG'GnC_Cy
Cuts at: 2593 2732 2902 2909 3491 3565 4021 4543 4633
Size: 139 170 7 582 74 456 522 90
Cuts at: 4633 4753 4792 4953 4964 4965 5080 5081 5112
Size: 120 39 161 11 1 115 1 31
Cuts at: 5112 5490 5725 5935 5936 2593
Size: 378 235 210 1 5943
Fragments arranged by size:
5943 582 522 456 378 235 210 170
161 139 120 115 90 74 39 31
11 7 1 1 1
EcoRI G'AATT_C
Cuts at: 6595 6973 6595
Size: 378 8908
EcoRV GAT'ATC
Cuts at: 4588 4588
Size: 9286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3278 3584 5992 3278
Size: 306 2408 6572
Fragments arranged by size:
6572 2408 306
FspI TGC'GCA
Cuts at: 7792 7792
Size: 9286
HaeII r_GCGC'y
Cuts at: 1678 2687 2853 4509 4525 4651 8433 1678
Size: 1009 166 1656 16 126 3782 2531
Fragments arranged by size:
3782 2531 1656 1009 166 126 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2125 2157 2367 2399 3615
Size: 32 135 32 553 32 210 32 1216
Cuts at: 3615 3647 3938 3970 4448 4480 7927 7959 8001
Size: 32 291 32 478 32 3447 32 42
Cuts at: 8001 8033 1373
Size: 32 2626
Fragments arranged by size:
3447 2626 1216 553 478 291 210 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6630 6876 669
Size: 988 281 4692 246 3079
Fragments arranged by size:
4692 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3293 4438 748
Size: 2545 1145 5596
Fragments arranged by size:
5596 2545 1145
HpaI GTT'AAC
Cuts at: 6876 6876
Size: 9286
KpnI G_GTAC'C
Cuts at: 2183 2694 2837 3135 6611 2183
Size: 511 143 298 3476 4858
Fragments arranged by size:
4858 3476 511 298 143
MfeI C'AATT_G
Cuts at: 6885 6885
Size: 9286
MluI A'CGCG_T
Cuts at: 9162 9162
Size: 9286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2584 3533 3941 4365 8400 8584 1305
Size: 1279 949 408 424 4035 184 2007
Fragments arranged by size:
4035 2007 1279 949 424 408 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3980 5327 6509 10
Size: 54 1425 343 2148 1347 1182 2787
Fragments arranged by size:
2787 2148 1425 1347 1182 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2386 2464 3594 4238 4669 5159 7244
Size: 648 1220 78 1130 644 431 490 2085
Cuts at: 7244 7603 7762 518
Size: 359 159 2042
Fragments arranged by size:
2085 2042 1220 1130 648 644 490 431
359 159 78
MspA1I CmG'CkG
Cuts at: 1606 2360 2545 3520 3886 4369 4432 4600 4842
Size: 754 185 975 366 483 63 168 242
Cuts at: 4842 4854 5284 5653 5746 6021 6130 6702 7379
Size: 12 430 369 93 275 109 572 677
Cuts at: 7379 8527 8772 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 975 754 677 572 483 430
369 366 275 245 242 185 168 109
93 63 12
NaeI GCC'GGC
Cuts at: 4771 6313 4771
Size: 1542 7744
NcoI C'CATG_G
Cuts at: 513 1065 2022 5601 6186 513
Size: 552 957 3579 585 3613
Fragments arranged by size:
3613 3579 957 585 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9286
NgoMIV G'CCGG_C
Cuts at: 4769 6311 4769
Size: 1542 7744
NheI G'CTAG_C
Cuts at: 1052 2296 1052
Size: 1244 8042
NotI GC'GGCC_GC
Cuts at: 6733 6733
Size: 9286
NspI r_CATG'y
Cuts at: 1132 1229 6311 6644 8189 1132
Size: 97 5082 333 1545 2229
Fragments arranged by size:
5082 2229 1545 333 97
PasI CC'CwG_GG
Cuts at: 1348 2399 2553 3227 4418 5015 5229 5354 6485
Size: 1051 154 674 1191 597 214 125 1131
Cuts at: 6485 1348
Size: 4149
Fragments arranged by size:
4149 1191 1131 1051 674 597 214 154
125
PciI A'CATG_T
Cuts at: 8185 8185
Size: 9286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 4038 4060 5354 1108
Size: 1020 1910 22 1294 5040
Fragments arranged by size:
5040 1910 1294 1020 22
PfoI T'CCnGG_A
Cuts at: 4515 4779 5673 5751 6210 4515
Size: 264 894 78 459 7591
Fragments arranged by size:
7591 894 459 264 78
PmeI GTTT'AAAC
Cuts at: 6590 6590
Size: 9286
PmlI CAC'GTG
Cuts at: 4351 5194 4351
Size: 843 8443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7355 7387 8868 8900 7355
Size: 32 1481 32 7741
Fragments arranged by size:
7741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2732 4021 2732
Size: 1289 7997
PshAI GACnn'nnGTC
Cuts at: 1613 3804 1613
Size: 2191 7095
PsiI TTA'TAA
Cuts at: 6856 6856
Size: 9286
PspOMI G'GGCC_C
Cuts at: 4414 4632 4752 4791 4953 4964 5080 5724 5935
Size: 218 120 39 162 11 116 644 211
Cuts at: 5935 4414
Size: 7765
Fragments arranged by size:
7765 644 218 211 162 120 116 39
11
PspXI vC'TCGA_Gb
Cuts at: 6549 6549
Size: 9286
PstI C_TGCA'G
Cuts at: 830 2346 3181 3999 4182 4218 4476 5637 6081
Size: 1516 835 818 183 36 258 1161 444
Cuts at: 6081 6638 830
Size: 557 3478
Fragments arranged by size:
3478 1516 1161 835 818 557 444 258
183 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9286
PvuII CAG'CTG
Cuts at: 2360 4432 5653 5746 6130 2360
Size: 2072 1221 93 384 5516
Fragments arranged by size:
5516 2072 1221 384 93
SacI G_AGCT'C
Cuts at: 2565 3366 3667 5685 5709 6165 2565
Size: 801 301 2018 24 456 5686
Fragments arranged by size:
5686 2018 801 456 301 24
SalI G'TCGA_C
Cuts at: 1936 6628 1936
Size: 4692 4594
SanDI GG'GwC_CC
Cuts at: 4021 4021
Size: 9286
SapI GCTCTTCn'nnn_
Cuts at: 6256 6256
Size: 9286
SbfI CC_TGCA'GG
Cuts at: 4476 6638 4476
Size: 2162 7124
ScaI AGT'ACT
Cuts at: 7534 9246 7534
Size: 1712 7574
SexAI A'CCwGG_T
Cuts at: 3127 3309 3127
Size: 182 9104
SfcI C'TryA_G
Cuts at: 826 1045 2342 3177 3414 3770 3995 4178 4214
Size: 219 1297 835 237 356 225 183 36
Cuts at: 4214 4472 4643 5633 6077 6634 7769 8450 8641
Size: 258 171 990 444 557 1135 681 191
Cuts at: 8641 826
Size: 1471
Fragments arranged by size:
1471 1297 1135 990 835 681 557 444
356 258 237 225 219 191 183 171
36
SfiI GGCCn_nnn'nGGCC
Cuts at: 3489 6287 3489
Size: 2798 6488
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9286
SmaI CCC'GGG
Cuts at: 1872 5411 6278 6613 1872
Size: 3539 867 335 4545
Fragments arranged by size:
4545 3539 867 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2384 3844 5332 5587 5930 6104
Size: 197 926 441 1460 1488 255 343 174
Cuts at: 6104 6549 7390 8291 8553 820
Size: 445 841 901 262 1553
Fragments arranged by size:
1553 1488 1460 926 901 841 445 441
343 262 255 197 174
SnaBI TAC'GTA
Cuts at: 493 6583 7077 493
Size: 6090 494 2702
Fragments arranged by size:
6090 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9286
SphI G_CATG'C
Cuts at: 1132 6311 6644 1132
Size: 5179 333 3774
Fragments arranged by size:
5179 3774 333
SspI AAT'ATT
Cuts at: 5 52 7210 5
Size: 47 7158 2081
Fragments arranged by size:
7158 2081 47
StuI AGG'CCT
Cuts at: 1517 2814 3456 3763 5139 5975 6377 1517
Size: 1297 642 307 1376 836 402 4426
Fragments arranged by size:
4426 1376 1297 836 642 402 307
StyI C'CwwG_G
Cuts at: 513 1065 2022 2202 3262 4084 4279 5601 5728
Size: 552 957 180 1060 822 195 1322 127
Cuts at: 5728 6186 6226 6725 513
Size: 458 40 499 3074
Fragments arranged by size:
3074 1322 1060 957 822 552 499 458
195 180 127 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4663 5486 7313 7330 7483 7668 8113
Size: 1255 3083 823 1827 17 153 185 445
Cuts at: 8113 325
Size: 1498
Fragments arranged by size:
3083 1827 1498 1255 823 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2618 5740 7532
Size: 274 80 33 51 1443 641 3122 1792
Cuts at: 7532 9244 96
Size: 1712 138
Fragments arranged by size:
3122 1792 1712 1443 641 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3983 4015 6229 6261 9246 9278 3983
Size: 32 2214 32 2985 32 3991
Fragments arranged by size:
3991 2985 2214 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6540 1316
Size: 5224 4062
XbaI T'CTAG_A
Cuts at: 6622 6622
Size: 9286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3260 4120 4267 5158 5226 3260
Size: 860 147 891 68 7320
Fragments arranged by size:
7320 891 860 147 68
XhoI C'TCGA_G
Cuts at: 1943 6549 1943
Size: 4606 4680
XmaI C'CCGG_G
Cuts at: 1870 5409 6276 6611 1870
Size: 3539 867 335 4545
Fragments arranged by size:
4545 3539 867 335
XmnI GAAnn'nnTTC
Cuts at: 5418 7415 5418
Size: 1997 7289
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6391 7094 276
Size: 53 83 186 936 4857 703 2468
Fragments arranged by size:
4857 2468 936 703 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
FseI FspAI KasI NarI NruI NsiI PacI PsrI
RsrII SacII SfoI SrfI SwaI