Restriction map for
pFN21ASDF0106
(Circular) (Six-base) MAPSORT of: pFN21ASDF0106.seq Check: 9421 from: 1 to: 5827
pFN21ASDF0106 5827 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 2934 3148 2934
Size: 214 5613
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3002 3890 5274 5542 1632
Size: 1370 888 1384 268 1917
Fragments arranged by size:
1917 1384 1370 888 268
AflII C'TTAA_G
Cuts at: 820 1017 2374 820
Size: 197 1357 4273
Fragments arranged by size:
4273 1357 197
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 2265 4658 2265
Size: 2393 3434
AhdI GACnn_n'nnGTC
Cuts at: 2530 4556 2530
Size: 2026 3801
AleI CACnn'nnGTG
Cuts at: 1166 2877 1166
Size: 1711 4116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 2494 3570 5142 2494
Size: 1076 1572 3179
Fragments arranged by size:
3179 1572 1076
ApaI G_GGCC'C
Cuts at: 2621 2851 2621
Size: 230 5597
ApaLI G'TGCA_C
Cuts at: 3884 5040 3884
Size: 1156 4671
ApoI r'AATT_y
Cuts at: 1493 3136 3362 3514 5623 1493
Size: 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2410 2506 2614 3152 1870
Size: 73 467 96 108 538 4545
Fragments arranged by size:
4545 538 467 108 96 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2934 3068 3148 4603 618
Size: 325 217 627 1147 134 80 1455 1842
Fragments arranged by size:
1842 1455 1147 627 325 217 134 80
BanII G_rGCy'C
Cuts at: 1391 2240 2342 2621 2822 2851 3146 1391
Size: 849 102 279 201 29 295 4072
Fragments arranged by size:
4072 849 295 279 201 102 29
BbeI G_GCGC'C
Cuts at: 3072 3072
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2680 3098 928
Size: 971 781 418 3657
Fragments arranged by size:
3657 971 781 418
BbvCI CC'TCA_GC
Cuts at: 2405 2775 2405
Size: 370 5457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2690 2724 4018 4052 1903
Size: 34 753 34 1294 34 3678
Fragments arranged by size:
3678 1294 753 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2251 2580 2771
Size: 107 122 71 631 605 579 329 191
Cuts at: 2771 2846 2946 4438 136
Size: 75 100 1492 1525
Fragments arranged by size:
1525 1492 631 605 579 329 191 122
107 100 75 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3244 3244
Size: 5827
Bme1580I G_kGCm'C
Cuts at: 1790 2621 2851 2997 3888 5044 1790
Size: 831 230 146 891 1156 2573
Fragments arranged by size:
2573 1156 891 831 230 146
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2818 3558 4516
Size: 416 53 83 399 24 1401 740 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1401 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2178 2268 2374 2481 2614 2789 2827 4487
Size: 939 90 106 107 133 175 38 1660
Cuts at: 4487 1239
Size: 2579
Fragments arranged by size:
2579 1660 939 175 133 107 106 90
38
Bpu10I CC'TnA_GC
Cuts at: 2405 2775 2405
Size: 370 5457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2274 3094 4490 882
Size: 617 775 820 1396 2219
Fragments arranged by size:
2219 1396 820 775 617
BsaAI yAC'GTr
Cuts at: 493 1204 3124 3618 493
Size: 711 1920 494 2702
Fragments arranged by size:
2702 1920 711 494
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3069 3634 4016 4651
Size: 53 83 186 936 1536 565 382 635
Cuts at: 4651 275
Size: 1451
Fragments arranged by size:
1536 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2265 4260 4658 4932 5079 5490 2265
Size: 1995 398 274 147 411 2602
Fragments arranged by size:
2602 1995 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2918 2948 1178
Size: 30 165 30 1515 30 4057
Fragments arranged by size:
4057 1515 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2792 1186
Size: 1468 138 4221
Fragments arranged by size:
4221 1468 138
BseYI C'CCAG_C
Cuts at: 2636 2807 5030 5526 2636
Size: 171 2223 496 2937
Fragments arranged by size:
2937 2223 496 171
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2908 1163
Size: 573 1172 4082
Fragments arranged by size:
4082 1172 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2575 3277 4038 5066 1540
Size: 275 186 574 702 761 1028 2301
Fragments arranged by size:
2301 1028 761 702 574 275 186
BsiHKAI G_wGCw'C
Cuts at: 3888 3973 5044 3888
Size: 85 1071 4671
Fragments arranged by size:
4671 1071 85
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
BsmBI CGTCTCn'nnnn_
Cuts at: 2286 2286
Size: 5827
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2342 2621 2822 2851 2997 3146
Size: 399 450 102 279 201 29 146 149
Cuts at: 3146 3888 3973 5044 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 279 201 149
146 102 85 29
BspEI T'CCGG_A
Cuts at: 5490 5490
Size: 5827
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 844
Size: 2338 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2125 3714 1394
Size: 429 302 1589 3507
Fragments arranged by size:
3507 1589 429 302
BsrDI GCAATG_nn'
Cuts at: 65 1248 4322 4496 65
Size: 1183 3074 174 1396
Fragments arranged by size:
3074 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2265 2417 2564 2931 4471 4658 1700
Size: 110 455 152 147 367 1540 187 2869
Fragments arranged by size:
2869 1540 455 367 187 152 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5827
BstBI TT'CG_AA
Cuts at: 2007 4631 2007
Size: 2624 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2058 2851 2058
Size: 793 5034
BstYI r'GATC_y
Cuts at: 1972 2226 3157 3524 3909 3926 5378 5464 5476
Size: 254 931 367 385 17 1452 86 12
Cuts at: 5476 5822 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 931 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4318
Fragments arranged by size:
4318 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2884 487
Size: 683 56 1658 3430
Fragments arranged by size:
3430 1658 683 56
BtsI GCAGTG_nn'
Cuts at: 3343 4137 4157 3343
Size: 794 20 5013
Fragments arranged by size:
5013 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5792
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2684 4834 809
Size: 507 1368 2150 1802
Fragments arranged by size:
2150 1802 1368 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2118 2172
Size: 54 1425 50 275 18 213 75 54
Cuts at: 2172 2469 2572 2853 3274 4163 5792 8
Size: 297 103 281 421 889 1629 43
Fragments arranged by size:
1629 1425 889 421 297 281 275 213
103 75 54 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2572 3274 1537
Size: 275 760 702 4090
Fragments arranged by size:
4090 760 702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3757 1353
Size: 2404 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 4414 4788 4934 387
Size: 1064 138 630 2195 374 146 1280
Fragments arranged by size:
2195 1280 1064 630 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2178 2268 2374 2481 2614 2789 2827
Size: 393 546 90 106 107 133 175 38
Cuts at: 2827 3002 3890 4487 5274 5542 1239
Size: 175 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 597 546 393 268 175
175 133 107 106 90 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2374 2672 2374
Size: 298 5529
EcoO109I rG'GnC_Cy
Cuts at: 2379 2618 2847 2848 2379
Size: 239 229 1 5358
Fragments arranged by size:
5358 239 229 1
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FseI GG_CCGG'CC
Cuts at: 2568 2568
Size: 5827
FspI TGC'GCA
Cuts at: 2918 4333 2918
Size: 1415 4412
FspAI rTGC'GCAy
Cuts at: 2918 2918
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2057 3072 4974 1678
Size: 379 1015 1902 2531
Fragments arranged by size:
2531 1902 1015 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2151 2183 2351 2383 2516
Size: 32 135 32 579 32 168 32 133
Cuts at: 2516 2548 2663 2695 4468 4500 4542 4574 1373
Size: 32 115 32 1773 32 42 32 2626
Fragments arranged by size:
2626 1773 579 168 135 133 115 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2031 3171 3417 669
Size: 988 281 93 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246 93
HindIII A'AGCT_T
Cuts at: 748 3045 748
Size: 2297 3530
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 3068 3068
Size: 5827
KpnI G_GTAC'C
Cuts at: 2938 3152 2938
Size: 214 5613
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2855 10
Size: 54 1425 343 342 681 2982
Fragments arranged by size:
2982 1425 681 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2168 2877 3785 4144 4303 518
Size: 648 939 63 709 908 359 159 2042
Fragments arranged by size:
2042 939 908 709 648 359 159 63
MspA1I CmG'CkG
Cuts at: 1606 2260 2519 3243 3920 5068 5313 1606
Size: 654 259 724 677 1148 245 2120
Fragments arranged by size:
2120 1148 724 677 654 259 245
NaeI GCC'GGC
Cuts at: 2419 2566 2419
Size: 147 5680
NarI GG'CG_CC
Cuts at: 3069 3069
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 4318
Fragments arranged by size:
4318 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2417 2564 2417
Size: 147 5680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NruI TCG'CGA
Cuts at: 2712 2903 2712
Size: 191 5636
NspI r_CATG'y
Cuts at: 1132 1229 2203 2210 2502 3185 4730 1132
Size: 97 974 7 292 683 1545 2229
Fragments arranged by size:
2229 1545 974 683 292 97 7
PasI CC'CwG_GG
Cuts at: 1348 2058 1348
Size: 710 5117
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2494 1108
Size: 1386 4441
PfoI T'CCnGG_A
Cuts at: 2667 2922 2667
Size: 255 5572
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2379 2379
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2617 2847 2617
Size: 230 5597
PstI C_TGCA'G
Cuts at: 830 2259 2703 3179 830
Size: 1429 444 476 3478
Fragments arranged by size:
3478 1429 476 444
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5827
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SfcI C'TryA_G
Cuts at: 826 1045 2255 2699 3175 4310 4991 5182 826
Size: 219 1210 444 476 1135 681 191 1471
Fragments arranged by size:
1471 1210 1135 681 476 444 219 191
SfoI GGC'GCC
Cuts at: 3070 3070
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 2508 2616 3154 1872
Size: 636 108 538 4545
Fragments arranged by size:
4545 636 538 108
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2374 3931 4832 5094 820
Size: 197 926 431 1557 901 262 1553
Fragments arranged by size:
1557 1553 926 901 431 262 197
SnaBI TAC'GTA
Cuts at: 493 3124 3618 493
Size: 2631 494 2702
Fragments arranged by size:
2702 2631 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 2203 2210 3185 1132
Size: 1071 7 975 3774
Fragments arranged by size:
3774 1071 975 7
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5827
StyI C'CwwG_G
Cuts at: 513 1065 2022 2136 2310 3266 513
Size: 552 957 114 174 956 3074
Fragments arranged by size:
3074 957 956 552 174 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5787 5819 5787
Size: 32 5795
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 2638
Size: 5827
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 2506 2614 3152 1870
Size: 636 108 538 4545
Fragments arranged by size:
4545 636 538 108
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI