Restriction map for
pFN21ASDF0044
(Circular) (Six-base) MAPSORT of: pFN21ASDF0044.seq Check: 1637 from: 1 to: 7696
pFN21ASDF0044 7696 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2164 2346 2803 3102 3233 3550 4039 2164
Size: 182 457 299 131 317 489 5821
Fragments arranged by size:
5821 489 457 317 299 182 131
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3132 5506 278
Size: 53 83 186 936 1596 2374 2468
Fragments arranged by size:
2468 2374 1596 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5039 1937
Size: 3102 4594
Acc65I G'GTAC_C
Cuts at: 5017 5017
Size: 7696
AclI AA'CG_TT
Cuts at: 1563 5823 6196 1563
Size: 4260 373 3063
Fragments arranged by size:
4260 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3589 5759 7143 7411 1632
Size: 1957 2170 1384 268 1917
Fragments arranged by size:
2170 1957 1917 1384 268
AfeI AGC'GCT
Cuts at: 2684 2684
Size: 7696
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7499
AflIII A'CryG_T
Cuts at: 1203 6595 7572 1203
Size: 5392 977 1327
Fragments arranged by size:
5392 1327 977
AgeI A'CCGG_T
Cuts at: 6527 6527
Size: 7696
AhdI GACnn_n'nnGTC
Cuts at: 6425 6425
Size: 7696
AleI CACnn'nnGTG
Cuts at: 1166 2477 2663 3654 4184 1166
Size: 1311 186 991 530 4678
Fragments arranged by size:
4678 1311 991 530 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3672 1184
Size: 2488 5208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 3611 3643 4154 4186 2171
Size: 32 1408 32 511 32 5681
Fragments arranged by size:
5681 1408 511 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2827 2863 2953 3517 3565 4768 5439 7011 2827
Size: 36 90 564 48 1203 671 1572 3512
Fragments arranged by size:
3512 1572 1203 671 564 90 48 36
ApaI G_GGCC'C
Cuts at: 3040 3463 4706 3040
Size: 423 1243 6030
Fragments arranged by size:
6030 1243 423
ApaLI G'TGCA_C
Cuts at: 5753 6909 5753
Size: 1156 6540
ApoI r'AATT_y
Cuts at: 1493 4853 5005 5231 5383 7492 1493
Size: 3360 152 226 152 2109 1697
Fragments arranged by size:
3360 2109 1697 226 152 152
AseI AT'TA_AT
Cuts at: 160 1032 6250 160
Size: 872 5218 1606
Fragments arranged by size:
5218 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7696
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2117 2193 2310 2426 3350 3389
Size: 73 126 48 76 117 116 924 39
Cuts at: 3389 4869 5021 1870
Size: 1480 152 4545
Fragments arranged by size:
4545 1480 924 152 126 117 116 76
73 48 39
BamHI G'GATC_C
Cuts at: 5026 5393 5026
Size: 367 7329
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 2314 2735 2784 3255
Size: 325 217 627 410 117 421 49 471
Cuts at: 3255 4217 4690 4739 4750 4888 4899 5017 6472
Size: 962 473 49 11 138 11 118 1455
Cuts at: 6472 618
Size: 1842
Fragments arranged by size:
1842 1455 962 627 473 471 421 410
325 217 138 118 117 49 49 11
11
BanII G_rGCy'C
Cuts at: 1391 2208 2427 2714 3040 3065 3221 3398 3463
Size: 817 219 287 326 25 156 177 65
Cuts at: 3463 3920 4275 4608 4706 5015 1391
Size: 457 355 333 98 309 4072
Fragments arranged by size:
4072 817 457 355 333 326 309 287
219 177 156 98 65 25
BbeI G_GCGC'C
Cuts at: 4694 4754 4892 4694
Size: 60 138 7498
Fragments arranged by size:
7498 138 60
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6725
BbvCI CC'TCA_GC
Cuts at: 2135 2135
Size: 7696
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2288 2322 4592 4626 5887 5921 1903
Size: 34 351 34 2270 34 1261 34 3678
Fragments arranged by size:
3678 2270 1261 351 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5588 6798 5588
Size: 1210 6486
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3472 4379 4748
Size: 107 122 71 631 605 1800 907 369
Cuts at: 4748 4897 6307 136
Size: 149 1410 1525
Fragments arranged by size:
1800 1525 1410 907 631 605 369 149
122 107 71
BglII A'GATC_T
Cuts at: 7691 7691
Size: 7696
BlpI GC'TnA_GC
Cuts at: 2679 4517 5113 2679
Size: 1838 596 5262
Fragments arranged by size:
5262 1838 596
Bme1580I G_kGCm'C
Cuts at: 1790 2319 2521 2740 3040 3258 3390 3463 3645
Size: 529 202 219 300 218 132 73 182
Cuts at: 3645 3661 4220 4651 4706 4717 4902 5757 6913
Size: 16 559 431 55 11 185 855 1156
Cuts at: 6913 1790
Size: 2573
Fragments arranged by size:
2573 1156 855 559 529 431 300 219
218 202 185 182 132 73 55 16
11
BmgBI CAC'GTC
Cuts at: 1318 2475 4561 1318
Size: 1157 2086 4453
Fragments arranged by size:
4453 2086 1157
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3923 4745 5427
Size: 416 53 83 399 24 2506 822 682
Cuts at: 5427 6385 442
Size: 958 1753
Fragments arranged by size:
2506 1753 958 822 682 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7696
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2206 2614 2206
Size: 408 7288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2439 3083 3194 3317 3332 3446 3548
Size: 1088 112 644 111 123 15 114 102
Cuts at: 3548 3764 3783 3857 4055 4889 6356 1239
Size: 216 19 74 198 834 1467 2579
Fragments arranged by size:
2579 1467 1088 834 644 216 198 123
114 112 111 102 74 19 15
Bpu10I CC'TnA_GC
Cuts at: 2135 2202 2387 3059 2135
Size: 67 185 672 6772
Fragments arranged by size:
6772 672 185 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5821 6686 6984 5821
Size: 865 298 6533
Fragments arranged by size:
6533 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3833 3882 4868 6359 882
Size: 617 2334 49 986 1491 2219
Fragments arranged by size:
2334 2219 1491 986 617 49
BsaAI yAC'GTr
Cuts at: 493 1204 2524 3379 4993 5487 493
Size: 711 1320 855 1614 494 2702
Fragments arranged by size:
2702 1614 1320 855 711 494
BsaBI GATnn'nnATC
Cuts at: 4151 7690 4151
Size: 3539 4157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2772 3129 3972 4691
Size: 53 83 186 936 1239 357 843 719
Cuts at: 4691 4751 4889 5503 5885 6520 275
Size: 60 138 614 382 635 1451
Fragments arranged by size:
1451 1239 936 843 719 635 614 382
357 186 138 83 60 53
BsaWI w'CCGG_w
Cuts at: 6129 6527 6801 6948 7359 6129
Size: 398 274 147 411 6466
Fragments arranged by size:
6466 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2429 2459 3461
Size: 30 165 30 768 30 228 30 1002
Cuts at: 3461 3491 3524 3554 3611 3641 1178
Size: 30 33 30 57 30 5233
Fragments arranged by size:
5233 1002 768 228 165 57 33 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2195 2810 3329 3332 3521 3545 4283
Size: 901 108 615 519 3 189 24 738
Cuts at: 4283 1186
Size: 4599
Fragments arranged by size:
4599 901 738 615 519 189 108 24
3
BseYI C'CCAG_C
Cuts at: 2160 2275 2707 3031 3170 3439 3445 3776 4021
Size: 115 432 324 139 269 6 331 245
Cuts at: 4021 4039 6899 7395 2160
Size: 18 2860 496 2461
Fragments arranged by size:
2860 2461 496 432 331 324 269 245
139 115 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2318 2654 3578 3887 1163
Size: 573 582 336 924 309 4972
Fragments arranged by size:
4972 924 582 573 336 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3879 4413 4747 5146 5907 6935
Size: 275 186 1878 534 334 399 761 1028
Cuts at: 6935 1540
Size: 2301
Fragments arranged by size:
2301 1878 1028 761 534 399 334 275
186
BsiHKAI G_wGCw'C
Cuts at: 2304 4275 4464 4608 5757 5842 6913 2304
Size: 1971 189 144 1149 85 1071 3087
Fragments arranged by size:
3087 1971 1149 1071 189 144 85
BsmI GAATG_Cn'
Cuts at: 2547 2907 5207 5300 2547
Size: 360 2300 93 4943
Fragments arranged by size:
4943 2300 360 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2585 2585
Size: 7696
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2304 2319 2427 2521 2714 2740
Size: 399 418 96 15 108 94 193 26
Cuts at: 2740 3040 3065 3221 3258 3390 3398 3463 3645
Size: 300 25 156 37 132 8 65 182
Cuts at: 3645 3661 3920 4220 4275 4464 4608 4651 4706
Size: 16 259 300 55 189 144 43 55
Cuts at: 4706 4717 4902 5015 5757 5842 6913 1391
Size: 11 185 113 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 418 399 300 300 259
193 189 185 182 156 144 132 113
108 96 94 85 65 55 55 43
37 26 25 16 15 11 8
BspEI T'CCGG_A
Cuts at: 7359 7359
Size: 7696
BspHI T'CATG_A
Cuts at: 2937 3688 3795 5585 7315 2937
Size: 751 107 1790 1730 3318
Fragments arranged by size:
3318 1790 1730 751 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2164 2346 2803 3102 3233 3550 4039 5051
Size: 1320 182 457 299 131 317 489 1012
Cuts at: 5051 844
Size: 3489
Fragments arranged by size:
3489 1320 1012 489 457 317 299 182
131
BsrBI CCG'CTC
Cuts at: 1394 1823 2185 2349 2391 3550 4477 4756 5583
Size: 429 362 164 42 1159 927 279 827
Cuts at: 5583 1394
Size: 3507
Fragments arranged by size:
3507 1159 927 827 429 362 279 164
42
BsrDI GCAATG_nn'
Cuts at: 65 1248 6191 6365 65
Size: 1183 4943 174 1396
Fragments arranged by size:
4943 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2112 2753 3012 4412 4596 6340 6527
Size: 110 302 641 259 1400 184 1744 187
Cuts at: 6527 1700
Size: 2869
Fragments arranged by size:
2869 1744 1400 641 302 259 187 184
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7696
BssHII G'CGCG_C
Cuts at: 1925 2921 1925
Size: 996 6700
BssSI C'ACGA_G
Cuts at: 4601 5756 6768 4601
Size: 1155 1012 5529
Fragments arranged by size:
5529 1155 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2953 3565 3758 4054 794
Size: 2159 612 193 296 4436
Fragments arranged by size:
4436 2159 612 296 193
BstBI TT'CG_AA
Cuts at: 2007 6500 2007
Size: 4493 3203
BstEII G'GTnAC_C
Cuts at: 1184 2279 1184
Size: 1095 6601
BstXI CCAn_nnnn'nTGG
Cuts at: 3784 4021 4735 3784
Size: 237 714 6745
Fragments arranged by size:
6745 714 237
BstYI r'GATC_y
Cuts at: 1972 2697 2802 5026 5393 5778 5795 7247 7333
Size: 725 105 2224 367 385 17 1452 86
Cuts at: 7333 7345 7691 1972
Size: 12 346 1977
Fragments arranged by size:
2224 1977 1452 725 385 367 346 105
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4708 4708
Size: 7696
BtgI C'CryG_G
Cuts at: 513 1065 2022 2034 2061 2326 2355 3186 4465
Size: 552 957 12 27 265 29 831 1279
Cuts at: 4465 4829 513
Size: 364 3380
Fragments arranged by size:
3380 1279 957 831 552 364 265 29
27 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 4012 4242 4325 4507 487
Size: 683 56 868 1918 230 83 182 3676
Fragments arranged by size:
3676 1918 868 683 230 182 83 56
BtsI GCAGTG_nn'
Cuts at: 2251 2673 3067 3434 3677 4215 4310 5212 6006
Size: 422 394 367 243 538 95 902 794
Cuts at: 6006 6026 2251
Size: 20 3921
Fragments arranged by size:
3921 902 794 538 422 394 367 243
95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7696
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4111 4146 529
Size: 35 3547 35 4079
Fragments arranged by size:
4079 3547 35 35
DraI TTT'AAA
Cuts at: 5000 5000
Size: 7696
DraIII CAC_nnn'GTG
Cuts at: 2246 2527 2566 4739 2246
Size: 281 39 2173 5203
Fragments arranged by size:
5203 2173 281 39
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4778 6703 809
Size: 507 3462 1925 1802
Fragments arranged by size:
3462 1925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2104 3829
Size: 54 1425 50 275 18 207 67 1725
Cuts at: 3829 3876 4410 5143 6032 7661 8
Size: 47 534 733 889 1629 43
Fragments arranged by size:
1725 1629 1425 889 733 534 275 207
67 54 50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3876 4410 5143 1537
Size: 275 2064 534 733 4090
Fragments arranged by size:
4090 2064 733 534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3138 4442 5626 1353
Size: 1160 625 1304 1184 3423
Fragments arranged by size:
3423 1304 1184 1160 625
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 6283 6657 6803 387
Size: 1064 138 2513 2181 374 146 1280
Fragments arranged by size:
2513 2181 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4273 4606 4273
Size: 333 7363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2439 3083 3194 3317 3332 3446
Size: 393 695 112 644 111 123 15 114
Cuts at: 3446 3548 3589 3764 3783 3857 4055 4889 5759
Size: 102 41 175 19 74 198 834 870
Cuts at: 5759 6356 7143 7411 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 870 834 787 695 644 597 393
268 198 175 123 114 112 111 102
74 41 19 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2792 3467 3527 4862 2792
Size: 675 60 1335 5626
Fragments arranged by size:
5626 1335 675 60
EcoO109I rG'GnC_Cy
Cuts at: 2066 3036 3037 3347 3460 3599 4318 4703 2066
Size: 970 1 310 113 139 719 385 5059
Fragments arranged by size:
5059 970 719 385 310 139 113 1
EcoRI G'AATT_C
Cuts at: 4853 5005 5383 4853
Size: 152 378 7166
Fragments arranged by size:
7166 378 152
FseI GG_CCGG'CC
Cuts at: 2116 2116
Size: 7696
FspI TGC'GCA
Cuts at: 3500 3569 6202 3500
Size: 69 2633 4994
Fragments arranged by size:
4994 2633 69
HaeII r_GCGC'y
Cuts at: 1678 2126 2142 2434 2652 2686 3293 3425 4002
Size: 448 16 292 218 34 607 132 577
Cuts at: 4002 4694 4754 4892 6843 1678
Size: 692 60 138 1951 2531
Fragments arranged by size:
2531 1951 692 607 577 448 292 218
138 132 60 34 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 6337 6369 6411 6443 1373
Size: 32 135 32 4765 32 42 32 2626
Fragments arranged by size:
4765 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 4555 5040 5286 669
Size: 988 281 1935 682 485 246 3079
Fragments arranged by size:
3079 1935 988 682 485 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7696
HpaI GTT'AAC
Cuts at: 5286 5286
Size: 7696
KasI G'GCGC_C
Cuts at: 4690 4750 4888 4690
Size: 60 138 7498
Fragments arranged by size:
7498 138 60
KpnI G_GTAC'C
Cuts at: 5021 5021
Size: 7696
MfeI C'AATT_G
Cuts at: 5295 5295
Size: 7696
MluI A'CGCG_T
Cuts at: 7572 7572
Size: 7696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 3522 6810 6994 1305
Size: 1632 585 3288 184 2007
Fragments arranged by size:
3288 2007 1632 585 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3831 10
Size: 54 1425 343 1999 3875
Fragments arranged by size:
3875 1999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 2663 3654 4184 5654 6013 6172
Size: 648 1311 186 991 530 1470 359 159
Cuts at: 6172 518
Size: 2042
Fragments arranged by size:
2042 1470 1311 991 648 530 359 186
159
MspA1I CmG'CkG
Cuts at: 1606 2036 2063 2328 2620 2863 3457 3514 3784
Size: 430 27 265 292 243 594 57 270
Cuts at: 3784 3816 4116 4249 4467 4677 4831 5112 5789
Size: 32 300 133 218 210 154 281 677
Cuts at: 5789 6937 7182 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 594 430 300 292 281
270 265 245 243 218 210 154 133
57 32 27
NaeI GCC'GGC
Cuts at: 2114 2755 3014 4414 4598 2114
Size: 641 259 1400 184 5212
Fragments arranged by size:
5212 1400 641 259 184
NarI GG'CG_CC
Cuts at: 4691 4751 4889 4691
Size: 60 138 7498
Fragments arranged by size:
7498 138 60
NcoI C'CATG_G
Cuts at: 513 1065 2022 3186 513
Size: 552 957 1164 5023
Fragments arranged by size:
5023 1164 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7696
NgoMIV G'CCGG_C
Cuts at: 2112 2753 3012 4412 4596 2112
Size: 641 259 1400 184 5212
Fragments arranged by size:
5212 1400 641 259 184
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7696
NotI GC'GGCC_GC
Cuts at: 5143 5143
Size: 7696
NspI r_CATG'y
Cuts at: 1132 1229 2929 5054 6599 1132
Size: 97 1700 2125 1545 2229
Fragments arranged by size:
2229 2125 1700 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3398 4591 1348
Size: 2050 1193 4453
Fragments arranged by size:
4453 2050 1193
PciI A'CATG_T
Cuts at: 6595 6595
Size: 7696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2527 2566 2781 4591 4768 1108
Size: 1419 39 215 1810 177 4036
Fragments arranged by size:
4036 1810 1419 215 177 39
PfoI T'CCnGG_A
Cuts at: 2416 2805 3294 3630 4767 2416
Size: 389 489 336 1137 5345
Fragments arranged by size:
5345 1137 489 389 336
PmeI GTTT'AAAC
Cuts at: 5000 5000
Size: 7696
PmlI CAC'GTG
Cuts at: 2524 3379 2524
Size: 855 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2171 2203 3611 3643 3923 3955 5765 5797 7278
Size: 32 1408 32 280 32 1810 32 1481
Cuts at: 7278 7310 2171
Size: 32 2557
Fragments arranged by size:
2557 1810 1481 1408 280 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2066 3599 4318 2066
Size: 1533 719 5444
Fragments arranged by size:
5444 1533 719
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7696
PsiI TTA'TAA
Cuts at: 5266 5266
Size: 7696
PspOMI G'GGCC_C
Cuts at: 3036 3459 4702 3036
Size: 423 1243 6030
Fragments arranged by size:
6030 1243 423
PstI C_TGCA'G
Cuts at: 830 2625 2958 3180 3287 4050 4248 5048 830
Size: 1795 333 222 107 763 198 800 3478
Fragments arranged by size:
3478 1795 800 763 333 222 198 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7696
PvuII CAG'CTG
Cuts at: 2620 2863 3514 4116 4249 2620
Size: 243 651 602 133 6067
Fragments arranged by size:
6067 651 602 243 133
SacI G_AGCT'C
Cuts at: 4275 4608 4275
Size: 333 7363
SacII CC_GC'GG
Cuts at: 2037 2064 2329 4468 4832 2037
Size: 27 265 2139 364 4901
Fragments arranged by size:
4901 2139 364 265 27
SalI G'TCGA_C
Cuts at: 1936 5038 1936
Size: 3102 4594
SanDI GG'GwC_CC
Cuts at: 2066 3599 2066
Size: 1533 6163
SbfI CC_TGCA'GG
Cuts at: 2958 5048 2958
Size: 2090 5606
ScaI AGT'ACT
Cuts at: 5944 7656 5944
Size: 1712 5984
SexAI A'CCwGG_T
Cuts at: 2562 2562
Size: 7696
SfcI C'TryA_G
Cuts at: 826 1045 2621 2954 3176 3283 4046 4244 5044
Size: 219 1576 333 222 107 763 198 800
Cuts at: 5044 6179 6860 7051 826
Size: 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 800 763 681 333 222
219 198 191 107
SfoI GGC'GCC
Cuts at: 4692 4752 4890 4692
Size: 60 138 7498
Fragments arranged by size:
7498 138 60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7696
SgrAI Cr'CCGG_yG
Cuts at: 1700 2753 1700
Size: 1053 6643
SmaI CCC'GGG
Cuts at: 1872 2195 2312 3391 4871 5023 1872
Size: 323 117 1079 1480 152 4545
Fragments arranged by size:
4545 1480 1079 323 152 117
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5800 6701 6963 820
Size: 197 926 3857 901 262 1553
Fragments arranged by size:
3857 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4993 5487 493
Size: 4500 494 2702
Fragments arranged by size:
4500 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7696
SphI G_CATG'C
Cuts at: 1132 2929 5054 1132
Size: 1797 2125 3774
Fragments arranged by size:
3774 2125 1797
SspI AAT'ATT
Cuts at: 5 52 5620 5
Size: 47 5568 2081
Fragments arranged by size:
5568 2081 47
StuI AGG'CCT
Cuts at: 1517 3302 1517
Size: 1785 5911
StyI C'CwwG_G
Cuts at: 513 1065 2022 3186 3826 3882 5135 513
Size: 552 957 1164 640 56 1253 3074
Fragments arranged by size:
3074 1253 1164 957 640 552 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2957 3238 4727 5723 5740 5893 6078
Size: 1255 1377 281 1489 996 17 153 185
Cuts at: 6078 6523 325
Size: 445 1498
Fragments arranged by size:
1498 1489 1377 1255 996 445 281 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2265 5942 7654
Size: 274 80 33 51 1443 288 3677 1712
Cuts at: 7654 96
Size: 138
Fragments arranged by size:
3677 1712 1443 288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7656 7688 7656
Size: 32 7664
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7696
XbaI T'CTAG_A
Cuts at: 5032 5032
Size: 7696
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7696
XmaI C'CCGG_G
Cuts at: 1870 2193 2310 3389 4869 5021 1870
Size: 323 117 1079 1480 152 4545
Fragments arranged by size:
4545 1480 1079 323 152 117
XmnI GAAnn'nnTTC
Cuts at: 5825 5825
Size: 7696
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3130 5504 276
Size: 53 83 186 936 1596 2374 2468
Fragments arranged by size:
2468 2374 1596 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI
FspAI NruI NsiI PacI PspXI PsrI RsrII SapI
SfiI SrfI SwaI XcmI