Restriction map for
pFN21ASDF0041
(Circular) (Six-base) MAPSORT of: pFN21ASDF0041.seq Check: 7850 from: 1 to: 5662
pFN21ASDF0041 5662 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 2325 2512 2779 2189
Size: 136 187 267 5072
Fragments arranged by size:
5072 267 187 136
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2904 3472 278
Size: 53 83 186 936 1368 568 2468
Fragments arranged by size:
2468 1368 936 568 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 3005 1937
Size: 730 338 4594
Fragments arranged by size:
4594 730 338
Acc65I G'GTAC_C
Cuts at: 2238 2983 2238
Size: 745 4917
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3725 5109 5377 1632
Size: 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2710 3405 4977 2710
Size: 695 1572 3395
Fragments arranged by size:
3395 1572 695
ApaI G_GGCC'C
Cuts at: 2198 2198
Size: 5662
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2971 3197 3349 5458 1493
Size: 1478 226 152 2109 1697
Fragments arranged by size:
2109 1697 1478 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2156 2248 2786 2987 1870
Size: 73 213 92 538 201 4545
Fragments arranged by size:
4545 538 213 201 92 73
AvrII C'CTAG_G
Cuts at: 2101 2101
Size: 5662
BamHI G'GATC_C
Cuts at: 2066 2992 3359 2066
Size: 926 367 4369
Fragments arranged by size:
4369 926 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 2478 2507 2618 2802
Size: 325 217 627 451 240 29 111 184
Cuts at: 2802 2983 4438 618
Size: 181 1455 1842
Fragments arranged by size:
1842 1455 627 451 325 240 217 184
181 111 29
BanII G_rGCy'C
Cuts at: 1391 2198 2868 2981 1391
Size: 807 670 113 4072
Fragments arranged by size:
4072 807 670 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2896 928
Size: 971 997 3694
Fragments arranged by size:
3694 997 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2775 3554 4764 2775
Size: 779 1210 3673
Fragments arranged by size:
3673 1210 779
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 4273 136
Size: 107 122 71 631 605 373 2228 1525
Fragments arranged by size:
2228 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 5657 5657
Size: 5662
BlpI GC'TnA_GC
Cuts at: 2364 3079 2364
Size: 715 4947
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2214 2704 3723 4879 1790
Size: 408 16 490 1019 1156 2573
Fragments arranged by size:
2573 1156 1019 490 408 16
BmgBI CAC'GTC
Cuts at: 1318 2235 1318
Size: 917 4745
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2399 2871 4322 1239
Size: 824 336 472 1451 2579
Fragments arranged by size:
2579 1451 824 472 336
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4325 882
Size: 617 2826 2219
Fragments arranged by size:
2826 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2959 3453 493
Size: 711 1755 494 2702
Fragments arranged by size:
2702 1755 711 494
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2901 3469 3851
Size: 53 83 186 936 495 873 568 382
Cuts at: 3851 4486 275
Size: 635 1451
Fragments arranged by size:
1451 936 873 635 568 495 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2107 4095 4493 4767 4914 5325 2107
Size: 1988 398 274 147 411 2444
Fragments arranged by size:
2444 1988 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 1178
Size: 30 165 30 1152 30 4255
Fragments arranged by size:
4255 1152 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2563 2874 1186
Size: 1377 311 3974
Fragments arranged by size:
3974 1377 311
BseYI C'CCAG_C
Cuts at: 2061 2823 2867 4865 5361 2061
Size: 762 44 1998 496 2362
Fragments arranged by size:
2362 1998 762 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2303 2546 1163
Size: 573 546 21 243 4279
Fragments arranged by size:
4279 573 546 243 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2496 3723 3808 4879 2496
Size: 1227 85 1071 3279
Fragments arranged by size:
3279 1227 1071 85
BsmI GAATG_Cn'
Cuts at: 3173 3266 3173
Size: 93 5569
BsmBI CGTCTCn'nnnn_
Cuts at: 2892 2892
Size: 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2214 2496 2704 2868 2981 3723
Size: 399 408 16 282 208 164 113 742
Cuts at: 3723 3808 4879 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 282 208 164
113 85 16
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 2276 2325 2512 2779 3017 844
Size: 1345 87 49 187 267 238 3489
Fragments arranged by size:
3489 1345 267 238 187 87 49
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2280 4306 4493 1700
Size: 110 238 232 2026 187 2869
Fragments arranged by size:
2869 2026 238 232 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 2007 4466 2007
Size: 2459 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2557 2041
Size: 516 5146
BstYI r'GATC_y
Cuts at: 1972 2066 2992 3359 3744 3761 5213 5299 5311
Size: 94 926 367 385 17 1452 86 12
Cuts at: 5311 5657 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 926 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2034 2323 2371 2398 2503 2511
Size: 552 957 12 289 48 27 105 8
Cuts at: 2511 513
Size: 3664
Fragments arranged by size:
3664 957 552 289 105 48 27 12
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 3178 3972 3992 3178
Size: 794 20 4848
Fragments arranged by size:
4848 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 2218 2218
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3109 3998 5627
Size: 54 1425 50 275 18 1279 889 1629
Cuts at: 5627 8
Size: 43
Fragments arranged by size:
1629 1425 1279 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2090 3592 1353
Size: 728 9 1502 3423
Fragments arranged by size:
3423 1502 728 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2399 2871 3725 4322 5109 5377
Size: 393 431 336 472 854 597 787 268
Cuts at: 5377 1239
Size: 1524
Fragments arranged by size:
1524 854 787 597 472 431 393 336
268
EcoO109I rG'GnC_Cy
Cuts at: 2195 2308 2891 2195
Size: 113 583 4966
Fragments arranged by size:
4966 583 113
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 2423 4168 2423
Size: 1745 3917
HaeII r_GCGC'y
Cuts at: 1678 2062 2281 2371 4809 1678
Size: 384 219 90 2438 2531
Fragments arranged by size:
2531 2438 384 219 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2531 2563 4303
Size: 32 135 32 486 32 441 32 1740
Cuts at: 4303 4335 4377 4409 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1740 486 441 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3006 3252 669
Size: 988 281 1068 246 3079
Fragments arranged by size:
3079 1068 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5662
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2242 2987 2242
Size: 745 4917
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2415 2574 2715 4776 4960 1305
Size: 1110 159 141 2061 184 2007
Fragments arranged by size:
2061 2007 1110 184 159 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3840
Fragments arranged by size:
3840 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3620 3979 4138 518
Size: 648 1251 1203 359 159 2042
Fragments arranged by size:
2042 1251 1203 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2055 2827 3078 3755 4903 5148 1606
Size: 449 772 251 677 1148 245 2120
Fragments arranged by size:
2120 1148 772 677 449 251 245
NaeI GCC'GGC
Cuts at: 2050 2282 2050
Size: 232 5430
NcoI C'CATG_G
Cuts at: 513 1065 2022 2323 2398 2503 2511 513
Size: 552 957 301 75 105 8 3664
Fragments arranged by size:
3664 957 552 301 105 75 8
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NgoMIV G'CCGG_C
Cuts at: 2048 2280 2048
Size: 232 5430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2776 1348
Size: 1428 4234
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2359 2377 2710 1108
Size: 1251 18 333 4060
Fragments arranged by size:
4060 1251 333 18
PfoI T'CCnGG_A
Cuts at: 2304 2304
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2308
Size: 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PspOMI G'GGCC_C
Cuts at: 2194 2194
Size: 5662
PspXI vC'TCGA_Gb
Cuts at: 2248 2248
Size: 5662
PstI C_TGCA'G
Cuts at: 830 2186 2523 2605 3014 830
Size: 1356 337 82 409 3478
Fragments arranged by size:
3478 1356 409 337 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
PvuII CAG'CTG
Cuts at: 2055 2055
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SapI GCTCTTCn'nnn_
Cuts at: 2081 2081
Size: 5662
SbfI CC_TGCA'GG
Cuts at: 2186 3014 2186
Size: 828 4834
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 2182 2519 2601 3010 4145 4826 5017
Size: 219 1137 337 82 409 1135 681 191
Cuts at: 5017 826
Size: 1471
Fragments arranged by size:
1471 1137 1135 681 409 337 219 191
82
SfiI GGCCn_nnn'nGGCC
Cuts at: 2045 2045
Size: 5662
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2248 3766 4667 4929 820
Size: 197 926 305 1518 901 262 1553
Fragments arranged by size:
1553 1518 926 901 305 262 197
SnaBI TAC'GTA
Cuts at: 493 2959 3453 493
Size: 2466 494 2702
Fragments arranged by size:
2702 2466 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2022 2101 2323 2398 2503 2511 3101
Size: 552 957 79 222 75 105 8 590
Cuts at: 3101 513
Size: 3074
Fragments arranged by size:
3074 957 590 552 222 105 79 75
8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3689 3706 3859 4044 4489 325
Size: 1255 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2795 2827 5622 5654 2795
Size: 32 2795 32 2803
Fragments arranged by size:
2803 2795 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2924 1316
Size: 1608 4054
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2356
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 2248 1943
Size: 305 5357
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2902 3470 276
Size: 53 83 186 936 1368 568 2468
Fragments arranged by size:
2468 1368 936 568 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PsrI
RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI