Restriction map for
pFN21ASDB2508
(Circular) (Six-base) MAPSORT of: pFN21ASDB2508.seq Check: 1332 from: 1 to: 5443
pFN21ASDB2508 5443 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 3506 4890 5158 1632
Size: 779 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 779 268
AfeI AGC'GCT
Cuts at: 2086 2086
Size: 5443
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 2232 4342 5319 1203
Size: 1029 2110 977 1327
Fragments arranged by size:
2110 1327 1029 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2105 1184
Size: 921 4522
AlwNI CAG_nnn'CTG
Cuts at: 2218 2570 2644 3186 4758 2218
Size: 352 74 542 1572 2903
Fragments arranged by size:
2903 1572 542 352 74
ApaLI G'TGCA_C
Cuts at: 2155 2246 3500 4656 2155
Size: 91 1254 1156 2942
Fragments arranged by size:
2942 1254 1156 91
ApoI r'AATT_y
Cuts at: 1493 2752 2978 3130 5239 1493
Size: 1259 226 152 2109 1697
Fragments arranged by size:
2109 1697 1259 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2768 1870
Size: 73 131 694 4545
Fragments arranged by size:
4545 694 131 73
BamHI G'GATC_C
Cuts at: 2519 2773 3140 2519
Size: 254 367 4822
Fragments arranged by size:
4822 367 254
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2132 2198 2764 4219
Size: 325 217 627 271 74 66 566 1455
Cuts at: 4219 618
Size: 1842
Fragments arranged by size:
1842 1455 627 566 325 271 217 74
66
BanII G_rGCy'C
Cuts at: 1391 2075 2175 2679 2762 1391
Size: 684 100 504 83 4072
Fragments arranged by size:
4072 684 504 100 83
BbeI G_GCGC'C
Cuts at: 2062 2136 2062
Size: 74 5369
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2146 2180 3634 3668 1903
Size: 34 209 34 1454 34 3678
Fragments arranged by size:
3678 1454 209 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2056 2079 4054
Size: 107 122 71 631 605 384 23 1975
Cuts at: 4054 136
Size: 1525
Fragments arranged by size:
1975 1525 631 605 384 122 107 71
23
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2250 3504 4660 1790
Size: 369 91 1254 1156 2573
Fragments arranged by size:
2573 1254 1156 369 91
BmgBI CAC'GTC
Cuts at: 1318 2233 1318
Size: 915 4528
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3174 4132 442
Size: 416 53 83 399 24 1757 958 1753
Fragments arranged by size:
1757 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2666 4103 1239
Size: 1427 1437 2579
Fragments arranged by size:
2579 1437 1427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3568 4433 4731 3568
Size: 865 298 4280
Fragments arranged by size:
4280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2230 4106 882
Size: 617 731 1876 2219
Fragments arranged by size:
2219 1876 731 617
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3234 493
Size: 711 1536 494 2702
Fragments arranged by size:
2702 1536 711 494
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2059 2133 3250 3632
Size: 53 83 186 936 526 74 1117 382
Cuts at: 3632 4267 275
Size: 635 1451
Fragments arranged by size:
1451 1117 936 635 526 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 3876 4274 4548 4695 5106 3876
Size: 398 274 147 411 4213
Fragments arranged by size:
4213 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2648 1186
Size: 897 565 3981
Fragments arranged by size:
3981 897 565
BseYI C'CCAG_C
Cuts at: 2218 2490 4646 5142 2218
Size: 272 2156 496 2519
Fragments arranged by size:
2519 2156 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4870
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2159 2250 3504 3589 4660 2159
Size: 91 1254 85 1071 2942
Fragments arranged by size:
2942 1254 1071 91 85
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2159 2175 2250 2679 2762 3504
Size: 399 285 84 16 75 429 83 742
Cuts at: 3504 3589 4660 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 285 85 84
83 75 16
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2798 844
Size: 1421 533 3489
Fragments arranged by size:
3489 1421 533
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 4087 4274 1700
Size: 110 251 2026 187 2869
Fragments arranged by size:
2869 2026 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 2007 4247 2007
Size: 2240 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2519 2773 3140 3525 3542 4994 5080 5092
Size: 547 254 367 385 17 1452 86 12
Cuts at: 5092 5438 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 547 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2191 2515 2592 513
Size: 552 1126 324 77 3364
Fragments arranged by size:
3364 1126 552 324 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2293 487
Size: 683 56 1067 3637
Fragments arranged by size:
3637 1067 683 56
BtsI GCAGTG_nn'
Cuts at: 2157 2444 2959 3753 3773 2157
Size: 287 515 794 20 3827
Fragments arranged by size:
3827 794 515 287 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2434 2469 529
Size: 35 1870 35 3503
Fragments arranged by size:
3503 1870 35 35
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4450 809
Size: 507 3134 1802
Fragments arranged by size:
3134 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 2890 3779
Size: 54 1425 50 275 18 759 301 889
Cuts at: 3779 5408 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 759 301 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2625 2661 3373 1353
Size: 1272 36 712 3423
Fragments arranged by size:
3423 1272 712 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2080 2702 4030 4404 4550 387
Size: 1064 138 491 622 1328 374 146 1280
Fragments arranged by size:
1328 1280 1064 622 491 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2666 3506 4103 4890 5158 1239
Size: 393 779 255 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 779 597 393 268 255
EcoRI G'AATT_C
Cuts at: 2752 3130 2752
Size: 378 5065
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 2062 2088 2136 2153 2645 4590 1678
Size: 384 26 48 17 492 1945 2531
Fragments arranged by size:
2531 1945 492 384 48 26 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2386 2418 2511 2543 2568
Size: 32 135 32 814 32 93 32 25
Cuts at: 2568 2600 4084 4116 4158 4190 1373
Size: 32 1484 32 42 32 2626
Fragments arranged by size:
2626 1484 814 135 93 42 32 32
32 32 32 32 32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KasI G'GCGC_C
Cuts at: 2058 2132 2058
Size: 74 5369
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 3042 3042
Size: 5443
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 4557 4741 1305
Size: 948 2304 184 2007
Fragments arranged by size:
2304 2007 948 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 2862
Fragments arranged by size:
2862 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 3536 4684 4929 1606
Size: 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 677 245
NaeI GCC'GGC
Cuts at: 2063 2063
Size: 5443
NarI GG'CG_CC
Cuts at: 2059 2133 2059
Size: 74 5369
NcoI C'CATG_G
Cuts at: 513 1065 2515 513
Size: 552 1450 3441
Fragments arranged by size:
3441 1450 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5443
NgoMIV G'CCGG_C
Cuts at: 2061 2061
Size: 5443
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2801 4346 1132
Size: 97 1572 1545 2229
Fragments arranged by size:
2229 1572 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5443
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2218 1108
Size: 1110 4333
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 5411
PstI C_TGCA'G
Cuts at: 830 2439 2795 830
Size: 1609 356 3478
Fragments arranged by size:
3478 1609 356
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SexAI A'CCwGG_T
Cuts at: 2556 2556
Size: 5443
SfcI C'TryA_G
Cuts at: 826 1045 2435 2791 3926 4607 4798 826
Size: 219 1390 356 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 681 356 219 191
SfoI GGC'GCC
Cuts at: 2060 2134 2060
Size: 74 5369
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 2061 1700
Size: 361 5082
SmaI CCC'GGG
Cuts at: 1872 2076 2770 1872
Size: 204 694 4545
Fragments arranged by size:
4545 694 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3547 4448 4710 820
Size: 197 926 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2740 3234 493
Size: 2247 494 2702
Fragments arranged by size:
2702 2247 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2801 1132
Size: 1669 3774
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2515 2882 513
Size: 552 1450 367 3074
Fragments arranged by size:
3074 1450 552 367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5403 5435 5403
Size: 32 5411
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2074 2768 1870
Size: 204 694 4545
Fragments arranged by size:
4545 694 204
XmnI GAAnn'nnTTC
Cuts at: 2267 3572 2267
Size: 1305 4138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI XcmI