Restriction map for
pFN21ASDB1467
(Circular) (Six-base) MAPSORT of: pFN21ASDB1467.seq Check: 2489 from: 1 to: 9631
pFN21ASDB1467 9631 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3212 4096 3212
Size: 884 8747
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7441 278
Size: 53 83 186 936 5905 2468
Fragments arranged by size:
5905 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3225 6974 1937
Size: 280 1008 3749 4594
Fragments arranged by size:
4594 3749 1008 280
Acc65I G'GTAC_C
Cuts at: 6952 6952
Size: 9631
AclI AA'CG_TT
Cuts at: 1563 7758 8131 1563
Size: 6195 373 3063
Fragments arranged by size:
6195 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2800 3176 3623 4233 4234 4667 4820 4916
Size: 1168 376 447 610 1 433 153 96
Cuts at: 4916 5651 7694 9078 9346 1632
Size: 735 2043 1384 268 1917
Fragments arranged by size:
2043 1917 1384 1168 735 610 447 433
376 268 153 96 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9434
AflIII A'CryG_T
Cuts at: 1203 5646 8530 9507 1203
Size: 4443 2884 977 1327
Fragments arranged by size:
4443 2884 1327 977
AgeI A'CCGG_T
Cuts at: 8462 8462
Size: 9631
AhdI GACnn_n'nnGTC
Cuts at: 5640 8360 5640
Size: 2720 6911
AleI CACnn'nnGTG
Cuts at: 1166 4135 1166
Size: 2969 6662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2876 4043 5127 1184
Size: 1692 1167 1084 5688
Fragments arranged by size:
5688 1692 1167 1084
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4904 4936 4904
Size: 32 9599
AlwNI CAG_nnn'CTG
Cuts at: 2787 3247 3532 4261 4760 4798 4942 5181 7374
Size: 460 285 729 499 38 144 239 2193
Cuts at: 7374 8946 2787
Size: 1572 3472
Fragments arranged by size:
3472 2193 1572 729 499 460 285 239
144 38
ApaI G_GGCC'C
Cuts at: 6517 6517
Size: 9631
ApaLI G'TGCA_C
Cuts at: 4329 7688 8844 4329
Size: 3359 1156 5116
Fragments arranged by size:
5116 3359 1156
ApoI r'AATT_y
Cuts at: 1493 3726 6651 6940 7166 7318 9427 1493
Size: 2233 2925 289 226 152 2109 1697
Fragments arranged by size:
2925 2233 2109 1697 289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8185 160
Size: 872 7153 1606
Fragments arranged by size:
7153 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9631
AvaI C'yCGr_G
Cuts at: 1870 1943 2505 3137 3860 4122 6333 6456 6956
Size: 73 562 632 723 262 2211 123 500
Cuts at: 6956 1870
Size: 4545
Fragments arranged by size:
4545 2211 723 632 562 500 262 123
73
BamHI G'GATC_C
Cuts at: 2640 3736 5236 5880 6753 6961 7328 2640
Size: 1096 1500 644 873 208 367 4943
Fragments arranged by size:
4943 1500 1096 873 644 367 208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2682 2872 4207 6476 6917
Size: 325 217 627 895 190 1335 2269 441
Cuts at: 6917 6952 8407 618
Size: 35 1455 1842
Fragments arranged by size:
2269 1842 1455 1335 895 627 441 325
217 190 35
BanII G_rGCy'C
Cuts at: 1391 2471 2963 3464 4045 4181 4577 4689 6059
Size: 1080 492 501 581 136 396 112 1370
Cuts at: 6059 6113 6517 6786 6950 1391
Size: 54 404 269 164 4072
Fragments arranged by size:
4072 1370 1080 581 501 492 404 396
269 164 136 112 54
BbeI G_GCGC'C
Cuts at: 2686 2686
Size: 9631
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 3530 4470 5639 5719 6887 928
Size: 971 120 1511 940 1169 80 1168 3672
Fragments arranged by size:
3672 1511 1169 1168 971 940 120 80
BbvCI CC'TCA_GC
Cuts at: 3995 3995
Size: 9631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4539 4573 5297 5331 7822 7856 1903
Size: 34 2602 34 724 34 2491 34 3678
Fragments arranged by size:
3678 2602 2491 724 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2515 5935 7523 8733 2515
Size: 3420 1588 1210 3413
Fragments arranged by size:
3420 3413 1588 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9631
BfrBI ATG'CAT
Cuts at: 6595 6595
Size: 9631
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3310 4005 5030
Size: 107 122 71 631 605 1638 695 1025
Cuts at: 5030 8242 136
Size: 3212 1525
Fragments arranged by size:
3212 1638 1525 1025 695 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2568 4089 5331 6153 9626 2568
Size: 1521 1242 822 3473 2573
Fragments arranged by size:
3473 2573 1521 1242 822
BlpI GC'TnA_GC
Cuts at: 4761 6791 7048 4761
Size: 2030 257 7344
Fragments arranged by size:
7344 2030 257
Bme1580I G_kGCm'C
Cuts at: 1790 2877 3861 4333 5175 5719 6479 6517 7692
Size: 1087 984 472 842 544 760 38 1175
Cuts at: 7692 8848 1790
Size: 1156 2573
Fragments arranged by size:
2573 1175 1156 1087 984 842 760 544
472 38
BmgBI CAC'GTC
Cuts at: 1318 3283 1318
Size: 1965 7666
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2574 2699 3735
Size: 416 53 83 399 24 1157 125 1036
Cuts at: 3735 4094 5142 7362 8320 442
Size: 359 1048 2220 958 1753
Fragments arranged by size:
2220 1753 1157 1048 1036 958 416 399
359 125 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2096 1056
Size: 1040 8591
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2420 4591 4890 2420
Size: 2171 299 7161
Fragments arranged by size:
7161 2171 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2415 3127 3335 4437 5111 5921 6602
Size: 845 331 712 208 1102 674 810 681
Cuts at: 6602 6770 8291 1239
Size: 168 1521 2579
Fragments arranged by size:
2579 1521 1102 845 810 712 681 674
331 208 168
Bpu10I CC'TnA_GC
Cuts at: 3995 6873 3995
Size: 2878 6753
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4885 4892 5894 6089 7756 8621 8919 2240
Size: 2645 7 1002 195 1667 865 298 2952
Fragments arranged by size:
2952 2645 1667 1002 865 298 195 7
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2276 3010 5423 8294 882
Size: 617 777 734 2413 2871 2219
Fragments arranged by size:
2871 2413 2219 777 734 617
BsaAI yAC'GTr
Cuts at: 493 1204 5586 6928 7422 493
Size: 711 4382 1342 494 2702
Fragments arranged by size:
4382 2702 1342 711 494
BsaBI GATnn'nnATC
Cuts at: 5318 9625 5318
Size: 4307 5324
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2683 2921 7438 7820
Size: 53 83 186 936 1150 238 4517 382
Cuts at: 7820 8455 275
Size: 635 1451
Fragments arranged by size:
4517 1451 1150 936 635 382 238 186
83 53
BsaWI w'CCGG_w
Cuts at: 8064 8462 8736 8883 9294 8064
Size: 398 274 147 411 8401
Fragments arranged by size:
8401 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2357 3200 3230 5180
Size: 30 165 30 924 30 843 30 1950
Cuts at: 5180 5210 5513 5543 6330 6360 6528 6558 1178
Size: 30 303 30 787 30 168 30 4251
Fragments arranged by size:
4251 1950 924 843 787 303 168 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2484 3038 3332 4171 5108 6257 6380
Size: 985 313 554 294 839 937 1149 123
Cuts at: 6380 6821 1186
Size: 441 3996
Fragments arranged by size:
3996 1149 985 937 839 554 441 313
294 123
BseYI C'CCAG_C
Cuts at: 2199 2574 2662 4224 4313 4604 4608 4782 5034
Size: 375 88 1562 89 291 4 174 252
Cuts at: 5034 6299 6484 8834 9330 2199
Size: 1265 185 2350 496 2500
Fragments arranged by size:
2500 2350 1562 1265 496 375 291 252
185 174 89 88 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4039 4124 4754 1163
Size: 573 2303 85 630 6040
Fragments arranged by size:
6040 2303 630 573 85
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5392 6328 7081 7842 8870 1540
Size: 275 186 3391 936 753 761 1028 2301
Fragments arranged by size:
3391 2301 1028 936 761 753 275 186
BsiHKAI G_wGCw'C
Cuts at: 3135 3685 4045 4181 4333 4577 4593 6113 6786
Size: 550 360 136 152 244 16 1520 673
Cuts at: 6786 7692 7777 8848 3135
Size: 906 85 1071 3918
Fragments arranged by size:
3918 1520 1071 906 673 550 360 244
152 136 85 16
BsiWI C'GTAC_G
Cuts at: 5586 5586
Size: 9631
BsmI GAATG_Cn'
Cuts at: 2920 4814 7142 7235 2920
Size: 1894 2328 93 5316
Fragments arranged by size:
5316 2328 1894 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2471 2877 2963 3135 3464 3685 3861
Size: 399 681 406 86 172 329 221 176
Cuts at: 3861 4045 4181 4333 4577 4593 4689 5175 5719
Size: 184 136 152 244 16 96 486 544
Cuts at: 5719 6059 6113 6479 6517 6786 6950 7692 7777
Size: 340 54 366 38 269 164 742 85
Cuts at: 7777 8848 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 681 544 486 406 399
366 340 329 269 244 221 184 176
172 164 152 136 96 86 85 54
38 16
BspEI T'CCGG_A
Cuts at: 9294 9294
Size: 9631
BspHI T'CATG_A
Cuts at: 3352 5322 7520 9250 3352
Size: 1970 2198 1730 3733
Fragments arranged by size:
3733 2198 1970 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3212 4096 4246 4785 5029 6722 6777 6986
Size: 2368 884 150 539 244 1693 55 209
Cuts at: 6986 844
Size: 3489
Fragments arranged by size:
3489 2368 1693 884 539 244 209 150
55
BsrBI CCG'CTC
Cuts at: 1394 1823 6330 6355 7518 1394
Size: 429 4507 25 1163 3507
Fragments arranged by size:
4507 3507 1163 429 25
BsrDI GCAATG_nn'
Cuts at: 65 1248 2047 8126 8300 65
Size: 1183 799 6079 174 1396
Fragments arranged by size:
6079 1396 1183 799 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4113 5387 5465 5481 6066 6884 8275
Size: 110 2303 1274 78 16 585 818 1391
Cuts at: 8275 8462 1700
Size: 187 2869
Fragments arranged by size:
2869 2303 1391 1274 818 585 187 110
78 16
BsrGI T'GTAC_A
Cuts at: 96 6432 96
Size: 6336 3295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9631
BssSI C'ACGA_G
Cuts at: 5125 6212 7691 8703 5125
Size: 1087 1479 1012 6053
Fragments arranged by size:
6053 1479 1087 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 9631
BstBI TT'CG_AA
Cuts at: 2007 8435 2007
Size: 6428 3203
BstEII G'GTnAC_C
Cuts at: 1184 5461 6764 1184
Size: 4277 1303 4051
Fragments arranged by size:
4277 4051 1303
BstXI CCAn_nnnn'nTGG
Cuts at: 3748 3913 5155 5693 3748
Size: 165 1242 538 7686
Fragments arranged by size:
7686 1242 538 165
BstYI r'GATC_y
Cuts at: 1972 2368 2568 2640 3736 4089 5236 5331 5442
Size: 396 200 72 1096 353 1147 95 111
Cuts at: 5442 5880 6153 6753 6902 6961 7328 7713 7730
Size: 438 273 600 149 59 367 385 17
Cuts at: 7730 9182 9268 9280 9626 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1147 1096 600 438 396 385
367 353 346 273 200 149 111 95
86 72 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2868 3269 5930 5937 6408 2868
Size: 401 2661 7 471 6091
Fragments arranged by size:
6091 2661 471 401 7
BtgI C'CryG_G
Cuts at: 513 1065 2841 3657 4000 513
Size: 552 1776 816 343 6144
Fragments arranged by size:
6144 1776 816 552 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2111 487
Size: 683 56 885 8007
Fragments arranged by size:
8007 885 683 56
BtsI GCAGTG_nn'
Cuts at: 3433 3861 4130 4658 4742 6004 6449 7147 7941
Size: 428 269 528 84 1262 445 698 794
Cuts at: 7941 7961 3433
Size: 20 5103
Fragments arranged by size:
5103 1262 794 698 528 445 428 269
84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9631
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9596
DraI TTT'AAA
Cuts at: 6935 6935
Size: 9631
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3617 8638 809
Size: 507 2301 5021 1802
Fragments arranged by size:
5021 2301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2844 2986
Size: 54 1425 50 275 18 270 744 142
Cuts at: 2986 3651 4270 4292 5022 5389 5580 5665 6115
Size: 665 619 22 730 367 191 85 450
Cuts at: 6115 6325 6348 6414 7078 7967 9596 8
Size: 210 23 66 664 889 1629 43
Fragments arranged by size:
1629 1425 889 744 730 665 664 619
450 367 275 270 210 191 142 85
66 54 50 43 23 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 5389 6325 7078 1537
Size: 275 3577 936 753 4090
Fragments arranged by size:
4090 3577 936 753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2443 2643 2649 3006 4033 4067 4247 4980
Size: 1090 200 6 357 1027 34 180 733
Cuts at: 4980 5849 6284 6584 7561 1353
Size: 869 435 300 977 3423
Fragments arranged by size:
3423 1090 1027 977 869 733 435 357
300 200 180 34 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2260 6373 6839 8218 8592 8738
Size: 1064 138 671 4113 466 1379 374 146
Cuts at: 8738 387
Size: 1280
Fragments arranged by size:
4113 1379 1280 1064 671 466 374 146
138
EcoICRI GAG'CTC
Cuts at: 4043 4179 4575 6111 6784 4043
Size: 136 396 1536 673 6890
Fragments arranged by size:
6890 1536 673 396 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2415 2800 3127 3176 3335 3623
Size: 393 452 331 385 327 49 159 288
Cuts at: 3623 4233 4234 4437 4667 4820 4916 5111 5651
Size: 610 1 203 230 153 96 195 540
Cuts at: 5651 5921 6602 6770 7694 8291 9078 9346 1239
Size: 270 681 168 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 681 610 597 540 452
393 385 331 327 288 270 268 230
203 195 168 159 153 96 49 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2648 3884 5093 5288 6836 6872 2648
Size: 1236 1209 195 1548 36 5407
Fragments arranged by size:
5407 1548 1236 1209 195 36
EcoO109I rG'GnC_Cy
Cuts at: 2550 3529 3909 4258 4795 5905 2550
Size: 979 380 349 537 1110 6276
Fragments arranged by size:
6276 1110 979 537 380 349
EcoRI G'AATT_C
Cuts at: 6940 7318 6940
Size: 378 9253
EcoRV GAT'ATC
Cuts at: 3055 3055
Size: 9631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3358 4078 5125 3358
Size: 720 1047 7864
Fragments arranged by size:
7864 1047 720
FspI TGC'GCA
Cuts at: 3425 5360 8137 3425
Size: 1935 2777 4919
Fragments arranged by size:
4919 2777 1935
FspAI rTGC'GCAy
Cuts at: 5360 5360
Size: 9631
HaeII r_GCGC'y
Cuts at: 1678 2686 4320 4537 4602 8778 1678
Size: 1008 1634 217 65 4176 2531
Fragments arranged by size:
4176 2531 1634 1008 217 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2499 2531 3759
Size: 32 135 32 545 32 350 32 1228
Cuts at: 3759 3791 5626 5658 8272 8304 8346 8378 1373
Size: 32 1835 32 2614 32 42 32 2626
Fragments arranged by size:
2626 2614 1835 1228 545 350 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3151 5797 6975 7221 669
Size: 988 281 1213 2646 1178 246 3079
Fragments arranged by size:
3079 2646 1213 1178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4277 4475 748
Size: 3529 198 5904
Fragments arranged by size:
5904 3529 198
HpaI GTT'AAC
Cuts at: 7221 7221
Size: 9631
KasI G'GCGC_C
Cuts at: 2682 2682
Size: 9631
KpnI G_GTAC'C
Cuts at: 6956 6956
Size: 9631
MfeI C'AATT_G
Cuts at: 2612 7230 2612
Size: 4618 5013
MluI A'CGCG_T
Cuts at: 9507 9507
Size: 9631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4304 6505 8745 8929 1305
Size: 2999 2201 2240 184 2007
Fragments arranged by size:
2999 2240 2201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2846 2988 4294 5024 5582
Size: 54 1425 343 1014 142 1306 730 558
Cuts at: 5582 10
Size: 4059
Fragments arranged by size:
4059 1425 1306 1014 730 558 343 142
54
MslI CAynn'nnrTG
Cuts at: 518 1166 4135 4674 5360 6587 6590 7589 7948
Size: 648 2969 539 686 1227 3 999 359
Cuts at: 7948 8107 518
Size: 159 2042
Fragments arranged by size:
2969 2042 1227 999 686 648 539 359
159 3
MspA1I CmG'CkG
Cuts at: 1606 2199 2809 3403 3694 3794 4201 4608 4786
Size: 593 610 594 291 100 407 407 178
Cuts at: 4786 5376 6232 6778 6790 7047 7724 8872 9117
Size: 590 856 546 12 257 677 1148 245
Cuts at: 9117 1606
Size: 2120
Fragments arranged by size:
2120 1148 856 677 610 594 593 590
546 407 407 291 257 245 178 100
12
NarI GG'CG_CC
Cuts at: 2683 2683
Size: 9631
NcoI C'CATG_G
Cuts at: 513 1065 4000 513
Size: 552 2935 6144
Fragments arranged by size:
6144 2935 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9631
NheI G'CTAG_C
Cuts at: 1052 2092 1052
Size: 1040 8591
NotI GC'GGCC_GC
Cuts at: 7078 7078
Size: 9631
NsiI A_TGCA'T
Cuts at: 6597 6597
Size: 9631
NspI r_CATG'y
Cuts at: 1132 1229 3049 3341 3718 4697 5299 5359 5650
Size: 97 1820 292 377 979 602 60 291
Cuts at: 5650 6989 8534 1132
Size: 1339 1545 2229
Fragments arranged by size:
2229 1820 1545 1339 979 602 377 292
291 97 60
PasI CC'CwG_GG
Cuts at: 1348 4219 4681 5175 5242 5516 6898 1348
Size: 2871 462 494 67 274 1382 4081
Fragments arranged by size:
4081 2871 1382 494 462 274 67
PciI A'CATG_T
Cuts at: 5646 8530 5646
Size: 2884 6747
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2360 2787 3049 3954 5233 6034 1108
Size: 1252 427 262 905 1279 801 4705
Fragments arranged by size:
4705 1279 1252 905 801 427 262
PfoI T'CCnGG_A
Cuts at: 3885 4455 4704 5043 5340 6249 6618 3885
Size: 570 249 339 297 909 369 6898
Fragments arranged by size:
6898 909 570 369 339 297 249
PmeI GTTT'AAAC
Cuts at: 6935 6935
Size: 9631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6085 6117 7700 7732 9213 9245 6085
Size: 32 1583 32 1481 32 6471
Fragments arranged by size:
6471 1583 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2550 3909 4258 5905 2550
Size: 1359 349 1647 6276
Fragments arranged by size:
6276 1647 1359 349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9631
PsiI TTA'TAA
Cuts at: 7201 7201
Size: 9631
PspOMI G'GGCC_C
Cuts at: 6513 6513
Size: 9631
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5540 5572 5540
Size: 32 9599
PstI C_TGCA'G
Cuts at: 830 2115 2950 3330 4032 4368 5310 5505 6774
Size: 1285 835 380 702 336 942 195 1269
Cuts at: 6774 6983 830
Size: 209 3478
Fragments arranged by size:
3478 1285 1269 942 835 702 380 336
209 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9631
PvuII CAG'CTG
Cuts at: 2199 3403 3694 4608 4786 6790 2199
Size: 1204 291 914 178 2004 5040
Fragments arranged by size:
5040 2004 1204 914 291 178
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 9631
SacI G_AGCT'C
Cuts at: 4045 4181 4577 6113 6786 4045
Size: 136 396 1536 673 6890
Fragments arranged by size:
6890 1536 673 396 136
SalI G'TCGA_C
Cuts at: 1936 6973 1936
Size: 5037 4594
SanDI GG'GwC_CC
Cuts at: 2550 2550
Size: 9631
SapI GCTCTTCn'nnn_
Cuts at: 4033 6284 4033
Size: 2251 7380
SbfI CC_TGCA'GG
Cuts at: 6983 6983
Size: 9631
ScaI AGT'ACT
Cuts at: 7879 9591 7879
Size: 1712 7919
SexAI A'CCwGG_T
Cuts at: 3807 3879 3807
Size: 72 9559
SfcI C'TryA_G
Cuts at: 826 1045 2111 2946 3222 3326 3398 4028 4364
Size: 219 1066 835 276 104 72 630 336
Cuts at: 4364 5306 5501 6770 6979 8114 8795 8986 826
Size: 942 195 1269 209 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 1066 942 835 681 630
336 276 219 209 195 191 104 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 5030 5030
Size: 9631
SfoI GGC'GCC
Cuts at: 2684 2684
Size: 9631
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9631
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9631
SmaI CCC'GGG
Cuts at: 1872 3862 6335 6458 6958 1872
Size: 1990 2473 123 500 4545
Fragments arranged by size:
4545 2473 1990 500 123
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 4864 4907 5909 6104 7735
Size: 197 926 312 2609 43 1002 195 1631
Cuts at: 7735 8636 8898 820
Size: 901 262 1553
Fragments arranged by size:
2609 1631 1553 1002 926 901 312 262
197 195 43
SnaBI TAC'GTA
Cuts at: 493 6928 7422 493
Size: 6435 494 2702
Fragments arranged by size:
6435 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9631
SphI G_CATG'C
Cuts at: 1132 3341 3718 4697 6989 1132
Size: 2209 377 979 2292 3774
Fragments arranged by size:
3774 2292 2209 979 377
SspI AAT'ATT
Cuts at: 5 52 7555 5
Size: 47 7503 2081
Fragments arranged by size:
7503 2081 47
StuI AGG'CCT
Cuts at: 1517 3502 3893 5935 1517
Size: 1985 391 2042 5213
Fragments arranged by size:
5213 2042 1985 391
StyI C'CwwG_G
Cuts at: 513 1065 2347 3342 4000 6759 7070 513
Size: 552 1282 995 658 2759 311 3074
Fragments arranged by size:
3074 2759 1282 995 658 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2264 3272 3275 3950 5234 5622 5852
Size: 1255 684 1008 3 675 1284 388 230
Cuts at: 5852 6462 7658 7675 7828 8013 8458 325
Size: 610 1196 17 153 185 445 1498
Fragments arranged by size:
1498 1284 1255 1196 1008 684 675 610
445 388 230 185 153 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3357 3819 4506
Size: 274 80 33 51 1443 1380 462 687
Cuts at: 4506 6432 7877 9589 96
Size: 1926 1445 1712 138
Fragments arranged by size:
1926 1712 1445 1443 1380 687 462 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2327 2359 2826 2858 4323 4355 6330
Size: 32 253 32 467 32 1465 32 1975
Cuts at: 6330 6362 6528 6560 9591 9623 2042
Size: 32 166 32 3031 32 2050
Fragments arranged by size:
3031 2050 1975 1465 467 253 166 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3881 4355 1316
Size: 2565 474 6592
Fragments arranged by size:
6592 2565 474
XbaI T'CTAG_A
Cuts at: 6967 6967
Size: 9631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3805 5453 5590 5660 3805
Size: 1648 137 70 7776
Fragments arranged by size:
7776 1648 137 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9631
XmaI C'CCGG_G
Cuts at: 1870 3860 6333 6456 6956 1870
Size: 1990 2473 123 500 4545
Fragments arranged by size:
4545 2473 1990 500 123
XmnI GAAnn'nnTTC
Cuts at: 5105 6155 7760 5105
Size: 1050 1605 6976
Fragments arranged by size:
6976 1605 1050
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7439 276
Size: 53 83 186 936 5905 2468
Fragments arranged by size:
5905 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsmBI BstZ17I DraIII FseI
NaeI NgoMIV NruI PacI PmlI PspXI SacII SrfI
SwaI