Restriction map for
pFN21ASDB1439
(Circular) (Six-base) MAPSORT of: pFN21ASDB1439.seq Check: 4867 from: 1 to: 11548
pFN21ASDB1439 11548 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3244 6477 3244
Size: 3233 8315
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6321 9358 278
Size: 53 83 186 936 4785 3037 2468
Fragments arranged by size:
4785 3037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 8208 8827 8891 1937
Size: 619 5652 619 64 4594
Fragments arranged by size:
5652 4594 619 619 64
Acc65I G'GTAC_C
Cuts at: 4587 8869 4587
Size: 4282 7266
AclI AA'CG_TT
Cuts at: 1563 9675 10048 1563
Size: 8112 373 3063
Fragments arranged by size:
8112 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 2653 3275 3468 4088 4412 5357 5483
Size: 1010 11 622 193 620 324 945 126
Cuts at: 5483 5864 6256 6377 6876 7035 7809 8067 9611
Size: 381 392 121 499 159 774 258 1544
Cuts at: 9611 10995 11263 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1544 1384 1010 945 774 622 620
499 392 381 324 268 258 193 159
126 121 11
AfeI AGC'GCT
Cuts at: 2657 2657
Size: 11548
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11351
AflIII A'CryG_T
Cuts at: 1203 3289 3657 3687 5582 10447 11424 1203
Size: 2086 368 30 1895 4865 977 1327
Fragments arranged by size:
4865 2086 1895 1327 977 368 30
AgeI A'CCGG_T
Cuts at: 10379 10379
Size: 11548
AhdI GACnn_n'nnGTC
Cuts at: 8784 10277 8784
Size: 1493 10055
AleI CACnn'nnGTG
Cuts at: 1166 7052 7991 1166
Size: 5886 939 4723
Fragments arranged by size:
5886 4723 939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11548
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 4612 4644 5697 5729 3644
Size: 32 936 32 1053 32 9463
Fragments arranged by size:
9463 1053 936 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3151 6094 9291 10863 3151
Size: 2943 3197 1572 3836
Fragments arranged by size:
3836 3197 2943 1572
ApaI G_GGCC'C
Cuts at: 3330 3479 6800 3330
Size: 149 3321 8078
Fragments arranged by size:
8078 3321 149
ApaLI G'TGCA_C
Cuts at: 9605 10761 9605
Size: 1156 10392
ApoI r'AATT_y
Cuts at: 1493 2871 4520 5811 6582 6668 6699 8501 8761
Size: 1378 1649 1291 771 86 31 1802 260
Cuts at: 8761 8857 9083 9235 11344 1493
Size: 96 226 152 2109 1697
Fragments arranged by size:
2109 1802 1697 1649 1378 1291 771 260
226 152 96 86 31
AseI AT'TA_AT
Cuts at: 160 1032 10102 160
Size: 872 9070 1606
Fragments arranged by size:
9070 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11548
AvaI C'yCGr_G
Cuts at: 1870 1943 3002 3018 3480 5308 6530 6968 8808
Size: 73 1059 16 462 1828 1222 438 1840
Cuts at: 8808 8873 1870
Size: 65 4545
Fragments arranged by size:
4545 1840 1828 1222 1059 462 438 73
65 16
BamHI G'GATC_C
Cuts at: 3091 4738 8878 9245 3091
Size: 1647 4140 367 5394
Fragments arranged by size:
5394 4140 1647 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2726 4587 5447 5992 6536
Size: 325 217 627 939 1861 860 545 544
Cuts at: 6536 7868 7946 8459 8477 8869 10324 618
Size: 1332 78 513 18 392 1455 1842
Fragments arranged by size:
1861 1842 1455 1332 939 860 627 545
544 513 392 325 217 78 18
BanII G_rGCy'C
Cuts at: 1391 3330 3479 4702 4719 4842 6800 6968 7181
Size: 1939 149 1223 17 123 1958 168 213
Cuts at: 7181 7749 7761 8313 8867 1391
Size: 568 12 552 554 4072
Fragments arranged by size:
4072 1958 1939 1223 568 554 552 213
168 149 123 17 12
BbeI G_GCGC'C
Cuts at: 5996 8463 5996
Size: 2467 9081
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 3051 6413 6864 7519 928
Size: 971 852 300 3362 451 655 4957
Fragments arranged by size:
4957 3362 971 852 655 451 300
BbvCI CC'TCA_GC
Cuts at: 2209 7508 2209
Size: 5299 6249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2072 2106 5910 5944 7243 7277 8153
Size: 34 135 34 3804 34 1299 34 876
Cuts at: 8153 8187 9739 9773 1903
Size: 34 1552 34 3678
Fragments arranged by size:
3804 3678 1552 1299 876 135 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3243 3702 6645 9440 10650 3243
Size: 459 2943 2795 1210 4141
Fragments arranged by size:
4141 2943 2795 1210 459
BclI T'GATC_A
Cuts at: 1548 2565 3372 3966 4594 1548
Size: 1017 807 594 628 8502
Fragments arranged by size:
8502 1017 807 628 594
BfrBI ATG'CAT
Cuts at: 3137 4183 3137
Size: 1046 10502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4301 7313 7400
Size: 107 122 71 631 605 2629 3012 87
Cuts at: 7400 8226 10159 136
Size: 826 1933 1525
Fragments arranged by size:
3012 2629 1933 1525 826 631 605 122
107 87 71
BglII A'GATC_T
Cuts at: 2834 3333 11543 2834
Size: 499 8210 2839
Fragments arranged by size:
8210 2839 499
BlpI GC'TnA_GC
Cuts at: 4274 4386 5085 5892 8965 4274
Size: 112 699 807 3073 6857
Fragments arranged by size:
6857 3073 807 699 112
Bme1580I G_kGCm'C
Cuts at: 1790 2729 3330 3479 3637 6041 6530 6800 7871
Size: 939 601 149 158 2404 489 270 1071
Cuts at: 7871 8480 9609 10765 1790
Size: 609 1129 1156 2573
Fragments arranged by size:
2573 2404 1156 1129 1071 939 609 601
489 270 158 149
BmgBI CAC'GTC
Cuts at: 1318 3751 5596 1318
Size: 2433 1845 7270
Fragments arranged by size:
7270 2433 1845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6716 7201 7645
Size: 416 53 83 399 24 5299 485 444
Cuts at: 7645 7989 9279 10237 442
Size: 344 1290 958 1753
Fragments arranged by size:
5299 1753 1290 958 485 444 416 399
344 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6734 7042 7633 8740 6734
Size: 308 591 1107 9542
Fragments arranged by size:
9542 1107 591 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 2672 3173 3538 3593 3668 3891 3908
Size: 977 456 501 365 55 75 223 17
Cuts at: 3908 5162 5595 5792 6062 7142 7275 7858 7935
Size: 1254 433 197 270 1080 133 583 77
Cuts at: 7935 8200 8624 8838 10208 1239
Size: 265 424 214 1370 2579
Fragments arranged by size:
2579 1370 1254 1080 977 583 501 456
433 424 365 270 265 223 214 197
133 77 75 55 17
Bpu10I CC'TnA_GC
Cuts at: 2209 5199 5347 5443 7490 7508 2209
Size: 2990 148 96 2047 18 6249
Fragments arranged by size:
6249 2990 2047 148 96 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2559 2802 7855 8477 9673 10538 10836 2559
Size: 243 5053 622 1196 865 298 3271
Fragments arranged by size:
5053 3271 1196 865 622 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2945 4067 5801 8656 10211 882
Size: 617 1446 1122 1734 2855 1555 2219
Fragments arranged by size:
2855 2219 1734 1555 1446 1122 617
BsaAI yAC'GTr
Cuts at: 493 1204 3721 5583 8232 8845 9339 493
Size: 711 2517 1862 2649 613 494 2702
Fragments arranged by size:
2702 2649 2517 1862 711 613 494
BsaBI GATnn'nnATC
Cuts at: 2972 4154 4175 11542 2972
Size: 1182 21 7367 2978
Fragments arranged by size:
7367 2978 1182 21
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5993 6318 8460 9355
Size: 53 83 186 936 4460 325 2142 895
Cuts at: 9355 9737 10372 275
Size: 382 635 1451
Fragments arranged by size:
4460 2142 1451 936 895 635 382 325
186 83 53
BsaWI w'CCGG_w
Cuts at: 2439 3825 4417 6373 7479 9981 10379 10653 10800
Size: 1386 592 1956 1106 2502 398 274 147
Cuts at: 10800 11211 2439
Size: 411 2776
Fragments arranged by size:
2776 2502 1956 1386 1106 592 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4007 4037 4043 4073 4121
Size: 30 165 30 2604 30 6 30 48
Cuts at: 4121 4151 1178
Size: 30 8575
Fragments arranged by size:
8575 2604 165 48 30 30 30 30
30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 5144 5789 6986 7625 8644 8647 8650
Size: 1162 2796 645 1197 639 1019 3 3
Cuts at: 8650 8675 8678 8732 1186
Size: 25 3 54 4002
Fragments arranged by size:
4002 2796 1197 1162 1019 645 639 54
25 3 3 3
BseYI C'CCAG_C
Cuts at: 4922 5106 5136 6435 7102 7238 7360 7385 7879
Size: 184 30 1299 667 136 122 25 494
Cuts at: 7879 10751 11247 4922
Size: 2872 496 5223
Fragments arranged by size:
5223 2872 1299 667 496 494 184 136
122 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3914 6107 6511 8633 8786 1163
Size: 573 2178 2193 404 2122 153 3925
Fragments arranged by size:
3925 2193 2178 2122 573 404 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2894 8998 9759 10787 1540
Size: 275 186 893 6104 761 1028 2301
Fragments arranged by size:
6104 2301 1028 893 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2714 3027 4749 4986 5091 6159 7617 9609 9694
Size: 313 1722 237 105 1068 1458 1992 85
Cuts at: 9694 10765 2714
Size: 1071 3497
Fragments arranged by size:
3497 1992 1722 1458 1071 1068 313 237
105 85
BsmI GAATG_Cn'
Cuts at: 2158 2186 2223 2371 3265 4901 9059 9152 2158
Size: 28 37 148 894 1636 4158 93 4554
Fragments arranged by size:
4554 4158 1636 894 148 93 37 28
BsmBI CGTCTCn'nnnn_
Cuts at: 2671 5305 5771 2671
Size: 2634 466 8448
Fragments arranged by size:
8448 2634 466
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2714 2729 3027 3330 3479 3637 4702
Size: 399 924 15 298 303 149 158 1065
Cuts at: 4702 4719 4749 4842 4986 5091 6041 6159 6530
Size: 17 30 93 144 105 950 118 371
Cuts at: 6530 6800 6968 7181 7617 7749 7761 7871 8313
Size: 270 168 213 436 132 12 110 442
Cuts at: 8313 8480 8867 9609 9694 10765 1391
Size: 167 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 1065 950 924 742 442 436
399 387 371 303 298 270 213 168
167 158 149 144 132 118 110 105
93 85 30 17 15 12
BspEI T'CCGG_A
Cuts at: 2439 6373 11211 2439
Size: 3934 4838 2776
Fragments arranged by size:
4838 3934 2776
BspHI T'CATG_A
Cuts at: 2176 2361 3963 6780 7218 8788 9437 11167 2176
Size: 185 1602 2817 438 1570 649 1730 2557
Fragments arranged by size:
2817 2557 1730 1602 1570 649 438 185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3244 4350 5558 6477 8562 8903 844
Size: 2400 1106 1208 919 2085 341 3489
Fragments arranged by size:
3489 2400 2085 1208 1106 919 341
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 6792 7135 9435 1394
Size: 429 341 4628 343 2300 3507
Fragments arranged by size:
4628 3507 2300 429 343 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 2254 5241 5409 5418 10043 10217 65
Size: 1183 1006 2987 168 9 4625 174 1396
Fragments arranged by size:
4625 2987 1396 1183 1006 174 168 9
BsrFI r'CCGG_y
Cuts at: 1700 1810 5995 7949 10192 10379 1700
Size: 110 4185 1954 2243 187 2869
Fragments arranged by size:
4185 2869 2243 1954 187 110
BsrGI T'GTAC_A
Cuts at: 96 3219 96
Size: 3123 8425
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11548
BssSI C'ACGA_G
Cuts at: 2108 9608 10620 2108
Size: 7500 1012 3036
Fragments arranged by size:
7500 3036 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2908 4549 6094 6154 6351 794
Size: 2114 1641 1545 60 197 5991
Fragments arranged by size:
5991 2114 1641 1545 197 60
BstBI TT'CG_AA
Cuts at: 2007 10352 2007
Size: 8345 3203
BstEII G'GTnAC_C
Cuts at: 1184 2917 7195 1184
Size: 1733 4278 5537
Fragments arranged by size:
5537 4278 1733
BstXI CCAn_nnnn'nTGG
Cuts at: 2387 4632 7054 7672 8323 2387
Size: 2245 2422 618 651 5612
Fragments arranged by size:
5612 2422 2245 651 618
BstYI r'GATC_y
Cuts at: 1972 2238 2834 3091 3333 3732 4011 4738 5326
Size: 266 596 257 242 399 279 727 588
Cuts at: 5326 8673 8878 9245 9630 9647 11099 11185 11197
Size: 3347 205 367 385 17 1452 86 12
Cuts at: 11197 11543 1972
Size: 346 1977
Fragments arranged by size:
3347 1977 1452 727 596 588 399 385
367 346 279 266 257 242 205 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 6954 7999 6954
Size: 1045 10503
BtgI C'CryG_G
Cuts at: 513 1065 2022 2225 3813 4506 7665 513
Size: 552 957 203 1588 693 3159 4396
Fragments arranged by size:
4396 3159 1588 957 693 552 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2216 2614 8254 487
Size: 683 56 990 398 5640 3781
Fragments arranged by size:
5640 3781 990 683 398 56
BtsI GCAGTG_nn'
Cuts at: 4110 4245 4487 5545 6298 7316 7447 7890 8614
Size: 135 242 1058 753 1018 131 443 724
Cuts at: 8614 9064 9858 9878 4110
Size: 450 794 20 5780
Fragments arranged by size:
5780 1058 1018 794 753 724 450 443
242 135 131 20
ClaI AT'CG_AT
Cuts at: 1554 8091 1554
Size: 6537 5011
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5667 5702 529
Size: 35 5103 35 6375
Fragments arranged by size:
6375 5103 35 35
DraI TTT'AAA
Cuts at: 8852 8852
Size: 11548
DraIII CAC_nnn'GTG
Cuts at: 5583 5583
Size: 11548
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2552 5975 10555 809
Size: 507 1236 3423 4580 1802
Fragments arranged by size:
4580 3423 1802 1236 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2389 2891 3504
Size: 54 1425 50 275 18 559 502 613
Cuts at: 3504 4846 6772 8442 8995 9884 11513 8
Size: 1342 1926 1670 553 889 1629 43
Fragments arranged by size:
1926 1670 1629 1425 1342 889 613 559
553 502 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2891 8995 1537
Size: 275 1079 6104 4090
Fragments arranged by size:
6104 4090 1079 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2461 2786 4031 4226 4455 5187 5462 5826
Size: 1108 325 1245 195 229 732 275 364
Cuts at: 5826 9478 1353
Size: 3652 3423
Fragments arranged by size:
3652 3423 1245 1108 732 364 325 275
229 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3542 3914 10135 10509 10655 387
Size: 1064 138 1953 372 6221 374 146 1280
Fragments arranged by size:
6221 1953 1280 1064 374 372 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2216 2642 2653 2672 3173 3275 3468
Size: 393 584 426 11 19 501 102 193
Cuts at: 3468 3538 3593 3668 3891 3908 4088 4412 5162
Size: 70 55 75 223 17 180 324 750
Cuts at: 5162 5357 5483 5595 5792 5864 6062 6256 6377
Size: 195 126 112 197 72 198 194 121
Cuts at: 6377 6876 7035 7142 7275 7809 7858 7935 8067
Size: 499 159 107 133 534 49 77 132
Cuts at: 8067 8200 8624 8838 9611 10208 10995 11263 1239
Size: 133 424 214 773 597 787 268 1524
Fragments arranged by size:
1524 787 773 750 597 584 534 501
499 426 424 393 324 268 223 214
198 197 195 194 193 180 159 133
133 132 126 121 112 107 102 77
75 72 70 55 49 19 17 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 3470 4595 4724 6953 7013 2720
Size: 750 1125 129 2229 60 7255
Fragments arranged by size:
7255 2229 1125 750 129 60
EcoO109I rG'GnC_Cy
Cuts at: 2644 2925 3326 3327 3475 3476 5203 5547 5870
Size: 281 401 1 148 1 1727 344 323
Cuts at: 5870 5936 6544 6796 6797 7552 7639 2644
Size: 66 608 252 1 755 87 6553
Fragments arranged by size:
6553 1727 755 608 401 344 323 281
252 148 87 66 1 1 1
EcoRI G'AATT_C
Cuts at: 2871 4520 8857 9235 2871
Size: 1649 4337 378 5184
Fragments arranged by size:
5184 4337 1649 378
EcoRV GAT'ATC
Cuts at: 2488 2488
Size: 11548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 4093 5488 6355 6386 8479 2110
Size: 1983 1395 867 31 2093 5179
Fragments arranged by size:
5179 2093 1983 1395 867 31
FspI TGC'GCA
Cuts at: 5504 10054 5504
Size: 4550 6998
HaeII r_GCGC'y
Cuts at: 1678 2621 2659 5892 5996 6095 7717 8463 10695
Size: 943 38 3233 104 99 1622 746 2232
Cuts at: 10695 1678
Size: 2531
Fragments arranged by size:
3233 2531 2232 1622 943 746 104 99
38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3691 3723 4121
Size: 32 135 32 985 32 1102 32 398
Cuts at: 4121 4153 5291 5304 5323 5336 5895 5927 7326
Size: 32 1138 13 19 13 559 32 1399
Cuts at: 7326 7358 8770 8802 10189 10221 10263 10295 1373
Size: 32 1412 32 1387 32 42 32 2626
Fragments arranged by size:
2626 1412 1399 1387 1138 1102 985 559
398 135 42 32 32 32 32 32
32 32 32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5914 6469 8828 8892 9138 669
Size: 988 281 3976 555 2359 64 246 3079
Fragments arranged by size:
3976 3079 2359 988 555 281 246 64
HindIII A'AGCT_T
Cuts at: 748 748
Size: 11548
HpaI GTT'AAC
Cuts at: 9138 9138
Size: 11548
KasI G'GCGC_C
Cuts at: 5992 8459 5992
Size: 2467 9081
KpnI G_GTAC'C
Cuts at: 4591 8873 4591
Size: 4282 7266
MfeI C'AATT_G
Cuts at: 9147 9147
Size: 11548
MluI A'CGCG_T
Cuts at: 11424 11424
Size: 11548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3892 5139 6136 6639 7882 10662 10846 1305
Size: 2587 1247 997 503 1243 2780 184 2007
Fragments arranged by size:
2780 2587 2007 1247 1243 997 503 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3506 4848 8444 10
Size: 54 1425 343 1674 1342 3596 3114
Fragments arranged by size:
3596 3114 1674 1425 1342 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3749 4031 4148 4256 4544 5363
Size: 648 2196 387 282 117 108 288 819
Cuts at: 5363 5598 6785 6869 7052 7076 7349 7670 7991
Size: 235 1187 84 183 24 273 321 321
Cuts at: 7991 8321 9506 9865 10024 518
Size: 330 1185 359 159 2042
Fragments arranged by size:
2196 2042 1187 1185 819 648 387 359
330 321 321 288 282 273 235 183
159 117 108 84 24
MspA1I CmG'CkG
Cuts at: 1606 3981 4385 4856 5110 5140 5556 6070 6351
Size: 2375 404 471 254 30 416 514 281
Cuts at: 6351 6439 8293 8964 9641 10789 11034 1606
Size: 88 1854 671 677 1148 245 2120
Fragments arranged by size:
2375 2120 1854 1148 677 671 514 471
416 404 281 254 245 88 30
NaeI GCC'GGC
Cuts at: 5997 7951 5997
Size: 1954 9594
NarI GG'CG_CC
Cuts at: 5993 8460 5993
Size: 2467 9081
NcoI C'CATG_G
Cuts at: 513 1065 2022 7665 513
Size: 552 957 5643 4396
Fragments arranged by size:
5643 4396 957 552
NdeI CA'TA_TG
Cuts at: 387 5749 387
Size: 5362 6186
NgoMIV G'CCGG_C
Cuts at: 5995 7949 5995
Size: 1954 9594
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11548
NotI GC'GGCC_GC
Cuts at: 8995 8995
Size: 11548
NsiI A_TGCA'T
Cuts at: 3139 4185 3139
Size: 1046 10502
NspI r_CATG'y
Cuts at: 1132 1229 3661 3691 5044 5095 5221 6086 6899
Size: 97 2432 30 1353 51 126 865 813
Cuts at: 6899 8347 8638 8906 10451 1132
Size: 1448 291 268 1545 2229
Fragments arranged by size:
2432 2229 1545 1448 1353 865 813 291
268 126 97 51 30
PasI CC'CwG_GG
Cuts at: 1348 2722 3385 5425 5987 1348
Size: 1374 663 2040 562 6909
Fragments arranged by size:
6909 2040 1374 663 562
PciI A'CATG_T
Cuts at: 3657 3687 10447 3657
Size: 30 6760 4758
Fragments arranged by size:
6760 4758 30
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 3151 4973 6341 7191 8410 1108
Size: 1320 723 1822 1368 850 1219 4246
Fragments arranged by size:
4246 1822 1368 1320 1219 850 723
PfoI T'CCnGG_A
Cuts at: 2293 2607 2649 3585 4125 4606 5622 7128 2293
Size: 314 42 936 540 481 1016 1506 6713
Fragments arranged by size:
6713 1506 1016 936 540 481 314 42
PmeI GTTT'AAAC
Cuts at: 8852 8852
Size: 11548
PmlI CAC'GTG
Cuts at: 3721 5583 3721
Size: 1862 9686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9617 9649 11130 11162 9617
Size: 32 1481 32 10003
Fragments arranged by size:
10003 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5203 5547 5870 5936 7639 5203
Size: 344 323 66 1703 9112
Fragments arranged by size:
9112 1703 344 323 66
PshAI GACnn'nnGTC
Cuts at: 1613 5793 6464 7589 1613
Size: 4180 671 1125 5572
Fragments arranged by size:
5572 4180 1125 671
PsiI TTA'TAA
Cuts at: 6647 9118 6647
Size: 2471 9077
PspOMI G'GGCC_C
Cuts at: 3326 3475 6796 3326
Size: 149 3321 8078
Fragments arranged by size:
8078 3321 149
PspXI vC'TCGA_Gb
Cuts at: 8808 8808
Size: 11548
PstI C_TGCA'G
Cuts at: 830 2639 3241 3858 4482 5555 6488 8900 830
Size: 1809 602 617 624 1073 933 2412 3478
Fragments arranged by size:
3478 2412 1809 1073 933 624 617 602
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11548
PvuII CAG'CTG
Cuts at: 4856 5110 5140 5556 6070 6439 4856
Size: 254 30 416 514 369 9965
Fragments arranged by size:
9965 514 416 369 254 30
SalI G'TCGA_C
Cuts at: 1936 8826 8890 1936
Size: 6890 64 4594
Fragments arranged by size:
6890 4594 64
SanDI GG'GwC_CC
Cuts at: 5936 5936
Size: 11548
SapI GCTCTTCn'nnn_
Cuts at: 2786 4031 4455 2786
Size: 1245 424 9879
Fragments arranged by size:
9879 1245 424
SbfI CC_TGCA'GG
Cuts at: 6488 8900 6488
Size: 2412 9136
ScaI AGT'ACT
Cuts at: 8723 9796 11508 8723
Size: 1073 1712 8763
Fragments arranged by size:
8763 1712 1073
SexAI A'CCwGG_T
Cuts at: 4118 4446 8748 4118
Size: 328 4302 6918
Fragments arranged by size:
6918 4302 328
SfcI C'TryA_G
Cuts at: 826 1045 2167 2635 2732 3237 3854 4312 4478
Size: 219 1122 468 97 505 617 458 166
Cuts at: 4478 5551 6484 8896 10031 10712 10903 826
Size: 1073 933 2412 1135 681 191 1471
Fragments arranged by size:
2412 1471 1135 1122 1073 933 681 617
505 468 458 219 191 166 97
SfoI GGC'GCC
Cuts at: 5994 8461 5994
Size: 2467 9081
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11548
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11548
SmaI CCC'GGG
Cuts at: 1872 8875 1872
Size: 7003 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2574 2817 5308 7834 8492 8808
Size: 197 926 631 243 2491 2526 658 316
Cuts at: 8808 9652 10553 10815 820
Size: 844 901 262 1553
Fragments arranged by size:
2526 2491 1553 926 901 844 658 631
316 262 243 197
SnaBI TAC'GTA
Cuts at: 493 8845 9339 493
Size: 8352 494 2702
Fragments arranged by size:
8352 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11548
SphI G_CATG'C
Cuts at: 1132 5221 6899 8347 8906 1132
Size: 4089 1678 1448 559 3774
Fragments arranged by size:
4089 3774 1678 1448 559
SspI AAT'ATT
Cuts at: 5 52 2850 8041 9472 5
Size: 47 2798 5191 1431 2081
Fragments arranged by size:
5191 2798 2081 1431 47
StuI AGG'CCT
Cuts at: 1517 3062 4470 5172 6815 1517
Size: 1545 1408 702 1643 6250
Fragments arranged by size:
6250 1643 1545 1408 702
StyI C'CwwG_G
Cuts at: 513 1065 2022 2386 4290 4631 5035 5262 5398
Size: 552 957 364 1904 341 404 227 136
Cuts at: 5398 5496 5791 6769 7014 7665 8987 513
Size: 98 295 978 245 651 1322 3074
Fragments arranged by size:
3074 1904 1322 978 957 651 552 404
364 341 295 245 227 136 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2905 3641 4727 4764 6021 6449 7183
Size: 1255 1325 736 1086 37 1257 428 734
Cuts at: 7183 9575 9592 9745 9930 10375 325
Size: 2392 17 153 185 445 1498
Fragments arranged by size:
2392 1498 1325 1257 1255 1086 736 734
445 428 185 153 37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3219 5487 6261
Size: 274 80 33 51 1443 1242 2268 774
Cuts at: 6261 7686 8721 9794 11506 96
Size: 1425 1035 1073 1712 138
Fragments arranged by size:
2268 1712 1443 1425 1242 1073 1035 774
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2096 2128 2402 2434 2897 2929 5524 5556 5569
Size: 32 274 32 463 32 2595 32 13
Cuts at: 5569 5601 6057 6089 7880 7912 11508 11540 2096
Size: 32 456 32 1791 32 3596 32 2104
Fragments arranged by size:
3596 2595 2104 1791 463 456 274 32
32 32 32 32 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11548
XbaI T'CTAG_A
Cuts at: 6847 8884 6847
Size: 2037 9511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4297 4919 5248 5293 5377 7166 7882 8320 4297
Size: 622 329 45 84 1789 716 438 7525
Fragments arranged by size:
7525 1789 716 622 438 329 84 45
XhoI C'TCGA_G
Cuts at: 1943 5308 8808 1943
Size: 3365 3500 4683
Fragments arranged by size:
4683 3500 3365
XmaI C'CCGG_G
Cuts at: 1870 8873 1870
Size: 7003 4545
XmnI GAAnn'nnTTC
Cuts at: 7299 9677 7299
Size: 2378 9170
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6319 9356 276
Size: 53 83 186 936 4785 3037 2468
Fragments arranged by size:
4785 3037 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I EcoICRI FseI FspAI
NruI PacI PsrI RsrII SacI SacII SfiI SrfI
SwaI