Restriction map for
pFN21ASDB1426
(Circular) (Six-base) MAPSORT of: pFN21ASDB1426.seq Check: 1799 from: 1 to: 6772
pFN21ASDB1426 6772 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4070 4070
Size: 6772
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3687 4582 278
Size: 53 83 186 936 2151 895 2468
Fragments arranged by size:
2468 2151 936 895 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4115 1937
Size: 2178 4594
Acc65I G'GTAC_C
Cuts at: 4093 4093
Size: 6772
AclI AA'CG_TT
Cuts at: 1563 4899 5272 1563
Size: 3336 373 3063
Fragments arranged by size:
3336 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2810 2959 3753 3811 4835 6219 6487 1632
Size: 1178 149 794 58 1024 1384 268 1917
Fragments arranged by size:
1917 1384 1178 1024 794 268 149 58
AfeI AGC'GCT
Cuts at: 2594 2594
Size: 6772
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6575
AflIII A'CryG_T
Cuts at: 1203 2403 5671 6648 1203
Size: 1200 3268 977 1327
Fragments arranged by size:
3268 1327 1200 977
AgeI A'CCGG_T
Cuts at: 5603 5603
Size: 6772
AhdI GACnn_n'nnGTC
Cuts at: 5501 5501
Size: 6772
AleI CACnn'nnGTG
Cuts at: 1166 2204 3764 1166
Size: 1038 1560 4174
Fragments arranged by size:
4174 1560 1038
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2264 2625 3380 1184
Size: 1080 361 755 4576
Fragments arranged by size:
4576 1080 755 361
AlwNI CAG_nnn'CTG
Cuts at: 2362 2560 3562 4515 6087 2362
Size: 198 1002 953 1572 3047
Fragments arranged by size:
3047 1572 1002 953 198
ApaI G_GGCC'C
Cuts at: 2567 2567
Size: 6772
ApaLI G'TGCA_C
Cuts at: 4829 5985 4829
Size: 1156 5616
ApoI r'AATT_y
Cuts at: 1493 4081 4307 4459 6568 1493
Size: 2588 226 152 2109 1697
Fragments arranged by size:
2588 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5326 160
Size: 872 4294 1606
Fragments arranged by size:
4294 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
AvaI C'yCGr_G
Cuts at: 1870 1943 2269 2504 2775 3294 3307 4097 1870
Size: 73 326 235 271 519 13 790 4545
Fragments arranged by size:
4545 790 519 326 271 235 73 13
BamHI G'GATC_C
Cuts at: 3071 4102 4469 3071
Size: 1031 367 5374
Fragments arranged by size:
5374 1031 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2166 2582 2609 2654 3146
Size: 325 217 627 379 416 27 45 492
Cuts at: 3146 4024 4093 5548 618
Size: 878 69 1455 1842
Fragments arranged by size:
1842 1455 878 627 492 416 379 325
217 69 45 27
BanII G_rGCy'C
Cuts at: 1391 2420 2519 2567 2627 2729 3272 3487 3978
Size: 1029 99 48 60 102 543 215 491
Cuts at: 3978 4091 1391
Size: 113 4072
Fragments arranged by size:
4072 1029 543 491 215 113 102 99
60 48
BbeI G_GCGC'C
Cuts at: 2170 2170
Size: 6772
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2231 3051 928
Size: 971 332 820 4649
Fragments arranged by size:
4649 971 820 332
BbvCI CC'TCA_GC
Cuts at: 2456 2456
Size: 6772
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4963 4997 1903
Size: 34 3026 34 3678
Fragments arranged by size:
3678 3026 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2989 4664 5874 2989
Size: 1675 1210 3887
Fragments arranged by size:
3887 1675 1210
BclI T'GATC_A
Cuts at: 1548 3384 1548
Size: 1836 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2536 2724 5383
Size: 107 122 71 631 605 864 188 2659
Cuts at: 5383 136
Size: 1525
Fragments arranged by size:
2659 1525 864 631 605 188 122 107
71
BglII A'GATC_T
Cuts at: 2055 6767 2055
Size: 4712 2060
BlpI GC'TnA_GC
Cuts at: 2728 4189 2728
Size: 1461 5311
Bme1580I G_kGCm'C
Cuts at: 1790 2356 2474 2567 2657 3151 4029 4833 5989
Size: 566 118 93 90 494 878 804 1156
Cuts at: 5989 1790
Size: 2573
Fragments arranged by size:
2573 1156 878 804 566 494 118 93
90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6772
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2395 2416 2935
Size: 416 53 83 399 24 978 21 519
Cuts at: 2935 3833 3902 4503 5461 442
Size: 898 69 601 958 1753
Fragments arranged by size:
1753 978 958 898 601 519 416 399
83 69 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6772
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3322 3503 3322
Size: 181 6591
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2396 3727 3947 5432 1239
Size: 959 198 1331 220 1485 2579
Fragments arranged by size:
2579 1485 1331 959 220 198
Bpu10I CC'TnA_GC
Cuts at: 2456 3203 3579 2456
Size: 747 376 5649
Fragments arranged by size:
5649 747 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4897 5762 6060 4897
Size: 865 298 5609
Fragments arranged by size:
5609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3783 3907 5435 882
Size: 617 2284 124 1528 2219
Fragments arranged by size:
2284 2219 1528 617 124
BsaAI yAC'GTr
Cuts at: 493 1204 3253 4069 4563 493
Size: 711 2049 816 494 2702
Fragments arranged by size:
2702 2049 816 711 494
BsaBI GATnn'nnATC
Cuts at: 6766 6766
Size: 6772
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2167 3684 4579 4961
Size: 53 83 186 936 634 1517 895 382
Cuts at: 4961 5596 275
Size: 635 1451
Fragments arranged by size:
1517 1451 936 895 635 634 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5205 5603 5877 6024 6435 5205
Size: 398 274 147 411 5542
Fragments arranged by size:
5542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2824 2854 3485 3515 3806
Size: 30 165 30 1421 30 631 30 291
Cuts at: 3806 3836 3887 3917 1178
Size: 30 51 30 4033
Fragments arranged by size:
4033 1421 631 291 165 51 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2711 2878 3461 3495 3536 1186
Size: 1525 167 583 34 41 4422
Fragments arranged by size:
4422 1525 583 167 41 34
BseYI C'CCAG_C
Cuts at: 2371 2605 3064 5975 6471 2371
Size: 234 459 2911 496 2672
Fragments arranged by size:
2911 2672 496 459 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 1163
Size: 573 492 5707
Fragments arranged by size:
5707 573 492
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2900 4222 4983 6011 1540
Size: 275 186 899 1322 761 1028 2301
Fragments arranged by size:
2301 1322 1028 899 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2385 2627 3462 3670 3978 4833 4918 5989 2385
Size: 242 835 208 308 855 85 1071 3168
Fragments arranged by size:
3168 1071 855 835 308 242 208 85
BsmI GAATG_Cn'
Cuts at: 4283 4376 4283
Size: 93 6679
BsmBI CGTCTCn'nnnn_
Cuts at: 3505 3505
Size: 6772
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2356 2385 2420 2474 2519 2567 2627
Size: 399 566 29 35 54 45 48 60
Cuts at: 2627 2657 2729 3151 3272 3462 3487 3670 3978
Size: 30 72 422 121 190 25 183 308
Cuts at: 3978 4029 4091 4833 4918 5989 1391
Size: 51 62 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 422 399 308 190
183 121 85 72 62 60 54 51
48 45 35 30 29 25
BspEI T'CCGG_A
Cuts at: 6435 6435
Size: 6772
BspHI T'CATG_A
Cuts at: 4661 6391 4661
Size: 1730 5042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3126 3579 4070 4127 844
Size: 2282 453 491 57 3489
Fragments arranged by size:
3489 2282 491 453 57
BsrBI CCG'CTC
Cuts at: 1394 1823 3931 4659 1394
Size: 429 2108 728 3507
Fragments arranged by size:
3507 2108 728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5267 5441 65
Size: 1183 4019 174 1396
Fragments arranged by size:
4019 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2343 2962 3054 3275 5416 5603 1700
Size: 110 533 619 92 221 2141 187 2869
Fragments arranged by size:
2869 2141 619 533 221 187 110 92
BsrGI T'GTAC_A
Cuts at: 96 3423 96
Size: 3327 3445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6772
BssSI C'ACGA_G
Cuts at: 4832 5844 4832
Size: 1012 5760
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3285 794
Size: 2491 4281
BstBI TT'CG_AA
Cuts at: 2007 5576 2007
Size: 3569 3203
BstEII G'GTnAC_C
Cuts at: 1184 3224 1184
Size: 2040 4732
BstXI CCAn_nnnn'nTGG
Cuts at: 2179 2539 3766 2179
Size: 360 1227 5185
Fragments arranged by size:
5185 1227 360
BstYI r'GATC_y
Cuts at: 1972 2055 3071 4102 4469 4854 4871 6323 6409
Size: 83 1016 1031 367 385 17 1452 86
Cuts at: 6409 6421 6767 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1031 1016 385 367 346 86
83 17 12
Bsu36I CC'TnA_GG
Cuts at: 3341 3341
Size: 6772
BtgI C'CryG_G
Cuts at: 513 1065 2178 2443 2498 2519 2649 3132 513
Size: 552 1113 265 55 21 130 483 4153
Fragments arranged by size:
4153 1113 552 483 265 130 55 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2261 487
Size: 683 56 1035 4998
Fragments arranged by size:
4998 1035 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2823 4288 5082 5102 2159
Size: 664 1465 794 20 3829
Fragments arranged by size:
3829 1465 794 664 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6772
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2910 2945 529
Size: 35 2346 35 4356
Fragments arranged by size:
4356 2346 35 35
DraI TTT'AAA
Cuts at: 4076 4076
Size: 6772
DraIII CAC_nnn'GTG
Cuts at: 3540 3540
Size: 6772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5779 809
Size: 507 4463 1802
Fragments arranged by size:
4463 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 2705 2931
Size: 54 1425 50 275 18 496 379 226
Cuts at: 2931 3033 3321 4219 5108 6737 8
Size: 102 288 898 889 1629 43
Fragments arranged by size:
1629 1425 898 889 496 379 288 275
226 102 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4219 1537
Size: 275 2407 4090
Fragments arranged by size:
4090 2407 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3374 3848 4702 1353
Size: 2021 474 854 3423
Fragments arranged by size:
3423 2021 854 474
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5359 5733 5879 387
Size: 1064 138 3770 374 146 1280
Fragments arranged by size:
3770 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2625 3976 2625
Size: 1351 5421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2396 2810 2959 3727 3753 3811
Size: 393 566 198 414 149 768 26 58
Cuts at: 3811 3947 4835 5432 6219 6487 1239
Size: 136 888 597 787 268 1524
Fragments arranged by size:
1524 888 787 768 597 566 414 393
268 198 149 136 58 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3122 3122
Size: 6772
EcoO109I rG'GnC_Cy
Cuts at: 2524 2564 2861 2970 3127 3559 3946 2524
Size: 40 297 109 157 432 387 5350
Fragments arranged by size:
5350 432 387 297 157 109 40
EcoRI G'AATT_C
Cuts at: 4081 4459 4081
Size: 378 6394
EcoRV GAT'ATC
Cuts at: 3772 3772
Size: 6772
FspI TGC'GCA
Cuts at: 5278 5278
Size: 6772
HaeII r_GCGC'y
Cuts at: 1678 2066 2170 2596 5919 1678
Size: 388 104 426 3323 2531
Fragments arranged by size:
3323 2531 426 388 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3485 3517 3761 3793 5413
Size: 32 135 32 1913 32 244 32 1620
Cuts at: 5413 5445 5487 5519 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1913 1620 244 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4116 4362 669
Size: 988 281 2178 246 3079
Fragments arranged by size:
3079 2178 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6772
HpaI GTT'AAC
Cuts at: 4362 4362
Size: 6772
KasI G'GCGC_C
Cuts at: 2166 2166
Size: 6772
KpnI G_GTAC'C
Cuts at: 4097 4097
Size: 6772
MfeI C'AATT_G
Cuts at: 4371 4371
Size: 6772
MluI A'CGCG_T
Cuts at: 6648 6648
Size: 6772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2337 2920 5886 6070 1305
Size: 1032 583 2966 184 2007
Fragments arranged by size:
2966 2007 1032 583 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2933 10
Size: 54 1425 343 1101 3849
Fragments arranged by size:
3849 1425 1101 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 3764 3791 4730 5089 5248 518
Size: 648 1038 1560 27 939 359 159 2042
Fragments arranged by size:
2042 1560 1038 939 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2460 2605 3068 4188 4865 6013 6258 1606
Size: 854 145 463 1120 677 1148 245 2120
Fragments arranged by size:
2120 1148 1120 854 677 463 245 145
NaeI GCC'GGC
Cuts at: 2345 2345
Size: 6772
NarI GG'CG_CC
Cuts at: 2167 2167
Size: 6772
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6220
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6772
NgoMIV G'CCGG_C
Cuts at: 2343 2343
Size: 6772
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6772
NotI GC'GGCC_GC
Cuts at: 4219 4219
Size: 6772
NruI TCG'CGA
Cuts at: 2194 2194
Size: 6772
NspI r_CATG'y
Cuts at: 1132 1229 2407 2815 3578 4130 5675 1132
Size: 97 1178 408 763 552 1545 2229
Fragments arranged by size:
2229 1545 1178 763 552 408 97
PasI CC'CwG_GG
Cuts at: 1348 2362 2560 1348
Size: 1014 198 5560
Fragments arranged by size:
5560 1014 198
PciI A'CATG_T
Cuts at: 2403 5671 2403
Size: 3268 3504
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2965 1108
Size: 1857 4915
PfoI T'CCnGG_A
Cuts at: 2151 3861 3924 2151
Size: 1710 63 4999
Fragments arranged by size:
4999 1710 63
PmeI GTTT'AAAC
Cuts at: 4076 4076
Size: 6772
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4841 4873 6354 6386 4841
Size: 32 1481 32 5227
Fragments arranged by size:
5227 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2524 2970 3127 3946 2524
Size: 446 157 819 5350
Fragments arranged by size:
5350 819 446 157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6772
PsiI TTA'TAA
Cuts at: 4342 4342
Size: 6772
PspOMI G'GGCC_C
Cuts at: 2563 2563
Size: 6772
PstI C_TGCA'G
Cuts at: 830 2604 2622 3477 3558 4002 4124 830
Size: 1774 18 855 81 444 122 3478
Fragments arranged by size:
3478 1774 855 444 122 81 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6772
PvuII CAG'CTG
Cuts at: 2460 2605 2460
Size: 145 6627
SacI G_AGCT'C
Cuts at: 2627 3978 2627
Size: 1351 5421
SalI G'TCGA_C
Cuts at: 1936 4114 1936
Size: 2178 4594
SanDI GG'GwC_CC
Cuts at: 3946 3946
Size: 6772
SapI GCTCTTCn'nnn_
Cuts at: 3374 3374
Size: 6772
SbfI CC_TGCA'GG
Cuts at: 3477 4124 3477
Size: 647 6125
ScaI AGT'ACT
Cuts at: 5020 6732 5020
Size: 1712 5060
SexAI A'CCwGG_T
Cuts at: 3435 3435
Size: 6772
SfcI C'TryA_G
Cuts at: 826 1045 2600 2618 3473 3554 3998 4120 5255
Size: 219 1555 18 855 81 444 122 1135
Cuts at: 5255 5936 6127 826
Size: 681 191 1471
Fragments arranged by size:
1555 1471 1135 855 681 444 219 191
122 81 18
SfoI GGC'GCC
Cuts at: 2168 2168
Size: 6772
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6772
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6772
SmaI CCC'GGG
Cuts at: 1872 2777 3296 4099 1872
Size: 905 519 803 4545
Fragments arranged by size:
4545 905 803 519
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4876 5777 6039 820
Size: 197 926 2933 901 262 1553
Fragments arranged by size:
2933 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4069 4563 493
Size: 3576 494 2702
Fragments arranged by size:
3576 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6772
SphI G_CATG'C
Cuts at: 1132 3578 4130 1132
Size: 2446 552 3774
Fragments arranged by size:
3774 2446 552
SspI AAT'ATT
Cuts at: 5 52 4696 5
Size: 47 4644 2081
Fragments arranged by size:
4644 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6772
StyI C'CwwG_G
Cuts at: 513 1065 2934 3141 4211 513
Size: 552 1869 207 1070 3074
Fragments arranged by size:
3074 1869 1070 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2597 3445 3892 4799 4816 4969 5154
Size: 1255 1017 848 447 907 17 153 185
Cuts at: 5154 5599 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1017 907 848 447 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3423 5018 6730
Size: 274 80 33 51 1443 1446 1595 1712
Cuts at: 6730 96
Size: 138
Fragments arranged by size:
1712 1595 1446 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2058 2090 2824 2856 3887 3919 6732 6764 2058
Size: 32 734 32 1031 32 2813 32 2066
Fragments arranged by size:
2813 2066 1031 734 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3497 1316
Size: 2181 4591
XbaI T'CTAG_A
Cuts at: 4108 4108
Size: 6772
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2227 3148 2179
Size: 48 921 5803
Fragments arranged by size:
5803 921 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6772
XmaI C'CCGG_G
Cuts at: 1870 2775 3294 4097 1870
Size: 905 519 803 4545
Fragments arranged by size:
4545 905 803 519
XmnI GAAnn'nnTTC
Cuts at: 4901 4901
Size: 6772
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3685 4580 276
Size: 53 83 186 936 2151 895 2468
Fragments arranged by size:
2468 2151 936 895 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I FalI
FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII
SacII SfiI SrfI SwaI