Restriction map for
pFN21ASDB1384
(Circular) (Six-base) MAPSORT of: pFN21ASDB1384.seq Check: 8454 from: 1 to: 6751
pFN21ASDB1384 6751 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3431 3431
Size: 6751
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4561 278
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3828 4094 1937
Size: 1891 266 4594
Fragments arranged by size:
4594 1891 266
Acc65I G'GTAC_C
Cuts at: 4072 4072
Size: 6751
AclI AA'CG_TT
Cuts at: 1563 4878 5251 1563
Size: 3315 373 3063
Fragments arranged by size:
3315 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3276 4814 6198 6466 1632
Size: 1644 1538 1384 268 1917
Fragments arranged by size:
1917 1644 1538 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6554
AflIII A'CryG_T
Cuts at: 1203 5650 6627 1203
Size: 4447 977 1327
Fragments arranged by size:
4447 1327 977
AgeI A'CCGG_T
Cuts at: 5582 5582
Size: 6751
AhdI GACnn_n'nnGTC
Cuts at: 5480 5480
Size: 6751
AleI CACnn'nnGTG
Cuts at: 1166 2033 2670 1166
Size: 867 637 5247
Fragments arranged by size:
5247 867 637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4014 1184
Size: 2830 3921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 6719
AlwNI CAG_nnn'CTG
Cuts at: 2194 3025 3160 3488 4494 6066 2194
Size: 831 135 328 1006 1572 2879
Fragments arranged by size:
2879 1572 1006 831 328 135
ApaLI G'TGCA_C
Cuts at: 4808 5964 4808
Size: 1156 5595
ApoI r'AATT_y
Cuts at: 1493 2698 3235 3405 3506 3613 4060 4286 4438
Size: 1205 537 170 101 107 447 226 152
Cuts at: 4438 6547 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1205 537 447 226 170 152
107 101
AseI AT'TA_AT
Cuts at: 160 1032 5305 160
Size: 872 4273 1606
Fragments arranged by size:
4273 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
AvaI C'yCGr_G
Cuts at: 1870 1943 4076 1870
Size: 73 2133 4545
Fragments arranged by size:
4545 2133 73
BamHI G'GATC_C
Cuts at: 4081 4448 4081
Size: 367 6384
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4072 5527 618
Size: 325 217 627 2285 1455 1842
Fragments arranged by size:
2285 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3359 4070 1391
Size: 1968 711 4072
Fragments arranged by size:
4072 1968 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3064 3195 928
Size: 971 1165 131 4484
Fragments arranged by size:
4484 1165 971 131
BbvCI CC'TCA_GC
Cuts at: 2358 3077 3447 3543 2358
Size: 719 370 96 5566
Fragments arranged by size:
5566 719 370 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4942 4976 1903
Size: 34 3005 34 3678
Fragments arranged by size:
3678 3005 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2880 4643 5853 2880
Size: 1763 1210 3778
Fragments arranged by size:
3778 1763 1210
BclI T'GATC_A
Cuts at: 1548 3490 1548
Size: 1942 4809
BfrBI ATG'CAT
Cuts at: 3703 3703
Size: 6751
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2912 5362 136
Size: 107 122 71 631 605 1240 2450 1525
Fragments arranged by size:
2450 1525 1240 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2642 6746 2642
Size: 4104 2647
BlpI GC'TnA_GC
Cuts at: 4168 4168
Size: 6751
Bme1580I G_kGCm'C
Cuts at: 1790 4812 5968 1790
Size: 3022 1156 2573
Fragments arranged by size:
3022 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6751
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3209 3561 4482
Size: 416 53 83 399 24 1792 352 921
Cuts at: 4482 5440 442
Size: 958 1753
Fragments arranged by size:
1792 1753 958 921 416 399 352 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 2308 2559 3225 5411 1239
Size: 871 198 251 666 2186 2579
Fragments arranged by size:
2579 2186 871 666 251 198
Bpu10I CC'TnA_GC
Cuts at: 2358 3077 3447 3543 2358
Size: 719 370 96 5566
Fragments arranged by size:
5566 719 370 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4876 5741 6039 4876
Size: 865 298 5588
Fragments arranged by size:
5588 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5414 882
Size: 617 3915 2219
Fragments arranged by size:
3915 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4048 4542 493
Size: 711 2844 494 2702
Fragments arranged by size:
2844 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 6745 6745
Size: 6751
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4558 4940 5575 275
Size: 53 83 186 936 3025 382 635 1451
Fragments arranged by size:
3025 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5184 5582 5856 6003 6414 5184
Size: 398 274 147 411 5521
Fragments arranged by size:
5521 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6526
Fragments arranged by size:
6526 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6751
BseYI C'CCAG_C
Cuts at: 5954 6450 5954
Size: 496 6255
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 2446 1163
Size: 573 386 324 5468
Fragments arranged by size:
5468 573 386 324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 4201 4962 5990 1540
Size: 275 186 33 2167 761 1028 2301
Fragments arranged by size:
2301 2167 1028 761 275 186 33
BsiHKAI G_wGCw'C
Cuts at: 4812 4897 5968 4812
Size: 85 1071 5595
Fragments arranged by size:
5595 1071 85
BsmI GAATG_Cn'
Cuts at: 3920 3988 4262 4355 3920
Size: 68 274 93 6316
Fragments arranged by size:
6316 274 93 68
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3359 4070 4812 4897 5968 1391
Size: 399 1569 711 742 85 1071 2174
Fragments arranged by size:
2174 1569 1071 742 711 399 85
BspEI T'CCGG_A
Cuts at: 6414 6414
Size: 6751
BspHI T'CATG_A
Cuts at: 4640 6370 4640
Size: 1730 5021
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2046 2510 2770 3431 4106 844
Size: 1202 464 260 661 675 3489
Fragments arranged by size:
3489 1202 675 661 464 260
BsrBI CCG'CTC
Cuts at: 1394 1823 4638 1394
Size: 429 2815 3507
Fragments arranged by size:
3507 2815 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2397 5246 5420 65
Size: 1183 1149 2849 174 1396
Fragments arranged by size:
2849 1396 1183 1149 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2040 2051 5395 5582 1700
Size: 110 230 11 3344 187 2869
Fragments arranged by size:
3344 2869 230 187 110 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6751
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6751
BssSI C'ACGA_G
Cuts at: 4811 5823 4811
Size: 1012 5739
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2368 794
Size: 1574 5177
BstBI TT'CG_AA
Cuts at: 2007 5555 2007
Size: 3548 3203
BstEII G'GTnAC_C
Cuts at: 1184 3432 1184
Size: 2248 4503
BstXI CCAn_nnnn'nTGG
Cuts at: 3089 3474 3089
Size: 385 6366
BstYI r'GATC_y
Cuts at: 1972 2642 4081 4448 4833 4850 6302 6388 6400
Size: 670 1439 367 385 17 1452 86 12
Cuts at: 6400 6746 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1439 670 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2022 2028 513
Size: 552 957 6 5236
Fragments arranged by size:
5236 957 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6012
Fragments arranged by size:
6012 683 56
BtsI GCAGTG_nn'
Cuts at: 2427 2751 3812 4267 5061 5081 2427
Size: 324 1061 455 794 20 4097
Fragments arranged by size:
4097 1061 794 455 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6716
DraI TTT'AAA
Cuts at: 3141 4055 3141
Size: 914 5837
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5758 809
Size: 507 4442 1802
Fragments arranged by size:
4442 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2031 4198
Size: 54 1425 50 275 18 195 6 2167
Cuts at: 4198 5087 6716 8
Size: 889 1629 43
Fragments arranged by size:
2167 1629 1425 889 275 195 54 50
43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2031 4198 1537
Size: 275 219 2167 4090
Fragments arranged by size:
4090 2167 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 2897 4681 1353
Size: 1544 1784 3423
Fragments arranged by size:
3423 1784 1544
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5338 5712 5858 387
Size: 1064 138 3749 374 146 1280
Fragments arranged by size:
3749 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 2308 2559 3225 3276 4814 5411
Size: 393 478 198 251 666 51 1538 597
Cuts at: 5411 6198 6466 1239
Size: 787 268 1524
Fragments arranged by size:
1538 1524 787 666 597 478 393 268
251 198 51
EcoO109I rG'GnC_Cy
Cuts at: 3022 3366 3022
Size: 344 6407
EcoRI G'AATT_C
Cuts at: 4060 4438 4060
Size: 378 6373
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3634 3634
Size: 6751
FspI TGC'GCA
Cuts at: 2496 5257 2496
Size: 2761 3990
HaeII r_GCGC'y
Cuts at: 1678 5898 1678
Size: 4220 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2370 2634 2666 3154
Size: 32 135 32 766 32 264 32 488
Cuts at: 3154 3186 5392 5424 5466 5498 1373
Size: 32 2206 32 42 32 2626
Fragments arranged by size:
2626 2206 766 488 264 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3810 3829 4095 4341 669
Size: 988 281 643 1229 19 266 246 3079
Fragments arranged by size:
3079 1229 988 643 281 266 246 19
HindIII A'AGCT_T
Cuts at: 748 2739 3842 748
Size: 1991 1103 3657
Fragments arranged by size:
3657 1991 1103
HpaI GTT'AAC
Cuts at: 2581 4341 2581
Size: 1760 4991
KpnI G_GTAC'C
Cuts at: 4076 4076
Size: 6751
MfeI C'AATT_G
Cuts at: 3103 4350 3103
Size: 1247 5504
MluI A'CGCG_T
Cuts at: 6627 6627
Size: 6751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5865 6049 1305
Size: 4560 184 2007
Fragments arranged by size:
4560 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 10
Size: 54 1425 343 195 4734
Fragments arranged by size:
4734 1425 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2033 2670 2756 2772 3981 4709 5068
Size: 648 867 637 86 16 1209 728 359
Cuts at: 5068 5227 518
Size: 159 2042
Fragments arranged by size:
2042 1209 867 728 648 637 359 159
86 16
MspA1I CmG'CkG
Cuts at: 1606 4167 4844 5992 6237 1606
Size: 2561 677 1148 245 2120
Fragments arranged by size:
2561 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2042 2053 2042
Size: 11 6740
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5242
Fragments arranged by size:
5242 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6751
NgoMIV G'CCGG_C
Cuts at: 2040 2051 2040
Size: 11 6740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6751
NotI GC'GGCC_GC
Cuts at: 4198 4198
Size: 6751
NsiI A_TGCA'T
Cuts at: 3705 3705
Size: 6751
NspI r_CATG'y
Cuts at: 1132 1229 4109 5654 1132
Size: 97 2880 1545 2229
Fragments arranged by size:
2880 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2431 1348
Size: 1083 5668
PciI A'CATG_T
Cuts at: 5650 5650
Size: 6751
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2742 1108
Size: 1634 5117
PmeI GTTT'AAAC
Cuts at: 4055 4055
Size: 6751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4820 4852 6333 6365 4820
Size: 32 1481 32 5206
Fragments arranged by size:
5206 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3022 3366 3022
Size: 344 6407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6751
PsiI TTA'TAA
Cuts at: 2153 4321 2153
Size: 2168 4583
PstI C_TGCA'G
Cuts at: 830 2422 4003 4103 830
Size: 1592 1581 100 3478
Fragments arranged by size:
3478 1592 1581 100
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6751
SalI G'TCGA_C
Cuts at: 1936 3827 4093 1936
Size: 1891 266 4594
Fragments arranged by size:
4594 1891 266
SanDI GG'GwC_CC
Cuts at: 3366 3366
Size: 6751
SbfI CC_TGCA'GG
Cuts at: 4103 4103
Size: 6751
ScaI AGT'ACT
Cuts at: 3836 4999 6711 3836
Size: 1163 1712 3876
Fragments arranged by size:
3876 1712 1163
SexAI A'CCwGG_T
Cuts at: 3085 3085
Size: 6751
SfcI C'TryA_G
Cuts at: 826 1045 2418 2554 2560 3999 4099 5234 5915
Size: 219 1373 136 6 1439 100 1135 681
Cuts at: 5915 6106 826
Size: 191 1471
Fragments arranged by size:
1471 1439 1373 1135 681 219 191 136
100 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6751
SmaI CCC'GGG
Cuts at: 1872 4078 1872
Size: 2206 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4855 5756 6018 820
Size: 197 926 2912 901 262 1553
Fragments arranged by size:
2912 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4048 4542 493
Size: 3555 494 2702
Fragments arranged by size:
3555 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6751
SphI G_CATG'C
Cuts at: 1132 4109 1132
Size: 2977 3774
SspI AAT'ATT
Cuts at: 5 52 3044 4675 5
Size: 47 2992 1631 2081
Fragments arranged by size:
2992 2081 1631 47
StuI AGG'CCT
Cuts at: 1517 3668 1517
Size: 2151 4600
StyI C'CwwG_G
Cuts at: 513 1065 2022 4190 513
Size: 552 957 2168 3074
Fragments arranged by size:
3074 2168 957 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3675 4778 4795 4948 5133 5578 325
Size: 1255 2095 1103 17 153 185 445 1498
Fragments arranged by size:
2095 1498 1255 1103 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 3834 4997
Size: 274 80 33 51 1443 462 1395 1163
Cuts at: 4997 6709 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1395 1163 462 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2057 2089 3873 3905 6711 6743 2057
Size: 32 1784 32 2806 32 2065
Fragments arranged by size:
2806 2065 1784 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6751
XbaI T'CTAG_A
Cuts at: 4087 4087
Size: 6751
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6751
XmaI C'CCGG_G
Cuts at: 1870 4076 1870
Size: 2206 4545
XmnI GAAnn'nnTTC
Cuts at: 4880 4880
Size: 6751
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4559 276
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SapI SfiI
SfoI SrfI SwaI XcmI