Restriction map for
pFN21ASDB0238
(Circular) (Six-base) MAPSORT of: pFN21ASDB0238.seq Check: 4951 from: 1 to: 8500
pFN21ASDB0238 8500 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3903 4989 3903
Size: 1086 7414
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6310 278
Size: 53 83 186 936 4774 2468
Fragments arranged by size:
4774 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5843 1937
Size: 3906 4594
Acc65I G'GTAC_C
Cuts at: 4986 5821 4986
Size: 835 7665
AclI AA'CG_TT
Cuts at: 1563 6627 7000 1563
Size: 5064 373 3063
Fragments arranged by size:
5064 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3413 3578 4738 4985 5140 6563 7947 8215
Size: 1781 165 1160 247 155 1423 1384 268
Cuts at: 8215 1632
Size: 1917
Fragments arranged by size:
1917 1781 1423 1384 1160 268 247 165
155
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8303
AflIII A'CryG_T
Cuts at: 1203 2043 4353 7399 8376 1203
Size: 840 2310 3046 977 1327
Fragments arranged by size:
3046 2310 1327 977 840
AgeI A'CCGG_T
Cuts at: 7331 7331
Size: 8500
AhdI GACnn_n'nnGTC
Cuts at: 7229 7229
Size: 8500
AleI CACnn'nnGTG
Cuts at: 1166 3221 4364 5501 1166
Size: 2055 1143 1137 4165
Fragments arranged by size:
4165 2055 1143 1137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 7117
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3429 3461 5291 5323 5660 5678 5692 5710 3429
Size: 32 1830 32 337 18 14 18 6219
Fragments arranged by size:
6219 1830 337 32 32 18 18 14
AlwNI CAG_nnn'CTG
Cuts at: 2070 2920 3403 6243 7815 2070
Size: 850 483 2840 1572 2755
Fragments arranged by size:
2840 2755 1572 850 483
ApaI G_GGCC'C
Cuts at: 3594 4308 4737 5024 3594
Size: 714 429 287 7070
Fragments arranged by size:
7070 714 429 287
ApaLI G'TGCA_C
Cuts at: 6557 7713 6557
Size: 1156 7344
ApoI r'AATT_y
Cuts at: 1493 3974 5809 6035 6187 8296 1493
Size: 2481 1835 226 152 2109 1697
Fragments arranged by size:
2481 2109 1835 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7054 160
Size: 872 6022 1606
Fragments arranged by size:
6022 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 4112 4784 5825 1870
Size: 73 147 2022 672 1041 4545
Fragments arranged by size:
4545 2022 1041 672 147 73
BamHI G'GATC_C
Cuts at: 3132 5830 6197 3132
Size: 2698 367 5435
Fragments arranged by size:
5435 2698 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2151 2384 2423 2666 3182
Size: 325 217 627 364 233 39 243 516
Cuts at: 3182 3744 3865 3994 4284 4639 4986 5313 5821
Size: 562 121 129 290 355 347 327 508
Cuts at: 5821 7276 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 562 516 508 364 355
347 327 325 290 243 233 217 129
121 39
BanII G_rGCy'C
Cuts at: 1391 3258 3594 3857 4122 4308 4422 4737 5024
Size: 1867 336 263 265 186 114 315 287
Cuts at: 5024 5243 5819 1391
Size: 219 576 4072
Fragments arranged by size:
4072 1867 576 336 315 287 265 263
219 186 114
BbeI G_GCGC'C
Cuts at: 2155 3186 3748 3998 4288 2155
Size: 1031 562 250 290 6367
Fragments arranged by size:
6367 1031 562 290 250
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2155 3945 5336 928
Size: 971 256 1790 1391 4092
Fragments arranged by size:
4092 1790 1391 971 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3355 3389 3586 3620 6691 6725 1903
Size: 34 1418 34 197 34 3071 34 3678
Fragments arranged by size:
3678 3071 1418 197 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 6392 7602 4556
Size: 1836 1210 5454
Fragments arranged by size:
5454 1836 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2592 2862 4857
Size: 107 122 71 631 605 920 270 1995
Cuts at: 4857 7111 136
Size: 2254 1525
Fragments arranged by size:
2254 1995 1525 920 631 605 270 122
107 71
BglII A'GATC_T
Cuts at: 2547 8495 2547
Size: 5948 2552
BlpI GC'TnA_GC
Cuts at: 5917 5917
Size: 8500
Bme1580I G_kGCm'C
Cuts at: 1790 2387 2428 2986 3390 3594 3835 4308 4371
Size: 597 41 558 404 204 241 473 63
Cuts at: 4371 4644 4737 5024 6561 7717 1790
Size: 273 93 287 1537 1156 2573
Fragments arranged by size:
2573 1537 1156 597 558 473 404 287
273 241 204 93 63 41
BmgBI CAC'GTC
Cuts at: 1318 4356 1318
Size: 3038 5462
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2853 2984 3204
Size: 416 53 83 399 24 1436 131 220
Cuts at: 3204 3384 6231 7189 442
Size: 180 2847 958 1753
Fragments arranged by size:
2847 1753 1436 958 416 399 220 180
131 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8500
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5376 5376
Size: 8500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2749 2828 3139 3467 3686 3946 4464 4600
Size: 1510 79 311 328 219 260 518 136
Cuts at: 4600 4721 4820 5579 7160 1239
Size: 121 99 759 1581 2579
Fragments arranged by size:
2579 1581 1510 759 518 328 311 260
219 136 121 99 79
Bpu10I CC'TnA_GC
Cuts at: 3194 4470 5752 3194
Size: 1276 1282 5942
Fragments arranged by size:
5942 1282 1276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2171 4037 4483 6625 7490 7788 2171
Size: 1866 446 2142 865 298 2883
Fragments arranged by size:
2883 2142 1866 865 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3471 3690 4135 4196 5528 7163 882
Size: 617 1972 219 445 61 1332 1635 2219
Fragments arranged by size:
2219 1972 1635 1332 617 445 219 61
BsaAI yAC'GTr
Cuts at: 493 1204 2044 3636 4224 4876 5797 6291 493
Size: 711 840 1592 588 652 921 494 2702
Fragments arranged by size:
2702 1592 921 840 711 652 588 494
BsaBI GATnn'nnATC
Cuts at: 8494 8494
Size: 8500
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2152 3183 3745 3995
Size: 53 83 186 936 619 1031 562 250
Cuts at: 3995 4285 6307 6689 7324 275
Size: 290 2022 382 635 1451
Fragments arranged by size:
2022 1451 1031 936 635 619 562 382
290 250 186 83 53
BsaWI w'CCGG_w
Cuts at: 2523 3168 3497 4236 6933 7331 7605 7752 8163
Size: 645 329 739 2697 398 274 147 411
Cuts at: 8163 2523
Size: 2860
Fragments arranged by size:
2860 2697 739 645 411 398 329 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2285 2315 3369 3399 4793
Size: 30 165 30 882 30 1054 30 1394
Cuts at: 4793 4823 4865 4895 5132 5162 5225 5255 5291
Size: 30 42 30 237 30 63 30 36
Cuts at: 5291 5321 1178
Size: 30 4357
Fragments arranged by size:
4357 1394 1054 882 237 165 63 42
36 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2048 2948 3158 5414 5588 1186
Size: 859 3 900 210 2256 174 4098
Fragments arranged by size:
4098 2256 900 859 210 174 3
BseYI C'CCAG_C
Cuts at: 2266 2380 3668 4121 7703 8199 2266
Size: 114 1288 453 3582 496 2567
Fragments arranged by size:
3582 2567 1288 496 453 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2532 3014 3224 3875 4298 4727
Size: 573 501 295 482 210 651 423 429
Cuts at: 4727 1163
Size: 4936
Fragments arranged by size:
4936 651 573 501 482 429 423 295
210
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3694 4526 5522 5950 6711
Size: 275 186 880 813 832 996 428 761
Cuts at: 6711 7739 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 996 880 832 813 761 428
275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 4866 4881 5243 6561 6646 7717 2619
Size: 2247 15 362 1318 85 1071 3402
Fragments arranged by size:
3402 2247 1318 1071 362 85 15
BsiWI C'GTAC_G
Cuts at: 5038 5038
Size: 8500
BsmI GAATG_Cn'
Cuts at: 3471 6011 6104 3471
Size: 2540 93 5867
Fragments arranged by size:
5867 2540 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5128 5128
Size: 8500
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2387 2428 2619 2986 3258 3390 3594
Size: 399 597 41 191 367 272 132 204
Cuts at: 3594 3835 3857 4122 4308 4371 4422 4644 4737
Size: 241 22 265 186 63 51 222 93
Cuts at: 4737 4866 4881 5024 5243 5819 6561 6646 7717
Size: 129 15 143 219 576 742 85 1071
Cuts at: 7717 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 597 576 399 367 272
265 241 222 219 204 191 186 143
132 129 93 85 63 51 41 22
15
BspEI T'CCGG_A
Cuts at: 2523 8163 2523
Size: 5640 2860
BspHI T'CATG_A
Cuts at: 6389 8119 6389
Size: 1730 6770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3897 3903 4989 5268 5855 844
Size: 3053 6 1086 279 587 3489
Fragments arranged by size:
3489 3053 1086 587 279 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 4234 6387 1394
Size: 429 749 1662 2153 3507
Fragments arranged by size:
3507 2153 1662 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5749 6995 7169 65
Size: 1183 4501 1246 174 1396
Fragments arranged by size:
4501 1396 1246 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3246 3862 4525 4868 7144 7331 1700
Size: 110 1436 616 663 343 2276 187 2869
Fragments arranged by size:
2869 2276 1436 663 616 343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8500
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8500
BssSI C'ACGA_G
Cuts at: 4299 6560 7572 4299
Size: 2261 1012 5227
Fragments arranged by size:
5227 2261 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2070 794
Size: 1276 7224
BstBI TT'CG_AA
Cuts at: 2007 7304 2007
Size: 5297 3203
BstEII G'GTnAC_C
Cuts at: 1184 3112 3223 3625 1184
Size: 1928 111 402 6059
Fragments arranged by size:
6059 1928 402 111
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 3053 3710 4213 5711 2029
Size: 1024 657 503 1498 4818
Fragments arranged by size:
4818 1498 1024 657 503
BstYI r'GATC_y
Cuts at: 1972 2085 2403 2526 2547 3132 3303 5070 5283
Size: 113 318 123 21 585 171 1767 213
Cuts at: 5283 5830 6197 6582 6599 8051 8137 8149 8495
Size: 547 367 385 17 1452 86 12 346
Cuts at: 8495 1972
Size: 1977
Fragments arranged by size:
1977 1767 1452 585 547 385 367 346
318 213 171 123 113 86 21 17
12
Bsu36I CC'TnA_GG
Cuts at: 4152 4290 4152
Size: 138 8362
BtgI C'CryG_G
Cuts at: 513 1065 2022 2147 2457 3006 3869 4558 5781
Size: 552 957 125 310 549 863 689 1223
Cuts at: 5781 513
Size: 3232
Fragments arranged by size:
3232 1223 957 863 689 552 549 310
125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2800 5586 487
Size: 683 56 1574 2786 3401
Fragments arranged by size:
3401 2786 1574 683 56
BtsI GCAGTG_nn'
Cuts at: 2986 3348 4286 5258 5638 6016 6810 6830 2986
Size: 362 938 972 380 378 794 20 4656
Fragments arranged by size:
4656 972 938 794 380 378 362 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8500
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8465
DraI TTT'AAA
Cuts at: 5804 5804
Size: 8500
DraIII CAC_nnn'GTG
Cuts at: 2113 2779 5253 2113
Size: 666 2474 5360
Fragments arranged by size:
5360 2474 666
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2705 3086 7507 809
Size: 507 1389 381 4421 1802
Fragments arranged by size:
4421 1802 1389 507 381
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2415 2435 2961
Size: 54 1425 50 275 18 585 20 526
Cuts at: 2961 3150 3264 3798 4523 4777 5646 5701 5947
Size: 189 114 534 725 254 869 55 246
Cuts at: 5947 6836 8465 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 869 725 585 534 526
275 254 246 189 114 55 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 4523 5947 1537
Size: 275 2711 1424 4090
Fragments arranged by size:
4090 2711 1424 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2256 2756 5184 6430 1353
Size: 903 500 2428 1246 3423
Fragments arranged by size:
3423 2428 1246 903 500
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7087 7461 7607 387
Size: 1064 138 5498 374 146 1280
Fragments arranged by size:
5498 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5241 5241
Size: 8500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2749 2828 3139 3413 3467 3578 3686
Size: 393 1117 79 311 274 54 111 108
Cuts at: 3686 3946 4464 4600 4721 4738 4820 4985 5140
Size: 260 518 136 121 17 82 165 155
Cuts at: 5140 5579 6563 7160 7947 8215 1239
Size: 439 984 597 787 268 1524
Fragments arranged by size:
1524 1117 984 787 597 518 439 393
311 274 268 260 165 155 136 121
111 108 82 79 54 17
EcoNI CCTnn'n_nnAGG
Cuts at: 4538 4898 4538
Size: 360 8140
EcoO109I rG'GnC_Cy
Cuts at: 3099 3462 3590 3591 4304 4305 4733 4936 5020
Size: 363 128 1 713 1 428 203 84
Cuts at: 5020 5021 5131 5257 5553 3099
Size: 1 110 126 296 6046
Fragments arranged by size:
6046 713 428 363 296 203 128 126
110 84 1 1 1
EcoRI G'AATT_C
Cuts at: 5809 6187 5809
Size: 378 8122
FspI TGC'GCA
Cuts at: 7006 7006
Size: 8500
HaeII r_GCGC'y
Cuts at: 1678 2036 2155 3186 3383 3748 3998 4288 7647
Size: 358 119 1031 197 365 250 290 3359
Cuts at: 7647 1678
Size: 2531
Fragments arranged by size:
3359 2531 1031 365 358 290 250 197
119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2618 2650 4793 4825 5537
Size: 32 135 32 1046 32 2143 32 712
Cuts at: 5537 5569 7141 7173 7215 7247 1373
Size: 32 1572 32 42 32 2626
Fragments arranged by size:
2626 2143 1572 1046 712 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4396 5844 6090 669
Size: 988 281 2458 1448 246 3079
Fragments arranged by size:
3079 2458 1448 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8500
HpaI GTT'AAC
Cuts at: 6090 6090
Size: 8500
KasI G'GCGC_C
Cuts at: 2151 3182 3744 3994 4284 2151
Size: 1031 562 250 290 6367
Fragments arranged by size:
6367 1031 562 290 250
KpnI G_GTAC'C
Cuts at: 4990 5825 4990
Size: 835 7665
MfeI C'AATT_G
Cuts at: 6099 6099
Size: 8500
MluI A'CGCG_T
Cuts at: 8376 8376
Size: 8500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 3909 4374 7614 7798 1305
Size: 1344 1260 465 3240 184 2007
Fragments arranged by size:
3240 2007 1344 1260 465 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2437 3152 3266 3800 4779
Size: 54 1425 343 605 715 114 534 979
Cuts at: 4779 5703 10
Size: 924 2807
Fragments arranged by size:
2807 1425 979 924 715 605 534 343
114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2390 3051 3221 4211 4364 5501
Size: 648 861 363 661 170 990 153 1137
Cuts at: 5501 6458 6817 6976 518
Size: 957 359 159 2042
Fragments arranged by size:
2042 1137 990 957 861 661 648 363
359 170 159 153
MspA1I CmG'CkG
Cuts at: 1606 2226 2361 2917 2955 3292 3792 4560 5102
Size: 620 135 556 38 337 500 768 542
Cuts at: 5102 5916 6593 7741 7986 1606
Size: 814 677 1148 245 2120
Fragments arranged by size:
2120 1148 814 768 677 620 556 542
500 337 245 135 38
NarI GG'CG_CC
Cuts at: 2152 3183 3745 3995 4285 2152
Size: 1031 562 250 290 6367
Fragments arranged by size:
6367 1031 562 290 250
NcoI C'CATG_G
Cuts at: 513 1065 2022 513
Size: 552 957 6991
Fragments arranged by size:
6991 957 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8500
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8500
NotI GC'GGCC_GC
Cuts at: 5947 5947
Size: 8500
NspI r_CATG'y
Cuts at: 1132 1229 2993 5858 7403 1132
Size: 97 1764 2865 1545 2229
Fragments arranged by size:
2865 2229 1764 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3466 3857 5626 1348
Size: 2118 391 1769 4222
Fragments arranged by size:
4222 2118 1769 391
PciI A'CATG_T
Cuts at: 7399 7399
Size: 8500
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2116 2779 2847 3763 4636 4894 1108
Size: 1008 663 68 916 873 258 4714
Fragments arranged by size:
4714 1008 916 873 663 258 68
PfoI T'CCnGG_A
Cuts at: 2805 3702 3923 2805
Size: 897 221 7382
Fragments arranged by size:
7382 897 221
PmeI GTTT'AAAC
Cuts at: 5804 5804
Size: 8500
PmlI CAC'GTG
Cuts at: 3636 4224 3636
Size: 588 7912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5291 5323 5372 5404 6569 6601 8082
Size: 32 1234 32 49 32 1165 32 1481
Cuts at: 8082 8114 4025
Size: 32 4411
Fragments arranged by size:
4411 1481 1234 1165 49 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3462 4936 5131 5257 5553 3462
Size: 1474 195 126 296 6409
Fragments arranged by size:
6409 1474 296 195 126
PshAI GACnn'nnGTC
Cuts at: 1613 4142 1613
Size: 2529 5971
PsiI TTA'TAA
Cuts at: 6070 6070
Size: 8500
PspOMI G'GGCC_C
Cuts at: 3590 4304 4733 5020 3590
Size: 714 429 287 7070
Fragments arranged by size:
7070 714 429 287
PstI C_TGCA'G
Cuts at: 830 2066 2916 3937 5265 5852 830
Size: 1236 850 1021 1328 587 3478
Fragments arranged by size:
3478 1328 1236 1021 850 587
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8500
PvuII CAG'CTG
Cuts at: 2917 3292 3792 2917
Size: 375 500 7625
Fragments arranged by size:
7625 500 375
SacI G_AGCT'C
Cuts at: 5243 5243
Size: 8500
SacII CC_GC'GG
Cuts at: 4561 4561
Size: 8500
SalI G'TCGA_C
Cuts at: 1936 5842 1936
Size: 3906 4594
SanDI GG'GwC_CC
Cuts at: 3462 4936 5131 3462
Size: 1474 195 6831
Fragments arranged by size:
6831 1474 195
SapI GCTCTTCn'nnn_
Cuts at: 2256 2756 2256
Size: 500 8000
SbfI CC_TGCA'GG
Cuts at: 5852 5852
Size: 8500
ScaI AGT'ACT
Cuts at: 3728 6748 8460 3728
Size: 3020 1712 3768
Fragments arranged by size:
3768 3020 1712
SexAI A'CCwGG_T
Cuts at: 2775 2775
Size: 8500
SfcI C'TryA_G
Cuts at: 826 1045 2062 2912 3769 3787 3933 4684 5113
Size: 219 1017 850 857 18 146 751 429
Cuts at: 5113 5261 5848 6983 7664 7855 826
Size: 148 587 1135 681 191 1471
Fragments arranged by size:
1471 1135 1017 857 850 751 681 587
429 219 191 148 146 18
SfoI GGC'GCC
Cuts at: 2153 3184 3746 3996 4286 2153
Size: 1031 562 250 290 6367
Fragments arranged by size:
6367 1031 562 290 250
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8500
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8500
SmaI CCC'GGG
Cuts at: 1872 4114 5827 1872
Size: 2242 1713 4545
Fragments arranged by size:
4545 2242 1713
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 4052 4498 6604 7505 7767
Size: 197 926 243 1866 446 2106 901 262
Cuts at: 7767 820
Size: 1553
Fragments arranged by size:
2106 1866 1553 926 901 446 262 243
197
SnaBI TAC'GTA
Cuts at: 493 5797 6291 493
Size: 5304 494 2702
Fragments arranged by size:
5304 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8500
SphI G_CATG'C
Cuts at: 1132 2993 5858 1132
Size: 1861 2865 3774
Fragments arranged by size:
3774 2865 1861
SspI AAT'ATT
Cuts at: 5 52 6424 5
Size: 47 6372 2081
Fragments arranged by size:
6372 2081 47
StuI AGG'CCT
Cuts at: 1517 3371 3644 4712 5582 1517
Size: 1854 273 1068 870 4435
Fragments arranged by size:
4435 1854 1068 870 273
StyI C'CwwG_G
Cuts at: 513 1065 2022 2256 5136 5181 5939 513
Size: 552 957 234 2880 45 758 3074
Fragments arranged by size:
3074 2880 957 758 552 234 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2258 4979 5513 5536 6527 6544 6697
Size: 1255 678 2721 534 23 991 17 153
Cuts at: 6697 6882 7327 325
Size: 185 445 1498
Fragments arranged by size:
2721 1498 1255 991 678 534 445 185
153 23 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3726 5547 5706
Size: 274 80 33 51 1443 1749 1821 159
Cuts at: 5706 6746 8458 96
Size: 1040 1712 138
Fragments arranged by size:
1821 1749 1712 1443 1040 274 159 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2097 2129 2285 2317 3163 3195 3369 3401 4554
Size: 32 156 32 846 32 174 32 1153
Cuts at: 4554 4586 5132 5164 8460 8492 2097
Size: 32 546 32 3296 32 2105
Fragments arranged by size:
3296 2105 1153 846 546 174 156 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2882 1316
Size: 1566 6934
XbaI T'CTAG_A
Cuts at: 5836 5836
Size: 8500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2377 2773 3114 3217 3463 3808 2263
Size: 114 396 341 103 246 345 6955
Fragments arranged by size:
6955 396 345 341 246 114 103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8500
XmaI C'CCGG_G
Cuts at: 1870 4112 5825 1870
Size: 2242 1713 4545
Fragments arranged by size:
4545 2242 1713
XmnI GAAnn'nnTTC
Cuts at: 6629 6629
Size: 8500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6308 276
Size: 53 83 186 936 4774 2468
Fragments arranged by size:
4774 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SfiI SrfI SwaI