Restriction map for
pFN21ASDB0185
(Circular) (Six-base) MAPSORT of: pFN21ASDB0185.seq Check: 2082 from: 1 to: 9667
pFN21ASDB0185 9667 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3190 6810 6939 3190
Size: 3620 129 5918
Fragments arranged by size:
5918 3620 129
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7477 278
Size: 53 83 186 936 5941 2468
Fragments arranged by size:
5941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7010 1937
Size: 5073 4594
Acc65I G'GTAC_C
Cuts at: 6988 6988
Size: 9667
AclI AA'CG_TT
Cuts at: 1563 7794 8167 1563
Size: 6231 373 3063
Fragments arranged by size:
6231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3085 3109 3767 3795 3953 4607 4861
Size: 1280 173 24 658 28 158 654 254
Cuts at: 4861 4918 5819 7730 9114 9382 1632
Size: 57 901 1911 1384 268 1917
Fragments arranged by size:
1917 1911 1384 1280 901 658 654 268
254 173 158 57 28 24
AfeI AGC'GCT
Cuts at: 4216 4216
Size: 9667
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9470
AflIII A'CryG_T
Cuts at: 1203 2511 5589 8566 9543 1203
Size: 1308 3078 2977 977 1327
Fragments arranged by size:
3078 2977 1327 1308 977
AgeI A'CCGG_T
Cuts at: 8498 8498
Size: 9667
AhdI GACnn_n'nnGTC
Cuts at: 6469 8396 6469
Size: 1927 7740
AleI CACnn'nnGTG
Cuts at: 1166 2937 3552 3995 1166
Size: 1771 615 443 6838
Fragments arranged by size:
6838 1771 615 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2945 2955 4013 6258 1184
Size: 1761 10 1058 2245 4593
Fragments arranged by size:
4593 2245 1761 1058 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5576 5608 6897 6929 5576
Size: 32 1289 32 8314
Fragments arranged by size:
8314 1289 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2215 2734 2925 3205 3793 4449 4639 4792 5003
Size: 519 191 280 588 656 190 153 211
Cuts at: 5003 5271 7410 8982 2215
Size: 268 2139 1572 2900
Fragments arranged by size:
2900 2139 1572 656 588 519 280 268
211 191 190 153
ApaLI G'TGCA_C
Cuts at: 6589 7724 8880 6589
Size: 1135 1156 7376
Fragments arranged by size:
7376 1156 1135
ApoI r'AATT_y
Cuts at: 1493 3636 6976 7202 7354 9463 1493
Size: 2143 3340 226 152 2109 1697
Fragments arranged by size:
3340 2143 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 4438 4438
Size: 9667
AseI AT'TA_AT
Cuts at: 160 1032 8221 160
Size: 872 7189 1606
Fragments arranged by size:
7189 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9667
AvaI C'yCGr_G
Cuts at: 1870 1943 2194 3377 3925 4766 5749 6528 6992
Size: 73 251 1183 548 841 983 779 464
Cuts at: 6992 1870
Size: 4545
Fragments arranged by size:
4545 1183 983 841 779 548 464 251
73
BamHI G'GATC_C
Cuts at: 2133 6997 7364 2133
Size: 4864 367 4436
Fragments arranged by size:
4864 4436 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3554 4112 4642 4914 6028
Size: 325 217 627 1767 558 530 272 1114
Cuts at: 6028 6498 6988 8443 618
Size: 470 490 1455 1842
Fragments arranged by size:
1842 1767 1455 1114 627 558 530 490
470 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2457 2919 3921 4507 4551 4698 4839 6006
Size: 1066 462 1002 586 44 147 141 1167
Cuts at: 6006 6529 6986 1391
Size: 523 457 4072
Fragments arranged by size:
4072 1167 1066 1002 586 523 462 457
147 141 44
BbeI G_GCGC'C
Cuts at: 6502 6502
Size: 9667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2416 3259 3372 3569 4167 4345 6119
Size: 971 517 843 113 197 598 178 1774
Cuts at: 6119 6904 928
Size: 785 3691
Fragments arranged by size:
3691 1774 971 843 785 598 517 197
178 113
BbvCI CC'TCA_GC
Cuts at: 3291 4715 4824 6818 3291
Size: 1424 109 1994 6140
Fragments arranged by size:
6140 1994 1424 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4729 4763 5339 5373 6409 6443 7858
Size: 34 2792 34 576 34 1036 34 1415
Cuts at: 7858 7892 1903
Size: 34 3678
Fragments arranged by size:
3678 2792 1415 1036 576 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7559 8769 7559
Size: 1210 8457
BclI T'GATC_A
Cuts at: 1548 2328 1548
Size: 780 8887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4055 4673 5102
Size: 107 122 71 631 605 2383 618 429
Cuts at: 5102 5225 5777 6093 8278 136
Size: 123 552 316 2185 1525
Fragments arranged by size:
2383 2185 1525 631 618 605 552 429
316 123 122 107 71
BglII A'GATC_T
Cuts at: 6894 9662 6894
Size: 2768 6899
BlpI GC'TnA_GC
Cuts at: 2828 2918 6416 7084 2828
Size: 90 3498 668 5411
Fragments arranged by size:
5411 3498 668 90
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2323 3683 3773 4194 4755 6033 6593
Size: 388 145 1360 90 421 561 1278 560
Cuts at: 6593 7728 8884 1790
Size: 1135 1156 2573
Fragments arranged by size:
2573 1360 1278 1156 1135 561 560 421
388 145 90
BmgBI CAC'GTC
Cuts at: 1318 3761 5353 1318
Size: 2443 1592 5632
Fragments arranged by size:
5632 2443 1592
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4459 4554 5310
Size: 416 53 83 399 24 3042 95 756
Cuts at: 5310 7398 8356 442
Size: 2088 958 1753
Fragments arranged by size:
3042 2088 1753 958 756 416 399 95
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9667
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3940 3940
Size: 9667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2956 3830 3928 4226 4307 4880 4980 5761
Size: 1717 874 98 298 81 573 100 781
Cuts at: 5761 6380 6740 6866 8327 1239
Size: 619 360 126 1461 2579
Fragments arranged by size:
2579 1717 1461 874 781 619 573 360
298 126 100 98 81
Bpu10I CC'TnA_GC
Cuts at: 2033 3291 3842 4715 4824 6309 6818 6917 2033
Size: 1258 551 873 109 1485 509 99 4783
Fragments arranged by size:
4783 1485 1258 873 551 509 109 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4082 6635 7792 8657 8955 4082
Size: 2553 1157 865 298 4794
Fragments arranged by size:
4794 2553 1157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3147 3492 3596 4032 4277 5074 5816
Size: 617 1648 345 104 436 245 797 742
Cuts at: 5816 8330 882
Size: 2514 2219
Fragments arranged by size:
2514 2219 1648 797 742 617 436 345
245 104
BsaAI yAC'GTr
Cuts at: 493 1204 4189 5260 5590 6964 7458 493
Size: 711 2985 1071 330 1374 494 2702
Fragments arranged by size:
2985 2702 1374 1071 711 494 330
BsaBI GATnn'nnATC
Cuts at: 2138 3440 9661 2138
Size: 1302 6221 2144
Fragments arranged by size:
6221 2144 1302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3936 6499 7474 7856
Size: 53 83 186 936 2403 2563 975 382
Cuts at: 7856 8491 275
Size: 635 1451
Fragments arranged by size:
2563 2403 1451 975 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3660 5865 8100 8498 8772 8919 9330 3660
Size: 2205 2235 398 274 147 411 3997
Fragments arranged by size:
3997 2235 2205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2221 2251 3803 3833 3926
Size: 30 165 30 818 30 1552 30 93
Cuts at: 3926 3956 4058 4088 5244 5274 5285 5315 5657
Size: 30 102 30 1156 30 11 30 342
Cuts at: 5657 5687 6038 6068 6866 6896 1178
Size: 30 351 30 798 30 3949
Fragments arranged by size:
3949 1552 1156 818 798 351 342 165
102 93 30 30 30 30 30 30
30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2960 4529 4532 4678 4877 5665
Size: 853 3 918 1569 3 146 199 788
Cuts at: 5665 5884 6158 6587 6737 6965 1186
Size: 219 274 429 150 228 3888
Fragments arranged by size:
3888 1569 918 853 788 429 274 228
219 199 150 146 3 3
BseYI C'CCAG_C
Cuts at: 3205 3541 3741 4033 4170 4217 4306 4776 4780
Size: 336 200 292 137 47 89 470 4
Cuts at: 4780 5082 5415 5548 5960 6091 6642 6822 8870
Size: 302 333 133 412 131 551 180 2048
Cuts at: 8870 9366 3205
Size: 496 3506
Fragments arranged by size:
3506 2048 551 496 470 412 336 333
302 292 200 180 137 133 131 89
47 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 2891 2915 3779 4541 5243 5258
Size: 573 948 207 24 864 762 702 15
Cuts at: 5258 5653 6086 6632 6713 1163
Size: 395 433 546 81 4117
Fragments arranged by size:
4117 948 864 762 702 573 546 433
395 207 81 24 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3492 4959 5761 7117 7878 8906
Size: 275 186 1491 1467 802 1356 761 1028
Cuts at: 8906 1540
Size: 2301
Fragments arranged by size:
2301 1491 1467 1356 1028 802 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2034 2433 3063 3234 3726 4256 4698 4851 6006
Size: 399 630 171 492 530 442 153 1155
Cuts at: 6006 6422 6593 6780 7728 7813 8884 2034
Size: 416 171 187 948 85 1071 2817
Fragments arranged by size:
2817 1155 1071 948 630 530 492 442
416 399 187 171 171 153 85
BsmI GAATG_Cn'
Cuts at: 5174 7178 7271 5174
Size: 2004 93 7570
Fragments arranged by size:
7570 2004 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5648 5648
Size: 9667
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2178 2323 2433 2457 2919 3063
Size: 399 244 144 145 110 24 462 144
Cuts at: 3063 3234 3683 3726 3773 3921 4194 4256 4507
Size: 171 449 43 47 148 273 62 251
Cuts at: 4507 4551 4698 4755 4839 4851 6006 6033 6422
Size: 44 147 57 84 12 1155 27 389
Cuts at: 6422 6529 6593 6780 6986 7728 7813 8884 1391
Size: 107 64 187 206 742 85 1071 2174
Fragments arranged by size:
2174 1155 1071 742 462 449 399 389
273 251 244 206 187 171 148 147
145 144 144 110 107 85 84 64
62 57 47 44 43 27 24 12
BspEI T'CCGG_A
Cuts at: 3660 5865 9330 3660
Size: 2205 3465 3997
Fragments arranged by size:
3997 3465 2205
BspHI T'CATG_A
Cuts at: 2988 7556 9286 2988
Size: 4568 1730 3369
Fragments arranged by size:
4568 3369 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3190 4038 4661 5166 5838 6810 6939 7022
Size: 2346 848 623 505 672 972 129 83
Cuts at: 7022 844
Size: 3489
Fragments arranged by size:
3489 2346 972 848 672 623 505 129
83
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 4912 7554 1394
Size: 429 2023 1066 2642 3507
Fragments arranged by size:
3507 2642 2023 1066 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2117 2853 4311 5145 8162 8336 65
Size: 1183 869 736 1458 834 3017 174 1396
Fragments arranged by size:
3017 1458 1396 1183 869 834 736 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4115 5760 6422 8311 8498 1700
Size: 110 2305 1645 662 1889 187 2869
Fragments arranged by size:
2869 2305 1889 1645 662 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9667
BssHII G'CGCG_C
Cuts at: 1925 4438 1925
Size: 2513 7154
BssSI C'ACGA_G
Cuts at: 2789 4430 5479 6522 7727 8739 2789
Size: 1641 1049 1043 1205 1012 3717
Fragments arranged by size:
3717 1641 1205 1049 1043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3793 5856 6028 794
Size: 2999 2063 172 4433
Fragments arranged by size:
4433 2999 2063 172
BstBI TT'CG_AA
Cuts at: 2007 8471 2007
Size: 6464 3203
BstEII G'GTnAC_C
Cuts at: 1184 2740 3244 4853 5530 1184
Size: 1556 504 1609 677 5321
Fragments arranged by size:
5321 1609 1556 677 504
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 5008 5581 6379 6481 2410
Size: 2598 573 798 102 5596
Fragments arranged by size:
5596 2598 798 573 102
BstYI r'GATC_y
Cuts at: 1972 2133 3657 3961 6894 6997 7364 7749 7766
Size: 161 1524 304 2933 103 367 385 17
Cuts at: 7766 9218 9304 9316 9662 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2933 1977 1524 1452 385 367 346 304
161 103 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5785 5916 5785
Size: 131 9536
BtgI C'CryG_G
Cuts at: 513 1065 2022 2418 4341 4479 5169 5775 6042
Size: 552 957 396 1923 138 690 606 267
Cuts at: 6042 6489 6516 513
Size: 447 27 3664
Fragments arranged by size:
3664 1923 957 690 606 552 447 396
267 138 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2758 2959 3862 3871 4030 5497
Size: 683 56 1532 201 903 9 159 1467
Cuts at: 5497 6412 487
Size: 915 3742
Fragments arranged by size:
3742 1532 1467 915 903 683 201 159
56 9
BtsI GCAGTG_nn'
Cuts at: 4079 4147 4558 4814 5001 5469 7183 7977 7997
Size: 68 411 256 187 468 1714 794 20
Cuts at: 7997 4079
Size: 5749
Fragments arranged by size:
5749 1714 794 468 411 256 187 68
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4453 4488 529
Size: 35 3889 35 5708
Fragments arranged by size:
5708 3889 35 35
DraI TTT'AAA
Cuts at: 6971 6971
Size: 9667
DraIII CAC_nnn'GTG
Cuts at: 4189 4189
Size: 9667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8674 809
Size: 507 7358 1802
Fragments arranged by size:
7358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 2658 2793
Size: 54 1425 50 275 18 582 246 135
Cuts at: 2793 3489 4056 4674 5010 5181 5262 5374 5466
Size: 696 567 618 336 171 81 112 92
Cuts at: 5466 5758 5778 6039 6600 7114 8003 9632 8
Size: 292 20 261 561 514 889 1629 43
Fragments arranged by size:
1629 1425 889 696 618 582 567 561
514 336 292 275 261 246 171 135
112 92 81 54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3489 5758 7114 1537
Size: 275 1677 2269 1356 4090
Fragments arranged by size:
4090 2269 1677 1356 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2114 2969 3005 3659 4703 5168 5816 7597
Size: 761 855 36 654 1044 465 648 1781
Cuts at: 7597 1353
Size: 3423
Fragments arranged by size:
3423 1781 1044 855 761 654 648 465
36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2229 2640 5890 8254 8628 8774
Size: 1064 138 640 411 3250 2364 374 146
Cuts at: 8774 387
Size: 1280
Fragments arranged by size:
3250 2364 1280 1064 640 411 374 146
138
EcoICRI GAG'CTC
Cuts at: 4696 6004 4696
Size: 1308 8359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2912 2956 3085 3109 3767 3795 3830
Size: 393 1280 44 129 24 658 28 35
Cuts at: 3830 3928 3953 4226 4307 4607 4861 4880 4918
Size: 98 25 273 81 300 254 19 38
Cuts at: 4918 4980 5761 5819 6380 6740 6866 7730 8327
Size: 62 781 58 561 360 126 864 597
Cuts at: 8327 9114 9382 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1280 864 787 781 658 597 561
393 360 300 273 268 254 129 126
98 81 62 58 44 38 35 28
25 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2241 3455 3518 4511 4589 5479 2241
Size: 1214 63 993 78 890 6429
Fragments arranged by size:
6429 1214 993 890 78 63
EcoO109I rG'GnC_Cy
Cuts at: 2086 3421 3751 4083 4636 4770 5293 5943 6324
Size: 1335 330 332 553 134 523 650 381
Cuts at: 6324 6349 6583 2086
Size: 25 234 5170
Fragments arranged by size:
5170 1335 650 553 523 381 332 330
234 134 25
EcoRI G'AATT_C
Cuts at: 6976 7354 6976
Size: 378 9289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4373 4373
Size: 9667
FspI TGC'GCA
Cuts at: 2315 2906 5543 8173 2315
Size: 591 2637 2630 3809
Fragments arranged by size:
3809 2637 2630 591
FspAI rTGC'GCAy
Cuts at: 2906 5543 2906
Size: 2637 7030
HaeII r_GCGC'y
Cuts at: 1678 2048 4218 5097 5748 6316 6502 6719 8814
Size: 370 2170 879 651 568 186 217 2095
Cuts at: 8814 1678
Size: 2531
Fragments arranged by size:
2531 2170 2095 879 651 568 370 217
186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 3926 3958 4029
Size: 32 135 32 809 32 1513 32 71
Cuts at: 4029 4061 4494 4526 5285 5317 6038 6070 6455
Size: 32 433 32 759 32 721 32 385
Cuts at: 6455 6487 6866 6898 8308 8340 8382 8414 1373
Size: 32 379 32 1410 32 42 32 2626
Fragments arranged by size:
2626 1513 1410 809 759 721 433 385
379 135 71 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3172 4963 7011 7257 669
Size: 988 281 1234 1791 2048 246 3079
Fragments arranged by size:
3079 2048 1791 1234 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9667
HpaI GTT'AAC
Cuts at: 7257 7257
Size: 9667
KasI G'GCGC_C
Cuts at: 6498 6498
Size: 9667
KpnI G_GTAC'C
Cuts at: 6992 6992
Size: 9667
MfeI C'AATT_G
Cuts at: 7266 7266
Size: 9667
MluI A'CGCG_T
Cuts at: 9543 9543
Size: 9667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 4515 5097 6181 8781 8965 1305
Size: 840 2370 582 1084 2600 184 2007
Fragments arranged by size:
2600 2370 2007 1084 840 582 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2795 4058 4676 5183 5264
Size: 54 1425 343 963 1263 618 507 81
Cuts at: 5264 6602 10
Size: 1338 3075
Fragments arranged by size:
3075 1425 1338 1263 963 618 507 343
81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2339 2703 2906 2937 3552 3995
Size: 648 861 312 364 203 31 615 443
Cuts at: 3995 4340 5537 5900 6479 6608 7625 7984 8143
Size: 345 1197 363 579 129 1017 359 159
Cuts at: 8143 518
Size: 2042
Fragments arranged by size:
2042 1197 1017 861 648 615 579 443
364 363 359 345 312 203 159 129
31
MspA1I CmG'CkG
Cuts at: 1606 2041 2464 2722 2809 2925 3627 3745 3973
Size: 435 423 258 87 116 702 118 228
Cuts at: 3973 4174 4529 4605 4780 4792 5552 6025 6217
Size: 201 355 76 175 12 760 473 192
Cuts at: 6217 6669 6822 7083 7760 8908 9153 1606
Size: 452 153 261 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 702 677 473 452 435
423 355 261 258 245 228 201 192
175 153 118 116 87 76 12
NaeI GCC'GGC
Cuts at: 4117 4117
Size: 9667
NarI GG'CG_CC
Cuts at: 6499 6499
Size: 9667
NcoI C'CATG_G
Cuts at: 513 1065 2022 4341 4479 5169 5775 513
Size: 552 957 2319 138 690 606 4405
Fragments arranged by size:
4405 2319 957 690 606 552 138
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9667
NgoMIV G'CCGG_C
Cuts at: 4115 4115
Size: 9667
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9667
NotI GC'GGCC_GC
Cuts at: 7114 7114
Size: 9667
NspI r_CATG'y
Cuts at: 1132 1229 2338 2515 2672 2905 5237 6371 7025
Size: 97 1109 177 157 233 2332 1134 654
Cuts at: 7025 8570 1132
Size: 1545 2229
Fragments arranged by size:
2332 2229 1545 1134 1109 654 233 177
157 97
PasI CC'CwG_GG
Cuts at: 1348 3272 1348
Size: 1924 7743
PciI A'CATG_T
Cuts at: 2511 8566 2511
Size: 6055 3612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3649 4015 4449 5271 5617 6568 6787 1108
Size: 2541 366 434 822 346 951 219 3988
Fragments arranged by size:
3988 2541 951 822 434 366 346 219
PfoI T'CCnGG_A
Cuts at: 2100 2208 2643 3944 6459 6765 2100
Size: 108 435 1301 2515 306 5002
Fragments arranged by size:
5002 2515 1301 435 306 108
PmeI GTTT'AAAC
Cuts at: 6971 6971
Size: 9667
PmlI CAC'GTG
Cuts at: 4189 5260 5590 4189
Size: 1071 330 8266
Fragments arranged by size:
8266 1071 330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7736 7768 9249 9281 7736
Size: 32 1481 32 8122
Fragments arranged by size:
8122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 3421 4083 4636 4770 5293 5943 2086
Size: 1335 662 553 134 523 650 5810
Fragments arranged by size:
5810 1335 662 650 553 523 134
PshAI GACnn'nnGTC
Cuts at: 1613 4655 1613
Size: 3042 6625
PsiI TTA'TAA
Cuts at: 7237 7237
Size: 9667
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 9635
PstI C_TGCA'G
Cuts at: 830 2076 4074 4422 4821 5103 6936 7019 830
Size: 1246 1998 348 399 282 1833 83 3478
Fragments arranged by size:
3478 1998 1833 1246 399 348 282 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9667
PvuII CAG'CTG
Cuts at: 2041 2722 2809 2925 3745 4174 4605 4780 4792
Size: 681 87 116 820 429 431 175 12
Cuts at: 4792 5552 6822 2041
Size: 760 1270 4886
Fragments arranged by size:
4886 1270 820 760 681 431 429 175
116 87 12
SacI G_AGCT'C
Cuts at: 4698 6006 4698
Size: 1308 8359
SalI G'TCGA_C
Cuts at: 1936 7009 1936
Size: 5073 4594
SapI GCTCTTCn'nnn_
Cuts at: 3005 3659 4703 5168 5816 3005
Size: 654 1044 465 648 6856
Fragments arranged by size:
6856 1044 654 648 465
SbfI CC_TGCA'GG
Cuts at: 5103 7019 5103
Size: 1916 7751
ScaI AGT'ACT
Cuts at: 3401 7915 9627 3401
Size: 4514 1712 3441
Fragments arranged by size:
4514 3441 1712
SexAI A'CCwGG_T
Cuts at: 3248 3423 3248
Size: 175 9492
SfcI C'TryA_G
Cuts at: 826 1045 2072 4070 4418 4817 5099 6932 7015
Size: 219 1027 1998 348 399 282 1833 83
Cuts at: 7015 8150 8831 9022 826
Size: 1135 681 191 1471
Fragments arranged by size:
1998 1833 1471 1135 1027 681 399 348
282 219 191 83
SfiI GGCCn_nnn'nGGCC
Cuts at: 4673 5225 5777 4673
Size: 552 552 8563
Fragments arranged by size:
8563 552 552
SfoI GGC'GCC
Cuts at: 6500 6500
Size: 9667
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9667
SmaI CCC'GGG
Cuts at: 1872 2196 4768 5751 6994 1872
Size: 324 2572 983 1243 4545
Fragments arranged by size:
4545 2572 1243 983 324
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4097 6650 7771 8672 8934 820
Size: 197 926 2154 2553 1121 901 262 1553
Fragments arranged by size:
2553 2154 1553 1121 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6964 7458 493
Size: 6471 494 2702
Fragments arranged by size:
6471 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9667
SphI G_CATG'C
Cuts at: 1132 2672 7025 1132
Size: 1540 4353 3774
Fragments arranged by size:
4353 3774 1540
SspI AAT'ATT
Cuts at: 5 52 7591 5
Size: 47 7539 2081
Fragments arranged by size:
7539 2081 47
StuI AGG'CCT
Cuts at: 1517 5345 6734 1517
Size: 3828 1389 4450
Fragments arranged by size:
4450 3828 1389
StyI C'CwwG_G
Cuts at: 513 1065 2022 2600 3338 3456 3897 4059 4341
Size: 552 957 578 738 118 441 162 282
Cuts at: 4341 4479 4725 5109 5169 5775 6009 6687 6781
Size: 138 246 384 60 606 234 678 94
Cuts at: 6781 6855 7106 513
Size: 74 251 3074
Fragments arranged by size:
3074 957 738 678 606 578 552 441
384 282 251 246 234 162 138 118
94 74 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4022 7694 7711 7864 8049 8494 325
Size: 1255 2442 3672 17 153 185 445 1498
Fragments arranged by size:
3672 2442 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2898 3399 7913
Size: 274 80 33 51 1443 921 501 4514
Cuts at: 7913 9625 96
Size: 1712 138
Fragments arranged by size:
4514 1712 1443 921 501 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2221 2253 2552 2584 3803 3835 4058 4090 5244
Size: 32 299 32 1219 32 223 32 1154
Cuts at: 5244 5276 6379 6411 9627 9659 2221
Size: 32 1103 32 3216 32 2229
Fragments arranged by size:
3216 2229 1219 1154 1103 299 223 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9667
XbaI T'CTAG_A
Cuts at: 7003 7003
Size: 9667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2250 3928 4012 4201 4339 5176 5524 5614 2250
Size: 1678 84 189 138 837 348 90 6303
Fragments arranged by size:
6303 1678 837 348 189 138 90 84
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9667
XmaI C'CCGG_G
Cuts at: 1870 2194 4766 5749 6992 1870
Size: 324 2572 983 1243 4545
Fragments arranged by size:
4545 2572 1243 983 324
XmnI GAAnn'nnTTC
Cuts at: 5138 5450 6833 7796 5138
Size: 312 1383 963 7009
Fragments arranged by size:
7009 1383 963 312
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7475 276
Size: 53 83 186 936 5941 2468
Fragments arranged by size:
5941 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI
NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI
SrfI SwaI