Restriction map for
pFN21ASDB0068
(Circular) (Six-base) MAPSORT of: pFN21ASDB0068.seq Check: 8132 from: 1 to: 7825
pFN21ASDB0068 7825 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3447 3447
Size: 7825
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4437 5635 278
Size: 53 83 186 936 2901 1198 2468
Fragments arranged by size:
2901 2468 1198 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5168 1937
Size: 3231 4594
Acc65I G'GTAC_C
Cuts at: 4698 5146 4698
Size: 448 7377
AclI AA'CG_TT
Cuts at: 1563 5952 6325 1563
Size: 4389 373 3063
Fragments arranged by size:
4389 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4088 4241 4298 4679 5888 7272 7540 1632
Size: 2456 153 57 381 1209 1384 268 1917
Fragments arranged by size:
2456 1917 1384 1209 381 268 153 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7628
AflIII A'CryG_T
Cuts at: 1203 6724 7701 1203
Size: 5521 977 1327
Fragments arranged by size:
5521 1327 977
AgeI A'CCGG_T
Cuts at: 6656 6656
Size: 7825
AhdI GACnn_n'nnGTC
Cuts at: 3523 6554 3523
Size: 3031 4794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2072 4325 1184
Size: 888 2253 4684
Fragments arranged by size:
4684 2253 888
AlwNI CAG_nnn'CTG
Cuts at: 3493 3565 4318 5568 7140 3493
Size: 72 753 1250 1572 4178
Fragments arranged by size:
4178 1572 1250 753 72
ApaLI G'TGCA_C
Cuts at: 2489 4653 5882 7038 2489
Size: 2164 1229 1156 3276
Fragments arranged by size:
3276 2164 1229 1156
ApoI r'AATT_y
Cuts at: 1493 2433 2448 3022 5134 5360 5512 7621 1493
Size: 940 15 574 2112 226 152 2109 1697
Fragments arranged by size:
2112 2109 1697 940 574 226 152 15
AseI AT'TA_AT
Cuts at: 160 1032 6379 160
Size: 872 5347 1606
Fragments arranged by size:
5347 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
AvaI C'yCGr_G
Cuts at: 1870 1943 2361 2897 3254 3399 4386 4443 4747
Size: 73 418 536 357 145 987 57 304
Cuts at: 4747 5150 1870
Size: 403 4545
Fragments arranged by size:
4545 987 536 418 403 357 304 145
73 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4930 4963 4930
Size: 33 7792
BamHI G'GATC_C
Cuts at: 5155 5522 5155
Size: 367 7458
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 2509 3834 3987 4676
Size: 325 217 627 474 248 1325 153 689
Cuts at: 4676 4698 4832 5146 6601 618
Size: 22 134 314 1455 1842
Fragments arranged by size:
1842 1455 1325 689 627 474 325 314
248 217 153 134 22
BanII G_rGCy'C
Cuts at: 1391 3201 3900 4215 4473 4748 4844 5144 1391
Size: 1810 699 315 258 275 96 300 4072
Fragments arranged by size:
4072 1810 699 315 300 275 258 96
BbeI G_GCGC'C
Cuts at: 4680 4680
Size: 7825
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3083 4205 4482 928
Size: 971 1184 1122 277 4271
Fragments arranged by size:
4271 1184 1122 971 277
BbvCI CC'TCA_GC
Cuts at: 2331 2832 3666 5070 2331
Size: 501 834 1404 5086
Fragments arranged by size:
5086 1404 834 501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 6016 6050 1903
Size: 34 2468 34 1577 34 3678
Fragments arranged by size:
3678 2468 1577 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5717 6927 5717
Size: 1210 6615
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7825
BfrBI ATG'CAT
Cuts at: 2216 2216
Size: 7825
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6436 136
Size: 107 122 71 631 605 4764 1525
Fragments arranged by size:
4764 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4579 7820 4579
Size: 3241 4584
BlpI GC'TnA_GC
Cuts at: 3039 3117 3122 3431 5242 3039
Size: 78 5 309 1811 5622
Fragments arranged by size:
5622 1811 309 78 5
Bme1580I G_kGCm'C
Cuts at: 1790 2493 2840 4657 5886 7042 1790
Size: 703 347 1817 1229 1156 2573
Fragments arranged by size:
2573 1817 1229 1156 703 347
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7825
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5556 6514 442
Size: 416 53 83 399 24 4139 958 1753
Fragments arranged by size:
4139 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7825
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3215
Size: 7825
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2769 2870 3131 3515 3629 3839 3866 4019
Size: 1530 101 261 384 114 210 27 153
Cuts at: 4019 4139 4220 4379 4391 4667 6485 1239
Size: 120 81 159 12 276 1818 2579
Fragments arranged by size:
2579 1818 1530 384 276 261 210 159
153 120 114 101 81 27 12
Bpu10I CC'TnA_GC
Cuts at: 2331 2832 3462 3666 5070 2331
Size: 501 630 204 1404 5086
Fragments arranged by size:
5086 1404 630 501 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2181 2423 3341 4033 4346 4541 5950 6815 7113
Size: 242 918 692 313 195 1409 865 298
Cuts at: 7113 2181
Size: 2893
Fragments arranged by size:
2893 1409 918 865 692 313 298 242
195
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 2954 3203 6488 882
Size: 617 525 930 249 3285 2219
Fragments arranged by size:
3285 2219 930 617 525 249
BsaAI yAC'GTr
Cuts at: 493 1204 4568 5122 5616 493
Size: 711 3364 554 494 2702
Fragments arranged by size:
3364 2702 711 554 494
BsaBI GATnn'nnATC
Cuts at: 2342 7819 2342
Size: 5477 2348
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4434 4677 5632 6014
Size: 53 83 186 936 2901 243 955 382
Cuts at: 6014 6649 275
Size: 635 1451
Fragments arranged by size:
2901 1451 955 936 635 382 243 186
83 53
BsaWI w'CCGG_w
Cuts at: 6258 6656 6930 7077 7488 6258
Size: 398 274 147 411 6595
Fragments arranged by size:
6595 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 3132 3162 3602
Size: 30 165 30 966 30 733 30 440
Cuts at: 3602 3632 1178
Size: 30 5371
Fragments arranged by size:
5371 966 733 440 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2570 3191 3221 3272 3290 3293 3417 3512
Size: 1384 621 30 51 18 3 124 95
Cuts at: 3512 3554 3779 3782 3905 4388 1186
Size: 42 225 3 123 483 4623
Fragments arranged by size:
4623 1384 621 483 225 124 123 95
51 42 30 18 3 3
BseYI C'CCAG_C
Cuts at: 3170 4804 4910 7028 7524 3170
Size: 1634 106 2118 496 3471
Fragments arranged by size:
3471 2118 1634 496 106
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2306 4479 1163
Size: 573 570 2173 4509
Fragments arranged by size:
4509 2173 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5275 6036 7064 1540
Size: 275 186 3274 761 1028 2301
Fragments arranged by size:
3274 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2493 3201 3900 4215 4304 4657 4748 5886
Size: 432 708 699 315 89 353 91 1138
Cuts at: 5886 5971 7042 2061
Size: 85 1071 2844
Fragments arranged by size:
2844 1138 1071 708 699 432 353 315
91 89 85
BsmI GAATG_Cn'
Cuts at: 5336 5429 5336
Size: 93 7732
BsmBI CGTCTCn'nnnn_
Cuts at: 3089 3296 3089
Size: 207 7618
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2493 2840 3201 3900 4215 4304
Size: 399 271 432 347 361 699 315 89
Cuts at: 4304 4473 4657 4748 4844 5144 5886 5971 7042
Size: 169 184 91 96 300 742 85 1071
Cuts at: 7042 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 699 432 399 361 347
315 300 271 184 169 96 91 89
85
BspEI T'CCGG_A
Cuts at: 7488 7488
Size: 7825
BspHI T'CATG_A
Cuts at: 5714 7444 5714
Size: 1730 6095
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3447 3478 4905 5058 5180 844
Size: 2603 31 1427 153 122 3489
Fragments arranged by size:
3489 2603 1427 153 122 31
BsrBI CCG'CTC
Cuts at: 1394 1823 3262 3945 5712 1394
Size: 429 1439 683 1767 3507
Fragments arranged by size:
3507 1767 1439 683 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2818 3142 6320 6494 65
Size: 1183 1570 324 3178 174 1396
Fragments arranged by size:
3178 1570 1396 1183 324 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3126 3837 4155 4695 6469 6656 1700
Size: 110 1316 711 318 540 1774 187 2869
Fragments arranged by size:
2869 1774 1316 711 540 318 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7825
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7825
BssSI C'ACGA_G
Cuts at: 2492 4292 5885 6897 2492
Size: 1800 1593 1012 3420
Fragments arranged by size:
3420 1800 1593 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3367 794
Size: 2573 5252
BstBI TT'CG_AA
Cuts at: 2007 6629 2007
Size: 4622 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7825
BstYI r'GATC_y
Cuts at: 1972 3282 3691 3939 4579 5155 5522 5907 5924
Size: 1310 409 248 640 576 367 385 17
Cuts at: 5924 7376 7462 7474 7820 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1310 640 576 409 385 367
346 248 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2290 2290
Size: 7825
BtgI C'CryG_G
Cuts at: 513 1065 2022 2307 2388 513
Size: 552 957 285 81 5950
Fragments arranged by size:
5950 957 552 285 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3232 4032 487
Size: 683 56 2006 800 4280
Fragments arranged by size:
4280 2006 800 683 56
BtsI GCAGTG_nn'
Cuts at: 3009 3050 4960 5341 6135 6155 3009
Size: 41 1910 381 794 20 4679
Fragments arranged by size:
4679 1910 794 381 41 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7825
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7790
DraI TTT'AAA
Cuts at: 5129 5129
Size: 7825
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2138 2482 6832 809
Size: 507 822 344 4350 1802
Fragments arranged by size:
4350 1802 822 507 344
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3591 4026 4795
Size: 54 1425 50 275 18 1761 435 769
Cuts at: 4795 5272 6161 7790 8
Size: 477 889 1629 43
Fragments arranged by size:
1761 1629 1425 889 769 477 435 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5272 1537
Size: 275 3460 4090
Fragments arranged by size:
4090 3460 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3375 3539 3990 4274 4292 4877 5755 1353
Size: 2022 164 451 284 18 585 878 3423
Fragments arranged by size:
3423 2022 878 585 451 284 164 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3275 6412 6786 6932 387
Size: 1064 138 453 1233 3137 374 146 1280
Fragments arranged by size:
3137 1280 1233 1064 453 374 146 138
EcoICRI GAG'CTC
Cuts at: 3199 3898 4213 4746 3199
Size: 699 315 533 6278
Fragments arranged by size:
6278 699 533 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2769 2870 3131 3515 3629 3839 3866
Size: 393 1137 101 261 384 114 210 27
Cuts at: 3866 4019 4088 4139 4220 4241 4298 4379 4391
Size: 153 69 51 81 21 57 81 12
Cuts at: 4391 4667 4679 5888 6485 7272 7540 1239
Size: 276 12 1209 597 787 268 1524
Fragments arranged by size:
1524 1209 1137 787 597 393 384 276
268 261 210 153 114 101 81 81
69 57 51 27 21 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2081 2081
Size: 7825
EcoO109I rG'GnC_Cy
Cuts at: 3490 4357 4642 3490
Size: 867 285 6673
Fragments arranged by size:
6673 867 285
EcoRI G'AATT_C
Cuts at: 5134 5512 5134
Size: 378 7447
EcoRV GAT'ATC
Cuts at: 5029 5029
Size: 7825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2261 4531 2261
Size: 2270 5555
FspI TGC'GCA
Cuts at: 3245 6331 3245
Size: 3086 4739
FspAI rTGC'GCAy
Cuts at: 3245 3245
Size: 7825
HaeII r_GCGC'y
Cuts at: 1678 3166 4680 4696 6972 1678
Size: 1488 1514 16 2276 2531
Fragments arranged by size:
2531 2276 1514 1488 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2951 2983 3215 3247 3274
Size: 32 135 32 1379 32 232 32 27
Cuts at: 3274 3306 3509 3541 3563 3595 3676 3708 3777
Size: 32 203 32 22 32 81 32 69
Cuts at: 3777 3809 3887 3919 4120 4152 6466 6498 6540
Size: 32 78 32 201 32 2314 32 42
Cuts at: 6540 6572 1373
Size: 32 2626
Fragments arranged by size:
2626 2314 1379 232 203 201 135 81
78 69 42 32 32 32 32 32
32 32 32 32 32 32 32 32
27 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 5169 5415 669
Size: 988 281 1095 2136 246 3079
Fragments arranged by size:
3079 2136 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4253 5084 748
Size: 3505 831 3489
Fragments arranged by size:
3505 3489 831
HpaI GTT'AAC
Cuts at: 5415 5415
Size: 7825
KasI G'GCGC_C
Cuts at: 4676 4676
Size: 7825
KpnI G_GTAC'C
Cuts at: 4702 5150 4702
Size: 448 7377
MfeI C'AATT_G
Cuts at: 5424 5424
Size: 7825
MluI A'CGCG_T
Cuts at: 7701 7701
Size: 7825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6939 7123 1305
Size: 5634 184 2007
Fragments arranged by size:
5634 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3593 4028 4797 10
Size: 54 1425 343 1761 435 769 3038
Fragments arranged by size:
3038 1761 1425 769 435 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 2217 2612 2975 3239 5783 6142
Size: 648 888 163 395 363 264 2544 359
Cuts at: 6142 6301 518
Size: 159 2042
Fragments arranged by size:
2544 2042 888 648 395 363 359 264
163 159
MspA1I CmG'CkG
Cuts at: 1606 2335 2989 3424 3625 3844 4162 4561 5241
Size: 729 654 435 201 219 318 399 680
Cuts at: 5241 5918 7066 7311 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 729 680 677 654 435 399
318 245 219 201
NaeI GCC'GGC
Cuts at: 3839 3839
Size: 7825
NarI GG'CG_CC
Cuts at: 4677 4677
Size: 7825
NcoI C'CATG_G
Cuts at: 513 1065 2022 2307 2388 513
Size: 552 957 285 81 5950
Fragments arranged by size:
5950 957 552 285 81
NdeI CA'TA_TG
Cuts at: 387 2161 387
Size: 1774 6051
NgoMIV G'CCGG_C
Cuts at: 3837 3837
Size: 7825
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7825
NotI GC'GGCC_GC
Cuts at: 5272 5272
Size: 7825
NsiI A_TGCA'T
Cuts at: 2218 2218
Size: 7825
NspI r_CATG'y
Cuts at: 1132 1229 2220 3964 5183 6728 1132
Size: 97 991 1744 1219 1545 2229
Fragments arranged by size:
2229 1744 1545 1219 991 97
PasI CC'CwG_GG
Cuts at: 1348 3187 1348
Size: 1839 5986
PciI A'CATG_T
Cuts at: 6724 6724
Size: 7825
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3535 3565 1108
Size: 2427 30 5368
Fragments arranged by size:
5368 2427 30
PfoI T'CCnGG_A
Cuts at: 2886 3492 4173 2886
Size: 606 681 6538
Fragments arranged by size:
6538 681 606
PmeI GTTT'AAAC
Cuts at: 5129 5129
Size: 7825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 3755 3787 5894 5926 7407 7439 2999
Size: 32 724 32 2107 32 1481 32 3385
Fragments arranged by size:
3385 2107 1481 724 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3490 4357 3490
Size: 867 6958
PshAI GACnn'nnGTC
Cuts at: 1613 4445 1613
Size: 2832 4993
PsiI TTA'TAA
Cuts at: 2949 5395 2949
Size: 2446 5379
PspXI vC'TCGA_Gb
Cuts at: 4443 4443
Size: 7825
PstI C_TGCA'G
Cuts at: 830 3489 4128 4314 4902 5177 830
Size: 2659 639 186 588 275 3478
Fragments arranged by size:
3478 2659 639 588 275 186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7825
PvuII CAG'CTG
Cuts at: 2335 3844 4162 4561 2335
Size: 1509 318 399 5599
Fragments arranged by size:
5599 1509 399 318
SacI G_AGCT'C
Cuts at: 3201 3900 4215 4748 3201
Size: 699 315 533 6278
Fragments arranged by size:
6278 699 533 315
SalI G'TCGA_C
Cuts at: 1936 5167 1936
Size: 3231 4594
SapI GCTCTTCn'nnn_
Cuts at: 3375 3539 4274 4292 3375
Size: 164 735 18 6908
Fragments arranged by size:
6908 735 164 18
SbfI CC_TGCA'GG
Cuts at: 4902 5177 4902
Size: 275 7550
ScaI AGT'ACT
Cuts at: 6073 7785 6073
Size: 1712 6113
SfcI C'TryA_G
Cuts at: 826 1045 3485 3629 4124 4310 4898 5173 6308
Size: 219 2440 144 495 186 588 275 1135
Cuts at: 6308 6989 7180 826
Size: 681 191 1471
Fragments arranged by size:
2440 1471 1135 681 588 495 275 219
191 186 144
SfoI GGC'GCC
Cuts at: 4678 4678
Size: 7825
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7825
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7825
SmaI CCC'GGG
Cuts at: 1872 4388 5152 1872
Size: 2516 764 4545
Fragments arranged by size:
4545 2516 764
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2196 2402 3356 4012 4361 4443
Size: 197 926 253 206 954 656 349 82
Cuts at: 4443 4520 5929 6830 7092 820
Size: 77 1409 901 262 1553
Fragments arranged by size:
1553 1409 954 926 901 656 349 262
253 206 197 82 77
SnaBI TAC'GTA
Cuts at: 493 5122 5616 493
Size: 4629 494 2702
Fragments arranged by size:
4629 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7825
SphI G_CATG'C
Cuts at: 1132 5183 1132
Size: 4051 3774
SspI AAT'ATT
Cuts at: 5 52 5749 5
Size: 47 5697 2081
Fragments arranged by size:
5697 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7825
StyI C'CwwG_G
Cuts at: 513 1065 2022 2125 2307 2388 4668 4980 5264
Size: 552 957 103 182 81 2280 312 284
Cuts at: 5264 513
Size: 3074
Fragments arranged by size:
3074 2280 957 552 312 284 182 103
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4831 4924 5852 5869 6022 6207 6652
Size: 1255 3251 93 928 17 153 185 445
Cuts at: 6652 325
Size: 1498
Fragments arranged by size:
3251 1498 1255 928 445 185 153 93
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2528 4929 6071
Size: 274 80 33 51 1443 551 2401 1142
Cuts at: 6071 7783 96
Size: 1712 138
Fragments arranged by size:
2401 1712 1443 1142 551 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 3132 3164 7785 7817 2369
Size: 32 731 32 4621 32 2377
Fragments arranged by size:
4621 2377 731 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2138 2965 1316
Size: 822 827 6176
Fragments arranged by size:
6176 827 822
XbaI T'CTAG_A
Cuts at: 5161 5161
Size: 7825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3604 3604
Size: 7825
XhoI C'TCGA_G
Cuts at: 1943 4443 1943
Size: 2500 5325
XmaI C'CCGG_G
Cuts at: 1870 4386 5150 1870
Size: 2516 764 4545
Fragments arranged by size:
4545 2516 764
XmnI GAAnn'nnTTC
Cuts at: 5954 5954
Size: 7825
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4435 5633 276
Size: 53 83 186 936 2901 1198 2468
Fragments arranged by size:
2901 2468 1198 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BsiWI BstXI BstZ17I
DraIII FseI NruI PacI PmlI PspOMI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI