Restriction map for
pFN21ASDB0062
(Circular) (Six-base) MAPSORT of: pFN21ASDB0062.seq Check: 2425 from: 1 to: 6739
pFN21ASDB0062 6739 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4549 278
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4082 1937
Size: 2145 4594
Acc65I G'GTAC_C
Cuts at: 4060 4060
Size: 6739
AclI AA'CG_TT
Cuts at: 1563 4866 5239 1563
Size: 3303 373 3063
Fragments arranged by size:
3303 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3163 3776 4802 6186 6454 1632
Size: 1531 613 1026 1384 268 1917
Fragments arranged by size:
1917 1531 1384 1026 613 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6542
AflIII A'CryG_T
Cuts at: 1203 5638 6615 1203
Size: 4435 977 1327
Fragments arranged by size:
4435 1327 977
AgeI A'CCGG_T
Cuts at: 5570 5570
Size: 6739
AhdI GACnn_n'nnGTC
Cuts at: 5468 5468
Size: 6739
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6739
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2313 1184
Size: 1129 5610
AlwNI CAG_nnn'CTG
Cuts at: 3080 4482 6054 3080
Size: 1402 1572 3765
Fragments arranged by size:
3765 1572 1402
ApaLI G'TGCA_C
Cuts at: 4796 5952 4796
Size: 1156 5583
ApoI r'AATT_y
Cuts at: 1493 2227 2814 2879 3195 3404 4048 4274 4426
Size: 734 587 65 316 209 644 226 152
Cuts at: 4426 6535 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 734 644 587 316 226 209
152 65
AseI AT'TA_AT
Cuts at: 160 1032 2390 2475 5293 160
Size: 872 1358 85 2818 1606
Fragments arranged by size:
2818 1606 1358 872 85
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
AvaI C'yCGr_G
Cuts at: 1870 1943 3513 4064 1870
Size: 73 1570 551 4545
Fragments arranged by size:
4545 1570 551 73
BamHI G'GATC_C
Cuts at: 4069 4436 4069
Size: 367 6372
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2860 4060 5515 618
Size: 325 217 627 1073 1200 1455 1842
Fragments arranged by size:
1842 1455 1200 1073 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4058 1391
Size: 2667 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4017 928
Size: 971 2118 3650
Fragments arranged by size:
3650 2118 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4930 4964 1903
Size: 34 2993 34 3678
Fragments arranged by size:
3678 2993 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3268 4631 5841 3268
Size: 1363 1210 4166
Fragments arranged by size:
4166 1363 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6739
BfrBI ATG'CAT
Cuts at: 3499 3499
Size: 6739
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5350 136
Size: 107 122 71 631 605 3678 1525
Fragments arranged by size:
3678 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6734 6734
Size: 6739
BlpI GC'TnA_GC
Cuts at: 4156 4156
Size: 6739
Bme1580I G_kGCm'C
Cuts at: 1790 4800 5956 1790
Size: 3010 1156 2573
Fragments arranged by size:
3010 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6739
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2050 4470 5428
Size: 416 53 83 399 24 633 2420 958
Cuts at: 5428 442
Size: 1753
Fragments arranged by size:
2420 1753 958 633 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3957 1056
Size: 2901 3838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2993 3345 5399 1239
Size: 1754 352 2054 2579
Fragments arranged by size:
2579 2054 1754 352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2107 2331 3873 4864 5729 6027 2107
Size: 224 1542 991 865 298 2819
Fragments arranged by size:
2819 1542 991 865 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3918 5402 882
Size: 617 2419 1484 2219
Fragments arranged by size:
2419 2219 1484 617
BsaAI yAC'GTr
Cuts at: 493 1204 4036 4530 493
Size: 711 2832 494 2702
Fragments arranged by size:
2832 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 2787 6733 2787
Size: 3946 2793
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 4546 4928 5563
Size: 53 83 186 936 549 2464 382 635
Cuts at: 5563 275
Size: 1451
Fragments arranged by size:
2464 1451 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5172 5570 5844 5991 6402 5172
Size: 398 274 147 411 5509
Fragments arranged by size:
5509 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2386 2416 1178
Size: 30 165 30 983 30 5501
Fragments arranged by size:
5501 983 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3311 3552 1186
Size: 2125 241 4373
Fragments arranged by size:
4373 2125 241
BseYI C'CCAG_C
Cuts at: 3840 3963 5942 6438 3840
Size: 123 1979 496 4141
Fragments arranged by size:
4141 1979 496 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6166
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4189 4950 5978 1540
Size: 275 186 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4800 4885 5956 4800
Size: 85 1071 5583
Fragments arranged by size:
5583 1071 85
BsmI GAATG_Cn'
Cuts at: 3499 4250 4343 3499
Size: 751 93 5895
Fragments arranged by size:
5895 751 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2089 2089
Size: 6739
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4058 4800 4885 5956 1391
Size: 399 2268 742 85 1071 2174
Fragments arranged by size:
2268 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6402 6402
Size: 6739
BspHI T'CATG_A
Cuts at: 4628 6358 4628
Size: 1730 5009
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4094 844
Size: 3250 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4626 1394
Size: 429 2803 3507
Fragments arranged by size:
3507 2803 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3526 5234 5408 65
Size: 1183 2278 1708 174 1396
Fragments arranged by size:
2278 1708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5383 5570 1700
Size: 110 3573 187 2869
Fragments arranged by size:
3573 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6739
BssSI C'ACGA_G
Cuts at: 4799 5811 4799
Size: 1012 5727
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6739
BstBI TT'CG_AA
Cuts at: 2007 5543 2007
Size: 3536 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6739
BstXI CCAn_nnnn'nTGG
Cuts at: 3149 3740 3848 3149
Size: 591 108 6040
Fragments arranged by size:
6040 591 108
BstYI r'GATC_y
Cuts at: 1972 2378 4069 4436 4821 4838 6290 6376 6388
Size: 406 1691 367 385 17 1452 86 12
Cuts at: 6388 6734 1972
Size: 346 1977
Fragments arranged by size:
1977 1691 1452 406 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3294 3294
Size: 6739
BtgI C'CryG_G
Cuts at: 513 1065 2258 513
Size: 552 1193 4994
Fragments arranged by size:
4994 1193 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6000
Fragments arranged by size:
6000 683 56
BtsI GCAGTG_nn'
Cuts at: 4255 5049 5069 4255
Size: 794 20 5925
Fragments arranged by size:
5925 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6739
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6704
DraI TTT'AAA
Cuts at: 3069 4043 3069
Size: 974 5765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5746 809
Size: 507 4430 1802
Fragments arranged by size:
4430 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3044 4186 5075
Size: 54 1425 50 275 18 1214 1142 889
Cuts at: 5075 6704 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1214 1142 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4186 1537
Size: 275 2374 4090
Fragments arranged by size:
4090 2374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2817 3182 3921 4669 1353
Size: 1464 365 739 748 3423
Fragments arranged by size:
3423 1464 748 739 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5326 5700 5846 387
Size: 1064 138 3737 374 146 1280
Fragments arranged by size:
3737 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2993 3163 3345 3776 4802 5399 6186
Size: 393 1361 170 182 431 1026 597 787
Cuts at: 6186 6454 1239
Size: 268 1524
Fragments arranged by size:
1524 1361 1026 787 597 431 393 268
182 170
EcoO109I rG'GnC_Cy
Cuts at: 2399 2399
Size: 6739
EcoRI G'AATT_C
Cuts at: 4048 4426 4048
Size: 378 6361
EcoRV GAT'ATC
Cuts at: 2986 3424 2986
Size: 438 6301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3234 3781 3860 3234
Size: 547 79 6113
Fragments arranged by size:
6113 547 79
FspI TGC'GCA
Cuts at: 5245 5245
Size: 6739
HaeII r_GCGC'y
Cuts at: 1678 5886 1678
Size: 4208 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2722 2754 5380 5412 5454
Size: 32 135 32 1150 32 2626 32 42
Cuts at: 5454 5486 1373
Size: 32 2626
Fragments arranged by size:
2626 2626 1150 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2047 4083 4329 669
Size: 988 281 109 2036 246 3079
Fragments arranged by size:
3079 2036 988 281 246 109
HindIII A'AGCT_T
Cuts at: 748 2536 748
Size: 1788 4951
HpaI GTT'AAC
Cuts at: 4329 4329
Size: 6739
KpnI G_GTAC'C
Cuts at: 4064 4064
Size: 6739
MfeI C'AATT_G
Cuts at: 4338 4338
Size: 6739
MluI A'CGCG_T
Cuts at: 6615 6615
Size: 6739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3528 5853 6037 1305
Size: 2223 2325 184 2007
Fragments arranged by size:
2325 2223 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4917
Fragments arranged by size:
4917 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3701 4697 5056 5215 518
Size: 648 2535 996 359 159 2042
Fragments arranged by size:
2535 2042 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3828 4155 4832 5980 6225 1606
Size: 2222 327 677 1148 245 2120
Fragments arranged by size:
2222 2120 1148 677 327 245
NcoI C'CATG_G
Cuts at: 513 1065 2258 513
Size: 552 1193 4994
Fragments arranged by size:
4994 1193 552
NdeI CA'TA_TG
Cuts at: 387 2544 387
Size: 2157 4582
NheI G'CTAG_C
Cuts at: 1052 3953 1052
Size: 2901 3838
NotI GC'GGCC_GC
Cuts at: 4186 4186
Size: 6739
NsiI A_TGCA'T
Cuts at: 3501 3501
Size: 6739
NspI r_CATG'y
Cuts at: 1132 1229 3322 4097 5642 1132
Size: 97 2093 775 1545 2229
Fragments arranged by size:
2229 2093 1545 775 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6739
PciI A'CATG_T
Cuts at: 5638 5638
Size: 6739
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6739
PfoI T'CCnGG_A
Cuts at: 2404 2404
Size: 6739
PmeI GTTT'AAAC
Cuts at: 4043 4043
Size: 6739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4808 4840 6321 6353 4808
Size: 32 1481 32 5194
Fragments arranged by size:
5194 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2399 2399
Size: 6739
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6739
PsiI TTA'TAA
Cuts at: 2759 4309 2759
Size: 1550 5189
PstI C_TGCA'G
Cuts at: 830 3865 4091 830
Size: 3035 226 3478
Fragments arranged by size:
3478 3035 226
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6739
SalI G'TCGA_C
Cuts at: 1936 4081 1936
Size: 2145 4594
SbfI CC_TGCA'GG
Cuts at: 4091 4091
Size: 6739
ScaI AGT'ACT
Cuts at: 4987 6699 4987
Size: 1712 5027
SexAI A'CCwGG_T
Cuts at: 2333 3908 2333
Size: 1575 5164
SfcI C'TryA_G
Cuts at: 826 1045 2901 3477 3861 4087 5222 5903 6094
Size: 219 1856 576 384 226 1135 681 191
Cuts at: 6094 826
Size: 1471
Fragments arranged by size:
1856 1471 1135 681 576 384 226 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6739
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6739
SmaI CCC'GGG
Cuts at: 1872 4066 1872
Size: 2194 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2122 2346 3888 4843 5744 6006
Size: 197 926 179 224 1542 955 901 262
Cuts at: 6006 820
Size: 1553
Fragments arranged by size:
1553 1542 955 926 901 262 224 197
179
SnaBI TAC'GTA
Cuts at: 493 4036 4530 493
Size: 3543 494 2702
Fragments arranged by size:
3543 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6739
SphI G_CATG'C
Cuts at: 1132 4097 1132
Size: 2965 3774
SspI AAT'ATT
Cuts at: 5 52 2842 4663 5
Size: 47 2790 1821 2081
Fragments arranged by size:
2790 2081 1821 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6739
StyI C'CwwG_G
Cuts at: 513 1065 2258 3047 3532 3979 4178 513
Size: 552 1193 789 485 447 199 3074
Fragments arranged by size:
3074 1193 789 552 485 447 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 3866 4766 4783 4936 5121 5566
Size: 1255 671 1615 900 17 153 185 445
Cuts at: 5566 325
Size: 1498
Fragments arranged by size:
1615 1498 1255 900 671 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2461 4985 6697
Size: 274 80 33 51 1443 484 2524 1712
Cuts at: 6697 96
Size: 138
Fragments arranged by size:
2524 1712 1443 484 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6699 6731 6699
Size: 32 6707
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6739
XbaI T'CTAG_A
Cuts at: 2068 2953 4075 2068
Size: 885 1122 4732
Fragments arranged by size:
4732 1122 885
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3149 3149
Size: 6739
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6739
XmaI C'CCGG_G
Cuts at: 1870 4064 1870
Size: 2194 4545
XmnI GAAnn'nnTTC
Cuts at: 4868 4868
Size: 6739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4547 276
Size: 53 83 186 936 3013 2468
Fragments arranged by size:
3013 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI