Restriction map for
pFN21ASDB0052
(Circular) (Six-base) MAPSORT of: pFN21ASDB0052.seq Check: 9016 from: 1 to: 6592
pFN21ASDB0052 6592 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2985 2985
Size: 6592
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3870 4402 278
Size: 53 83 186 936 2334 532 2468
Fragments arranged by size:
2468 2334 936 532 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3935 1937
Size: 1998 4594
Acc65I G'GTAC_C
Cuts at: 3913 3913
Size: 6592
AclI AA'CG_TT
Cuts at: 1563 4719 5092 1563
Size: 3156 373 3063
Fragments arranged by size:
3156 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2384 2825 3020 3245 4655 6039 6307 1632
Size: 752 441 195 225 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 752 441 268 225 195
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6395
AflIII A'CryG_T
Cuts at: 1203 5491 6468 1203
Size: 4288 977 1327
Fragments arranged by size:
4288 1327 977
AgeI A'CCGG_T
Cuts at: 5423 5423
Size: 6592
AhdI GACnn_n'nnGTC
Cuts at: 5321 5321
Size: 6592
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3304 3779 1184
Size: 2120 475 3997
Fragments arranged by size:
3997 2120 475
AlwNI CAG_nnn'CTG
Cuts at: 2748 2906 3107 3664 3859 4335 5907 2748
Size: 158 201 557 195 476 1572 3433
Fragments arranged by size:
3433 1572 557 476 201 195 158
ApaLI G'TGCA_C
Cuts at: 4649 5805 4649
Size: 1156 5436
ApoI r'AATT_y
Cuts at: 1493 2847 3901 4127 4279 6388 1493
Size: 1354 1054 226 152 2109 1697
Fragments arranged by size:
2109 1697 1354 1054 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5146 160
Size: 872 4114 1606
Fragments arranged by size:
4114 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
AvaI C'yCGr_G
Cuts at: 1870 1943 3790 3917 1870
Size: 73 1847 127 4545
Fragments arranged by size:
4545 1847 127 73
BamHI G'GATC_C
Cuts at: 3922 4289 3922
Size: 367 6225
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2973 3691 3913 5368
Size: 325 217 627 528 658 718 222 1455
Cuts at: 5368 618
Size: 1842
Fragments arranged by size:
1842 1455 718 658 627 528 325 222
217
BanII G_rGCy'C
Cuts at: 1391 2130 2433 3911 1391
Size: 739 303 1478 4072
Fragments arranged by size:
4072 1478 739 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3233 928
Size: 971 1334 4287
Fragments arranged by size:
4287 1334 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2297 2331 4783 4817 1903
Size: 34 360 34 2452 34 3678
Fragments arranged by size:
3678 2452 360 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4484 5694 4484
Size: 1210 5382
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6592
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5203 136
Size: 107 122 71 631 605 3531 1525
Fragments arranged by size:
3531 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6587 6587
Size: 6592
BlpI GC'TnA_GC
Cuts at: 4009 4009
Size: 6592
Bme1580I G_kGCm'C
Cuts at: 1790 2976 3694 4653 5809 1790
Size: 1186 718 959 1156 2573
Fragments arranged by size:
2573 1186 1156 959 718
BmgBI CAC'GTC
Cuts at: 1318 3559 1318
Size: 2241 4351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2287 3057 3607
Size: 416 53 83 399 24 870 770 550
Cuts at: 3607 4323 5281 442
Size: 716 958 1753
Fragments arranged by size:
1753 958 870 770 716 550 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 6592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2154 2363 2426 3063 3486 3670 3717
Size: 903 12 209 63 637 423 184 47
Cuts at: 3717 5252 1239
Size: 1535 2579
Fragments arranged by size:
2579 1535 903 637 423 209 184 63
47 12
Bpu10I CC'TnA_GC
Cuts at: 3276 3276
Size: 6592
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2241 4717 5582 5880 2241
Size: 2476 865 298 2953
Fragments arranged by size:
2953 2476 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2267 2436 2747 5255 882
Size: 617 768 169 311 2508 2219
Fragments arranged by size:
2508 2219 768 617 311 169
BsaAI yAC'GTr
Cuts at: 493 1204 3889 4383 493
Size: 711 2685 494 2702
Fragments arranged by size:
2702 2685 711 494
BsaBI GATnn'nnATC
Cuts at: 3392 6586 3392
Size: 3194 3398
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3786 3867 4399 4781
Size: 53 83 186 936 2253 81 532 382
Cuts at: 4781 5416 275
Size: 635 1451
Fragments arranged by size:
2253 1451 936 635 532 382 186 83
81 53
BsaWI w'CCGG_w
Cuts at: 3777 5025 5423 5697 5844 6255 3777
Size: 1248 398 274 147 411 4114
Fragments arranged by size:
4114 1248 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6367
Fragments arranged by size:
6367 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 3535 1186
Size: 1237 1112 4243
Fragments arranged by size:
4243 1237 1112
BseYI C'CCAG_C
Cuts at: 2218 2446 3751 5795 6291 2218
Size: 228 1305 2044 496 2519
Fragments arranged by size:
2519 2044 1305 496 228
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 2770 2867 3785 1163
Size: 573 569 465 97 918 3970
Fragments arranged by size:
3970 918 573 569 465 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4042 4803 5831 1540
Size: 275 186 2041 761 1028 2301
Fragments arranged by size:
2301 2041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 3039 4653 4738 5809 2130
Size: 909 1614 85 1071 2913
Fragments arranged by size:
2913 1614 1071 909 85
BsmI GAATG_Cn'
Cuts at: 3281 4103 4196 3281
Size: 822 93 5677
Fragments arranged by size:
5677 822 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3793 3793
Size: 6592
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2433 2976 3039 3694 3911 4653
Size: 399 340 303 543 63 655 217 742
Cuts at: 4653 4738 5809 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 543 399 340 303
217 85 63
BspEI T'CCGG_A
Cuts at: 3777 6255 3777
Size: 2478 4114
BspHI T'CATG_A
Cuts at: 3070 4481 6211 3070
Size: 1411 1730 3451
Fragments arranged by size:
3451 1730 1411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2441 2668 2985 3947 844
Size: 1597 227 317 962 3489
Fragments arranged by size:
3489 1597 962 317 227
BsrBI CCG'CTC
Cuts at: 1394 1823 3030 4479 1394
Size: 429 1207 1449 3507
Fragments arranged by size:
3507 1449 1207 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3579 5087 5261 65
Size: 1183 2331 1508 174 1396
Fragments arranged by size:
2331 1508 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2324 5236 5423 1700
Size: 110 514 2912 187 2869
Fragments arranged by size:
2912 2869 514 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6592
BssSI C'ACGA_G
Cuts at: 4652 5664 4652
Size: 1012 5580
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6592
BstBI TT'CG_AA
Cuts at: 2007 5396 2007
Size: 3389 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6592
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2241 2709 2146
Size: 95 468 6029
Fragments arranged by size:
6029 468 95
BstYI r'GATC_y
Cuts at: 1972 2875 3234 3922 4289 4674 4691 6143 6229
Size: 903 359 688 367 385 17 1452 86
Cuts at: 6229 6241 6587 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 903 688 385 367 359 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2042 3665 2042
Size: 1623 4969
BtgI C'CryG_G
Cuts at: 513 1065 2676 3873 513
Size: 552 1611 1197 3232
Fragments arranged by size:
3232 1611 1197 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2878 3532 487
Size: 683 56 1652 654 3547
Fragments arranged by size:
3547 1652 683 654 56
BtsI GCAGTG_nn'
Cuts at: 4108 4902 4922 4108
Size: 794 20 5778
Fragments arranged by size:
5778 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6592
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6557
DraI TTT'AAA
Cuts at: 3896 3896
Size: 6592
DraIII CAC_nnn'GTG
Cuts at: 3331 3331
Size: 6592
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3575 5599 809
Size: 507 2259 2024 1802
Fragments arranged by size:
2259 2024 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3333 4039 4928
Size: 54 1425 50 275 18 1503 706 889
Cuts at: 4928 6557 8
Size: 1629 43
Fragments arranged by size:
1629 1503 1425 889 706 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4039 1537
Size: 275 2227 4090
Fragments arranged by size:
4090 2227 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2434 3078 3346 4522 1353
Size: 1081 644 268 1176 3423
Fragments arranged by size:
3423 1176 1081 644 268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5179 5553 5699 387
Size: 1064 138 3590 374 146 1280
Fragments arranged by size:
3590 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 6592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2142 2154 2363 2384 2426 2825 3020
Size: 393 510 12 209 21 42 399 195
Cuts at: 3020 3063 3245 3486 3670 3717 4655 5252 6039
Size: 43 182 241 184 47 938 597 787
Cuts at: 6039 6307 1239
Size: 268 1524
Fragments arranged by size:
1524 938 787 597 510 399 393 268
241 209 195 184 182 47 43 42
21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2408 2408
Size: 6592
EcoO109I rG'GnC_Cy
Cuts at: 2046 2160 3301 3718 2046
Size: 114 1141 417 4920
Fragments arranged by size:
4920 1141 417 114
EcoRI G'AATT_C
Cuts at: 2847 3901 4279 2847
Size: 1054 378 5160
Fragments arranged by size:
5160 1054 378
FspI TGC'GCA
Cuts at: 3622 5098 3622
Size: 1476 5116
HaeII r_GCGC'y
Cuts at: 1678 5739 1678
Size: 4061 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2272 2304 5233 5265 5307
Size: 32 135 32 700 32 2929 32 42
Cuts at: 5307 5339 1373
Size: 32 2626
Fragments arranged by size:
2929 2626 700 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 3163 3409 3936 4182 669
Size: 988 281 259 966 246 527 246 3079
Fragments arranged by size:
3079 988 966 527 281 259 246 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6592
HpaI GTT'AAC
Cuts at: 4182 4182
Size: 6592
KpnI G_GTAC'C
Cuts at: 3917 3917
Size: 6592
MfeI C'AATT_G
Cuts at: 4191 4191
Size: 6592
MluI A'CGCG_T
Cuts at: 6468 6468
Size: 6592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2155 3658 5706 5890 1305
Size: 850 1503 2048 184 2007
Fragments arranged by size:
2048 2007 1503 850 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3335 10
Size: 54 1425 343 1503 3267
Fragments arranged by size:
3267 1503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3286 3540 3761 3809 4550 4909
Size: 648 1626 494 254 221 48 741 359
Cuts at: 4909 5068 518
Size: 159 2042
Fragments arranged by size:
2042 1626 741 648 494 359 254 221
159 48
MspA1I CmG'CkG
Cuts at: 1606 2362 2722 4008 4685 5833 6078 1606
Size: 756 360 1286 677 1148 245 2120
Fragments arranged by size:
2120 1286 1148 756 677 360 245
NcoI C'CATG_G
Cuts at: 513 1065 2676 513
Size: 552 1611 4429
Fragments arranged by size:
4429 1611 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6592
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6592
NotI GC'GGCC_GC
Cuts at: 4039 4039
Size: 6592
NspI r_CATG'y
Cuts at: 1132 1229 3950 5495 1132
Size: 97 2721 1545 2229
Fragments arranged by size:
2721 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2690 2954 1348
Size: 1342 264 4986
Fragments arranged by size:
4986 1342 264
PciI A'CATG_T
Cuts at: 5491 5491
Size: 6592
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3331 3859 1108
Size: 2223 528 3841
Fragments arranged by size:
3841 2223 528
PfoI T'CCnGG_A
Cuts at: 3448 3448
Size: 6592
PmeI GTTT'AAAC
Cuts at: 3896 3896
Size: 6592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4661 4693 6174 6206 4661
Size: 32 1481 32 5047
Fragments arranged by size:
5047 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2160 3301 2046
Size: 114 1141 5337
Fragments arranged by size:
5337 1141 114
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6592
PsiI TTA'TAA
Cuts at: 4162 4162
Size: 6592
PstI C_TGCA'G
Cuts at: 830 2396 3132 3944 830
Size: 1566 736 812 3478
Fragments arranged by size:
3478 1566 812 736
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6592
PvuII CAG'CTG
Cuts at: 2362 2362
Size: 6592
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 6592
SalI G'TCGA_C
Cuts at: 1936 3934 1936
Size: 1998 4594
SanDI GG'GwC_CC
Cuts at: 3301 3301
Size: 6592
SapI GCTCTTCn'nnn_
Cuts at: 3346 3346
Size: 6592
SbfI CC_TGCA'GG
Cuts at: 3132 3944 3132
Size: 812 5780
ScaI AGT'ACT
Cuts at: 2351 4840 6552 2351
Size: 2489 1712 2391
Fragments arranged by size:
2489 2391 1712
SexAI A'CCwGG_T
Cuts at: 2824 3327 2824
Size: 503 6089
SfcI C'TryA_G
Cuts at: 826 1045 2392 2887 3128 3940 5075 5756 5947
Size: 219 1347 495 241 812 1135 681 191
Cuts at: 5947 826
Size: 1471
Fragments arranged by size:
1471 1347 1135 812 681 495 241 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6592
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6592
SmaI CCC'GGG
Cuts at: 1872 3919 1872
Size: 2047 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2256 4696 5597 5859 820
Size: 197 926 313 2440 901 262 1553
Fragments arranged by size:
2440 1553 926 901 313 262 197
SnaBI TAC'GTA
Cuts at: 493 3889 4383 493
Size: 3396 494 2702
Fragments arranged by size:
3396 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6592
SphI G_CATG'C
Cuts at: 1132 3950 1132
Size: 2818 3774
SspI AAT'ATT
Cuts at: 5 52 4516 5
Size: 47 4464 2081
Fragments arranged by size:
4464 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 3005 3134 3707 1517
Size: 897 591 129 573 4402
Fragments arranged by size:
4402 897 591 573 129
StyI C'CwwG_G
Cuts at: 513 1065 2415 2547 2676 3135 3253 4031 513
Size: 552 1350 132 129 459 118 778 3074
Fragments arranged by size:
3074 1350 778 552 459 132 129 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3718 3851 4619 4636 4789 4974 5419
Size: 1255 2138 133 768 17 153 185 445
Cuts at: 5419 325
Size: 1498
Fragments arranged by size:
2138 1498 1255 768 445 185 153 133
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2349 3636 4838
Size: 274 80 33 51 1443 372 1287 1202
Cuts at: 4838 6550 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1287 1202 372 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2030 2062 3615 3647 6552 6584 2030
Size: 32 1553 32 2905 32 2038
Fragments arranged by size:
2905 2038 1553 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6592
XbaI T'CTAG_A
Cuts at: 3928 3928
Size: 6592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2554 2198
Size: 356 6236
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6592
XmaI C'CCGG_G
Cuts at: 1870 3917 1870
Size: 2047 4545
XmnI GAAnn'nnTTC
Cuts at: 4721 4721
Size: 6592
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3868 4400 276
Size: 53 83 186 936 2334 532 2468
Fragments arranged by size:
2468 2334 936 532 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI