Restriction map for
pFN21ASDB0050
(Circular) (Six-base) MAPSORT of: pFN21ASDB0050.seq Check: 6852 from: 1 to: 8599
pFN21ASDB0050 8599 bp
With 182 enzymes: SgfI *
January 16, 2009 15:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3934 3934
Size: 8599
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6409 278
Size: 53 83 186 936 4873 2468
Fragments arranged by size:
4873 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4896 5942 1937
Size: 2959 1046 4594
Fragments arranged by size:
4594 2959 1046
Acc65I G'GTAC_C
Cuts at: 4026 5920 4026
Size: 1894 6705
AclI AA'CG_TT
Cuts at: 1563 6726 7099 1563
Size: 5163 373 3063
Fragments arranged by size:
5163 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 4857 5009 5591 5674 6662 8046 8314
Size: 941 2284 152 582 83 988 1384 268
Cuts at: 8314 1632
Size: 1917
Fragments arranged by size:
2284 1917 1384 988 941 582 268 152
83
AflII C'TTAA_G
Cuts at: 820 1017 3386 820
Size: 197 2369 6033
Fragments arranged by size:
6033 2369 197
AflIII A'CryG_T
Cuts at: 1203 2415 3030 4959 5517 7498 8475 1203
Size: 1212 615 1929 558 1981 977 1327
Fragments arranged by size:
1981 1929 1327 1212 977 615 558
AgeI A'CCGG_T
Cuts at: 7430 7430
Size: 8599
AhdI GACnn_n'nnGTC
Cuts at: 7328 7328
Size: 8599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2982 3668 1184
Size: 1798 686 6115
Fragments arranged by size:
6115 1798 686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 2549
Size: 32 8567
AlwNI CAG_nnn'CTG
Cuts at: 3989 5074 5199 6342 7914 3989
Size: 1085 125 1143 1572 4674
Fragments arranged by size:
4674 1572 1143 1085 125
ApaI G_GGCC'C
Cuts at: 2064 4342 5314 5857 2064
Size: 2278 972 543 4806
Fragments arranged by size:
4806 2278 972 543
ApaLI G'TGCA_C
Cuts at: 6656 7812 6656
Size: 1156 7443
ApoI r'AATT_y
Cuts at: 1493 2363 5480 5533 5908 6134 6286 8395 1493
Size: 870 3117 53 375 226 152 2109 1697
Fragments arranged by size:
3117 2109 1697 870 375 226 152 53
AscI GG'CGCG_CC
Cuts at: 2067 2067
Size: 8599
AseI AT'TA_AT
Cuts at: 160 1032 7153 160
Size: 872 6121 1606
Fragments arranged by size:
6121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8599
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 2159 5754 5868 5924 1870
Size: 73 165 51 3595 114 56 4545
Fragments arranged by size:
4545 3595 165 114 73 56 51
AvrII C'CTAG_G
Cuts at: 4624 4624
Size: 8599
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3832 3865 3832
Size: 33 8566
BamHI G'GATC_C
Cuts at: 5929 6296 5929
Size: 367 8232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2117 2216 2461 3445
Size: 325 217 627 243 87 99 245 984
Cuts at: 3445 3946 4026 5920 7375 618
Size: 501 80 1894 1455 1842
Fragments arranged by size:
1894 1842 1455 984 627 501 325 245
243 217 99 87 80
BanII G_rGCy'C
Cuts at: 1391 2064 3369 3627 3757 4342 4881 5314 5720
Size: 673 1305 258 130 585 539 433 406
Cuts at: 5720 5755 5857 5918 1391
Size: 35 102 61 4072
Fragments arranged by size:
4072 1305 673 585 539 433 406 258
130 102 61 35
BbeI G_GCGC'C
Cuts at: 2034 2220 2034
Size: 186 8413
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 2282 2705 4702 5408 5520 5579
Size: 971 275 108 423 1997 706 112 59
Cuts at: 5579 928
Size: 3948
Fragments arranged by size:
3948 1997 971 706 423 275 112 108
59
BbvCI CC'TCA_GC
Cuts at: 3423 4146 3423
Size: 723 7876
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6790 6824 1903
Size: 34 4853 34 3678
Fragments arranged by size:
4853 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2443 3549 3860 5573 6491 7701 2443
Size: 1106 311 1713 918 1210 3341
Fragments arranged by size:
3341 1713 1210 1106 918 311
BclI T'GATC_A
Cuts at: 1548 5640 1548
Size: 4092 4507
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3230 7210 136
Size: 107 122 71 631 605 1558 3980 1525
Fragments arranged by size:
3980 1558 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3351 3570 8594 3351
Size: 219 5024 3356
Fragments arranged by size:
5024 3356 219
BlpI GC'TnA_GC
Cuts at: 2381 2664 2855 3717 4056 4323 6016 2381
Size: 283 191 862 339 267 1693 4964
Fragments arranged by size:
4964 1693 862 339 283 267 191
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2120 3168 3951 4342 5314 5857 6660
Size: 274 56 1048 783 391 972 543 803
Cuts at: 6660 7816 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1048 972 803 783 543 391
274 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8599
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3239 4795 5552
Size: 416 53 83 399 24 1822 1556 757
Cuts at: 5552 6330 7288 442
Size: 778 958 1753
Fragments arranged by size:
1822 1753 1556 958 778 757 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3673 5647 5734 3673
Size: 1974 87 6538
Fragments arranged by size:
6538 1974 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2645 2819 3040 3533 4325 4607 4895
Size: 968 438 174 221 493 792 282 288
Cuts at: 4895 5297 5394 5642 7259 1239
Size: 402 97 248 1617 2579
Fragments arranged by size:
2579 1617 968 792 493 438 402 288
282 248 221 174 97
Bpu10I CC'TnA_GC
Cuts at: 3423 4146 5544 3423
Size: 723 1398 6478
Fragments arranged by size:
6478 1398 723
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6724 7589 7887 6724
Size: 865 298 7436
Fragments arranged by size:
7436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2862 3581 3740 3782 7262 882
Size: 617 1363 719 159 42 3480 2219
Fragments arranged by size:
3480 2219 1363 719 617 159 42
BsaAI yAC'GTr
Cuts at: 493 1204 2205 3033 4558 4996 5209 5896 6390
Size: 711 1001 828 1525 438 213 687 494
Cuts at: 6390 493
Size: 2702
Fragments arranged by size:
2702 1525 1001 828 711 687 494 438
213
BsaBI GATnn'nnATC
Cuts at: 2298 2520 3401 4673 8593 2298
Size: 222 881 1272 3920 2304
Fragments arranged by size:
3920 2304 1272 881 222
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2079 2217 3706
Size: 53 83 186 936 498 48 138 1489
Cuts at: 3706 4727 4824 6406 6788 7423 275
Size: 1021 97 1582 382 635 1451
Fragments arranged by size:
1582 1489 1451 1021 936 635 498 382
186 138 97 83 53 48
BsaWI w'CCGG_w
Cuts at: 2493 3749 7032 7430 7704 7851 8262 2493
Size: 1256 3283 398 274 147 411 2830
Fragments arranged by size:
3283 2830 1256 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2999 3029 3038 3068 1178
Size: 30 165 30 1596 30 9 30 6709
Fragments arranged by size:
6709 1596 165 30 30 30 30 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2903 3007 3010 3323 3455 3599 3632 3635
Size: 1717 104 3 313 132 144 33 3
Cuts at: 3635 4310 4787 1186
Size: 675 477 4998
Fragments arranged by size:
4998 1717 675 477 313 144 132 104
33 3 3
BseYI C'CCAG_C
Cuts at: 2311 2741 3126 3282 3319 3869 5305 7802 8298
Size: 430 385 156 37 550 1436 2497 496
Cuts at: 8298 2311
Size: 2612
Fragments arranged by size:
2612 2497 1436 550 496 430 385 156
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2075 2861 3188 3638 4425 1163
Size: 573 339 786 327 450 787 5337
Fragments arranged by size:
5337 787 786 573 450 339 327
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2324 2753 6049 6810 7838 1540
Size: 275 186 323 429 3296 761 1028 2301
Fragments arranged by size:
3296 2301 1028 761 429 323 275 186
BsiHKAI G_wGCw'C
Cuts at: 4051 4062 4605 4881 6660 6745 7816 4051
Size: 11 543 276 1779 85 1071 4834
Fragments arranged by size:
4834 1779 1071 543 276 85 11
BsmI GAATG_Cn'
Cuts at: 6110 6203 6110
Size: 93 8506
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2183
Size: 8599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2120 3168 3369 3627 3757 3951
Size: 399 274 56 1048 201 258 130 194
Cuts at: 3951 4051 4062 4342 4605 4881 5314 5720 5755
Size: 100 11 280 263 276 433 406 35
Cuts at: 5755 5857 5918 6660 6745 7816 1391
Size: 102 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 1048 742 433 406 399 280
276 274 263 258 201 194 130 102
100 85 61 56 35 11
BspEI T'CCGG_A
Cuts at: 2493 8262 2493
Size: 5769 2830
BspHI T'CATG_A
Cuts at: 2124 5184 6488 8218 2124
Size: 3060 1304 1730 2505
Fragments arranged by size:
3060 2505 1730 1304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3934 5954 844
Size: 3090 2020 3489
Fragments arranged by size:
3489 3090 2020
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 2611 2749 5713 6486 1394
Size: 429 341 447 138 2964 773 3507
Fragments arranged by size:
3507 2964 773 447 429 341 138
BsrDI GCAATG_nn'
Cuts at: 65 1248 2335 2453 3276 4162 7094 7268 65
Size: 1183 1087 118 823 886 2932 174 1396
Fragments arranged by size:
2932 1396 1183 1087 886 823 174 118
BsrFI r'CCGG_y
Cuts at: 1700 1810 4657 4698 4902 7243 7430 1700
Size: 110 2847 41 204 2341 187 2869
Fragments arranged by size:
2869 2847 2341 204 187 110 41
BsrGI T'GTAC_A
Cuts at: 96 2685 96
Size: 2589 6010
BssHII G'CGCG_C
Cuts at: 1925 2067 1925
Size: 142 8457
BssSI C'ACGA_G
Cuts at: 3773 4010 4080 6659 7671 3773
Size: 237 70 2579 1012 4701
Fragments arranged by size:
4701 2579 1012 237 70
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8599
BstBI TT'CG_AA
Cuts at: 2007 7403 2007
Size: 5396 3203
BstEII G'GTnAC_C
Cuts at: 1184 5675 1184
Size: 4491 4108
BstXI CCAn_nnnn'nTGG
Cuts at: 3346 4723 3346
Size: 1377 7222
BstYI r'GATC_y
Cuts at: 1972 2262 3012 3351 3396 3570 4797 5109 5929
Size: 290 750 339 45 174 1227 312 820
Cuts at: 5929 6296 6681 6698 8150 8236 8248 8594 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1227 820 750 385 367 346
339 312 290 174 86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 4683 5728 4683
Size: 1045 7554
BtgI C'CryG_G
Cuts at: 513 1065 2022 3160 3345 3676 4578 5269 513
Size: 552 957 1138 185 331 902 691 3843
Fragments arranged by size:
3843 1138 957 902 691 552 331 185
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3697 487
Size: 683 56 2471 5389
Fragments arranged by size:
5389 2471 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 3436 3601 3841 5595 6115 6909 6929 3268
Size: 168 165 240 1754 520 794 20 4938
Fragments arranged by size:
4938 1754 794 520 240 168 165 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4539 4574 529
Size: 35 3975 35 4554
Fragments arranged by size:
4554 3975 35 35
DraI TTT'AAA
Cuts at: 5903 5903
Size: 8599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3902 7606 809
Size: 507 2586 3704 1802
Fragments arranged by size:
3704 2586 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2321 2750 5332
Size: 54 1425 50 275 18 491 429 2582
Cuts at: 5332 5441 5746 6046 6935 8564 8
Size: 109 305 300 889 1629 43
Fragments arranged by size:
2582 1629 1425 889 491 429 305 300
275 109 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2321 2750 6046 1537
Size: 275 509 429 3296 4090
Fragments arranged by size:
4090 3296 509 429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4557 4709 4917 5186 6529 1353
Size: 3204 152 208 269 1343 3423
Fragments arranged by size:
3423 3204 1343 269 208 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2255 3838 7186 7560 7706 387
Size: 1064 138 666 1583 3348 374 146 1280
Fragments arranged by size:
3348 1583 1280 1064 666 374 146 138
EcoICRI GAG'CTC
Cuts at: 4879 4879
Size: 8599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2573 2645 2819 3040 3533 4325
Size: 393 575 366 72 174 221 493 792
Cuts at: 4325 4607 4857 4895 5009 5297 5394 5591 5642
Size: 282 250 38 114 288 97 197 51
Cuts at: 5642 5674 6662 7259 8046 8314 1239
Size: 32 988 597 787 268 1524
Fragments arranged by size:
1524 988 792 787 597 575 493 393
366 288 282 268 250 221 197 174
114 97 72 51 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 4346 5543 4346
Size: 1197 7402
EcoO109I rG'GnC_Cy
Cuts at: 2308 2548 2584 3517 3873 4089 4338 4339 5176
Size: 240 36 933 356 216 249 1 837
Cuts at: 5176 5310 5617 5853 5854 2308
Size: 134 307 236 1 5053
Fragments arranged by size:
5053 933 837 356 307 249 240 236
216 134 36 1 1
EcoRI G'AATT_C
Cuts at: 5908 6286 5908
Size: 378 8221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3904 3904
Size: 8599
FspI TGC'GCA
Cuts at: 7105 7105
Size: 8599
HaeII r_GCGC'y
Cuts at: 1678 2034 2220 2694 3724 4470 7746 1678
Size: 356 186 474 1030 746 3276 2531
Fragments arranged by size:
3276 2531 1030 746 474 356 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 3038 3070 3088
Size: 32 135 32 950 32 484 32 18
Cuts at: 3088 3120 4949 4981 5028 5060 7240 7272 7314
Size: 32 1829 32 47 32 2180 32 42
Cuts at: 7314 7346 1373
Size: 32 2626
Fragments arranged by size:
2626 2180 1829 950 484 135 47 42
32 32 32 32 32 32 32 32
32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 2575 4228 4573 5943 6189 669
Size: 988 281 637 1653 345 1370 246 3079
Fragments arranged by size:
3079 1653 1370 988 637 345 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8599
HpaI GTT'AAC
Cuts at: 6189 6189
Size: 8599
KasI G'GCGC_C
Cuts at: 2030 2216 2030
Size: 186 8413
KpnI G_GTAC'C
Cuts at: 4030 5924 4030
Size: 1894 6705
MfeI C'AATT_G
Cuts at: 6198 6198
Size: 8599
MluI A'CGCG_T
Cuts at: 8475 8475
Size: 8599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3099 5109 7713 7897 1305
Size: 1794 2010 2604 184 2007
Fragments arranged by size:
2604 2010 2007 1794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5443 10
Size: 54 1425 343 3611 3166
Fragments arranged by size:
3611 3166 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 3660 4067 4167 4721 6557 6916
Size: 648 957 1537 407 100 554 1836 359
Cuts at: 6916 7075 518
Size: 159 2042
Fragments arranged by size:
2042 1836 1537 957 648 554 407 359
159 100
MspA1I CmG'CkG
Cuts at: 1606 2637 2668 2962 4322 4537 5271 5737 6015
Size: 1031 31 294 1360 215 734 466 278
Cuts at: 6015 6692 7840 8085 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1360 1148 1031 734 677 466 294
278 245 215 31
NaeI GCC'GGC
Cuts at: 4659 4904 4659
Size: 245 8354
NarI GG'CG_CC
Cuts at: 2031 2217 2031
Size: 186 8413
NcoI C'CATG_G
Cuts at: 513 1065 2022 3160 3676 513
Size: 552 957 1138 516 5436
Fragments arranged by size:
5436 1138 957 552 516
NdeI CA'TA_TG
Cuts at: 387 4126 387
Size: 3739 4860
NgoMIV G'CCGG_C
Cuts at: 4657 4902 4657
Size: 245 8354
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8599
NotI GC'GGCC_GC
Cuts at: 6046 6046
Size: 8599
NspI r_CATG'y
Cuts at: 1132 1229 2419 2563 2596 3224 4066 4963 5521
Size: 97 1190 144 33 628 842 897 558
Cuts at: 5521 5957 7502 1132
Size: 436 1545 2229
Fragments arranged by size:
2229 1545 1190 897 842 628 558 436
144 97 33
PasI CC'CwG_GG
Cuts at: 1348 2270 1348
Size: 922 7677
PciI A'CATG_T
Cuts at: 2415 4959 5517 7498 2415
Size: 2544 558 1981 3516
Fragments arranged by size:
3516 2544 1981 558
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4483 5074 5330 1108
Size: 3375 591 256 4377
Fragments arranged by size:
4377 3375 591 256
PfoI T'CCnGG_A
Cuts at: 2904 4971 2904
Size: 2067 6532
PmeI GTTT'AAAC
Cuts at: 5903 5903
Size: 8599
PmlI CAC'GTG
Cuts at: 2205 2205
Size: 8599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3656 3688 6668 6700 8181 8213 3656
Size: 32 2980 32 1481 32 4042
Fragments arranged by size:
4042 2980 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2308 2548 2584 3517 3873 5617 2308
Size: 240 36 933 356 1744 5290
Fragments arranged by size:
5290 1744 933 356 240 36
PshAI GACnn'nnGTC
Cuts at: 1613 3705 5682 5870 1613
Size: 2092 1977 188 4342
Fragments arranged by size:
4342 2092 1977 188
PsiI TTA'TAA
Cuts at: 6169 6169
Size: 8599
PspOMI G'GGCC_C
Cuts at: 2060 4338 5310 5853 2060
Size: 2278 972 543 4806
Fragments arranged by size:
4806 2278 972 543
PspXI vC'TCGA_Gb
Cuts at: 2108 2108
Size: 8599
PstI C_TGCA'G
Cuts at: 830 2478 3275 4782 5043 5217 5951 830
Size: 1648 797 1507 261 174 734 3478
Fragments arranged by size:
3478 1648 1507 797 734 261 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8599
PvuII CAG'CTG
Cuts at: 2668 2962 4322 4537 5737 2668
Size: 294 1360 215 1200 5530
Fragments arranged by size:
5530 1360 1200 294 215
SacI G_AGCT'C
Cuts at: 4881 4881
Size: 8599
SacII CC_GC'GG
Cuts at: 5272 5272
Size: 8599
SalI G'TCGA_C
Cuts at: 1936 5941 1936
Size: 4005 4594
SanDI GG'GwC_CC
Cuts at: 2584 3873 2584
Size: 1289 7310
SapI GCTCTTCn'nnn_
Cuts at: 4709 4709
Size: 8599
SbfI CC_TGCA'GG
Cuts at: 5951 5951
Size: 8599
ScaI AGT'ACT
Cuts at: 2731 3737 6847 8559 2731
Size: 1006 3110 1712 2771
Fragments arranged by size:
3110 2771 1712 1006
SfcI C'TryA_G
Cuts at: 826 1045 2474 3271 4778 5039 5213 5608 5947
Size: 219 1429 797 1507 261 174 395 339
Cuts at: 5947 7082 7763 7954 826
Size: 1135 681 191 1471
Fragments arranged by size:
1507 1471 1429 1135 797 681 395 339
261 219 191 174
SfoI GGC'GCC
Cuts at: 2032 2218 2032
Size: 186 8413
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8599
SmaI CCC'GGG
Cuts at: 1872 5756 5926 1872
Size: 3884 170 4545
Fragments arranged by size:
4545 3884 170
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 3386 6703 7604 7866 820
Size: 197 926 165 1278 3317 901 262 1553
Fragments arranged by size:
3317 1553 1278 926 901 262 197 165
SnaBI TAC'GTA
Cuts at: 493 5896 6390 493
Size: 5403 494 2702
Fragments arranged by size:
5403 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8599
SphI G_CATG'C
Cuts at: 1132 3224 5957 1132
Size: 2092 2733 3774
Fragments arranged by size:
3774 2733 2092
SspI AAT'ATT
Cuts at: 5 52 6523 5
Size: 47 6471 2081
Fragments arranged by size:
6471 2081 47
StuI AGG'CCT
Cuts at: 1517 5240 5568 1517
Size: 3723 328 4548
Fragments arranged by size:
4548 3723 328
StyI C'CwwG_G
Cuts at: 513 1065 2022 2544 3069 3160 3627 3676 4624
Size: 552 957 522 525 91 467 49 948
Cuts at: 4624 4980 5061 5664 6038 513
Size: 356 81 603 374 3074
Fragments arranged by size:
3074 957 948 603 552 525 522 467
374 356 91 81 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2479 3391 4777 6626 6643 6796 6981
Size: 1255 899 912 1386 1849 17 153 185
Cuts at: 6981 7426 325
Size: 445 1498
Fragments arranged by size:
1849 1498 1386 1255 912 899 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2685 2729 3462
Size: 274 80 33 51 1443 708 44 733
Cuts at: 3462 3735 5028 6845 8557 96
Size: 273 1293 1817 1712 138
Fragments arranged by size:
1817 1712 1443 1293 733 708 274 273
138 80 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8559 8591 8559
Size: 32 8567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8599
XbaI T'CTAG_A
Cuts at: 5935 5935
Size: 8599
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5741 5741
Size: 8599
XhoI C'TCGA_G
Cuts at: 1943 2108 1943
Size: 165 8434
XmaI C'CCGG_G
Cuts at: 1870 5754 5924 1870
Size: 3884 170 4545
Fragments arranged by size:
4545 3884 170
XmnI GAAnn'nnTTC
Cuts at: 2171 3671 6728 2171
Size: 1500 3057 4042
Fragments arranged by size:
4042 3057 1500
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6407 276
Size: 53 83 186 936 4873 2468
Fragments arranged by size:
4873 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI
NruI NsiI PacI PsrI RsrII SexAI SfiI SrfI
SwaI